moloney murine leukemia virus reverse transcriptase master mix  (Thermo Fisher)


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    Structured Review

    Thermo Fisher moloney murine leukemia virus reverse transcriptase master mix
    Moloney Murine Leukemia Virus Reverse Transcriptase Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase master mix/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus reverse transcriptase master mix - by Bioz Stars, 2020-05
    93/100 stars

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    Polymerase Chain Reaction:

    Article Title: Artemisia princeps Inhibits Adipogenic Differentiation of 3T3-L1 Pre-Adipocytes via Downregulation of PPARγ and MAPK Pathways
    Article Snippet: .. Reverse transcription (RT)-polymerase chain reaction (PCR) assay Total RNA was isolated from mature 3T3-L1 adipocytes treated with APE fractions (day 0 to day 8) using Trizol reagent (Invitrogen, Carlsbad, CA, USA). cDNA synthesis was performed using 2 μg total RNA and a Moloney murine leukemia virus reverse transcriptase master mix (Invitrogen) containing 140 U reverse transcriptase, 1 mM deoxynucleoside triphosphates, and 500 ng oligo(dT), following manufacturer’s instructions. cDNA amplification was carried out using DNA polymerase containing ready to use master mix (Invitrogen) following the manufacturer’s recommended protocol. ..

    Isolation:

    Article Title: Artemisia princeps Inhibits Adipogenic Differentiation of 3T3-L1 Pre-Adipocytes via Downregulation of PPARγ and MAPK Pathways
    Article Snippet: .. Reverse transcription (RT)-polymerase chain reaction (PCR) assay Total RNA was isolated from mature 3T3-L1 adipocytes treated with APE fractions (day 0 to day 8) using Trizol reagent (Invitrogen, Carlsbad, CA, USA). cDNA synthesis was performed using 2 μg total RNA and a Moloney murine leukemia virus reverse transcriptase master mix (Invitrogen) containing 140 U reverse transcriptase, 1 mM deoxynucleoside triphosphates, and 500 ng oligo(dT), following manufacturer’s instructions. cDNA amplification was carried out using DNA polymerase containing ready to use master mix (Invitrogen) following the manufacturer’s recommended protocol. ..

    Amplification:

    Article Title: Artemisia princeps Inhibits Adipogenic Differentiation of 3T3-L1 Pre-Adipocytes via Downregulation of PPARγ and MAPK Pathways
    Article Snippet: .. Reverse transcription (RT)-polymerase chain reaction (PCR) assay Total RNA was isolated from mature 3T3-L1 adipocytes treated with APE fractions (day 0 to day 8) using Trizol reagent (Invitrogen, Carlsbad, CA, USA). cDNA synthesis was performed using 2 μg total RNA and a Moloney murine leukemia virus reverse transcriptase master mix (Invitrogen) containing 140 U reverse transcriptase, 1 mM deoxynucleoside triphosphates, and 500 ng oligo(dT), following manufacturer’s instructions. cDNA amplification was carried out using DNA polymerase containing ready to use master mix (Invitrogen) following the manufacturer’s recommended protocol. ..

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