moloney murine leukemia virus modified reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher moloney murine leukemia virus modified reverse transcriptase
    Moloney Murine Leukemia Virus Modified Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukemia virus modified reverse transcriptase/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus modified reverse transcriptase - by Bioz Stars, 2020-05
    85/100 stars

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    Modification:

    Article Title: vanE Gene Cluster of Vancomycin-Resistant Enterococcus faecalis BM4405
    Article Snippet: .. Labeled primer (1 pmol) was annealed to 50 μg of total RNA at 65°C for 3 min, and extension was performed in a 20-μl final volume with 40 U of Moloney murine leukemia virus modified reverse transcriptase (Superscript II; Gibco) for 45 min at 50°C. .. After addition of 5 μl of stop solution (Amersham Pharmacia Biotech) and heat denaturation, the sample was immediately loaded onto 6% polyacrylamide-urea sequencing gels for electrophoresis.

    Article Title: vanE Gene Cluster of Vancomycin-Resistant Enterococcus faecalis BM4405
    Article Snippet: .. Reverse transcription (RT) was carried out with 2 μg of purified RNA in a 20-μl final volume containing 1× enzyme buffer (Superscript II; Gibco), 50 mM magnesium chloride, 0.1 mg of bovine serum albumin (New England Biolabs Inc., Beverly, Mass.) per ml, 1 mM (each) of four deoxyribonucleoside triphosphates (Amersham Pharmacia Biotech), 50 pmol of the primer TE5 or PE3 (Fig. ; Table ), 20 U of RNase inhibitor (RNAguard; Amersham Pharmacia Biotech), and 200 U of Moloney murine leukemia virus modified reverse transcriptase (Superscript II; Gibco). .. The DNA products were amplified by PCR in an 80-μl reaction volume containing the previous 20-μl samples, 50 pmol each of the VDV and E15 primers or TE4 and PE3 (Fig. ; Table ), 1× enzyme buffer (Amersham Pharmacia Biotech), and 2 U of Taq DNA polymerase (Amersham Pharmacia Biotech).

    Article Title: Transcriptional Analysis of the vanC Cluster from Enterococcus gallinarum Strains with Constitutive and Inducible Vancomycin Resistance
    Article Snippet: .. Labeled PE1 was annealed to 50 μg of total RNA at 65°C for 3 min, and extension was performed with 40 U of Moloney murine leukemia virus modified reverse transcriptase (Superscript II; Gibco Life Technologies, Rockville, Md.) in a final volume of 20 μl for 45 min at 50°C. .. The reverse transcriptase products were analyzed by electrophoresis in 6% polyacrylamide-urea sequencing gels.

    Labeling:

    Article Title: vanE Gene Cluster of Vancomycin-Resistant Enterococcus faecalis BM4405
    Article Snippet: .. Labeled primer (1 pmol) was annealed to 50 μg of total RNA at 65°C for 3 min, and extension was performed in a 20-μl final volume with 40 U of Moloney murine leukemia virus modified reverse transcriptase (Superscript II; Gibco) for 45 min at 50°C. .. After addition of 5 μl of stop solution (Amersham Pharmacia Biotech) and heat denaturation, the sample was immediately loaded onto 6% polyacrylamide-urea sequencing gels for electrophoresis.

    Article Title: Transcriptional Analysis of the vanC Cluster from Enterococcus gallinarum Strains with Constitutive and Inducible Vancomycin Resistance
    Article Snippet: .. Labeled PE1 was annealed to 50 μg of total RNA at 65°C for 3 min, and extension was performed with 40 U of Moloney murine leukemia virus modified reverse transcriptase (Superscript II; Gibco Life Technologies, Rockville, Md.) in a final volume of 20 μl for 45 min at 50°C. .. The reverse transcriptase products were analyzed by electrophoresis in 6% polyacrylamide-urea sequencing gels.

    Purification:

    Article Title: vanE Gene Cluster of Vancomycin-Resistant Enterococcus faecalis BM4405
    Article Snippet: .. Reverse transcription (RT) was carried out with 2 μg of purified RNA in a 20-μl final volume containing 1× enzyme buffer (Superscript II; Gibco), 50 mM magnesium chloride, 0.1 mg of bovine serum albumin (New England Biolabs Inc., Beverly, Mass.) per ml, 1 mM (each) of four deoxyribonucleoside triphosphates (Amersham Pharmacia Biotech), 50 pmol of the primer TE5 or PE3 (Fig. ; Table ), 20 U of RNase inhibitor (RNAguard; Amersham Pharmacia Biotech), and 200 U of Moloney murine leukemia virus modified reverse transcriptase (Superscript II; Gibco). .. The DNA products were amplified by PCR in an 80-μl reaction volume containing the previous 20-μl samples, 50 pmol each of the VDV and E15 primers or TE4 and PE3 (Fig. ; Table ), 1× enzyme buffer (Amersham Pharmacia Biotech), and 2 U of Taq DNA polymerase (Amersham Pharmacia Biotech).

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  • 88
    Thermo Fisher recombinant moloney murine leukemia virus reverse transcriptase
    Recombinant Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant moloney murine leukemia virus reverse transcriptase/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant moloney murine leukemia virus reverse transcriptase - by Bioz Stars, 2020-05
    88/100 stars
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