molony murine leukaemia virus  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    SuperScript Plus Direct cDNA Labeling Module no purification
    Description:
    The SuperScript Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript III Reverse Transcriptase RT with the simplicity of direct labeling In place of an enzyme spike in the streamlined protocol incorporates balanced sets of novel array optimized nucleotides conjugated to Alexa Fluor 555 and 647 dyes This results in more reproducible array data with higher correlation coefficients than using standalone reagents The SuperScript Plus Direct cDNA Labeling System provides • Optimized all inclusive direct labeling with balanced aha Alexa Fluor nucleotides enabling higher numbers of array positives• Improved correlation coefficients for more accurate data Figure 1 • Low elution purification columns included for ease of useIn the SuperScript Plus cDNA Labeling method anchored oligo dT anneals to the mRNA template Figure 2 SuperScript III RT extends from the priming site incorporating dUTP labeled with Alexa Fluor dye directly to produce labeled cDNA Template RNA is degraded by base hydrolysis Free nucleotides and other contaminants are removed using low elution volume purification columns before hybridization to the microarray
    Catalog Number:
    l101504
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Labeling|Nucleic Acid Labeling & Oligo Synthesis|Gene Expression Analysis & Genotyping
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher molony murine leukaemia virus
    The SuperScript Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript III Reverse Transcriptase RT with the simplicity of direct labeling In place of an enzyme spike in the streamlined protocol incorporates balanced sets of novel array optimized nucleotides conjugated to Alexa Fluor 555 and 647 dyes This results in more reproducible array data with higher correlation coefficients than using standalone reagents The SuperScript Plus Direct cDNA Labeling System provides • Optimized all inclusive direct labeling with balanced aha Alexa Fluor nucleotides enabling higher numbers of array positives• Improved correlation coefficients for more accurate data Figure 1 • Low elution purification columns included for ease of useIn the SuperScript Plus cDNA Labeling method anchored oligo dT anneals to the mRNA template Figure 2 SuperScript III RT extends from the priming site incorporating dUTP labeled with Alexa Fluor dye directly to produce labeled cDNA Template RNA is degraded by base hydrolysis Free nucleotides and other contaminants are removed using low elution volume purification columns before hybridization to the microarray
    https://www.bioz.com/result/molony murine leukaemia virus/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    molony murine leukaemia virus - by Bioz Stars, 2020-11
    99/100 stars

    Related Products / Commonly Used Together

    rna
    random hexamer-primed reverse transcription

    Images

    Related Articles

    Synthesized:

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Origin and Evolution of the Sponge Aggregation Factor Gene Family
    Article Snippet: .. After DNase I treatment (Invitrogen), cDNA was synthesized from RNA using SuperScript III reverse transcriptase (Invitrogen) and oligo(dT)15 and random pentadecamer primers , according to manufacturer’s guidelines. ..

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. RT-PCR Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Fusarium oxysporum Triggers Tissue-Specific Transcriptional Reprogramming in Arabidopsis thaliana
    Article Snippet: .. F . oxysporum RT-qPCR First-strand cDNA was synthesized from 1μg RNA using SuperScript RNA H- Reverse Transcriptase (Invitrogen) and oligo (dT) primer, according to the manufacturer's instructions. .. RT-qPCR products were amplified in 10μl reactions containing 2μl cDNA, 2x SYBR® Green PCR Master Mix (Applied Biosystems) and 0.3 pmol primers.

    Article Title: OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae
    Article Snippet: .. First-stand cDNA was synthesized from total RNA using the Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA). .. RT-qPCR was performed using SYBR Green detection reagents (Quanta Biosciences, Carlsbad, CA, USA) in Applied Biosystem’s 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) with the primers (catBqF/catBqR, oxyRqF/oxyRqR), and gyrB was used as a reference gene (Additional file : Table S2).

    Isolation:

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. RT-PCR Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

    Quantitative RT-PCR:

    Article Title: Fusarium oxysporum Triggers Tissue-Specific Transcriptional Reprogramming in Arabidopsis thaliana
    Article Snippet: .. F . oxysporum RT-qPCR First-strand cDNA was synthesized from 1μg RNA using SuperScript RNA H- Reverse Transcriptase (Invitrogen) and oligo (dT) primer, according to the manufacturer's instructions. .. RT-qPCR products were amplified in 10μl reactions containing 2μl cDNA, 2x SYBR® Green PCR Master Mix (Applied Biosystems) and 0.3 pmol primers.

    Real-time Polymerase Chain Reaction:

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

    Cell Culture:

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

    Labeling:

    Article Title: Differential expression of MUC genes in endometrial and cervical tissues and tumors
    Article Snippet: .. The cDNA probes (MUC1 , MUC2 , MUC5B , MUC5AC , MUC8 and β-actin ) were labeled by the random priming technique using DECAprime II kit (Ambion, Austin, TX). .. Hybridization of 32 P-labeled cDNA probes was carried out at 42°C for 14–16 h. After hybridization, membranes were washed twice in 2× SSC and 0.1% SDS for 10 min. at room temperature, followed by two additional washes for 20 min at 65°C in 1× SSC and 0.1% SDS solution.

    Article Title: In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons
    Article Snippet: .. Thereafter, RNA was converted to cDNA using the Superscript Double-stranded cDNA Synthesis (Invitrogen) Kit and labeled with NimbleGen One Color DNA Labeling (NimbleGen, Roche). .. The labeled cDNA were hybridized to NimbleGen Human Gene Expression Array 12x135K (NimbleGen, Roche), which covers 45.033 genes with 3 probes per gene, containing 12 arrays per slide.

    DNA Labeling:

    Article Title: In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons
    Article Snippet: .. Thereafter, RNA was converted to cDNA using the Superscript Double-stranded cDNA Synthesis (Invitrogen) Kit and labeled with NimbleGen One Color DNA Labeling (NimbleGen, Roche). .. The labeled cDNA were hybridized to NimbleGen Human Gene Expression Array 12x135K (NimbleGen, Roche), which covers 45.033 genes with 3 probes per gene, containing 12 arrays per slide.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Phosphorylation of IRE1 at S729 regulates RIDD in B cells and antibody production after immunization
    Article Snippet: .. RT-PCR Total RNA was isolated with TRIzol reagent (Invitrogen). cDNA was synthesized from RNA with Superscript II reverse transcription (Invitrogen). .. The following sets of primers were used together with Platinum Taq DNA polymerase (Invitrogen) in PCR to detect the expression of mouse μS, 5′-CACACTGTACAATGTCTCCCT-3′ and 5′-AAAATGCAACATCTCACTCTG-3′; mouse UBC, 5′-CAGCCGTATATCTTCCCAGACT-3′ and 5′-CTCAGAGGGATGCCAGTAATCTA-3′; mouse XBP1s, 5′-GTCCATGGGAAGATGTTCTGG-3′ and 5′-CTGAGTCCGAATCAGGTGCAG-3′; mouse Hspa5, 5′-TGTCTTCTCAGCATCAAGCAAGG-3′ and 5′-CCAACACTTTCTGGACAGGCTT-3′; mouse Pdia4, 5′-GGGCTCTTTCAGGGAGATGG-3′ and 5′-GGGAGACTTTCAGGAACTTGGC-3′; mouse Itgb2, 5′-CTTTCCGAGAGCAACATCCAGC-3′ and 5′-GTTGCTGGAGTCGTCAGACAGT-3′; mouse Ergic3, 5′-GTTCAAGAAACGACTAGACAAGGA-3′ and 5′-ACCTCGACTTTCCCAAGCTC-3′; mouse Bloc1S1, 5′-GAGGAGAGAAGCTATCGCTGCA-3′ and 5′-CTGGACCTGTAGAGTCTTCACC-3′; mouse Tapbp1, 5′-ACCATTCCCAGGAACTCAAA-3′ and 5′-GAGAAGAAGGCTGTTGTTCTGG-3′; mouse Hgsnat, 5′-TCTCCGCTTTCTCCATTTTG-3′ and 5′-CGCATACACGTGGAAAGTCA-3′; mouse Scara3, 5′-TGCATGGATACTGACCCTGA-3′ and 5′-GCCGTGTTACCAGCTTCTTC-3′; mouse Col6a1, 5′-TGCTCAACATGAAGCAGACC-3′ and 5′-TTGAGGGAGAAAGCTCTGGA-3′; and mouse Pdgfrb, 5′-AACCCCCTTACAGCTGTCCT-3′ and 5′-TAATCCCGTCAGCATCTTCC-3′.

    Article Title: Polymorphisms in dipeptidyl peptidase 4 reduce host cell entry of Middle East respiratory syndrome coronavirus
    Article Snippet: .. In brief, viral RNA was isolated from cell culture supernatant using the NucleoSpin RNA Virus kit (Macherey-Nagel), reverse-transcribed into cDNA using the Superscript III one step RT–PCR system (ThermoFisher Scientific) and analyzed on a LightCycler 480 qPCR cycler platform (Roche) with primers and conditions as specified for the upE assay [ ]. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher molony murine leukemia virus reverse transcriptase
    Molony Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher molony murine leukaemia virus reverse transcriptase
    Molony Murine Leukaemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukaemia virus reverse transcriptase/product/Thermo Fisher
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    molony murine leukaemia virus reverse transcriptase - by Bioz Stars, 2020-11
    88/100 stars
      Buy from Supplier

    99
    Thermo Fisher molony murine leukemia virus reverse transcriptase enzyme
    Molony Murine Leukemia Virus Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molony murine leukemia virus reverse transcriptase enzyme/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    molony murine leukemia virus reverse transcriptase enzyme - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    Image Search Results