molony murine leukemia virus reverse transcriptase  (Promega)

 
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    Name:
    M MLV Reverse Transcriptase
    Description:
    RNA dependent DNA polymerase for cDNA synthesis
    Catalog Number:
    m1701
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis PCR RT PCR
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    Structured Review

    Promega molony murine leukemia virus reverse transcriptase
    RNA dependent DNA polymerase for cDNA synthesis
    https://www.bioz.com/result/molony murine leukemia virus reverse transcriptase/product/Promega
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    RT Lamp Assay:

    Article Title: Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
    Article Snippet: .. For each primer set, AMV and M-MLV RT-LAMP was performed and similar results were obtained, therefore, M-MLV reverse transcriptase was chosen for the subsequent RT-LAMP assay because of its relatively cheap price (Figure ). ..

    Purification:

    Article Title: APOBEC3A deaminates transiently exposed single-strand DNA during LINE-1 retrotransposition
    Article Snippet: .. RT-PCR was carried out on 0.5 μl of purified RNA, using MMLV reverse transcriptase (Promega, Madison, WI) and 0.8 μM LEAP adapter (5np1) at 42°C for 30 min. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: OsTIR1 and OsAFB2 Downregulation via OsmiR393 Overexpression Leads to More Tillers, Early Flowering and Less Tolerance to Salt and Drought in Rice
    Article Snippet: .. The total RNA reverse transcription reaction was performed with a two-step RT-PCR kit (Promega, Cat.# M 1701) on 2 µg of total RNA according to the product manual. .. Expression detection by real-time quantitative PCR Gene expression was analyzed by quantitative real time RT-PCR and semi-quantitative RT-PCR using the primers listed in .

    Article Title: APOBEC3A deaminates transiently exposed single-strand DNA during LINE-1 retrotransposition
    Article Snippet: .. RT-PCR was carried out on 0.5 μl of purified RNA, using MMLV reverse transcriptase (Promega, Madison, WI) and 0.8 μM LEAP adapter (5np1) at 42°C for 30 min. ..

    Incubation:

    Article Title: A cypovirus VP5 displays the RNA chaperone-like activity that destabilizes RNA helices and accelerates strand annealing
    Article Snippet: .. After incubation, 4 μl of the RT buffer, 1 μl of 0.1 M DTT, 5 U RNasin and 0.5 μl M-MLV reverse transcriptase (Promega) were supplemented and reacted at 25°C for 30 min. ..

    other:

    Article Title: Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
    Article Snippet: Discussion WYMV could be detected by the RT-LAMP method using the designed four sets of primers (I-IV) and M-MLV reverse transcriptase instead of AMV reverse transcriptase under isothermal condition at 65°C for 80 min.

    Polymerase Chain Reaction:

    Article Title: Calcium-dependent regulation of the cell cycle via a novel MAPK-NF-?B pathway in Swiss 3T3 cells
    Article Snippet: .. 2 μg of RNA was used to perform reverse transcriptase PCR (using 200 U M-MLV reverse transcriptase; Promega). .. For PCR amplification, 5 μl of RT products were incubated with 20 pmol of specific primers and 1 U of Taq DNA polymerase (Promega).

    Clear Native PAGE:

    Article Title: Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis
    Article Snippet: .. Native PAGE assays Five picomoles of RNAP and 25 pmol of HelD, T4 DNA ligase (TaKaRa) or MLV reverse transcriptase (Promega), respectively, were used. .. Proteins tested for mutual interactions were incubated for 15 min at 30°C in 10 μl in the storage buffer.

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  • 99
    Promega molony murine leukemia virus reverse transcriptase
    Molony Murine Leukemia Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukemia virus reverse transcriptase/product/Promega
    Average 99 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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