mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    IMDM no phenol red
    Description:
    Iscove s Modified Dulbecco s Medium IMDM is well suited for rapidly proliferating high density cell cultures including Jurkat COS 7 and macrophage cells We offer a variety of Gibco IMDM modifications for a range of cell culture applications Find the right formulation using the media selector tool This IMDM is modified as follows With Without • L glutamine • Phenol Red • α thioglycerol • 2 mercaptoethanol The complete formulation is available IMDM a modification of Dulbecco s Modified Eagle Medium includes selenium as well as additional amino acids and vitamins In addition this unique medium lacks iron with potassium nitrate replacing ferric nitrate Product UseFor Research Use Only Not intended for animal or human diagnostic or therapeutic use cGMP Manufacturing and Quality SystemGibco IMDM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards IMDM contains no proteins lipids or growth factors Therefore IMDM requires supplementation commonly with 10 Fetal Bovine Serum FBS IMDM uses a sodium bicarbonate buffer system 3 024 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH
    Catalog Number:
    21056023
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
    Buy from Supplier


    Structured Review

    Thermo Fisher mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm
    Iscove s Modified Dulbecco s Medium IMDM is well suited for rapidly proliferating high density cell cultures including Jurkat COS 7 and macrophage cells We offer a variety of Gibco IMDM modifications for a range of cell culture applications Find the right formulation using the media selector tool This IMDM is modified as follows With Without • L glutamine • Phenol Red • α thioglycerol • 2 mercaptoethanol The complete formulation is available IMDM a modification of Dulbecco s Modified Eagle Medium includes selenium as well as additional amino acids and vitamins In addition this unique medium lacks iron with potassium nitrate replacing ferric nitrate Product UseFor Research Use Only Not intended for animal or human diagnostic or therapeutic use cGMP Manufacturing and Quality SystemGibco IMDM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards IMDM contains no proteins lipids or growth factors Therefore IMDM requires supplementation commonly with 10 Fetal Bovine Serum FBS IMDM uses a sodium bicarbonate buffer system 3 024 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH
    https://www.bioz.com/result/mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm - by Bioz Stars, 2021-03
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Murine Cardiosphere-Derived Cells Are Impaired by Age but Not by Cardiac Dystrophic Dysfunction
    Article Snippet: Briefly, CDCs at passage 2 were seeded into a 96-well plate at a density of 500 cells/well and allowed to attach overnight. .. The cells were washed twice with PBS and incubated with 10% AlamarBlue, diluted in phenol red-free Iscove's modified Dulbecco's medium (IMDM; Invitrogen), at 37°C. .. The relative fluorescence intensity at 530–560 nm excitation wavelength and 590 nm emission wavelength, quantified by FLUOstar OPTIMA (BMG Labtech), was directly related to the number of live cells using a standard curve.

    Article Title: A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis
    Article Snippet: Live-cell imaging of different lineages was performed on a confocal laser scanning microscope TCS SP8 (Leica) at 37 °C and 5% CO2 . .. Prior to imaging, freshly isolated neutrophils were incubated for 3 h at 37 °C in phenol red-free IMDM (Gibco) supplemented with 2.5% autologous serum and 20 µM R-roscovitine (Selleckchem). .. UV-treated BL-2 cells were incubated for 3 h at 37 °C prior to imaging.

    Modification:

    Article Title: Murine Cardiosphere-Derived Cells Are Impaired by Age but Not by Cardiac Dystrophic Dysfunction
    Article Snippet: Briefly, CDCs at passage 2 were seeded into a 96-well plate at a density of 500 cells/well and allowed to attach overnight. .. The cells were washed twice with PBS and incubated with 10% AlamarBlue, diluted in phenol red-free Iscove's modified Dulbecco's medium (IMDM; Invitrogen), at 37°C. .. The relative fluorescence intensity at 530–560 nm excitation wavelength and 590 nm emission wavelength, quantified by FLUOstar OPTIMA (BMG Labtech), was directly related to the number of live cells using a standard curve.

    Article Title: Role of the Nuclear Receptor Coactivator AIB1-?4 Splice Variant in the Control of Gene Transcription *
    Article Snippet: CHO cells were grown in DMEM F-12 (Invitrogen) with 10% FBS. .. HEK293 cells were grown in phenol red-free Iscove's modified Eagle's medium (IMEM, Invitrogen) with 10% charcoal-stripped serum (CCS). ..

    Article Title: Host Cathelicidin Exacerbates Group B Streptococcus Urinary Tract Infection
    Article Snippet: The human bladder epithelial cell line 5637 (ATCC HTB-9) was cultured in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) at 37°C in humidified air with 5% CO2 . .. The human mast cell line HMC-1 ( ) was cultured in Iscove's modified Dulbecco's medium (IMDM) (catalog no. 21056-023; Gibco) supplemented with 1.2 mM 1 -thioglycerol (M6145-100ML; Sigma-Aldrich) and 10% FBS at 37°C in humidified air with 5% CO2. .. For LL-37 stimulation assays, HTB-9 cells were infected as described previously ( ).

    Imaging:

    Article Title: A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis
    Article Snippet: Live-cell imaging of different lineages was performed on a confocal laser scanning microscope TCS SP8 (Leica) at 37 °C and 5% CO2 . .. Prior to imaging, freshly isolated neutrophils were incubated for 3 h at 37 °C in phenol red-free IMDM (Gibco) supplemented with 2.5% autologous serum and 20 µM R-roscovitine (Selleckchem). .. UV-treated BL-2 cells were incubated for 3 h at 37 °C prior to imaging.

    Article Title: Uncoupling of DNA Replication and Centrosome Duplication Cycles Is a Primary Cause of Haploid Instability in Mammalian Somatic Cells
    Article Snippet: For immunofluorescence imaging, cells were fixed and stained 8 h after the release from the third inhibitor treatment. .. For live imaging, the cell culture medium was exchanged to supplemented phenol red-free IMDM (Thermo Fisher Scientific), and cells were imaged from 2 h after the release from the third inhibitor treatment. .. For monitoring the dynamics of DNA content in the long-term culture experiments, cells were subjected to flow cytometry 8 h after release from inhibitor treatment at the cycles of inhibitor treatment/release indicated in the main text and the corresponding figure.

    Isolation:

    Article Title: A fluorogenic cyclic peptide for imaging and quantification of drug-induced apoptosis
    Article Snippet: Live-cell imaging of different lineages was performed on a confocal laser scanning microscope TCS SP8 (Leica) at 37 °C and 5% CO2 . .. Prior to imaging, freshly isolated neutrophils were incubated for 3 h at 37 °C in phenol red-free IMDM (Gibco) supplemented with 2.5% autologous serum and 20 µM R-roscovitine (Selleckchem). .. UV-treated BL-2 cells were incubated for 3 h at 37 °C prior to imaging.

    Concentration Assay:

    Article Title: Role of N-terminal protein formylation in central metabolic processes in Staphylococcus aureus
    Article Snippet: .. Minimal inhibitory concentration of antibiotics To define differences in the susceptibility to trimethoprim and sulfamethoxazole (Sigma) over-night cultures of RN4220 wild type, Δfmt, and the complemented mutant were used to inoculate 500 μl IMDM without phenol red (Gibco) to an OD578 of 0.1 in 24-well plates (Costar) containing serially diluted antibiotics in duplicates. ..

    Mutagenesis:

    Article Title: Role of N-terminal protein formylation in central metabolic processes in Staphylococcus aureus
    Article Snippet: .. Minimal inhibitory concentration of antibiotics To define differences in the susceptibility to trimethoprim and sulfamethoxazole (Sigma) over-night cultures of RN4220 wild type, Δfmt, and the complemented mutant were used to inoculate 500 μl IMDM without phenol red (Gibco) to an OD578 of 0.1 in 24-well plates (Costar) containing serially diluted antibiotics in duplicates. ..

    Activity Assay:

    Article Title: Luminescent multiplex viability assay for Trypanosoma brucei gambiense
    Article Snippet: HMI – human serum – methylcellulose (HHM) is HH containing a final concentration of 1.1% w/v methylcellulose. .. For fluorescent and luminescent activity assays, HH was prepared from IMDM without phenol red (Invitrogen). .. T. b. gambiense MHOM/CD/INRB/2006/23A T. b. gambiense strain MHOM/CD/INRB/2006/23A, alias 348 BT, was isolated in Mbuyi-Mayi in the Democratic Republic of the Congo in 2006, from the blood of a second stage patient who was cured after a 10 day melarsoprol treatment [ ].

    Cell Culture:

    Article Title: Host Cathelicidin Exacerbates Group B Streptococcus Urinary Tract Infection
    Article Snippet: The human bladder epithelial cell line 5637 (ATCC HTB-9) was cultured in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) at 37°C in humidified air with 5% CO2 . .. The human mast cell line HMC-1 ( ) was cultured in Iscove's modified Dulbecco's medium (IMDM) (catalog no. 21056-023; Gibco) supplemented with 1.2 mM 1 -thioglycerol (M6145-100ML; Sigma-Aldrich) and 10% FBS at 37°C in humidified air with 5% CO2. .. For LL-37 stimulation assays, HTB-9 cells were infected as described previously ( ).

    Article Title: Uncoupling of DNA Replication and Centrosome Duplication Cycles Is a Primary Cause of Haploid Instability in Mammalian Somatic Cells
    Article Snippet: For immunofluorescence imaging, cells were fixed and stained 8 h after the release from the third inhibitor treatment. .. For live imaging, the cell culture medium was exchanged to supplemented phenol red-free IMDM (Thermo Fisher Scientific), and cells were imaged from 2 h after the release from the third inhibitor treatment. .. For monitoring the dynamics of DNA content in the long-term culture experiments, cells were subjected to flow cytometry 8 h after release from inhibitor treatment at the cycles of inhibitor treatment/release indicated in the main text and the corresponding figure.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm
    Mononuclear Cell Mnc Complete Medium Complete Medium Mnc Basal Medium Complete Medium Mnc Basal Medium Imdm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mononuclear cell mnc complete medium complete medium mnc basal medium complete medium mnc basal medium imdm - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    Image Search Results