molony murine leukemia virus rnase h reverse transcriptase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    RNase H 5 U µL
    Description:
    Thermo Scientific Ribonuclease H RNase H specifically degrades the RNA strand in RNA DNA hybrids It does not hydrolyze the phosphodiester bonds within single stranded and double stranded DNA and RNA Applications• Removal of mRNA prior to synthesis of second strand cDNA• RT PCR and qRT PCR removal of RNA after first strand cDNA synthesis• Removal of the poly A sequences of mRNA after hybridization with oligo dT • Site specific cleavage of RNA• Studies of in vitro polyadenylation reaction products
    Catalog Number:
    en0201
    Price:
    None
    Applications:
    One-Step qRT-PCR|PCR & Real-Time PCR|RT-PCR|Real Time PCR (qPCR)|Reverse Transcription|Two-Step RT-PCR
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher molony murine leukemia virus rnase h reverse transcriptase
    Thermo Scientific Ribonuclease H RNase H specifically degrades the RNA strand in RNA DNA hybrids It does not hydrolyze the phosphodiester bonds within single stranded and double stranded DNA and RNA Applications• Removal of mRNA prior to synthesis of second strand cDNA• RT PCR and qRT PCR removal of RNA after first strand cDNA synthesis• Removal of the poly A sequences of mRNA after hybridization with oligo dT • Site specific cleavage of RNA• Studies of in vitro polyadenylation reaction products
    https://www.bioz.com/result/molony murine leukemia virus rnase h reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus rnase h reverse transcriptase - by Bioz Stars, 2020-11
    99/100 stars

    Images

    Related Articles

    Modification:

    Article Title: Increasing gene discovery and coverage using RNA-seq of globin RNA reduced porcine blood samples
    Article Snippet: .. Third, we evaluated the RNase H mediated GR method using porcine specific oligonucleotides modified from the Affymetrix GeneChip GR Protocol developed for reduction of human globin transcripts [ ]. .. We examined the sequence similarities of HBA and HBB , especially the oligonucleotide sequences on 3′ UTR, between human and pig using Clustal Omega (Additional file : Figure S2) [ ].

    Polymerase Chain Reaction:

    Article Title: Tumor Vascular Morphology Undergoes Dramatic Changes during Outgrowth of B16 Melanoma While Proangiogenic Gene Expression Remains Unchanged
    Article Snippet: .. Total RNA was subsequently reverse transcribed as described previously [ ], using Superscript III Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) in a 20 μ L final volume containing 250 ng of random hexamers (Promega, Madison, WI, USA) and 40 units of RNase OUT inhibitor (Invitrogen). mRNA expression analysis was performed in a real-time PCR-based, custom-designed, Low-Density Array set-up (Applied Biosystems, Foster City, CA, USA). ..

    Incubation:

    Article Title: Unstructured 5′-tails act through ribosome standby to override inhibitory structure at ribosome binding sites
    Article Snippet: .. RNase H (5 U; Thermo Scientific) and RNase H buffer (200 mM Tris–HCl [pH 7.8], 400 mM KCl, 80 mM MgCl2 , 10 mM DTT) were added to a total volume of 20 μl, and incubated for 15 min at 37°C. ..

    Article Title: m5C modification of mRNA serves a DNA damage code to promote homologous recombination
    Article Snippet: .. For RNaseA treatment: after heat treatment, cells were incubated with 100 μg/mL RNaseA in 100 μL RNase digestion buffer (5 mM EDTA, 300 mM NaCl, 10 mM Tris-HCl, pH 7.5) at room temperature for 25 min. For RNaseH1 treatment, the cells were incubated with 15 U RNaseH1 (Cat#: EN0201, ThermoFisher Scientific) in 100 μL reaction buffer (200 mM Tris-HCl, pH 7.8, 400 mM KCl, 80 mM MgCl2 , 10 mM DTT) at room temperature for 25 min. For DNase I treatment, cells were incubated with 20 U (1 μL) DNase I in 100 μL buffer (10 mM Tris-HCl, 2.5 mM MgCl2 , 0.5 mM CaCl2 , pH 7.5) at 37 °C for 30 min followed by heat treatment. .. After the nuclease treatments, the cells were blocked with 5% BSA in 0.1% PBST buffer and stained with m5C antibody.

    Expressing:

    Article Title: Tumor Vascular Morphology Undergoes Dramatic Changes during Outgrowth of B16 Melanoma While Proangiogenic Gene Expression Remains Unchanged
    Article Snippet: .. Total RNA was subsequently reverse transcribed as described previously [ ], using Superscript III Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) in a 20 μ L final volume containing 250 ng of random hexamers (Promega, Madison, WI, USA) and 40 units of RNase OUT inhibitor (Invitrogen). mRNA expression analysis was performed in a real-time PCR-based, custom-designed, Low-Density Array set-up (Applied Biosystems, Foster City, CA, USA). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher 5x mmulv buffer
    5x Mmulv Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5x mmulv buffer/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    5x mmulv buffer - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

    Image Search Results