rabbit monoclonal anti p38 antibodies  (Cell Signaling Technology Inc)

Journal: The EMBO Journal

Article Title: A bacterial network of T3SS effectors counteracts host pro-inflammatory responses and cell death to promote infection

doi: 10.1038/s44318-025-00412-5

Figure Lengend Snippet: Reagents and tools table

Article Snippet: p-p38 (Thr180/Tyr182) rabbit monoclonal antibody , Cell Signaling Technology , #9215; RRID:AB_331762.

Techniques: Recombinant, Control, Sequencing, Cloning, Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Software, Imaging, Cell Analysis

Journal: Biomedicines

Article Title: Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.3390/biomedicines13020358

Figure Lengend Snippet: Western blot analysis of p-p38 and vimentin in SCC-25 and SCC25-E cells with and without irradiation. The original Western blot image of ( A ) p-p38 and ( B ) vimentin compared to the loading control GAPDH in control SCC-25 cells (1), in the mixed culture at passages 1 (2) and 3 (3), in irradiated SCC-25 cells (4), and in irradiated SCC25-E cells (p3) (5). Densitometry of ( C ) p-p38 and ( D ) vimentin normalized to the GAPDH loading control. The normalized optical density of the control SCC-25 cells was considered 100%, and the columns show the relative normalized optical density.

Article Snippet: IHC for phosphorylated p38 MAPK was performed using the rabbit monoclonal primary antibody against p-p38 MAPK (Cat. Nr. 4511S; 1:400), purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Irradiation, Control

Journal: Biomedicines

Article Title: Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.3390/biomedicines13020358

Figure Lengend Snippet: ( A ) Identification of the brown positive staining reaction of p-p38 in the nuclei of tumor cells in the original HNSCC cancer cell nest. ( B ) Cytoplasmic p-p38 antigen detection of single cells in the stromal compartment of the sectioned HNSCC tissue. Brown reactions without connections to cell nuclei are artifacts. The bottom left box of each image shows an overview of the whole scanned tissue section. The red box in each image shows the location of the presented area in the original tissue section.

Article Snippet: IHC for phosphorylated p38 MAPK was performed using the rabbit monoclonal primary antibody against p-p38 MAPK (Cat. Nr. 4511S; 1:400), purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Staining

Journal: Biomedicines

Article Title: Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.3390/biomedicines13020358

Figure Lengend Snippet: Western blot analysis of p-p38 and vimentin in SCC-25 and SCC25-E cells with and without irradiation. The original Western blot image of ( A ) p-p38 and ( B ) vimentin compared to the loading control GAPDH in control SCC-25 cells (1), in the mixed culture at passages 1 (2) and 3 (3), in irradiated SCC-25 cells (4), and in irradiated SCC25-E cells (p3) (5). Densitometry of ( C ) p-p38 and ( D ) vimentin normalized to the GAPDH loading control. The normalized optical density of the control SCC-25 cells was considered 100%, and the columns show the relative normalized optical density.

Article Snippet: Rabbit monoclonal primary antibodies against p-p38 MAPK (Cat. Nr. 4511S, 1:1000) and vimentin (SP-20, 1:100) were purchased from Cell Signaling Technology (Danvers, MA, USA) and Spring Biotech (Espoo, Finland).

Techniques: Western Blot, Irradiation, Control

Journal: Biomedicines

Article Title: Patient-Derived Cancer-Associated Fibroblasts Support the Colonization of Tumor Cells in Head and Neck Squamous Cell Carcinoma

doi: 10.3390/biomedicines13020358

Figure Lengend Snippet: ( A ) Identification of the brown positive staining reaction of p-p38 in the nuclei of tumor cells in the original HNSCC cancer cell nest. ( B ) Cytoplasmic p-p38 antigen detection of single cells in the stromal compartment of the sectioned HNSCC tissue. Brown reactions without connections to cell nuclei are artifacts. The bottom left box of each image shows an overview of the whole scanned tissue section. The red box in each image shows the location of the presented area in the original tissue section.

Article Snippet: Rabbit monoclonal primary antibodies against p-p38 MAPK (Cat. Nr. 4511S, 1:1000) and vimentin (SP-20, 1:100) were purchased from Cell Signaling Technology (Danvers, MA, USA) and Spring Biotech (Espoo, Finland).

Techniques: Staining