moloney murine leukemia virus reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 682 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr V? Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
    Article Snippet: First-strand cDNA synthesis was performed on 2 μg mRNA using an oligo (dT) primer (Promega) and moloney murine leukemia virus reverse transcriptase (GIBCO BRL/Life Technologies). .. 4% of the reaction was used as a template for PCR amplification.

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The 810-bp fragment containing part of 5′ UTR and VP0 was amplified by PCR, using 5 µL of cDNA in a 50-µL reaction mixture containing 50 mmol/L KCl, 10 mmol/L Tris/HCl (pH 8.5), 2.5 mmol/L MgCl2 , 0.2 mmol/L of each deoxynucleoside triphosphates, 50 pmol of each primer, and 1 U of Taq polymerase (Biotech International).

    Article Title: A Minimally Invasive Rabbit Model of Progressive and Reproducible Disc Degeneration Confirmed by Radiology, Gene Expression, and Histology
    Article Snippet: RNA was extracted in accordance with the manufacturer's instructions. cDNA was generated with Moloney murine leukemia virus reverse transcriptase (RT)(Invitrogen). .. The RT reaction was amplified in triplicate with real-time PCR (ABI PRISM 7900) in a final volume of 10 µL using SYBR Green Master Mix reagent at a final concentration of 1× (Applied Biosystems, Foster City, CA, USA).

    Positive Control:

    Article Title: Epidermal growth factor receptor-regulated miR-125a-5p - a metastatic inhibitor of lung cancer
    Article Snippet: Real-time quantitative RT-PCR Reverse transcription reactions were carried out using dNTP, Moloney murine leukemia virus reverse transcriptase and RiboLock ribonuclease inhibitor (Applied Biosystems, Foster City, CA, USA). .. The relative expression levels of miRNAs in each sample were calculated and quantified by using the 2−ΔΔCt method after normalization for expression of positive control.

    Synthesized:

    Article Title: Premature Aging in Skeletal Muscle Lacking Serum Response Factor
    Article Snippet: .. Quantitative RT-PCR Analysis Total RNA was extracted from Gastrocnemius muscles using a commercial kit (RNeasy, Qiagen) following the manufacturer's protocol. cDNA was synthesized from 2 µg of RNA using Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen) and random hexamers (Promega). .. Quantitative PCR analysis was performed using a Light Cycler (Roche) according to the manufacturer's instructions using a SYBR Green I kit (Roche).

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: .. Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The 810-bp fragment containing part of 5′ UTR and VP0 was amplified by PCR, using 5 µL of cDNA in a 50-µL reaction mixture containing 50 mmol/L KCl, 10 mmol/L Tris/HCl (pH 8.5), 2.5 mmol/L MgCl2 , 0.2 mmol/L of each deoxynucleoside triphosphates, 50 pmol of each primer, and 1 U of Taq polymerase (Biotech International).

    Quantitative RT-PCR:

    Article Title: Premature Aging in Skeletal Muscle Lacking Serum Response Factor
    Article Snippet: .. Quantitative RT-PCR Analysis Total RNA was extracted from Gastrocnemius muscles using a commercial kit (RNeasy, Qiagen) following the manufacturer's protocol. cDNA was synthesized from 2 µg of RNA using Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen) and random hexamers (Promega). .. Quantitative PCR analysis was performed using a Light Cycler (Roche) according to the manufacturer's instructions using a SYBR Green I kit (Roche).

    Article Title: The Receptor for Advanced Glycation End Products Is a Central Mediator of Asthma Pathogenesis
    Article Snippet: Paragraph title: qRT-PCR ... Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (Applied Biosystems, Foster City, CA) in a Techne thermal cycler (Bibby Scientific US, Burlington, NJ).

    Article Title: Unaltered myocilin expression in the blood of primary open angle glaucoma patients
    Article Snippet: Paragraph title: Quantitative RT–PCR ... The mixture was then cooled rapidly to 20 °C and then 10 μl 5× RT Buffer, 10 mM dithiothreitol, and 200 U Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen Life Sciences, Grand Island, NY) were added for a total volume of 50 μl.

    Article Title: Epidermal growth factor receptor-regulated miR-125a-5p - a metastatic inhibitor of lung cancer
    Article Snippet: .. Real-time quantitative RT-PCR Reverse transcription reactions were carried out using dNTP, Moloney murine leukemia virus reverse transcriptase and RiboLock ribonuclease inhibitor (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR was performed on an ABI PRISM 7300 Sequence Detection System (Applied Biosystems), using an SYBR Green I Real-Time PCR kit (GenePharma, Shanghai, China) for miR-24, miR-25, miR-29b, and miR-125a-5p, and TaqMan Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) for let-7i; 5s and RUN6B (Applied Biosystems) were used as positive controls.

    Article Title: Fgf21 Impairs Adipocyte Insulin Sensitivity in Mice Fed a Low-Carbohydrate, High-Fat Ketogenic Diet
    Article Snippet: 2.3 Reverse Transcription-quantitative Polymerase Chain Reaction Analysis Total RNA was extracted from mouse tissues using an RNeasy mini kit (Qiagen). cDNA was synthesized from the RNA (1 µg) as a template in a reaction mixture containing moloney murine leukemia virus reverse transcriptase (Gibco BRL) and a random hexadeoxynucleotide primer (Takara, Japan). .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed on a Thermal Cycler Dice (Takara), using SYBR Premix Ex Taq 2 (Takara).

    Real-time Polymerase Chain Reaction:

    Article Title: Early Metabolic Defects in Dexamethasone-Exposed and Undernourished Intrauterine Growth Restricted Rats
    Article Snippet: One microgram of pancreatic islets total RNA and five micrograms of liver, eWAT and iBAT total RNA were reverse-transcribed using 800 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen, Basel, Switzerland), in the presence of 0.3 units/µl RNAsin (Promega Corp, Madison, WI), 7.5 µM of random primers (oligo(dN)6), 1.2 mM dNTP and 12 µM of DTT. .. The expression of the cDNAs of interest were determined by quantitative real-time PCR using an ABI 7000 Sequence Detection System (Applera Europe, Rotkreuz, Switzerland) and were normalized using the housekeeping genes Ppia (cyclophilin) and Rplp0 (36B4).

    Article Title: Premature Aging in Skeletal Muscle Lacking Serum Response Factor
    Article Snippet: Quantitative RT-PCR Analysis Total RNA was extracted from Gastrocnemius muscles using a commercial kit (RNeasy, Qiagen) following the manufacturer's protocol. cDNA was synthesized from 2 µg of RNA using Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen) and random hexamers (Promega). .. Quantitative PCR analysis was performed using a Light Cycler (Roche) according to the manufacturer's instructions using a SYBR Green I kit (Roche).

    Article Title: Epidermal growth factor receptor-regulated miR-125a-5p - a metastatic inhibitor of lung cancer
    Article Snippet: Real-time quantitative RT-PCR Reverse transcription reactions were carried out using dNTP, Moloney murine leukemia virus reverse transcriptase and RiboLock ribonuclease inhibitor (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR was performed on an ABI PRISM 7300 Sequence Detection System (Applied Biosystems), using an SYBR Green I Real-Time PCR kit (GenePharma, Shanghai, China) for miR-24, miR-25, miR-29b, and miR-125a-5p, and TaqMan Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) for let-7i; 5s and RUN6B (Applied Biosystems) were used as positive controls.

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction. .. Real-time PCR was performed by using SYBR green master mix and ROX reference dye according to the manufacturer's instruction (Applied Bioscience, Foster City, CA).

    Article Title: Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression
    Article Snippet: The reverse transcription (RT) reaction was performed on 4 μg of purified total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation, Carlsbad, CA, USA) with oligo(dT) or random hexamer primers (Applied Biosystems, Foster City, CA, USA) for mRNA and heterogeneous nuclear RNA (hnRNA) studies, respectively. .. Real-time polymerase chain reaction (PCR) was performed using the SYBR Green PCR Master Mix kit (Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
    Article Snippet: The RNA (500 ng) was then used for cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and an oligo-dT primer as per the manufacturer's instructions. .. Gene expression was determined by quantitative real-time polymerase chain reaction (rtPCR) as described previously ( ).

    Article Title: A Minimally Invasive Rabbit Model of Progressive and Reproducible Disc Degeneration Confirmed by Radiology, Gene Expression, and Histology
    Article Snippet: RNA was extracted in accordance with the manufacturer's instructions. cDNA was generated with Moloney murine leukemia virus reverse transcriptase (RT)(Invitrogen). .. The RT reaction was amplified in triplicate with real-time PCR (ABI PRISM 7900) in a final volume of 10 µL using SYBR Green Master Mix reagent at a final concentration of 1× (Applied Biosystems, Foster City, CA, USA).

    Random Hexamer Labeling:

    Article Title: Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression
    Article Snippet: .. The reverse transcription (RT) reaction was performed on 4 μg of purified total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation, Carlsbad, CA, USA) with oligo(dT) or random hexamer primers (Applied Biosystems, Foster City, CA, USA) for mRNA and heterogeneous nuclear RNA (hnRNA) studies, respectively. .. The RT reactions were performed in a PTC-100 thermal cycler (MJ Research, now part of Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Expressing:

    Article Title: Early Metabolic Defects in Dexamethasone-Exposed and Undernourished Intrauterine Growth Restricted Rats
    Article Snippet: Paragraph title: Gene expression ... One microgram of pancreatic islets total RNA and five micrograms of liver, eWAT and iBAT total RNA were reverse-transcribed using 800 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen, Basel, Switzerland), in the presence of 0.3 units/µl RNAsin (Promega Corp, Madison, WI), 7.5 µM of random primers (oligo(dN)6), 1.2 mM dNTP and 12 µM of DTT.

    Article Title: The Receptor for Advanced Glycation End Products Is a Central Mediator of Asthma Pathogenesis
    Article Snippet: Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (Applied Biosystems, Foster City, CA) in a Techne thermal cycler (Bibby Scientific US, Burlington, NJ). .. The fold change in RAGE, IL-5, or IL-13 mRNA expression, compared with GAPDH mRNA housekeeping control, was determined with the ΔΔCt method.

    Article Title: Unaltered myocilin expression in the blood of primary open angle glaucoma patients
    Article Snippet: Quantitative RT–PCR A two-step semi-quantitative reverse transcriptase polymerase chain reaction (RT–PCR) method was used to measure gene expression levels of MYOC and β-globulin (HBB ) in POAG patients and controls. .. The mixture was then cooled rapidly to 20 °C and then 10 μl 5× RT Buffer, 10 mM dithiothreitol, and 200 U Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen Life Sciences, Grand Island, NY) were added for a total volume of 50 μl.

    Article Title: TLR Stimulation of Bone Marrow Lymphoid Precursors from Childhood Acute Leukemia Modifies Their Differentiation Potentials
    Article Snippet: .. Reverse-Transcription Polymerase Chain Reaction (RT-PCR) Analysis of Gene Expression To determine the expression of TLR1–10 transcripts in BM CD34+ cells from leukemic patients, mRNAs were isolated with Trizol (Invitrogen) and cDNAs were prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. The PCR was performed using ampli-Taq DNA polymerase with a step program of denaturation (94°C) for 30 seconds, annealing (60°C) for 30 seconds and elongation (72°C) for 1 minute.

    Article Title: Epidermal growth factor receptor-regulated miR-125a-5p - a metastatic inhibitor of lung cancer
    Article Snippet: Real-time quantitative RT-PCR Reverse transcription reactions were carried out using dNTP, Moloney murine leukemia virus reverse transcriptase and RiboLock ribonuclease inhibitor (Applied Biosystems, Foster City, CA, USA). .. The relative expression levels of miRNAs in each sample were calculated and quantified by using the 2−ΔΔCt method after normalization for expression of positive control.

    Article Title: Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
    Article Snippet: Paragraph title: Analysis of gene expression ... The RNA (500 ng) was then used for cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and an oligo-dT primer as per the manufacturer's instructions.

    Countercurrent Chromatography:

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction. .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control in all cases, and its primer sequence was 5′-AAC GGA TTT GGC CGT ATT GG-3′ and 5′-CTT CCC GTT CAG CTC TGG G-3′ (657 bp).

    Sequencing:

    Article Title: Early Metabolic Defects in Dexamethasone-Exposed and Undernourished Intrauterine Growth Restricted Rats
    Article Snippet: One microgram of pancreatic islets total RNA and five micrograms of liver, eWAT and iBAT total RNA were reverse-transcribed using 800 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen, Basel, Switzerland), in the presence of 0.3 units/µl RNAsin (Promega Corp, Madison, WI), 7.5 µM of random primers (oligo(dN)6), 1.2 mM dNTP and 12 µM of DTT. .. The expression of the cDNAs of interest were determined by quantitative real-time PCR using an ABI 7000 Sequence Detection System (Applera Europe, Rotkreuz, Switzerland) and were normalized using the housekeeping genes Ppia (cyclophilin) and Rplp0 (36B4).

    Article Title: T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr V? Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
    Article Snippet: Paragraph title: TCR Vβ PCR and Sequence Analysis. ... First-strand cDNA synthesis was performed on 2 μg mRNA using an oligo (dT) primer (Promega) and moloney murine leukemia virus reverse transcriptase (GIBCO BRL/Life Technologies).

    Article Title: The Receptor for Advanced Glycation End Products Is a Central Mediator of Asthma Pathogenesis
    Article Snippet: Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (Applied Biosystems, Foster City, CA) in a Techne thermal cycler (Bibby Scientific US, Burlington, NJ). .. The following sequence was performed on an ABI Prism 7300 machine (Applied Biosystems): 50°C (2 minutes), 95°C (10 minutes), and then 40 cycles of 95°C (15 seconds) followed by 60°C (1 minute).

    Article Title: Epidermal growth factor receptor-regulated miR-125a-5p - a metastatic inhibitor of lung cancer
    Article Snippet: Real-time quantitative RT-PCR Reverse transcription reactions were carried out using dNTP, Moloney murine leukemia virus reverse transcriptase and RiboLock ribonuclease inhibitor (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR was performed on an ABI PRISM 7300 Sequence Detection System (Applied Biosystems), using an SYBR Green I Real-Time PCR kit (GenePharma, Shanghai, China) for miR-24, miR-25, miR-29b, and miR-125a-5p, and TaqMan Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) for let-7i; 5s and RUN6B (Applied Biosystems) were used as positive controls.

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction. .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control in all cases, and its primer sequence was 5′-AAC GGA TTT GGC CGT ATT GG-3′ and 5′-CTT CCC GTT CAG CTC TGG G-3′ (657 bp).

    Cell Culture:

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: .. Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The 810-bp fragment containing part of 5′ UTR and VP0 was amplified by PCR, using 5 µL of cDNA in a 50-µL reaction mixture containing 50 mmol/L KCl, 10 mmol/L Tris/HCl (pH 8.5), 2.5 mmol/L MgCl2 , 0.2 mmol/L of each deoxynucleoside triphosphates, 50 pmol of each primer, and 1 U of Taq polymerase (Biotech International).

    Generated:

    Article Title: A Minimally Invasive Rabbit Model of Progressive and Reproducible Disc Degeneration Confirmed by Radiology, Gene Expression, and Histology
    Article Snippet: .. RNA was extracted in accordance with the manufacturer's instructions. cDNA was generated with Moloney murine leukemia virus reverse transcriptase (RT)(Invitrogen). .. The RT reaction was amplified in triplicate with real-time PCR (ABI PRISM 7900) in a final volume of 10 µL using SYBR Green Master Mix reagent at a final concentration of 1× (Applied Biosystems, Foster City, CA, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Unaltered myocilin expression in the blood of primary open angle glaucoma patients
    Article Snippet: Quantitative RT–PCR A two-step semi-quantitative reverse transcriptase polymerase chain reaction (RT–PCR) method was used to measure gene expression levels of MYOC and β-globulin (HBB ) in POAG patients and controls. .. The mixture was then cooled rapidly to 20 °C and then 10 μl 5× RT Buffer, 10 mM dithiothreitol, and 200 U Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen Life Sciences, Grand Island, NY) were added for a total volume of 50 μl.

    Article Title: TLR Stimulation of Bone Marrow Lymphoid Precursors from Childhood Acute Leukemia Modifies Their Differentiation Potentials
    Article Snippet: .. Reverse-Transcription Polymerase Chain Reaction (RT-PCR) Analysis of Gene Expression To determine the expression of TLR1–10 transcripts in BM CD34+ cells from leukemic patients, mRNAs were isolated with Trizol (Invitrogen) and cDNAs were prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. The PCR was performed using ampli-Taq DNA polymerase with a step program of denaturation (94°C) for 30 seconds, annealing (60°C) for 30 seconds and elongation (72°C) for 1 minute.

    Article Title: Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression
    Article Snippet: Paragraph title: Quantitative real-time reverse transcription-polymerase chain reaction ... The reverse transcription (RT) reaction was performed on 4 μg of purified total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation, Carlsbad, CA, USA) with oligo(dT) or random hexamer primers (Applied Biosystems, Foster City, CA, USA) for mRNA and heterogeneous nuclear RNA (hnRNA) studies, respectively.

    Article Title: Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
    Article Snippet: The RNA (500 ng) was then used for cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (Invitrogen) and an oligo-dT primer as per the manufacturer's instructions. .. Gene expression was determined by quantitative real-time polymerase chain reaction (rtPCR) as described previously ( ).

    Article Title: WNK4 is an essential effector of anterior formation in FGF signaling
    Article Snippet: .. RT-PCR analysis Total RNA was prepared using TRIzol (Invitrogen). cDNA synthesis was carried out using Moloney murine leukemia virus reverse transcriptase (Invitrogen). ..

    Injection:

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: .. Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The 810-bp fragment containing part of 5′ UTR and VP0 was amplified by PCR, using 5 µL of cDNA in a 50-µL reaction mixture containing 50 mmol/L KCl, 10 mmol/L Tris/HCl (pH 8.5), 2.5 mmol/L MgCl2 , 0.2 mmol/L of each deoxynucleoside triphosphates, 50 pmol of each primer, and 1 U of Taq polymerase (Biotech International).

    Recombinant:

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: .. Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The 810-bp fragment containing part of 5′ UTR and VP0 was amplified by PCR, using 5 µL of cDNA in a 50-µL reaction mixture containing 50 mmol/L KCl, 10 mmol/L Tris/HCl (pH 8.5), 2.5 mmol/L MgCl2 , 0.2 mmol/L of each deoxynucleoside triphosphates, 50 pmol of each primer, and 1 U of Taq polymerase (Biotech International).

    Isolation:

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: .. Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The 810-bp fragment containing part of 5′ UTR and VP0 was amplified by PCR, using 5 µL of cDNA in a 50-µL reaction mixture containing 50 mmol/L KCl, 10 mmol/L Tris/HCl (pH 8.5), 2.5 mmol/L MgCl2 , 0.2 mmol/L of each deoxynucleoside triphosphates, 50 pmol of each primer, and 1 U of Taq polymerase (Biotech International).

    Article Title: TLR Stimulation of Bone Marrow Lymphoid Precursors from Childhood Acute Leukemia Modifies Their Differentiation Potentials
    Article Snippet: .. Reverse-Transcription Polymerase Chain Reaction (RT-PCR) Analysis of Gene Expression To determine the expression of TLR1–10 transcripts in BM CD34+ cells from leukemic patients, mRNAs were isolated with Trizol (Invitrogen) and cDNAs were prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. The PCR was performed using ampli-Taq DNA polymerase with a step program of denaturation (94°C) for 30 seconds, annealing (60°C) for 30 seconds and elongation (72°C) for 1 minute.

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: .. Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction. .. Real-time PCR was performed by using SYBR green master mix and ROX reference dye according to the manufacturer's instruction (Applied Bioscience, Foster City, CA).

    Article Title: Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression
    Article Snippet: Total cellular RNA was isolated using the RNeasy Mini Kit (Qiagen Inc.) in accordance with the manufacturer's instructions, including DNA contamination removal by on-column treatment with the RNase-Free DNase Kit (Qiagen Inc.). .. The reverse transcription (RT) reaction was performed on 4 μg of purified total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation, Carlsbad, CA, USA) with oligo(dT) or random hexamer primers (Applied Biosystems, Foster City, CA, USA) for mRNA and heterogeneous nuclear RNA (hnRNA) studies, respectively.

    Purification:

    Article Title: T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr V? Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
    Article Snippet: T cells were Ficoll purified 3 d after restimulation with SWM 110–121, and PolyA+ –mRNA was prepared using the Microfast Track™ method (Invitrogen). .. First-strand cDNA synthesis was performed on 2 μg mRNA using an oligo (dT) primer (Promega) and moloney murine leukemia virus reverse transcriptase (GIBCO BRL/Life Technologies).

    Article Title: Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression
    Article Snippet: .. The reverse transcription (RT) reaction was performed on 4 μg of purified total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation, Carlsbad, CA, USA) with oligo(dT) or random hexamer primers (Applied Biosystems, Foster City, CA, USA) for mRNA and heterogeneous nuclear RNA (hnRNA) studies, respectively. .. The RT reactions were performed in a PTC-100 thermal cycler (MJ Research, now part of Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Early Metabolic Defects in Dexamethasone-Exposed and Undernourished Intrauterine Growth Restricted Rats
    Article Snippet: One microgram of pancreatic islets total RNA and five micrograms of liver, eWAT and iBAT total RNA were reverse-transcribed using 800 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen, Basel, Switzerland), in the presence of 0.3 units/µl RNAsin (Promega Corp, Madison, WI), 7.5 µM of random primers (oligo(dN)6), 1.2 mM dNTP and 12 µM of DTT. .. PCR products were quantified using the SYBR Green Core Reagent kit (Applera Europe, Rotkreuz, Switzerland) and results were expressed in arbitrary units (A.U) relative to the mean value of the control group.

    Article Title: T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr V? Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
    Article Snippet: Paragraph title: TCR Vβ PCR and Sequence Analysis. ... First-strand cDNA synthesis was performed on 2 μg mRNA using an oligo (dT) primer (Promega) and moloney murine leukemia virus reverse transcriptase (GIBCO BRL/Life Technologies).

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The 810-bp fragment containing part of 5′ UTR and VP0 was amplified by PCR, using 5 µL of cDNA in a 50-µL reaction mixture containing 50 mmol/L KCl, 10 mmol/L Tris/HCl (pH 8.5), 2.5 mmol/L MgCl2 , 0.2 mmol/L of each deoxynucleoside triphosphates, 50 pmol of each primer, and 1 U of Taq polymerase (Biotech International).

    Article Title: The Receptor for Advanced Glycation End Products Is a Central Mediator of Asthma Pathogenesis
    Article Snippet: Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (Applied Biosystems, Foster City, CA) in a Techne thermal cycler (Bibby Scientific US, Burlington, NJ). .. Quantitative RT-PCR (qRT-PCR) was performed with universal PCR buffer and TaqMan primer/probe assay reagent (Applied Biosystems) with primers for RAGE (Mm00545815 m1), IL-5 (Mm00439646 m1), IL-13 (Mm00505403 m1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control (Mm99999915 g1).

    Article Title: Unaltered myocilin expression in the blood of primary open angle glaucoma patients
    Article Snippet: Quantitative RT–PCR A two-step semi-quantitative reverse transcriptase polymerase chain reaction (RT–PCR) method was used to measure gene expression levels of MYOC and β-globulin (HBB ) in POAG patients and controls. .. The mixture was then cooled rapidly to 20 °C and then 10 μl 5× RT Buffer, 10 mM dithiothreitol, and 200 U Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen Life Sciences, Grand Island, NY) were added for a total volume of 50 μl.

    Article Title: TLR Stimulation of Bone Marrow Lymphoid Precursors from Childhood Acute Leukemia Modifies Their Differentiation Potentials
    Article Snippet: Reverse-Transcription Polymerase Chain Reaction (RT-PCR) Analysis of Gene Expression To determine the expression of TLR1–10 transcripts in BM CD34+ cells from leukemic patients, mRNAs were isolated with Trizol (Invitrogen) and cDNAs were prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. The PCR was performed using ampli-Taq DNA polymerase with a step program of denaturation (94°C) for 30 seconds, annealing (60°C) for 30 seconds and elongation (72°C) for 1 minute.

    Article Title: Epidermal growth factor receptor-regulated miR-125a-5p - a metastatic inhibitor of lung cancer
    Article Snippet: Real-time quantitative RT-PCR Reverse transcription reactions were carried out using dNTP, Moloney murine leukemia virus reverse transcriptase and RiboLock ribonuclease inhibitor (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR was performed on an ABI PRISM 7300 Sequence Detection System (Applied Biosystems), using an SYBR Green I Real-Time PCR kit (GenePharma, Shanghai, China) for miR-24, miR-25, miR-29b, and miR-125a-5p, and TaqMan Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) for let-7i; 5s and RUN6B (Applied Biosystems) were used as positive controls.

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: Paragraph title: 2.9. Polymerase Chain Reaction (PCR) Analysis ... Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction.

    Article Title: Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression
    Article Snippet: The reverse transcription (RT) reaction was performed on 4 μg of purified total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation, Carlsbad, CA, USA) with oligo(dT) or random hexamer primers (Applied Biosystems, Foster City, CA, USA) for mRNA and heterogeneous nuclear RNA (hnRNA) studies, respectively. .. Real-time polymerase chain reaction (PCR) was performed using the SYBR Green PCR Master Mix kit (Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: Fgf21 Impairs Adipocyte Insulin Sensitivity in Mice Fed a Low-Carbohydrate, High-Fat Ketogenic Diet
    Article Snippet: .. 2.3 Reverse Transcription-quantitative Polymerase Chain Reaction Analysis Total RNA was extracted from mouse tissues using an RNeasy mini kit (Qiagen). cDNA was synthesized from the RNA (1 µg) as a template in a reaction mixture containing moloney murine leukemia virus reverse transcriptase (Gibco BRL) and a random hexadeoxynucleotide primer (Takara, Japan). .. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed on a Thermal Cycler Dice (Takara), using SYBR Premix Ex Taq 2 (Takara).

    Article Title: Norjizak Injection: A Critical Risk for Transmitting Blood-Borne Infectious Diseases
    Article Snippet: Thereafter, C-DNA was produced by the use of Moloney Murine Leukemia Virus reverse transcriptase (Fermentas, Life Science). .. Using specific primers and a probe for the detection of HCV (Tagman probe), PCR was done (Corrbette Research 6000).

    Article Title: A Minimally Invasive Rabbit Model of Progressive and Reproducible Disc Degeneration Confirmed by Radiology, Gene Expression, and Histology
    Article Snippet: The tissues were immediately placed into liquid nitrogen and frozen at -80℃ in preparation for PCR analysis. .. RNA was extracted in accordance with the manufacturer's instructions. cDNA was generated with Moloney murine leukemia virus reverse transcriptase (RT)(Invitrogen).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Isolation and Characterization of Novel Human Parechovirus from Clinical Samples
    Article Snippet: Comparisons of VP0 Amino Acid Sequences of Clinical i solates To determine the VP0 amino acid sequences of viruses isolated from clinical samples with cultured cell lines, we extracted the viral RNA from culture supernatant of cells injected with the clinical samples by using the High Pure Viral RNA Kit (Roche, Mannheim, Germany). cDNA was synthesized from 8 µL of the viral RNA by using 1 U Moloney murine leukemia virus reverse transcriptase (Invitrogen) and 20 U of recombinant RNAs in ribonuclease inhibitor (Promega, Madison, WI, USA). .. The primer set used was E23P1 as a forward primer and HPV-N1 (5′-TAG GGG ATA CAT ARG TCR GCY T-3′ as a reverse primer ( ).

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction. .. The primers were as follows: 5′-GAC CTT GCT TTC CAT CAC CAC CGG-3′ and 5′-GTA GAG TGG TGA CTC CTC CCA GAC-3′ for NAD(P)H quinone oxidoreductase (NQO1; 241 bp); 5′-CCT GCT GTG TGA TGC CAC CAG ATT TT-3′ and 5′-TCT GCT TTT CAC GAT GAC CGA GTA CC-3′ for glutamate-cysteine ligase modulatory subunit (GCLM; 197 bp); 5′-CGT GGA CAC CCG ATG CAG TAT TCT G-3′ and 5′-GGG TCG CTT TTA CCT CCA CTG TAC T-3′ for glutamate-cysteine catalytic subunit (GCLC; 261 bp); 5′-CCT GGG CAT CTG AAA CCT TTT GAG AC-3′ and 5′-GCG AGC CAC ATA GGC AGA GAG C-3′ for glutathione S -transferase (GST; 180 bp).

    Software:

    Article Title: Early Metabolic Defects in Dexamethasone-Exposed and Undernourished Intrauterine Growth Restricted Rats
    Article Snippet: One microgram of pancreatic islets total RNA and five micrograms of liver, eWAT and iBAT total RNA were reverse-transcribed using 800 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen, Basel, Switzerland), in the presence of 0.3 units/µl RNAsin (Promega Corp, Madison, WI), 7.5 µM of random primers (oligo(dN)6), 1.2 mM dNTP and 12 µM of DTT. .. Primers sequences designed using the Primer Express software (Applera Europe, Rotkreuz, Switzerland) are listed in .

    SYBR Green Assay:

    Article Title: Early Metabolic Defects in Dexamethasone-Exposed and Undernourished Intrauterine Growth Restricted Rats
    Article Snippet: One microgram of pancreatic islets total RNA and five micrograms of liver, eWAT and iBAT total RNA were reverse-transcribed using 800 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen, Basel, Switzerland), in the presence of 0.3 units/µl RNAsin (Promega Corp, Madison, WI), 7.5 µM of random primers (oligo(dN)6), 1.2 mM dNTP and 12 µM of DTT. .. PCR products were quantified using the SYBR Green Core Reagent kit (Applera Europe, Rotkreuz, Switzerland) and results were expressed in arbitrary units (A.U) relative to the mean value of the control group.

    Article Title: Premature Aging in Skeletal Muscle Lacking Serum Response Factor
    Article Snippet: Quantitative RT-PCR Analysis Total RNA was extracted from Gastrocnemius muscles using a commercial kit (RNeasy, Qiagen) following the manufacturer's protocol. cDNA was synthesized from 2 µg of RNA using Moloney Murine Leukemia Virus reverse transcriptase (Invitrogen) and random hexamers (Promega). .. Quantitative PCR analysis was performed using a Light Cycler (Roche) according to the manufacturer's instructions using a SYBR Green I kit (Roche).

    Article Title: Epidermal growth factor receptor-regulated miR-125a-5p - a metastatic inhibitor of lung cancer
    Article Snippet: Real-time quantitative RT-PCR Reverse transcription reactions were carried out using dNTP, Moloney murine leukemia virus reverse transcriptase and RiboLock ribonuclease inhibitor (Applied Biosystems, Foster City, CA, USA). .. Real-time PCR was performed on an ABI PRISM 7300 Sequence Detection System (Applied Biosystems), using an SYBR Green I Real-Time PCR kit (GenePharma, Shanghai, China) for miR-24, miR-25, miR-29b, and miR-125a-5p, and TaqMan Universal PCR Master Mix, No AmpErase UNG (Applied Biosystems) for let-7i; 5s and RUN6B (Applied Biosystems) were used as positive controls.

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction. .. Real-time PCR was performed by using SYBR green master mix and ROX reference dye according to the manufacturer's instruction (Applied Bioscience, Foster City, CA).

    Article Title: Retinoid X receptor and peroxisome proliferator-activated receptor-gamma agonists cooperate to inhibit matrix metalloproteinase gene expression
    Article Snippet: The reverse transcription (RT) reaction was performed on 4 μg of purified total RNA using Moloney murine leukemia virus reverse transcriptase (Invitrogen Corporation, Carlsbad, CA, USA) with oligo(dT) or random hexamer primers (Applied Biosystems, Foster City, CA, USA) for mRNA and heterogeneous nuclear RNA (hnRNA) studies, respectively. .. Real-time polymerase chain reaction (PCR) was performed using the SYBR Green PCR Master Mix kit (Applied Biosystems) in accordance with the manufacturer's instructions.

    Article Title: A Minimally Invasive Rabbit Model of Progressive and Reproducible Disc Degeneration Confirmed by Radiology, Gene Expression, and Histology
    Article Snippet: RNA was extracted in accordance with the manufacturer's instructions. cDNA was generated with Moloney murine leukemia virus reverse transcriptase (RT)(Invitrogen). .. The RT reaction was amplified in triplicate with real-time PCR (ABI PRISM 7900) in a final volume of 10 µL using SYBR Green Master Mix reagent at a final concentration of 1× (Applied Biosystems, Foster City, CA, USA).

    RNA Extraction:

    Article Title: Norjizak Injection: A Critical Risk for Transmitting Blood-Borne Infectious Diseases
    Article Snippet: Two-hundred micro-liters of the solution in each vial were obtained and using a high pure viral nucleic acid kit, RNA extraction was done. .. Thereafter, C-DNA was produced by the use of Moloney Murine Leukemia Virus reverse transcriptase (Fermentas, Life Science).

    Agarose Gel Electrophoresis:

    Article Title: TLR Stimulation of Bone Marrow Lymphoid Precursors from Childhood Acute Leukemia Modifies Their Differentiation Potentials
    Article Snippet: Reverse-Transcription Polymerase Chain Reaction (RT-PCR) Analysis of Gene Expression To determine the expression of TLR1–10 transcripts in BM CD34+ cells from leukemic patients, mRNAs were isolated with Trizol (Invitrogen) and cDNAs were prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. PCR products were separated in a 1% (w/v) agarose gel, and visualized by staining with ethidium bromide.

    Produced:

    Article Title: Norjizak Injection: A Critical Risk for Transmitting Blood-Borne Infectious Diseases
    Article Snippet: .. Thereafter, C-DNA was produced by the use of Moloney Murine Leukemia Virus reverse transcriptase (Fermentas, Life Science). .. Using specific primers and a probe for the detection of HCV (Tagman probe), PCR was done (Corrbette Research 6000).

    Concentration Assay:

    Article Title: A Minimally Invasive Rabbit Model of Progressive and Reproducible Disc Degeneration Confirmed by Radiology, Gene Expression, and Histology
    Article Snippet: RNA was extracted in accordance with the manufacturer's instructions. cDNA was generated with Moloney murine leukemia virus reverse transcriptase (RT)(Invitrogen). .. The RT reaction was amplified in triplicate with real-time PCR (ABI PRISM 7900) in a final volume of 10 µL using SYBR Green Master Mix reagent at a final concentration of 1× (Applied Biosystems, Foster City, CA, USA).

    CTG Assay:

    Article Title: T Cell Receptor (Tcr)-Mediated Repertoire Selection and Loss of Tcr V? Diversity during the Initiation of a Cd4+ T Cell Response in Vivo
    Article Snippet: First-strand cDNA synthesis was performed on 2 μg mRNA using an oligo (dT) primer (Promega) and moloney murine leukemia virus reverse transcriptase (GIBCO BRL/Life Technologies). .. The 5′ TCR Vβ8 primer was 5′-TAA GCG GCC GCG AGG CTG CAG TCA-3′ and the 3′ Cβ primer was 5′-CAG CTC AGC TCC ACG TGG TC-3′.

    Article Title: Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
    Article Snippet: Total RNAs were isolated by TRIzol reagent (Invitrogen) and reverse-transcribed by Moloney Murine Leukemia Virus Reverse Transcriptase (Invitrogen) according to the manufacturer's instruction. .. The primers were as follows: 5′-GAC CTT GCT TTC CAT CAC CAC CGG-3′ and 5′-GTA GAG TGG TGA CTC CTC CCA GAC-3′ for NAD(P)H quinone oxidoreductase (NQO1; 241 bp); 5′-CCT GCT GTG TGA TGC CAC CAG ATT TT-3′ and 5′-TCT GCT TTT CAC GAT GAC CGA GTA CC-3′ for glutamate-cysteine ligase modulatory subunit (GCLM; 197 bp); 5′-CGT GGA CAC CCG ATG CAG TAT TCT G-3′ and 5′-GGG TCG CTT TTA CCT CCA CTG TAC T-3′ for glutamate-cysteine catalytic subunit (GCLC; 261 bp); 5′-CCT GGG CAT CTG AAA CCT TTT GAG AC-3′ and 5′-GCG AGC CAC ATA GGC AGA GAG C-3′ for glutathione S -transferase (GST; 180 bp).

    Staining:

    Article Title: TLR Stimulation of Bone Marrow Lymphoid Precursors from Childhood Acute Leukemia Modifies Their Differentiation Potentials
    Article Snippet: Reverse-Transcription Polymerase Chain Reaction (RT-PCR) Analysis of Gene Expression To determine the expression of TLR1–10 transcripts in BM CD34+ cells from leukemic patients, mRNAs were isolated with Trizol (Invitrogen) and cDNAs were prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen). .. PCR products were separated in a 1% (w/v) agarose gel, and visualized by staining with ethidium bromide.

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