molony murine leukemia virus m mlv reverse transcriptase  (TaKaRa)

 
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    Name:
    MMLV Reverse Transcriptase
    Description:
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    Catalog Number:
    639575
    Price:
    None
    Size:
    8 000 Units
    Category:
    MMLV RT GPR Reverse transcriptases cDNA synthesis
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    Structured Review

    TaKaRa molony murine leukemia virus m mlv reverse transcriptase
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    https://www.bioz.com/result/molony murine leukemia virus m mlv reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus m mlv reverse transcriptase - by Bioz Stars, 2020-11
    99/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: ZIP4, a Novel Determinant of Tumor Invasion in Hepatocellular Carcinoma, Contributes to Tumor Recurrence after Liver Transplantation
    Article Snippet: .. Real-time polymerase chain reaction RNA was extracted using the TRIzol reagent (Invitrogen) and used for cDNA synthesis with a M-MLV Reverse Transcriptase (TaKaRa, Dalian, China). .. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) using the ABI Prism 7500 Real-time PCR System (Applied Biosystems).

    Article Title: Persistent Interactions with Bacterial Symbionts Direct Mature-Host Cell Morphology and Gene Expression in the Squid-Vibrio Symbiosis
    Article Snippet: .. Because of the increased presence of bacterial RNA during the daily population regrowth and based on preliminary RNA quantifications, total RNA quantities (500 µg) were adjusted for cDNA synthesis by a factor of 1.1 for the WT condition at 11 hdp and by a factor of 1.2 at 23 hpd. qRT-PCR procedures conform to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines ( ) and follow already-published protocols , with the following specifications. cDNAs were synthetized by the SMART Moloney murine leukemia virus (MMLV) reverse transcriptase (Clontech) from oligo(dT)12–18 primers (Life Technologies) and diluted 1/8-fold in water. qPCR mixes, consisting of 0.5 μl of each primer (10 μM), 5 μl of SsoAdvanced SYBR Green supermix (Bio-Rad), and 4 μl of a 1/8 dilution of the cDNA reaction mixture, were run on a CFX Bio-Rad instrument (2 technical replicates/biological replicate) as follows: 3 min at 94°C and 40 cycles of 10 s at 94°C, 10 s at 60°C, and 15 s at 72°C, with a melting curve from 70°C to 95°C. .. Primer sets (see ) exhibit PCR efficiencies between 92.3 and 101.9 (mean ± standard error [SE] = 97.5 ± 0.33), which were used to calculate expression values based on Pfaffl’s method ( ).

    Sequencing:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

    Agarose Gel Electrophoresis:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    Synthesized:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    Isolation:

    Article Title: Role of Heat Shock Proteases in Quorum-Sensing-Mediated Regulation of Biofilm Formation by Vibrio Species
    Article Snippet: .. Reverse transcription reactions were performed with the total RNA isolated from V. vulnificus , which had been harvested at several incubation time points, using Moloney murine leukemia virus (M-MLV) reverse transcriptase and a random 6-mer primer (TaKaRa). .. The cDNA was analyzed by quantitative RT-PCR using a LightCycler 480 II system (Roche).

    Spectrophotometry:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    SYBR Green Assay:

    Article Title: Persistent Interactions with Bacterial Symbionts Direct Mature-Host Cell Morphology and Gene Expression in the Squid-Vibrio Symbiosis
    Article Snippet: .. Because of the increased presence of bacterial RNA during the daily population regrowth and based on preliminary RNA quantifications, total RNA quantities (500 µg) were adjusted for cDNA synthesis by a factor of 1.1 for the WT condition at 11 hdp and by a factor of 1.2 at 23 hpd. qRT-PCR procedures conform to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines ( ) and follow already-published protocols , with the following specifications. cDNAs were synthetized by the SMART Moloney murine leukemia virus (MMLV) reverse transcriptase (Clontech) from oligo(dT)12–18 primers (Life Technologies) and diluted 1/8-fold in water. qPCR mixes, consisting of 0.5 μl of each primer (10 μM), 5 μl of SsoAdvanced SYBR Green supermix (Bio-Rad), and 4 μl of a 1/8 dilution of the cDNA reaction mixture, were run on a CFX Bio-Rad instrument (2 technical replicates/biological replicate) as follows: 3 min at 94°C and 40 cycles of 10 s at 94°C, 10 s at 60°C, and 15 s at 72°C, with a melting curve from 70°C to 95°C. .. Primer sets (see ) exhibit PCR efficiencies between 92.3 and 101.9 (mean ± standard error [SE] = 97.5 ± 0.33), which were used to calculate expression values based on Pfaffl’s method ( ).

    Concentration Assay:

    Article Title: Cynoglossus semilaevis Rspo3 Regulates Embryo Development by Inhibiting the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Adult tissue cDNA synthesis was performed with Reverse Transcriptase M-MLV Kit (TaKaRa, Dalian, China), while embryo cDNA was synthesized by PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). cDNA purity and concentration were determined by 1.5% agarose gel electrophoresis and UV spectrophotometry using NanoPhotometer Pearl (Implen, Schatzbogen, Germany). .. The ORF sequence of C. semilaevis Rspo3 was amplified using the TaKaRa Ex- Taq PCR kit and ligated to vector of pMD18-T (Takara).

    Incubation:

    Article Title: Role of Heat Shock Proteases in Quorum-Sensing-Mediated Regulation of Biofilm Formation by Vibrio Species
    Article Snippet: .. Reverse transcription reactions were performed with the total RNA isolated from V. vulnificus , which had been harvested at several incubation time points, using Moloney murine leukemia virus (M-MLV) reverse transcriptase and a random 6-mer primer (TaKaRa). .. The cDNA was analyzed by quantitative RT-PCR using a LightCycler 480 II system (Roche).

    Quantitative RT-PCR:

    Article Title: Persistent Interactions with Bacterial Symbionts Direct Mature-Host Cell Morphology and Gene Expression in the Squid-Vibrio Symbiosis
    Article Snippet: .. Because of the increased presence of bacterial RNA during the daily population regrowth and based on preliminary RNA quantifications, total RNA quantities (500 µg) were adjusted for cDNA synthesis by a factor of 1.1 for the WT condition at 11 hdp and by a factor of 1.2 at 23 hpd. qRT-PCR procedures conform to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines ( ) and follow already-published protocols , with the following specifications. cDNAs were synthetized by the SMART Moloney murine leukemia virus (MMLV) reverse transcriptase (Clontech) from oligo(dT)12–18 primers (Life Technologies) and diluted 1/8-fold in water. qPCR mixes, consisting of 0.5 μl of each primer (10 μM), 5 μl of SsoAdvanced SYBR Green supermix (Bio-Rad), and 4 μl of a 1/8 dilution of the cDNA reaction mixture, were run on a CFX Bio-Rad instrument (2 technical replicates/biological replicate) as follows: 3 min at 94°C and 40 cycles of 10 s at 94°C, 10 s at 60°C, and 15 s at 72°C, with a melting curve from 70°C to 95°C. .. Primer sets (see ) exhibit PCR efficiencies between 92.3 and 101.9 (mean ± standard error [SE] = 97.5 ± 0.33), which were used to calculate expression values based on Pfaffl’s method ( ).

    cDNA Library Assay:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

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  • 99
    TaKaRa molony murine leukemia virus m mlv reverse transcriptase
    Molony Murine Leukemia Virus M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukemia virus m mlv reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus m mlv reverse transcriptase - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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