Review



monoclonal mouse anti human cytokeratin 8 18 19  (Millipore)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Millipore monoclonal mouse anti human cytokeratin 8 18 19
    ( A ) FHL2-silenced MDA-MB-231 cells were encapsulated within 3D alginate stiff for 3 days and exposed to 0.01 mM and higher doses of paclitaxel (0.01 to 0.5 mM) for 2 days. Percentage bars of viability (calcein + ) for vehicles and fold changes in viability and metabolic activity (PrestoBlue) with respect to untreated groups. * for statistical analysis with respect to the vehicle and # between groups. Student’s t test ( n = 3 hydrogels), */# P ≤ 0.05, **/## P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001. WT, wild type. ( B ) Intracardiac injection of GFP-tagged MDA-MB-231-1833 BoM cells in mice with Hematoxylin and Eosin (H&E) staining of the femur showing an osteolytic lesion (arrowhead). Confocal images of Ki67 and FHL2 localization of GFP-tagged MDA-MB-231-1833 BoM cells as clusters and single cells. Scale bars equal 1000 μm, 25 μm and 20 μm for H&E overview, cluster and single cell images, respectively. ( C ) Immunohistochemical image of FHL2 localization in a female patient (50 years old) with breast ductal carcinoma. Fraction of FHL2 localization in 34 patients with primary breast ductal carcinoma. Data obtained from The Human Protein Atlas . Scale bar equals 20 μm. ( D ) Confocal images of FHL2 localization in spheroids and single MDA-MB-231 cells, both kinds encapsulated within the same 3D Matrigel after 7 days of encapsulation and combined quantification of its nuc./cyto. ratio ( n = 19 cells) (blue = DAPI, green = F-Actin, red = FHL2). Scale bar equals 20 μm for spheroids and 10 μm for single cells images. ( E and F ) Confocal images of <t>cytokeratin</t> (epithelial marker) and FHL2 (in single cells and within clusters) of human early DCCs disseminated in the sentinel lymph node of an M0 patient. Scale bars equal 50 μm and 5 μm for the wide and zoomed images, respectively.
    Monoclonal Mouse Anti Human Cytokeratin 8 18 19, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti human cytokeratin 8 18 19/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti human cytokeratin 8 18 19 - by Bioz Stars, 2024-12
    86/100 stars

    Images

    1) Product Images from "Dormancy-inducing 3D engineered matrix uncovers mechanosensitive and drug-protective FHL2-p21 signaling axis"

    Article Title: Dormancy-inducing 3D engineered matrix uncovers mechanosensitive and drug-protective FHL2-p21 signaling axis

    Journal: Science Advances

    doi: 10.1126/sciadv.adr3997

    ( A ) FHL2-silenced MDA-MB-231 cells were encapsulated within 3D alginate stiff for 3 days and exposed to 0.01 mM and higher doses of paclitaxel (0.01 to 0.5 mM) for 2 days. Percentage bars of viability (calcein + ) for vehicles and fold changes in viability and metabolic activity (PrestoBlue) with respect to untreated groups. * for statistical analysis with respect to the vehicle and # between groups. Student’s t test ( n = 3 hydrogels), */# P ≤ 0.05, **/## P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001. WT, wild type. ( B ) Intracardiac injection of GFP-tagged MDA-MB-231-1833 BoM cells in mice with Hematoxylin and Eosin (H&E) staining of the femur showing an osteolytic lesion (arrowhead). Confocal images of Ki67 and FHL2 localization of GFP-tagged MDA-MB-231-1833 BoM cells as clusters and single cells. Scale bars equal 1000 μm, 25 μm and 20 μm for H&E overview, cluster and single cell images, respectively. ( C ) Immunohistochemical image of FHL2 localization in a female patient (50 years old) with breast ductal carcinoma. Fraction of FHL2 localization in 34 patients with primary breast ductal carcinoma. Data obtained from The Human Protein Atlas . Scale bar equals 20 μm. ( D ) Confocal images of FHL2 localization in spheroids and single MDA-MB-231 cells, both kinds encapsulated within the same 3D Matrigel after 7 days of encapsulation and combined quantification of its nuc./cyto. ratio ( n = 19 cells) (blue = DAPI, green = F-Actin, red = FHL2). Scale bar equals 20 μm for spheroids and 10 μm for single cells images. ( E and F ) Confocal images of cytokeratin (epithelial marker) and FHL2 (in single cells and within clusters) of human early DCCs disseminated in the sentinel lymph node of an M0 patient. Scale bars equal 50 μm and 5 μm for the wide and zoomed images, respectively.
    Figure Legend Snippet: ( A ) FHL2-silenced MDA-MB-231 cells were encapsulated within 3D alginate stiff for 3 days and exposed to 0.01 mM and higher doses of paclitaxel (0.01 to 0.5 mM) for 2 days. Percentage bars of viability (calcein + ) for vehicles and fold changes in viability and metabolic activity (PrestoBlue) with respect to untreated groups. * for statistical analysis with respect to the vehicle and # between groups. Student’s t test ( n = 3 hydrogels), */# P ≤ 0.05, **/## P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001. WT, wild type. ( B ) Intracardiac injection of GFP-tagged MDA-MB-231-1833 BoM cells in mice with Hematoxylin and Eosin (H&E) staining of the femur showing an osteolytic lesion (arrowhead). Confocal images of Ki67 and FHL2 localization of GFP-tagged MDA-MB-231-1833 BoM cells as clusters and single cells. Scale bars equal 1000 μm, 25 μm and 20 μm for H&E overview, cluster and single cell images, respectively. ( C ) Immunohistochemical image of FHL2 localization in a female patient (50 years old) with breast ductal carcinoma. Fraction of FHL2 localization in 34 patients with primary breast ductal carcinoma. Data obtained from The Human Protein Atlas . Scale bar equals 20 μm. ( D ) Confocal images of FHL2 localization in spheroids and single MDA-MB-231 cells, both kinds encapsulated within the same 3D Matrigel after 7 days of encapsulation and combined quantification of its nuc./cyto. ratio ( n = 19 cells) (blue = DAPI, green = F-Actin, red = FHL2). Scale bar equals 20 μm for spheroids and 10 μm for single cells images. ( E and F ) Confocal images of cytokeratin (epithelial marker) and FHL2 (in single cells and within clusters) of human early DCCs disseminated in the sentinel lymph node of an M0 patient. Scale bars equal 50 μm and 5 μm for the wide and zoomed images, respectively.

    Techniques Used: Activity Assay, Injection, Staining, Immunohistochemical staining, Encapsulation, Marker



    Similar Products

    monoclonal mouse anti human cytokeratin 8 18 19  (Millipore)
    86
    Millipore monoclonal mouse anti human cytokeratin 8 18 19
    ( A ) FHL2-silenced MDA-MB-231 cells were encapsulated within 3D alginate stiff for 3 days and exposed to 0.01 mM and higher doses of paclitaxel (0.01 to 0.5 mM) for 2 days. Percentage bars of viability (calcein + ) for vehicles and fold changes in viability and metabolic activity (PrestoBlue) with respect to untreated groups. * for statistical analysis with respect to the vehicle and # between groups. Student’s t test ( n = 3 hydrogels), */# P ≤ 0.05, **/## P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001. WT, wild type. ( B ) Intracardiac injection of GFP-tagged MDA-MB-231-1833 BoM cells in mice with Hematoxylin and Eosin (H&E) staining of the femur showing an osteolytic lesion (arrowhead). Confocal images of Ki67 and FHL2 localization of GFP-tagged MDA-MB-231-1833 BoM cells as clusters and single cells. Scale bars equal 1000 μm, 25 μm and 20 μm for H&E overview, cluster and single cell images, respectively. ( C ) Immunohistochemical image of FHL2 localization in a female patient (50 years old) with breast ductal carcinoma. Fraction of FHL2 localization in 34 patients with primary breast ductal carcinoma. Data obtained from The Human Protein Atlas . Scale bar equals 20 μm. ( D ) Confocal images of FHL2 localization in spheroids and single MDA-MB-231 cells, both kinds encapsulated within the same 3D Matrigel after 7 days of encapsulation and combined quantification of its nuc./cyto. ratio ( n = 19 cells) (blue = DAPI, green = F-Actin, red = FHL2). Scale bar equals 20 μm for spheroids and 10 μm for single cells images. ( E and F ) Confocal images of <t>cytokeratin</t> (epithelial marker) and FHL2 (in single cells and within clusters) of human early DCCs disseminated in the sentinel lymph node of an M0 patient. Scale bars equal 50 μm and 5 μm for the wide and zoomed images, respectively.
    Monoclonal Mouse Anti Human Cytokeratin 8 18 19, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti human cytokeratin 8 18 19/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti human cytokeratin 8 18 19 - by Bioz Stars, 2024-12
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) FHL2-silenced MDA-MB-231 cells were encapsulated within 3D alginate stiff for 3 days and exposed to 0.01 mM and higher doses of paclitaxel (0.01 to 0.5 mM) for 2 days. Percentage bars of viability (calcein + ) for vehicles and fold changes in viability and metabolic activity (PrestoBlue) with respect to untreated groups. * for statistical analysis with respect to the vehicle and # between groups. Student’s t test ( n = 3 hydrogels), */# P ≤ 0.05, **/## P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001. WT, wild type. ( B ) Intracardiac injection of GFP-tagged MDA-MB-231-1833 BoM cells in mice with Hematoxylin and Eosin (H&E) staining of the femur showing an osteolytic lesion (arrowhead). Confocal images of Ki67 and FHL2 localization of GFP-tagged MDA-MB-231-1833 BoM cells as clusters and single cells. Scale bars equal 1000 μm, 25 μm and 20 μm for H&E overview, cluster and single cell images, respectively. ( C ) Immunohistochemical image of FHL2 localization in a female patient (50 years old) with breast ductal carcinoma. Fraction of FHL2 localization in 34 patients with primary breast ductal carcinoma. Data obtained from The Human Protein Atlas . Scale bar equals 20 μm. ( D ) Confocal images of FHL2 localization in spheroids and single MDA-MB-231 cells, both kinds encapsulated within the same 3D Matrigel after 7 days of encapsulation and combined quantification of its nuc./cyto. ratio ( n = 19 cells) (blue = DAPI, green = F-Actin, red = FHL2). Scale bar equals 20 μm for spheroids and 10 μm for single cells images. ( E and F ) Confocal images of cytokeratin (epithelial marker) and FHL2 (in single cells and within clusters) of human early DCCs disseminated in the sentinel lymph node of an M0 patient. Scale bars equal 50 μm and 5 μm for the wide and zoomed images, respectively.

    Journal: Science Advances

    Article Title: Dormancy-inducing 3D engineered matrix uncovers mechanosensitive and drug-protective FHL2-p21 signaling axis

    doi: 10.1126/sciadv.adr3997

    Figure Lengend Snippet: ( A ) FHL2-silenced MDA-MB-231 cells were encapsulated within 3D alginate stiff for 3 days and exposed to 0.01 mM and higher doses of paclitaxel (0.01 to 0.5 mM) for 2 days. Percentage bars of viability (calcein + ) for vehicles and fold changes in viability and metabolic activity (PrestoBlue) with respect to untreated groups. * for statistical analysis with respect to the vehicle and # between groups. Student’s t test ( n = 3 hydrogels), */# P ≤ 0.05, **/## P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001. WT, wild type. ( B ) Intracardiac injection of GFP-tagged MDA-MB-231-1833 BoM cells in mice with Hematoxylin and Eosin (H&E) staining of the femur showing an osteolytic lesion (arrowhead). Confocal images of Ki67 and FHL2 localization of GFP-tagged MDA-MB-231-1833 BoM cells as clusters and single cells. Scale bars equal 1000 μm, 25 μm and 20 μm for H&E overview, cluster and single cell images, respectively. ( C ) Immunohistochemical image of FHL2 localization in a female patient (50 years old) with breast ductal carcinoma. Fraction of FHL2 localization in 34 patients with primary breast ductal carcinoma. Data obtained from The Human Protein Atlas . Scale bar equals 20 μm. ( D ) Confocal images of FHL2 localization in spheroids and single MDA-MB-231 cells, both kinds encapsulated within the same 3D Matrigel after 7 days of encapsulation and combined quantification of its nuc./cyto. ratio ( n = 19 cells) (blue = DAPI, green = F-Actin, red = FHL2). Scale bar equals 20 μm for spheroids and 10 μm for single cells images. ( E and F ) Confocal images of cytokeratin (epithelial marker) and FHL2 (in single cells and within clusters) of human early DCCs disseminated in the sentinel lymph node of an M0 patient. Scale bars equal 50 μm and 5 μm for the wide and zoomed images, respectively.

    Article Snippet: Primary antibody incubation with monoclonal mouse anti-human cytokeratin 8/18/19 (2 μg/ml, clone A45-B/B3, AS Diagnostik) and polyclonal rabbit anti-human FHL2 (2 μg/ml, HPA006028, Sigma-Aldrich/Prestige Antibodies) was performed at 4°C overnight in PBS/10% AB serum.

    Techniques: Activity Assay, Injection, Staining, Immunohistochemical staining, Encapsulation, Marker