Structured Review

Merck & Co monensin
IR increases cellular methylase activity through its coupling to a SAM-producing metabolic pathway. ( A ) Effects of IR, the respiratory chain inhibitor AA and <t>monensin</t> (10 µM) on quinacrine-stained breast cancer cells. Cells were loaded with 10 µM quinacrine. IR led to the accumulation of quinacrine fluorescence in breast cancer cells, whereas AA and monensin led to the loss of quinacrine fluorescence. w/o, without treatment. Scale bars refer to 10 µm. ( B ) The histogram represents the effects of IR (10 Gy, 12 h) and/or AA on ATP levels in breast cancer cells. * P
Monensin, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monensin/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
monensin - by Bioz Stars, 2021-03
86/100 stars

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1) Product Images from "PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation"

Article Title: PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation

Journal: Scientific Reports

doi: 10.1038/s41598-020-70289-3

IR increases cellular methylase activity through its coupling to a SAM-producing metabolic pathway. ( A ) Effects of IR, the respiratory chain inhibitor AA and monensin (10 µM) on quinacrine-stained breast cancer cells. Cells were loaded with 10 µM quinacrine. IR led to the accumulation of quinacrine fluorescence in breast cancer cells, whereas AA and monensin led to the loss of quinacrine fluorescence. w/o, without treatment. Scale bars refer to 10 µm. ( B ) The histogram represents the effects of IR (10 Gy, 12 h) and/or AA on ATP levels in breast cancer cells. * P
Figure Legend Snippet: IR increases cellular methylase activity through its coupling to a SAM-producing metabolic pathway. ( A ) Effects of IR, the respiratory chain inhibitor AA and monensin (10 µM) on quinacrine-stained breast cancer cells. Cells were loaded with 10 µM quinacrine. IR led to the accumulation of quinacrine fluorescence in breast cancer cells, whereas AA and monensin led to the loss of quinacrine fluorescence. w/o, without treatment. Scale bars refer to 10 µm. ( B ) The histogram represents the effects of IR (10 Gy, 12 h) and/or AA on ATP levels in breast cancer cells. * P

Techniques Used: Activity Assay, Staining, Fluorescence

Related Articles

Staining:

Article Title: Breakdown of adaptive immunotolerance induces hepatocellular carcinoma in HBsAg-tg mice
Article Snippet: FITC-PD-1, APC-CD226; Alexa Fluor 647-anti-Foxp3, Alexa Fluor 660(AF 660) anti-TIGIT (clone: GIGD7), and AF 660-Ki67 were purchased from eBioscience (San Diego, CA, USA). .. For IFN-γ staining, cells were stimulated with 30 ng/ml PMA (CalBioChem), 1 μg/ml ionomycin (Sigma), and Monensin (10 μg/ml; Merck) for 4 h at 37 °C. .. For CD107a staining, anti-CD107a mAb was added as well.

other:

Article Title: PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation
Article Snippet: Reagents and antibodiesAA, AdOx, quinacrine, monensin, MG132, and AZA were obtained from Merck (Madrid, Spain).

Cell Stimulation:

Article Title: Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
Article Snippet: Results were expressed as chemotaxis index. .. T-cell stimulation, cytokine production and cytotoxic assay Purified CD45+ TIL were co-cultured for 4 h with B16F10 tumour cells, pulsed with Trp2 (1 µg) and gp100 (1 µg) peptides, in the presence of Brefeldin A (Invitrogen 00-4506-51, 1/1000), monensin (Merck M5273, 2 µm ) and anti-CD107a mAb (clone 1D4B, Biolegend 121619, 1/50). .. TIL were stained with mAb specific for surface proteins prior to fixation and permeabilization.

Purification:

Article Title: Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
Article Snippet: Results were expressed as chemotaxis index. .. T-cell stimulation, cytokine production and cytotoxic assay Purified CD45+ TIL were co-cultured for 4 h with B16F10 tumour cells, pulsed with Trp2 (1 µg) and gp100 (1 µg) peptides, in the presence of Brefeldin A (Invitrogen 00-4506-51, 1/1000), monensin (Merck M5273, 2 µm ) and anti-CD107a mAb (clone 1D4B, Biolegend 121619, 1/50). .. TIL were stained with mAb specific for surface proteins prior to fixation and permeabilization.

Isolation:

Article Title: Tumor-Targeting Anti-CD20 Antibodies Mediate In Vitro Expansion of Memory Natural Killer Cells: Impact of CD16 Affinity Ligation Conditions and In Vivo Priming
Article Snippet: The ratio between the mean fluorescence intensity (MFI) of MEM-154 and 3G8 allows to classify the following three different phenotypes: F/F (ratio < 0.04), V/V (ratio > 0.62), and V/F (ratio between 0.15 and 0.48). .. Freshly isolated PBMCs and their in vitro cultured counterparts were stimulated with Raji cells (2:1), opsonized or not with the minimum saturating dose of rituximab (1 μg/1 × 106 ) or obinutuzumab (0.1 μg/1 × 106 ), or with K562 targets, for 6 h at 37°C in the presence of PE-conjugated anti-CD107a mAb (clone: H4A3, cat #: 555801; BD Biosciences) and 50 µM Monensin (Golgi-stop; cat #: M5273; Merck). .. After the first hour, 10 µg/ml Brefeldin A (cat #: B7651; Merck) was added.

In Vitro:

Article Title: Tumor-Targeting Anti-CD20 Antibodies Mediate In Vitro Expansion of Memory Natural Killer Cells: Impact of CD16 Affinity Ligation Conditions and In Vivo Priming
Article Snippet: The ratio between the mean fluorescence intensity (MFI) of MEM-154 and 3G8 allows to classify the following three different phenotypes: F/F (ratio < 0.04), V/V (ratio > 0.62), and V/F (ratio between 0.15 and 0.48). .. Freshly isolated PBMCs and their in vitro cultured counterparts were stimulated with Raji cells (2:1), opsonized or not with the minimum saturating dose of rituximab (1 μg/1 × 106 ) or obinutuzumab (0.1 μg/1 × 106 ), or with K562 targets, for 6 h at 37°C in the presence of PE-conjugated anti-CD107a mAb (clone: H4A3, cat #: 555801; BD Biosciences) and 50 µM Monensin (Golgi-stop; cat #: M5273; Merck). .. After the first hour, 10 µg/ml Brefeldin A (cat #: B7651; Merck) was added.

Cell Culture:

Article Title: Tumor-Targeting Anti-CD20 Antibodies Mediate In Vitro Expansion of Memory Natural Killer Cells: Impact of CD16 Affinity Ligation Conditions and In Vivo Priming
Article Snippet: The ratio between the mean fluorescence intensity (MFI) of MEM-154 and 3G8 allows to classify the following three different phenotypes: F/F (ratio < 0.04), V/V (ratio > 0.62), and V/F (ratio between 0.15 and 0.48). .. Freshly isolated PBMCs and their in vitro cultured counterparts were stimulated with Raji cells (2:1), opsonized or not with the minimum saturating dose of rituximab (1 μg/1 × 106 ) or obinutuzumab (0.1 μg/1 × 106 ), or with K562 targets, for 6 h at 37°C in the presence of PE-conjugated anti-CD107a mAb (clone: H4A3, cat #: 555801; BD Biosciences) and 50 µM Monensin (Golgi-stop; cat #: M5273; Merck). .. After the first hour, 10 µg/ml Brefeldin A (cat #: B7651; Merck) was added.

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    Merck & Co monensin
    Monensin, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monensin/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monensin - by Bioz Stars, 2021-03
    86/100 stars
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