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FUJIFILM monensin
Monensin, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monensin/product/FUJIFILM
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
monensin - by Bioz Stars, 2021-03
99/100 stars

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other:

Article Title: Cytosolic chloride ion is a key factor in lysosomal acidification and function of autophagy in human gastric cancer cell
Article Snippet: Nigericin, tributyltin chloride and monensin were purchased from Wako Pure Chemical Industries (Osaka, Japan).

Fluorescence:

Article Title: Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA
Article Snippet: .. To evaluate pH-dependent change of EYFP-er fluorescence , we stimulated HeLa cells expressing EYFP-er in a PSS (adjusted to pH 6.8) containing monensin (10 μM, Wako) and nigericin (10 μM, Wako). ..

Expressing:

Article Title: Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA
Article Snippet: .. To evaluate pH-dependent change of EYFP-er fluorescence , we stimulated HeLa cells expressing EYFP-er in a PSS (adjusted to pH 6.8) containing monensin (10 μM, Wako) and nigericin (10 μM, Wako). ..

Isolation:

Article Title: Analysis of in Vivo Role of ?-Fodrin Autoantigen in Primary Sj?gren's Syndrome
Article Snippet: During the last 8 hours of the 72-hour culture period, 1 μCi of [3 H]thymidine was added per well, and the incorporated radioactivity was determined using an automated β liquid scintillation counter. .. For analysis of intracellular cytokines, monensin (Wako Pure Chemical, Osaka, Japan) was added at 2 μmol/L to isolated PBMCs (106 /ml), and 2 hours later the cells were collected, labeled with anti-CD4-PE (BD PharMingen), fixed with 4% paraformaldehyde for 10 minutes at 4°C, and then permeabilized with 0.1% saponin in PBS at room temperature for 10 minutes. .. Cells were incubated with anti-interleukin (IL)-2-fluorescein isothiocyanate (FITC) (8 μg/ml; BD PharMingen), anti-IL-4-FITC (5 μg/ml; BD PharMingen), and anti-interferon (IFN)-γ-FITC (1 μg/ml; BD PharMingen), respectively, and analyzed on a EPICS flow cytometer (Beckman Coulter, Miami, FL).

Labeling:

Article Title: Analysis of in Vivo Role of ?-Fodrin Autoantigen in Primary Sj?gren's Syndrome
Article Snippet: During the last 8 hours of the 72-hour culture period, 1 μCi of [3 H]thymidine was added per well, and the incorporated radioactivity was determined using an automated β liquid scintillation counter. .. For analysis of intracellular cytokines, monensin (Wako Pure Chemical, Osaka, Japan) was added at 2 μmol/L to isolated PBMCs (106 /ml), and 2 hours later the cells were collected, labeled with anti-CD4-PE (BD PharMingen), fixed with 4% paraformaldehyde for 10 minutes at 4°C, and then permeabilized with 0.1% saponin in PBS at room temperature for 10 minutes. .. Cells were incubated with anti-interleukin (IL)-2-fluorescein isothiocyanate (FITC) (8 μg/ml; BD PharMingen), anti-IL-4-FITC (5 μg/ml; BD PharMingen), and anti-interferon (IFN)-γ-FITC (1 μg/ml; BD PharMingen), respectively, and analyzed on a EPICS flow cytometer (Beckman Coulter, Miami, FL).

Staining:

Article Title: Human amnion-derived mesenchymal stem cells attenuate xenogeneic graft-versus-host disease by preventing T cell activation and proliferation
Article Snippet: .. For intracellular staining, 2 μM monensin (Fujifilm) was added for 4 h at 37 °C and cells permeabilized using the FOXP3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Data were analyzed using FlowJo software (FlowJo, OR, USA).

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    FUJIFILM monensin
    Monensin, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monensin/product/FUJIFILM
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monensin - by Bioz Stars, 2021-03
    99/100 stars
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