Journal: Revista Brasileira de Cirurgia Cardiovascular : órgão oficial da Sociedade Brasileira de Cirurgia Cardiovascular
Article Title: Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
doi: 10.5935/1678-9741.20150004
Figure Lengend Snippet: Immunostaining of sections of stenotic valve from a patient with rheumatic fever. Top left. ET-1 staining associated with fibrotic regions and neovascularization. Top middle. ETA receptor staining of fibrotic region and neovascularization. Top right. H + E-stained section. Lower left. No ET-3 immunostaining. Lower middle. ETB receptor immunostaining of fibrotic region and neovascularization. Lower right. Negative control. ET=endothelin; ETA=endothelin receptor A; ETB=endothelin receptor B
Article Snippet: Antibodies and their dilution: Mouse anti-human monoclonal antibody against endothelial cells (CD31, clone JC70A, 1:100, DakoCytomation, Denmark); rabbit anti-human monoclonal antibody against ET-1 and ET-3 (1:250, R & D Labs), rabbit anti-human monoclonal antibodies against ETA and ETB receptors (both 1:200, Alomone Labs, Israel) and AS02/Thy-1 for fibroblasts (1:200, DakoCytomation, Denmark).
Techniques: Immunostaining, Staining, Negative Control