monarch gdna purification columns  (New England Biolabs)


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    Name:
    Monarch gDNA Purification Columns
    Description:
    The Monarch gDNA Purification Columns are a component of the Monarch Genomic DNA Purification Kit and can be used to purify up to 30 µg of DNA from a wide variety of biological samples The columns are designed without the use of a retaining ring ensuring no buffer retention and no carryover of contaminants DNA can be eluted in as little as 35 μl for and is ready for use in a variety of downstream applications including RT PCR RNA Library Prep for NGS and transfection Monarch columns use less plastic than conventional columns reducing their environmental footprint without compromising performance They are supplied in quantities of 100 along with 200 Monarch Collection Tubes II
    Catalog Number:
    T3017L
    Price:
    130
    Category:
    Genomic DNA Purification Kit Components
    Size:
    100 columns
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    New England Biolabs monarch gdna purification columns
    Monarch gDNA Purification Columns
    The Monarch gDNA Purification Columns are a component of the Monarch Genomic DNA Purification Kit and can be used to purify up to 30 µg of DNA from a wide variety of biological samples The columns are designed without the use of a retaining ring ensuring no buffer retention and no carryover of contaminants DNA can be eluted in as little as 35 μl for and is ready for use in a variety of downstream applications including RT PCR RNA Library Prep for NGS and transfection Monarch columns use less plastic than conventional columns reducing their environmental footprint without compromising performance They are supplied in quantities of 100 along with 200 Monarch Collection Tubes II
    https://www.bioz.com/result/monarch gdna purification columns/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna purification columns - by Bioz Stars, 2021-06
    95/100 stars

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    Binding Assay:

    Article Title: Sequencing B cell receptors from ferrets (Mustela putorius furo)
    Article Snippet: Antibodies were purified using Pierce Protein A agarose (Thermo Fisher). .. Briefly, supernatants were equilibrated with Protein A IgG binding buffer and loaded into purification columns packed with 0.2mL of equilibrated Protein A agarose. .. Bound antibody was washed using Protein A IgG binding buffer (Thermo Fisher), and eluted with 15mL IgG elution buffer (Thermo Fisher) and neutralised with 1.5mL 1M TE buffer pH8.0 (Merck).

    Purification:

    Article Title: Sequencing B cell receptors from ferrets (Mustela putorius furo)
    Article Snippet: Antibodies were purified using Pierce Protein A agarose (Thermo Fisher). .. Briefly, supernatants were equilibrated with Protein A IgG binding buffer and loaded into purification columns packed with 0.2mL of equilibrated Protein A agarose. .. Bound antibody was washed using Protein A IgG binding buffer (Thermo Fisher), and eluted with 15mL IgG elution buffer (Thermo Fisher) and neutralised with 1.5mL 1M TE buffer pH8.0 (Merck).

    Article Title: Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes
    Article Snippet: .. Cells were then treated with lysostaphin for 1 hr at 37°C. gDNA was then extracted using the Monarch gDNA Purification Kit from NEB following the manufacturer’s instructions. .. Template preparation for TXTL Each TXTL reaction contained three template DNA sources: a sfGFP constitutive reporter plasmid (pUC), a plasmid expressing a sgRNA targeting GFP (pUC) using the SauCas9 sgRNA sequence from Strutt, et al. , an either a plasmid expressing SauCas9 under an arabinose promoter (p15A) or a genomic amplicon containing the Cas9 from S. haemolyticus or S. schleiferi.

    Article Title: Enterococcus faecalis Manganese Exporter MntE Alleviates Manganese Toxicity and Is Required for Mouse Gastrointestinal Colonization
    Article Snippet: The nucleotide sequence of mntE was obtained from the E. faecalis OG1RF genome via BioCyc ( ). .. The Wizard genomic DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) was used for purification of plasmids for gene expression and construction of the complement mutant. .. The Monarch DNA gel extraction kit (New England BioLabs, Ipswich, MA) was used to isolate PCR products during PCR.

    Article Title: The Unfolded Protein Response Modulates a Phosphoinositide-Binding Protein through the IRE1-bZIP60 Pathway 1The Unfolded Protein Response Modulates a Phosphoinositide-Binding Protein through the IRE1-bZIP60 Pathway 1 [OPEN]
    Article Snippet: .. MBP-fused recombinant proteins were purified according to the manual of the pMAL Protein Fusion and Purification System ( ). .. Briefly, E . coli C41(DE3) cells harboring pCY53 (MBP-SVB) or the pMAL-c5X vector control (MBP) were grown in Terrific Broth medium containing ampicillin (100 μg mL−1 ) and chloramphenicol (25 μg mL−1 ) at 37°C.

    Article Title: Enhancer features that drive formation of transcriptional condensates
    Article Snippet: 5’-fluorescently labeled (Cy5) M13(−21) (/5Cy5/ TGTAAAACGACGGCCAGT) and M13 reverse (/5Cy5/ CAGGAAACAGCTATGAC) primers (IDT) were used to PCR amplify the synthetic DNA sequence, yielding a fluorescently labeled PCR product. .. Fluorescent PCR products were gel-purified (Qiagen) and eluted products were further purified using NEB Monarch PCR purification to remove any residual contaminants. .. The octamer motif sequence “ATTTGCAT” from the immunoglobulin kappa promoter was used as the TF binding site.

    Article Title: Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes
    Article Snippet: .. Cells were then treated with lysostaphin for 1 h at 37 °C. gDNA was then extracted using the Monarch gDNA Purification Kit from NEB following the manufacturer’s instructions. ..

    Article Title: NOD1 rs2075820 (p.E266K) polymorphism is associated with gastric cancer among individuals infected with cagPAI-positive H. pylori
    Article Snippet: .. Genotyping Genomic DNA was isolated from blood samples using the salting out and Proteinase K method, or according to the protocol described by Chomczynski and Sacchi [ ] was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs, USA). .. Single nucleotide polymorphisms (SNPs) were genotyped at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands, by Infinium Global Screening Array-24 BeadChip (Illumina, USA) according to the manufacturer’s protocol.

    DNA Purification:

    Article Title: Reaction Kinetic Analysis of the 3-Hydroxypropionate/4-Hydroxybutyrate CO2 Fixation Cycle in Extremely Thermoacidophilic Archaea
    Article Snippet: .. Growth conditions for M. sedula (DSM 5348) and genomic DNA purification were conducted, as reported previously ( ). .. Strains and vectors used for cloning included the pET-46b EK/LIC cloning Kit, pRSF-2 Ek/LIC Vector Kit, pCDF-2 Ek/LIC Vector Kit, Novablue GigaSinglesTM E. coli competent cells (Novagen, San Diego, CA), and RosettaTM (DE3) E. coli competent cells (Stratagene, La Jolla, CA).

    Article Title: Enterococcus faecalis Manganese Exporter MntE Alleviates Manganese Toxicity and Is Required for Mouse Gastrointestinal Colonization
    Article Snippet: The nucleotide sequence of mntE was obtained from the E. faecalis OG1RF genome via BioCyc ( ). .. The Wizard genomic DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) was used for purification of plasmids for gene expression and construction of the complement mutant. .. The Monarch DNA gel extraction kit (New England BioLabs, Ipswich, MA) was used to isolate PCR products during PCR.

    Isolation:

    Article Title: Enterococcus faecalis Manganese Exporter MntE Alleviates Manganese Toxicity and Is Required for Mouse Gastrointestinal Colonization
    Article Snippet: The nucleotide sequence of mntE was obtained from the E. faecalis OG1RF genome via BioCyc ( ). .. The Wizard genomic DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) was used for purification of plasmids for gene expression and construction of the complement mutant. .. The Monarch DNA gel extraction kit (New England BioLabs, Ipswich, MA) was used to isolate PCR products during PCR.

    Article Title: NOD1 rs2075820 (p.E266K) polymorphism is associated with gastric cancer among individuals infected with cagPAI-positive H. pylori
    Article Snippet: .. Genotyping Genomic DNA was isolated from blood samples using the salting out and Proteinase K method, or according to the protocol described by Chomczynski and Sacchi [ ] was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs, USA). .. Single nucleotide polymorphisms (SNPs) were genotyped at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands, by Infinium Global Screening Array-24 BeadChip (Illumina, USA) according to the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: Enterococcus faecalis Manganese Exporter MntE Alleviates Manganese Toxicity and Is Required for Mouse Gastrointestinal Colonization
    Article Snippet: The nucleotide sequence of mntE was obtained from the E. faecalis OG1RF genome via BioCyc ( ). .. The Wizard genomic DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) was used for purification of plasmids for gene expression and construction of the complement mutant. .. The Monarch DNA gel extraction kit (New England BioLabs, Ipswich, MA) was used to isolate PCR products during PCR.

    Expressing:

    Article Title: Enterococcus faecalis Manganese Exporter MntE Alleviates Manganese Toxicity and Is Required for Mouse Gastrointestinal Colonization
    Article Snippet: The nucleotide sequence of mntE was obtained from the E. faecalis OG1RF genome via BioCyc ( ). .. The Wizard genomic DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) was used for purification of plasmids for gene expression and construction of the complement mutant. .. The Monarch DNA gel extraction kit (New England BioLabs, Ipswich, MA) was used to isolate PCR products during PCR.

    Mutagenesis:

    Article Title: Enterococcus faecalis Manganese Exporter MntE Alleviates Manganese Toxicity and Is Required for Mouse Gastrointestinal Colonization
    Article Snippet: The nucleotide sequence of mntE was obtained from the E. faecalis OG1RF genome via BioCyc ( ). .. The Wizard genomic DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) was used for purification of plasmids for gene expression and construction of the complement mutant. .. The Monarch DNA gel extraction kit (New England BioLabs, Ipswich, MA) was used to isolate PCR products during PCR.

    Recombinant:

    Article Title: The Unfolded Protein Response Modulates a Phosphoinositide-Binding Protein through the IRE1-bZIP60 Pathway 1The Unfolded Protein Response Modulates a Phosphoinositide-Binding Protein through the IRE1-bZIP60 Pathway 1 [OPEN]
    Article Snippet: .. MBP-fused recombinant proteins were purified according to the manual of the pMAL Protein Fusion and Purification System ( ). .. Briefly, E . coli C41(DE3) cells harboring pCY53 (MBP-SVB) or the pMAL-c5X vector control (MBP) were grown in Terrific Broth medium containing ampicillin (100 μg mL−1 ) and chloramphenicol (25 μg mL−1 ) at 37°C.

    Polymerase Chain Reaction:

    Article Title: Enhancer features that drive formation of transcriptional condensates
    Article Snippet: 5’-fluorescently labeled (Cy5) M13(−21) (/5Cy5/ TGTAAAACGACGGCCAGT) and M13 reverse (/5Cy5/ CAGGAAACAGCTATGAC) primers (IDT) were used to PCR amplify the synthetic DNA sequence, yielding a fluorescently labeled PCR product. .. Fluorescent PCR products were gel-purified (Qiagen) and eluted products were further purified using NEB Monarch PCR purification to remove any residual contaminants. .. The octamer motif sequence “ATTTGCAT” from the immunoglobulin kappa promoter was used as the TF binding site.

    Article Title: NOD1 rs2075820 (p.E266K) polymorphism is associated with gastric cancer among individuals infected with cagPAI-positive H. pylori
    Article Snippet: .. Genotyping Genomic DNA was isolated from blood samples using the salting out and Proteinase K method, or according to the protocol described by Chomczynski and Sacchi [ ] was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs, USA). .. Single nucleotide polymorphisms (SNPs) were genotyped at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands, by Infinium Global Screening Array-24 BeadChip (Illumina, USA) according to the manufacturer’s protocol.

    Salting Out:

    Article Title: NOD1 rs2075820 (p.E266K) polymorphism is associated with gastric cancer among individuals infected with cagPAI-positive H. pylori
    Article Snippet: .. Genotyping Genomic DNA was isolated from blood samples using the salting out and Proteinase K method, or according to the protocol described by Chomczynski and Sacchi [ ] was further purified using Monarch PCR and DNA cleanup columns (New England Biolabs, USA). .. Single nucleotide polymorphisms (SNPs) were genotyped at the Human Genomics Facility (HuGe-F) in Erasmus MC, Netherlands, by Infinium Global Screening Array-24 BeadChip (Illumina, USA) according to the manufacturer’s protocol.

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  • News
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  • Team
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  • Contact
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  • 95
    New England Biolabs monarch gdna purification columns
    Monarch Gdna Purification Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch gdna purification columns/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch gdna purification columns - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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