moloney murine leukemia virus reverse transcriptase  (Stratagene)

 
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    Structured Review

    Stratagene moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Stratagene
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars

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    Amplification:

    Article Title: Endothelial ?1-adrenoceptors regulate neo-angiogenesis
    Article Snippet: .. RNA was then reverse transcribed into cDNA using Moloney murine leukemia virus Reverse Transcriptase (Stratagene) by standard methods ( ); cDNA samples were then used as templates for the PCR amplification using the pairs of specific primers reported in . .. Glyceraldehyde-3-phosphate dehydrogenase expression was used as loading and integrity control.

    Synthesized:

    Article Title: Polygalacturonase from Sitophilus oryzae: Possible horizontal transfer of a pectinase gene from fungi to weevils
    Article Snippet: .. The first strand of cDNA was synthesized using Moloney Murine Leukemia Virus reverse transcriptase according to the supplier's recommended conditions (Stratagene, www.stratagene.com ). .. This first strand cDNA was used as a template in PCR reactions using PG4 and a primer designated as PG3 [5′ GA(T/C)GA(T/C)GTIGCI(A/T)(C/G)NGC 3′ where I denotes deoxyinosine] based on the N-terminal amino acid sequence of the rice weevil polygalacturonase.

    Article Title: Impaired glucose homeostasis and neonatal mortality in hepatocyte nuclear factor 3?-deficient mice
    Article Snippet: .. Contaminating genomic DNA was removed by using DNase I treatment (20 units/10 μg of total RNA). cDNA was synthesized by using Moloney murine leukemia virus reverse transcriptase with dNTPs and random hexamer primers (Stratagene). ..

    Isolation:

    Article Title: CD28-B7-Mediated T Cell Costimulation in Chronic Cardiac Allograft Rejection
    Article Snippet: .. Total RNA was extracted from frozen tissue specimens using the Ultraspec RNA isolation system (Biotecx, Houston, TX) and was quantified by spectrophotometry. cDNA was prepared by reverse transcription of 5 μg of RNA using random hexamer primers (100 ng/μl) and Moloney murine leukemia virus reverse transcriptase (50 u/μl) (Stratagene, La Jolla, CA) in a 50-μl reaction. .. Ten microliters of cDNA were used for each PCR amplification reaction.

    Random Hexamer Labeling:

    Article Title: CD28-B7-Mediated T Cell Costimulation in Chronic Cardiac Allograft Rejection
    Article Snippet: .. Total RNA was extracted from frozen tissue specimens using the Ultraspec RNA isolation system (Biotecx, Houston, TX) and was quantified by spectrophotometry. cDNA was prepared by reverse transcription of 5 μg of RNA using random hexamer primers (100 ng/μl) and Moloney murine leukemia virus reverse transcriptase (50 u/μl) (Stratagene, La Jolla, CA) in a 50-μl reaction. .. Ten microliters of cDNA were used for each PCR amplification reaction.

    Article Title: Impaired glucose homeostasis and neonatal mortality in hepatocyte nuclear factor 3?-deficient mice
    Article Snippet: .. Contaminating genomic DNA was removed by using DNase I treatment (20 units/10 μg of total RNA). cDNA was synthesized by using Moloney murine leukemia virus reverse transcriptase with dNTPs and random hexamer primers (Stratagene). ..

    Spectrophotometry:

    Article Title: CD28-B7-Mediated T Cell Costimulation in Chronic Cardiac Allograft Rejection
    Article Snippet: .. Total RNA was extracted from frozen tissue specimens using the Ultraspec RNA isolation system (Biotecx, Houston, TX) and was quantified by spectrophotometry. cDNA was prepared by reverse transcription of 5 μg of RNA using random hexamer primers (100 ng/μl) and Moloney murine leukemia virus reverse transcriptase (50 u/μl) (Stratagene, La Jolla, CA) in a 50-μl reaction. .. Ten microliters of cDNA were used for each PCR amplification reaction.

    Incubation:

    Article Title: Gene Expression Analysis of Tumor Spheroids Reveals a Role for Suppressed DNA Mismatch Repair in Multicellular Resistance to Alkylating Agents
    Article Snippet: .. Two microliters of Moloney murine leukemia virus reverse transcriptase (50 U/μl; Stratagene) was added, and the reaction mixture was incubated at 37°C for 1 h. Reverse transcriptase was inactivated by incubation at 75°C for 5 min. Five microliters of each reaction mixture was made up to 50 μl of PCR volume containing 1× PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl, 1.5 mM MgCl2 ), 0.1 mM deoxynucleoside triphosphates, 2 μM concentrations of forward and reverse primers, and 1 U of Taq polymerase (Amersham). ..

    Polymerase Chain Reaction:

    Article Title: Endothelial ?1-adrenoceptors regulate neo-angiogenesis
    Article Snippet: .. RNA was then reverse transcribed into cDNA using Moloney murine leukemia virus Reverse Transcriptase (Stratagene) by standard methods ( ); cDNA samples were then used as templates for the PCR amplification using the pairs of specific primers reported in . .. Glyceraldehyde-3-phosphate dehydrogenase expression was used as loading and integrity control.

    Article Title: Gene Expression Analysis of Tumor Spheroids Reveals a Role for Suppressed DNA Mismatch Repair in Multicellular Resistance to Alkylating Agents
    Article Snippet: .. Two microliters of Moloney murine leukemia virus reverse transcriptase (50 U/μl; Stratagene) was added, and the reaction mixture was incubated at 37°C for 1 h. Reverse transcriptase was inactivated by incubation at 75°C for 5 min. Five microliters of each reaction mixture was made up to 50 μl of PCR volume containing 1× PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl, 1.5 mM MgCl2 ), 0.1 mM deoxynucleoside triphosphates, 2 μM concentrations of forward and reverse primers, and 1 U of Taq polymerase (Amersham). ..

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  • 92
    Stratagene moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/Stratagene
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Stratagene moloney murine leukemia virus
    Moloney Murine Leukemia Virus, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus/product/Stratagene
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    85
    Stratagene moloney murin leukaemia virus reverse transcriptase
    Moloney Murin Leukaemia Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murin leukaemia virus reverse transcriptase/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murin leukaemia virus reverse transcriptase - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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