monoclonal antibody against crp  (Medix Biochemica)

Journal: Journal of Clinical Medicine

Article Title: C-Reactive Protein (CRP) Blocks the Desensitization of Agonistic Stimulated G Protein Coupled Receptors (GPCRs) in Neonatal Rat Cardiomyocytes

doi: 10.3390/jcm11041058

Figure Lengend Snippet: CRP blocks the desensitization of stimulated neonatal rat cardiomyocytes with ISO. Difference of cardiomyocyte beating rates [Δ Beating rate/15 s] modulated by adrenoceptor agonist isoprenaline (ISO) with or without CRP co-incubation. ( A ) Mean of 3–7 independent experiments ± standard deviation is depicted. Arrows indicate stimulation with respective agonists. Grey area indicates washing step. After the washing step, the agonist was added again but no CRP. To test if CRP itself influences the beating rate, cells were incubated with CRP and no change could be observed after 5 min (grey circle). Significance of difference between CRP incubated and control group and mAb group at 120 min and 130 min was calculated with two-way ANOVA with Bonferroni post-hoc test. ( B ) Same experimental setup as depicted in ( A ), but only the beating rate at the last time point (130 min) is shown as mean of 3–5 independent experiments ± standard deviation or as single value (20–2 µg/mL CRP). Significance of difference between 40 µg/mL CRP and control (only ISO) was calculated with student’s t -test. ** p < 0.01. *** p < 0.001. ns: not significant.

Article Snippet: The murine monoclonal antibody (0.64 mg/mL) against CRP was provided by Biometec Inc. (Dr. S. Witt, Biometec GmbH, Greifswald, Germany) and generated by Dr. B. Micheel and Dr. W. Schroedl.

Techniques: Incubation, Standard Deviation, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Anti-Inflammatory and Antioxidant Effects of Acetyl-L-Carnitine on Atherosclerotic Rats

doi: 10.12659/MSM.920250

Figure Lengend Snippet: Effects of ALCAR on the mRNA expressions of CRP, TNF-α, IL-1β, and iNOS in aorta and heart tissues of AS rats. ( A ) The relative mRNA expression of CRP, TNF-α, IL-1β, and iNOS in the aorta tissue among the control, AS, ALCAR, and AS+ALCAR groups. ( B ) The relative mRNA expression of CRP, TNF-α, IL-1β, and iNOS in the heart tissue among the control, AS, ALCAR, and AS+ALCAR groups. Compared with the control group, * p<0.05 and ** P <0.01; Compared with the AS group, ## P <0.01.

Article Snippet: After blocking with 5% fat-free milk powder for 2 h at room temperature, the membranes were incubated overnight at 4°C with primary antibodies against CRP (#14316, dilution 1: 1000), IL-1β (#31202, dilution 1: 1000), TNF-α (#11948, dilution 1: 1000), iNOS (#2982, dilution 1: 1000) from Cell Signaling Technology, Inc., and β-actin (sc-130656, dilution 1: 1000) from Santa Cruz Biotechnology.

Techniques: Expressing, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Anti-Inflammatory and Antioxidant Effects of Acetyl-L-Carnitine on Atherosclerotic Rats

doi: 10.12659/MSM.920250

Figure Lengend Snippet: Effects of ALCAR on the protein expressions of CRP, TNF-α, IL-1β, and iNOS in aorta and heart tissues of AS rats. ( A ) The relative protein expression of CRP, TNF-α, IL-1β and iNOS in the aorta tissue among the control, AS, ALCAR, and AS+ALCAR groups. ( B ) The relative protein expression of CRP, TNF-α, IL-1β, and iNOS in the heart tissue among the control, AS, ALCAR, and AS+ALCAR groups. Compared with the control group, * p<0.05 and ** P <0.01; Compared with the AS group, ## P <0.01.

Article Snippet: After blocking with 5% fat-free milk powder for 2 h at room temperature, the membranes were incubated overnight at 4°C with primary antibodies against CRP (#14316, dilution 1: 1000), IL-1β (#31202, dilution 1: 1000), TNF-α (#11948, dilution 1: 1000), iNOS (#2982, dilution 1: 1000) from Cell Signaling Technology, Inc., and β-actin (sc-130656, dilution 1: 1000) from Santa Cruz Biotechnology.

Techniques: Expressing, Control