moloney murine leukemia virus reverse transcriptase reagent kit  (Thermo Fisher)


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    Name:
    TaqMan Reverse Transcription Reagents
    Description:
    TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase PCR RT PCR Random hexamers oligo dT 16 and reverse primers are included for cDNA synthesis flexibility Advantages of TaqMan Reverse Transcription Reagents • Contain MultiScribe Reverse Transcriptase a recombinant moloney murine leukemia virus reverse transcriptase MMLV RT • Random hexamers and oligo dT 16 included for flexibility• Individual components provide versatility in assay set up• Compatible with two step RT PCR reactionsUsing TaqMan Reverse Transcription ReagentsTaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA For two step RT PCR random hexamers oligo dT 16 and sequence specific reverse primers can all be used The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems
    Catalog Number:
    4304134
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher moloney murine leukemia virus reverse transcriptase reagent kit
    TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase PCR RT PCR Random hexamers oligo dT 16 and reverse primers are included for cDNA synthesis flexibility Advantages of TaqMan Reverse Transcription Reagents • Contain MultiScribe Reverse Transcriptase a recombinant moloney murine leukemia virus reverse transcriptase MMLV RT • Random hexamers and oligo dT 16 included for flexibility• Individual components provide versatility in assay set up• Compatible with two step RT PCR reactionsUsing TaqMan Reverse Transcription ReagentsTaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA For two step RT PCR random hexamers oligo dT 16 and sequence specific reverse primers can all be used The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase reagent kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase reagent kit - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title:
    Article Snippet: .. TaqMan real-time reverse transcription–polymerase chain reaction (RT-PCR) was conducted using an ABI Prism 7700 system (Applied Biosystems) to determine OATP1B3 mRNA levels as described previously ( ). .. OATP1B3 expression relative to that of control was analyzed with the 2−ΔΔCT method ( ) using GAPDH as an internal control.

    Quantitative RT-PCR:

    Article Title: MiR-519d represses ovarian cancer cell proliferation and enhances cisplatin-mediated cytotoxicity in vitro by targeting XIAP
    Article Snippet: .. The expression levels of miR-519d in ovarian cancer cells and tissues were detected by TaqMan quantitative reverse transcriptase-polymerase chain reaction (TaqMan qRT-PCR; Life Technologies, Carlsbad, CA, USA). .. The effects of miR-519d on ovarian cancer cell proliferation and cisplatin chemosensitivity were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, flow cytometry, and Western blotting assay.

    Article Title: Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
    Article Snippet: .. Strand-specific RT-qPCR and Taqman small RNA assayFor strand-specific RT-qPCR, reverse transcription was performed using gene specific reverse transcription primers with a linker sequence at 5′ end, and then qPCR was performed using gene specific forward primer and the linker as reverse primer. .. Sequences of primers are listed in supplementary methods. qPCR analysis of human mascRNA was performed using Taqman small RNA assay (Life technologies, Assay ID: CSCSU4N), with U6 snRNA as internal loading control (Assay ID: 001973).

    Real-time Polymerase Chain Reaction:

    Article Title: Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
    Article Snippet: .. Strand-specific RT-qPCR and Taqman small RNA assayFor strand-specific RT-qPCR, reverse transcription was performed using gene specific reverse transcription primers with a linker sequence at 5′ end, and then qPCR was performed using gene specific forward primer and the linker as reverse primer. .. Sequences of primers are listed in supplementary methods. qPCR analysis of human mascRNA was performed using Taqman small RNA assay (Life technologies, Assay ID: CSCSU4N), with U6 snRNA as internal loading control (Assay ID: 001973).

    Article Title: Defining the Transcriptional and Cellular Landscape of Type 1 Diabetes in the NOD Mouse
    Article Snippet: .. Real Time PCR Complementary DNA was made from total RNA using TaqMan Reverse Transcription Reagents (Life Technologies) following the random hexamer protocol. .. Primers for quantitative RT-PCR for Cxcl9 , Stat1 , Gbp2 , Iigp1 , Rtp4 , Gpr18 , Oasl2 , and Tgtp1 were designed using Primer Bank ( http://pga.mgh . harvard.edu/primerbank/; .

    Article Title: A Vaccine Targeting Telomerase Enhances Survival of Dogs Affected by B-cell Lymphoma
    Article Snippet: .. One microgram of total RNA was used to perform reverse transcription of RNA and real-time PCR (TaqMan) assay using two primer/probe sets (Cf02671898_g1 and Cf02671897_m1) that recognized canine TERT (Applied Biosystems, Carlsbad, CA) according to manufacturer's instructions. .. The reactions were performed using a TaqMan Gold reverse transcription (RT)-PCR kit (PE Applied Biosystems) and included a 30-minute reverse transcription step at 50 °C followed by 10 minutes at 95 °C and 40 cycles of amplification using the universal TaqMan standardized conditions (15 seconds, 95 °C denaturation step followed by a 1-minute, 60 °C annealing/extension step).

    Sequencing:

    Article Title: Natural antisense RNA promotes 3′ end processing and maturation of MALAT1 lncRNA
    Article Snippet: .. Strand-specific RT-qPCR and Taqman small RNA assayFor strand-specific RT-qPCR, reverse transcription was performed using gene specific reverse transcription primers with a linker sequence at 5′ end, and then qPCR was performed using gene specific forward primer and the linker as reverse primer. .. Sequences of primers are listed in supplementary methods. qPCR analysis of human mascRNA was performed using Taqman small RNA assay (Life technologies, Assay ID: CSCSU4N), with U6 snRNA as internal loading control (Assay ID: 001973).

    Article Title: Expression and activity of eIF6 trigger Malignant Pleural Mesothelioma growth in vivo
    Article Snippet: .. Target mRNA quantification by quantitative reverse-transcriptase PCR using ΔΔCt-method using Taqman Universal PCR Master Mix (4304437; Life Technologies) was performed on an ABIPRISM 7900HT Sequence Detection System (Applied Biosystems). .. Cell proliferation, cell cycle and cell death analysis Proliferation rate of MPM cells was analysed by MTT test: briefly, cells were plated in 96 wells plates at different concentrations, and assayed after 24, 48 and 72 hours.

    Article Title: Respiratory Syncytial Virus Regulates Human MicroRNAs by Using Mechanisms Involving Beta Interferon and NF-?B
    Article Snippet: .. MicroRNAs were expanded by reverse transcription from 50 ng RNA with the TaqMan microRNA RT kit and sequence-specific primers (Applied Biosystems), as directed by the manufacturer. .. Copy DNA for the cellular control gene GAPDH was synthesized from 50 ng of RNA using a high-capacity cDNA reverse transcription kit (Applied Biosystems), as directed by the manufacturer.

    Random Hexamer Labeling:

    Article Title: Defining the Transcriptional and Cellular Landscape of Type 1 Diabetes in the NOD Mouse
    Article Snippet: .. Real Time PCR Complementary DNA was made from total RNA using TaqMan Reverse Transcription Reagents (Life Technologies) following the random hexamer protocol. .. Primers for quantitative RT-PCR for Cxcl9 , Stat1 , Gbp2 , Iigp1 , Rtp4 , Gpr18 , Oasl2 , and Tgtp1 were designed using Primer Bank ( http://pga.mgh . harvard.edu/primerbank/; .

    Expressing:

    Article Title: MiR-519d represses ovarian cancer cell proliferation and enhances cisplatin-mediated cytotoxicity in vitro by targeting XIAP
    Article Snippet: .. The expression levels of miR-519d in ovarian cancer cells and tissues were detected by TaqMan quantitative reverse transcriptase-polymerase chain reaction (TaqMan qRT-PCR; Life Technologies, Carlsbad, CA, USA). .. The effects of miR-519d on ovarian cancer cell proliferation and cisplatin chemosensitivity were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, flow cytometry, and Western blotting assay.

    Polymerase Chain Reaction:

    Article Title: Genome-wide promoter DNA Methylation Profiling of Hepatocellular Carcinomas Arising Either Spontaneously or due to Chronic Exposure to Ginkgo biloba Extract (GBE) in B6C3F1/N Mice
    Article Snippet: .. One microgram of total RNA was subjected to reverse-transcription PCR to generate complementary DNA (cDNA). .. Relative quantitative gene expression levels were detected using real-time PCR with the ABI PRISM 7900HT Sequence Detection System (Life Technologies, Grand Island, NY) using SYBR green detection methodology.

    Article Title: Expression and activity of eIF6 trigger Malignant Pleural Mesothelioma growth in vivo
    Article Snippet: .. Target mRNA quantification by quantitative reverse-transcriptase PCR using ΔΔCt-method using Taqman Universal PCR Master Mix (4304437; Life Technologies) was performed on an ABIPRISM 7900HT Sequence Detection System (Applied Biosystems). .. Cell proliferation, cell cycle and cell death analysis Proliferation rate of MPM cells was analysed by MTT test: briefly, cells were plated in 96 wells plates at different concentrations, and assayed after 24, 48 and 72 hours.

    TaqMan Assay:

    Article Title: A Vaccine Targeting Telomerase Enhances Survival of Dogs Affected by B-cell Lymphoma
    Article Snippet: .. One microgram of total RNA was used to perform reverse transcription of RNA and real-time PCR (TaqMan) assay using two primer/probe sets (Cf02671898_g1 and Cf02671897_m1) that recognized canine TERT (Applied Biosystems, Carlsbad, CA) according to manufacturer's instructions. .. The reactions were performed using a TaqMan Gold reverse transcription (RT)-PCR kit (PE Applied Biosystems) and included a 30-minute reverse transcription step at 50 °C followed by 10 minutes at 95 °C and 40 cycles of amplification using the universal TaqMan standardized conditions (15 seconds, 95 °C denaturation step followed by a 1-minute, 60 °C annealing/extension step).

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    Thermo Fisher moloney murine leukemia virus reverse transcriptase reagent kit
    Moloney Murine Leukemia Virus Reverse Transcriptase Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase reagent kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase reagent kit - by Bioz Stars, 2021-01
    99/100 stars
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