monarch nucleic acid purification kit  (New England Biolabs)


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    Name:
    Monarch DNA Gel Extraction Kit
    Description:
    Monarch DNA Gel Extraction Kit 250 preps
    Catalog Number:
    t1020l
    Price:
    466
    Size:
    250 preps
    Category:
    DNA Fragment Purification from Gels
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    Structured Review

    New England Biolabs monarch nucleic acid purification kit
    Monarch DNA Gel Extraction Kit
    Monarch DNA Gel Extraction Kit 250 preps
    https://www.bioz.com/result/monarch nucleic acid purification kit/product/New England Biolabs
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    monarch nucleic acid purification kit - by Bioz Stars, 2020-07
    99/100 stars

    Related Products / Commonly Used Together

    amplicons
    bp gel fragments

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    Related Articles

    DNA Extraction:

    Article Title: Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells
    Article Snippet: .. The DNA was electrophoresed in a 3% MetaPhor agarose (FMC Bioproducts, Rockland, ME) gel, excised, purified (Monarch DNA extraction kit, New England Biolabs), and sequenced (BigDye Terminator® version 3.1 cycle sequencing kit, Thermo Fisher Scientific). .. The following reporter gene constructs were generated using the pGL3 luciferase vector (Promega) with the SV40 promoter element removed: (i) empty vector control (CNT), (ii) −1990 to +50 bp of the CAST promoter linked to the luciferase gene, with +1 denoting the first nucleotide of exon 1 (designated CP for canonical promoter region), and (iii) −4026 to +20 bp relative to exon 4, with +1 denoting the first nucleotide exon 4 (designated ALT for NFI-bound alternative promoter).

    Amplification:

    Article Title: Antha-guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries
    Article Snippet: .. Purify the expected PCR amplicon from agarose gels stained with SYBR safe (Life Technologies) using Monarch DNA Gel Extraction kits (NEB) following manufacturer’s recommended protocol. .. Quantify the recovered DNA using a Qubit fluorometer (Thermo Fisher Scientific).

    Agarose Gel Electrophoresis:

    Article Title: A novel allele of ASY3 promotes meiotic stability in autotetraploid Arabidopsis lyrata
    Article Snippet: .. PCR products were electrophoresed on a 2% (w/v) agarose gel and the lower band (∼125 bp) corresponding to a partial ASY3 DEL allele was excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs). .. Purified PCR products were cloned into pCR™ 4-TOPO® vector using a TOPO™ TA Cloning™ for Sequencing Kit (ThermoFisher Scientific) following manufacturer’s instructions.

    Purification:

    Article Title: Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells
    Article Snippet: .. The DNA was electrophoresed in a 3% MetaPhor agarose (FMC Bioproducts, Rockland, ME) gel, excised, purified (Monarch DNA extraction kit, New England Biolabs), and sequenced (BigDye Terminator® version 3.1 cycle sequencing kit, Thermo Fisher Scientific). .. The following reporter gene constructs were generated using the pGL3 luciferase vector (Promega) with the SV40 promoter element removed: (i) empty vector control (CNT), (ii) −1990 to +50 bp of the CAST promoter linked to the luciferase gene, with +1 denoting the first nucleotide of exon 1 (designated CP for canonical promoter region), and (iii) −4026 to +20 bp relative to exon 4, with +1 denoting the first nucleotide exon 4 (designated ALT for NFI-bound alternative promoter).

    Article Title: A novel allele of ASY3 promotes meiotic stability in autotetraploid Arabidopsis lyrata
    Article Snippet: .. PCR products were electrophoresed on a 2% (w/v) agarose gel and the lower band (∼125 bp) corresponding to a partial ASY3 DEL allele was excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs). .. Purified PCR products were cloned into pCR™ 4-TOPO® vector using a TOPO™ TA Cloning™ for Sequencing Kit (ThermoFisher Scientific) following manufacturer’s instructions.

    Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior
    Article Snippet: .. All PCR products and plasmids were purified using Monarch DNA Gel Extraction Kit (NEB, United States) and MiniBEST Plasmid Purification Kit (Takara, Japan), respectively. ..

    Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
    Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Monarch DNA Gel Extraction Kit (New England Biolabs, Frankfurt, Germany) as recommended by the manufacturer. .. Mice were sacrificed by cervical dislocation and the lungs were removed after ligation of the trachea to avoid alveolar collapse as described .

    Sequencing:

    Article Title: Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells
    Article Snippet: .. The DNA was electrophoresed in a 3% MetaPhor agarose (FMC Bioproducts, Rockland, ME) gel, excised, purified (Monarch DNA extraction kit, New England Biolabs), and sequenced (BigDye Terminator® version 3.1 cycle sequencing kit, Thermo Fisher Scientific). .. The following reporter gene constructs were generated using the pGL3 luciferase vector (Promega) with the SV40 promoter element removed: (i) empty vector control (CNT), (ii) −1990 to +50 bp of the CAST promoter linked to the luciferase gene, with +1 denoting the first nucleotide of exon 1 (designated CP for canonical promoter region), and (iii) −4026 to +20 bp relative to exon 4, with +1 denoting the first nucleotide exon 4 (designated ALT for NFI-bound alternative promoter).

    Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
    Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Monarch DNA Gel Extraction Kit (New England Biolabs, Frankfurt, Germany) as recommended by the manufacturer. .. Mice were sacrificed by cervical dislocation and the lungs were removed after ligation of the trachea to avoid alveolar collapse as described .

    Gel Extraction:

    Article Title: A novel allele of ASY3 promotes meiotic stability in autotetraploid Arabidopsis lyrata
    Article Snippet: .. PCR products were electrophoresed on a 2% (w/v) agarose gel and the lower band (∼125 bp) corresponding to a partial ASY3 DEL allele was excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs). .. Purified PCR products were cloned into pCR™ 4-TOPO® vector using a TOPO™ TA Cloning™ for Sequencing Kit (ThermoFisher Scientific) following manufacturer’s instructions.

    Article Title: Evolution of a General RNA-Cleaving FANA Enzyme
    Article Snippet: .. ThermoPol buffer, Taq DNA polymerase, G. stearothermophilus Bst DNA polymerase, LF, and its variants Bst 2.0 DNA polymerase (2.0), and Bst 3.0 DNA polymerase (3.0), DH5α competent cells, and Monarch DNA gel extraction kits were purchased from New England Biolabs (Ipswich, MA). .. 3′–5′ exonuclease-deficient (exo−) archaeal polymerases isolated from Thermococcus sp. 9°N (9°N), Pyrococcus sp. deep vent (DV), T. gorgonarius (Tgo), T. kodakarensis (Kod), and G. stearothermophilus Bst DNA polymerase, LF* were expressed and purified from E. coli of the XL1-Blue strain from Agilent Technologies (Santa Clara, CA) as previously described .

    Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior
    Article Snippet: .. All PCR products and plasmids were purified using Monarch DNA Gel Extraction Kit (NEB, United States) and MiniBEST Plasmid Purification Kit (Takara, Japan), respectively. ..

    Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
    Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Monarch DNA Gel Extraction Kit (New England Biolabs, Frankfurt, Germany) as recommended by the manufacturer. .. Mice were sacrificed by cervical dislocation and the lungs were removed after ligation of the trachea to avoid alveolar collapse as described .

    Article Title: Antha-guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries
    Article Snippet: .. Purify the expected PCR amplicon from agarose gels stained with SYBR safe (Life Technologies) using Monarch DNA Gel Extraction kits (NEB) following manufacturer’s recommended protocol. .. Quantify the recovered DNA using a Qubit fluorometer (Thermo Fisher Scientific).

    Article Title: Evolution of a General RNA-Cleaving FANA Enzyme
    Article Snippet: .. ThermoPol buffer, Taq DNA polymerase, G. stearothermophilus Bst DNA polymerase, LF, and its variants Bst 2.0 DNA polymerase (2.0), and Bst 3.0 DNA polymerase (3.0), DH5α competent cells, and Monarch DNA gel extraction kits were purchased from New England Biolabs (Ipswich, MA). .. 3′–5′ exonuclease-deficient (exo−) archaeal polymerases isolated from Thermococcus sp. 9°N (9°N), Pyrococcus sp. deep vent (DV), T. gorgonarius (Tgo), T. kodakarensis (Kod), and G. stearothermophilus Bst DNA polymerase, LF* were expressed and purified from E. coli of the XL1-Blue strain from Agilent Technologies (Santa Clara, CA) as previously described .

    Polymerase Chain Reaction:

    Article Title: A novel allele of ASY3 promotes meiotic stability in autotetraploid Arabidopsis lyrata
    Article Snippet: .. PCR products were electrophoresed on a 2% (w/v) agarose gel and the lower band (∼125 bp) corresponding to a partial ASY3 DEL allele was excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs). .. Purified PCR products were cloned into pCR™ 4-TOPO® vector using a TOPO™ TA Cloning™ for Sequencing Kit (ThermoFisher Scientific) following manufacturer’s instructions.

    Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior
    Article Snippet: .. All PCR products and plasmids were purified using Monarch DNA Gel Extraction Kit (NEB, United States) and MiniBEST Plasmid Purification Kit (Takara, Japan), respectively. ..

    Article Title: Bordetella pseudohinzii targets cilia and impairs tracheal cilia-driven transport in naturally acquired infection in mice
    Article Snippet: .. For subsequent sequencing, PCR-products were purified by the use of the Monarch DNA Gel Extraction Kit (New England Biolabs, Frankfurt, Germany) as recommended by the manufacturer. .. Mice were sacrificed by cervical dislocation and the lungs were removed after ligation of the trachea to avoid alveolar collapse as described .

    Article Title: Antha-guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries
    Article Snippet: .. Purify the expected PCR amplicon from agarose gels stained with SYBR safe (Life Technologies) using Monarch DNA Gel Extraction kits (NEB) following manufacturer’s recommended protocol. .. Quantify the recovered DNA using a Qubit fluorometer (Thermo Fisher Scientific).

    Staining:

    Article Title: Antha-guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries
    Article Snippet: .. Purify the expected PCR amplicon from agarose gels stained with SYBR safe (Life Technologies) using Monarch DNA Gel Extraction kits (NEB) following manufacturer’s recommended protocol. .. Quantify the recovered DNA using a Qubit fluorometer (Thermo Fisher Scientific).

    Plasmid Preparation:

    Article Title: Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior
    Article Snippet: .. All PCR products and plasmids were purified using Monarch DNA Gel Extraction Kit (NEB, United States) and MiniBEST Plasmid Purification Kit (Takara, Japan), respectively. ..

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs monarch nucleic acid purification kit
    Monarch Nucleic Acid Purification Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch nucleic acid purification kit/product/New England Biolabs
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    monarch nucleic acid purification kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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