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monoclonal rat anti hcn4  (Novus Biologicals)


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    Novus Biologicals monoclonal rat anti hcn4
    Monoclonal Rat Anti Hcn4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rat anti hcn4/product/Novus Biologicals
    Average 90 stars, based on 2 article reviews
    monoclonal rat anti hcn4 - by Bioz Stars, 2026-05
    90/100 stars

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    FIGURE 2. Specific interaction between KCTD3 and HCN3 channels in mouse brain. A, identification of distinct HCN3-KCTD3 and HCN3-TRIP8b complexes. Protein lysates from brain tissue of wild type (WT) and HCN3 knock-out (KO) mice were immunoprecipitated with anti-HCN3, anti-KCTD3 or anti-TRIP8b antibodies. The precipitated samples were analyzed in Western blots using antibodies against HCN3, KCTD3, or TRPIP8b as indicated. B, KCTD3 does not bind to HCN1, HCN2, or <t>HCN4.</t> Brain lysates were immunoprecipitated with anti-KCTD3 antibody and immunoblotted with HCN channel-specific antibodies. For HCN1 and HCN2, analysis was performed in WT and KO mice, for HCN4 analysis was only performed in WT mice because HCN4 KO mice die in utero.
    Rat Monoclonal Hcn4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FIGURE 2. Specific interaction between KCTD3 and HCN3 channels in mouse brain. A, identification of distinct HCN3-KCTD3 and HCN3-TRIP8b complexes. Protein lysates from brain tissue of wild type (WT) and HCN3 knock-out (KO) mice were immunoprecipitated with anti-HCN3, anti-KCTD3 or anti-TRIP8b antibodies. The precipitated samples were analyzed in Western blots using antibodies against HCN3, KCTD3, or TRPIP8b as indicated. B, KCTD3 does not bind to HCN1, HCN2, or HCN4. Brain lysates were immunoprecipitated with anti-KCTD3 antibody and immunoblotted with HCN channel-specific antibodies. For HCN1 and HCN2, analysis was performed in WT and KO mice, for HCN4 analysis was only performed in WT mice because HCN4 KO mice die in utero.

    Journal: Journal of Biological Chemistry

    Article Title: Up-regulation of Hyperpolarization-activated Cyclic Nucleotide-gated Channel 3 (HCN3) by Specific Interaction with K+ Channel Tetramerization Domain-containing Protein 3 (KCTD3)

    doi: 10.1074/jbc.m112.434803

    Figure Lengend Snippet: FIGURE 2. Specific interaction between KCTD3 and HCN3 channels in mouse brain. A, identification of distinct HCN3-KCTD3 and HCN3-TRIP8b complexes. Protein lysates from brain tissue of wild type (WT) and HCN3 knock-out (KO) mice were immunoprecipitated with anti-HCN3, anti-KCTD3 or anti-TRIP8b antibodies. The precipitated samples were analyzed in Western blots using antibodies against HCN3, KCTD3, or TRPIP8b as indicated. B, KCTD3 does not bind to HCN1, HCN2, or HCN4. Brain lysates were immunoprecipitated with anti-KCTD3 antibody and immunoblotted with HCN channel-specific antibodies. For HCN1 and HCN2, analysis was performed in WT and KO mice, for HCN4 analysis was only performed in WT mice because HCN4 KO mice die in utero.

    Article Snippet: Antibodies—The following primary antibodies were used: mouse monoclonal -HCN1 (ab 84816, Abcam), rabbit polyclonal -HCN2 (43) (Western blot) or rabbit polyclonal -HCN2 (APC-030, Alomone) (immunofluorescence), rabbit polyclonal -HCN3 (34) (immunofluoresence), or mouse monoclonal -HCN3 (clone N141/28, 75–175, UC Davis/NIH NeuroMab) (immunoprecipitation and Western blot), rat monoclonal -HCN4 (sc-58622, Santa Cruz Biotechnology), rabbit polyclonal -KCTD3 (HPA015142, Sigma) (immunoprecipitation and Western blot), or goat polyclonal -KCTD3 (N-15, sc-164721, Santa Cruz Biotechnology) (immunofluoresence), mouse monoclonal -TRIP8b (clone N212/7, 75–243, UC Davis/NIH NeuroMab), mouse monoclonal -Myc (9B11, Cell Signaling/New England BioLabs Inc), mouse monoclonal -HA-tag (HA.11 clone 16B12, MMS-101P, Covance), mouse monoclonal -MAP2 (mAb HM2, Sigma), and mouse monoclonal -Na, K-ATPase subunit (clone 5, developed byD.M.

    Techniques: Knock-Out, Immunoprecipitation, Western Blot, In Utero