molony murine leukemia virus reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher molony murine leukemia virus reverse transcriptase
    Molony Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-05
    99/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Characterization of LINE-1 Ribonucleoprotein Particles
    Article Snippet: .. Quantitative real-time PCR Quantitative PCR was performed on LEAP cDNA samples or M-MLV RT-PCR products using the 7300 Real Time PCR system (Applied Biosystems). .. For analysis, 1 µL of LEAP or M-MLV RT products was added to 19 µL of master mix (1X SYBR Green PCR Master Mix (Applied Biosystems), 500 nM L1 3′ end primer, and 500 nM L1 Reverse primer), and amplified in a standard Q-PCR run of 45 cycles.

    Amplification:

    Article Title: Characterization of LINE-1 Ribonucleoprotein Particles
    Article Snippet: .. For analysis, 1 µL of LEAP or M-MLV RT products was added to 19 µL of master mix (1X SYBR Green PCR Master Mix (Applied Biosystems), 500 nM L1 3′ end primer, and 500 nM L1 Reverse primer), and amplified in a standard Q-PCR run of 45 cycles. ..

    Transgenic Assay:

    Article Title: The Use of a Dexamethasone-inducible System to Synchronize Xa21 Expression to Study Rice Immunity
    Article Snippet: .. Kitaake) Transgenic rice seeds containing pTA7002::Myc::Xa21 ( ) Transgenic rice seeds containing Ubi::Myc::Xa21 ( ) Xanthomonas oryzae pv. oryzae ( Xoo ; Philippines race 6, strain PXO99Az) Dexamethasone (Sigma-Aldrich, catalog number: D1756) Dimethyl sulfoxide (DMSO) (Thermo Fisher Scientific, catalog number: D128-1) Tween-20 (Bio-Rad Laboratories, catalog number: 170-6531) Sterile H2 O (Milli-Q) TRIzol (Life Technologies, Invitrogen™ , catalog number: 15596-026) M-MLV reverse transcriptase (Life Technologies, Invitrogen™ , catalog number: 28025-013) SsoFastEvaGreenSupermix (Bio-Rad Laboratories, catalog number: 172-5203) Peptone sucrose agar (PSA) solid media containing 20 μg/ml cephalexin (MP Biomedicals, catalog number: 02150585) (see Recipes) Dexamethasone (see Recipes) Greenhouse rice growing conditions (see Recipes) Walk-in growth chamber rice growing conditions (see Recipes) .. Spray bottle (550 ml) (any supplier) for dexamethasone foliar spray 1.5 ml Eppendorf tube (any supplier) Surgical scissors (sharp/sharp, straight, 5 ½ inch or similar) for Xoo clipping inoculation 5 ½ inch square disposable pots Supertub (24 inch x 36 inch x 8 inch) (Mac Court Products, model: ST3608 or similar) Scale suitable for measurements down to 0.0001 g (any manufacturer) Spectrophotometer suitable for taking optical density measurements at 600 nm (any manufacturer) Growth chamber (14 h light and 10 h dark photoperiod with 28 °C temperature) (any manufacturer) for rice seed germination Incubation chamber (28 °C) (any manufacturer) for Xoo preparation Greenhouse capable of temperature and humidity control for growing rice plants Walk-in growth chamber (conviron or equivalent) for Xoo inoculation and dexamethasone treatment qPCR machine (Bio-Rad Laboratories, model: CFX96 Real-Time PCR)

    SYBR Green Assay:

    Article Title: Characterization of LINE-1 Ribonucleoprotein Particles
    Article Snippet: .. For analysis, 1 µL of LEAP or M-MLV RT products was added to 19 µL of master mix (1X SYBR Green PCR Master Mix (Applied Biosystems), 500 nM L1 3′ end primer, and 500 nM L1 Reverse primer), and amplified in a standard Q-PCR run of 45 cycles. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Characterization of LINE-1 Ribonucleoprotein Particles
    Article Snippet: .. Quantitative real-time PCR Quantitative PCR was performed on LEAP cDNA samples or M-MLV RT-PCR products using the 7300 Real Time PCR system (Applied Biosystems). .. For analysis, 1 µL of LEAP or M-MLV RT products was added to 19 µL of master mix (1X SYBR Green PCR Master Mix (Applied Biosystems), 500 nM L1 3′ end primer, and 500 nM L1 Reverse primer), and amplified in a standard Q-PCR run of 45 cycles.

    Polymerase Chain Reaction:

    Article Title: Gene structure and expression profile of Manduca sexta prophenoloxidase-activating proteinase-3 (PAP-3), an immune protein containing two clip domains
    Article Snippet: .. First-strand cDNA synthesis was performed using 2–4 µg RNA, 10 pmol oligo(dT)17 , and 200 U MMLV reverse transcriptase (Invitrogen Life Technologies) at 37 °C for 1 h. M. sexta ribosomal protein S3 transcripts were used as an internal standard to control the template amount in a preliminary PCR experiment. .. Relative levels of PAP-3 cDNA in the normalized samples were determined by semiquantitative PCR using j699 (5′-CGT GTT GTT ATT AGC TTC GTA TTT CT-3′) and j700 (5′-CAT CCC CCC AGC CTC TAC-3′ for PAP-3′.

    Article Title: Characterization of LINE-1 Ribonucleoprotein Particles
    Article Snippet: .. For analysis, 1 µL of LEAP or M-MLV RT products was added to 19 µL of master mix (1X SYBR Green PCR Master Mix (Applied Biosystems), 500 nM L1 3′ end primer, and 500 nM L1 Reverse primer), and amplified in a standard Q-PCR run of 45 cycles. ..

    Recombinant:

    Article Title: Gadd45a Is an RNA Binding Protein and Is Localized in Nuclear Speckles
    Article Snippet: .. Recombinant proteins used were bovine serum albumin (Fraction V, Sigma), His-Gadd45a and M-MLV-reverse transcriptase (Invitrogen). .. Binding reactions were performed in RNA binding buffer (10 mM Tris-HCl (pH 7.4), 10 mM KCl, 1 mM MgCl2 ).

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  • 99
    Thermo Fisher molony murine leukemia virus reverse transcriptase
    Molony Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    85
    Thermo Fisher molony murine leukemia virus reverse transcriptase enzyme
    Molony Murine Leukemia Virus Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukemia virus reverse transcriptase enzyme/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus reverse transcriptase enzyme - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

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