moloney murine leukemia virus reverse transcriptase  (PerkinElmer)

 
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    Structured Review

    PerkinElmer moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/PerkinElmer
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Mycobacterial Antigens Exacerbate Disease Manifestations in Mycobacterium tuberculosis-Infected Mice
    Article Snippet: .. Briefly, 1 μg of RNA was reverse transcribed using a Moloney murine leukemia virus reverse transcriptase and was amplified with Taq polymerase according to procedures given in the GeneAmp RNA PCR kit (Perkin-Elmer, Branchburg, N.J.). ..

    Article Title: Expression of ?1D subunit mRNA is correlated with L-type Ca2+ channel activity in single neurons of hippocampal "zipper" slices
    Article Snippet: .. The collected cell was immediately transferred to a prechilled microcentrifuge tube containing RT solution: 1× PCR buffer (Perkin–Elmer), 3.75 mM MgCl2 , 0.5 mM each of dATP, dGTP, dCTP, and dTTP; 0.25 μM of random hexamers, 1 unit/ml RNase inhibitor (Roche Molecular Biochemicals), 0.5 μg/μl gene 32 protein, and 2.5 units Moloney murine leukemia virus reverse transcriptase (Perkin–Elmer). .. For cDNA synthesis, each cell was incubated at 42°C for 1 h in a total volume of 20 μl RT solution, followed by 10 min at 95°C.

    Article Title: Cadherin-6 Mediates the Heterotypic Interactions between the Hemopoietic Osteoclast Cell Lineage and Stromal Cells in a Murine Model of Osteoclast Differentiation
    Article Snippet: .. PCR detection of the alternatively spliced isoforms of cadherin-6 was performed either on human osteoclast-like cell cDNA library as described above or on reverse-transcribed single-strand cDNA from total RNA preparations (1 μg) using Moloney murine leukemia virus reverse transcriptase and random hexamers primers ( Perkin Elmer Cetus, Norwalk, CT). ..

    Article Title: Hypervariable Region 1 Sequence Stability during Hepatitis C Virus Replication in Chimpanzees
    Article Snippet: .. One-fifth of the extract was used to generate cDNA in a 20-μl reaction at 37°C for 1 h with 20 U of Moloney murine leukemia virus reverse transcriptase (Perkin-Elmer, Foster City, Calif.) and first-round PCR reverse primer. .. The entire 20-μl cDNA synthesis reaction was used for first-round PCR in a 25-μl reaction containing 0.625 U Expand HF polymerase mixture (Boehringer Mannheim, Indianapolis, Ind.), 1.5 mM MgCl2 , 0.2 mM deoxynucleoside triphosphates and 0.4 μM primers.

    Article Title: Immunopathologic Effects of Tumor Necrosis Factor Alpha in Murine Mycobacterial Infection Are Dose Dependent
    Article Snippet: .. Briefly, 1 μg of RNA was reverse transcribed with a Moloney murine leukemia virus reverse transcriptase and amplified with Taq polymerase according to procedures given in the GeneAmp RNA PCR kit (Perkin-Elmer, Branchburg, N.J.). ..

    cDNA Library Assay:

    Article Title: Cadherin-6 Mediates the Heterotypic Interactions between the Hemopoietic Osteoclast Cell Lineage and Stromal Cells in a Murine Model of Osteoclast Differentiation
    Article Snippet: .. PCR detection of the alternatively spliced isoforms of cadherin-6 was performed either on human osteoclast-like cell cDNA library as described above or on reverse-transcribed single-strand cDNA from total RNA preparations (1 μg) using Moloney murine leukemia virus reverse transcriptase and random hexamers primers ( Perkin Elmer Cetus, Norwalk, CT). ..

    Random Hexamer Labeling:

    Article Title: Reactive Oxygen Species Induce Antiviral Innate Immune Response through IFN-λ Regulation in Human Nasal Epithelial Cells
    Article Snippet: .. The cDNA was synthesized from 3 μg of RNA with random hexamer primers, using Moloney murine leukemia virus reverse transcriptase (PerkinElmer Life Sciences, Waltham, MA, and Roche Applied Science, Indianapolis, IN). .. Commercial reagents (TaqMan Universal PCR Master Mix; PE Biosystems, Foster City, CA) were selected and conditions were set according to the manufacturer’s protocol.

    Amplification:

    Article Title: Mycobacterial Antigens Exacerbate Disease Manifestations in Mycobacterium tuberculosis-Infected Mice
    Article Snippet: .. Briefly, 1 μg of RNA was reverse transcribed using a Moloney murine leukemia virus reverse transcriptase and was amplified with Taq polymerase according to procedures given in the GeneAmp RNA PCR kit (Perkin-Elmer, Branchburg, N.J.). ..

    Article Title: Immunopathologic Effects of Tumor Necrosis Factor Alpha in Murine Mycobacterial Infection Are Dose Dependent
    Article Snippet: .. Briefly, 1 μg of RNA was reverse transcribed with a Moloney murine leukemia virus reverse transcriptase and amplified with Taq polymerase according to procedures given in the GeneAmp RNA PCR kit (Perkin-Elmer, Branchburg, N.J.). ..

    Synthesized:

    Article Title: Reactive Oxygen Species Induce Antiviral Innate Immune Response through IFN-λ Regulation in Human Nasal Epithelial Cells
    Article Snippet: .. The cDNA was synthesized from 3 μg of RNA with random hexamer primers, using Moloney murine leukemia virus reverse transcriptase (PerkinElmer Life Sciences, Waltham, MA, and Roche Applied Science, Indianapolis, IN). .. Commercial reagents (TaqMan Universal PCR Master Mix; PE Biosystems, Foster City, CA) were selected and conditions were set according to the manufacturer’s protocol.

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    PerkinElmer mulv
    Mulv, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer moloney murine leukaemia virus reverse transcriptase
    Moloney Murine Leukaemia Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase/product/PerkinElmer
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    PerkinElmer moloney murine leukemia virus reverse transcriptase
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase/product/PerkinElmer
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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