moloney murine leukemia virus 152 reverse transcriptase  (Thermo Fisher)


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    Name:
    RevertAid Reverse Transcriptase 200 U µL
    Description:
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    Catalog Number:
    ep0441
    Price:
    None
    Applications:
    Cloning|PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher moloney murine leukemia virus 152 reverse transcriptase
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    https://www.bioz.com/result/moloney murine leukemia virus 152 reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukemia virus 152 reverse transcriptase - by Bioz Stars, 2020-12
    99/100 stars

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    Synthesized:

    Article Title: Moss Pathogenesis-Related-10 Protein Enhances Resistance to Pythium irregulare in Physcomitrella patens and Arabidopsis thaliana
    Article Snippet: .. For cDNA synthesis, 2 μg of total RNA was treated with RNasefree DNase I (Thermo Scientific) to remove genomic DNA contamination. cDNA was synthesized from total RNA using RevertAid Reverse transcriptase (Thermo Scientific) and oligo (dT) according to the manufacturer’s protocol. .. From the resulting 25 μL of cDNA, 2 μL were used as a template for PCR analysis using PpPR10 specific primers (PpPR-10f and PpPR-10r).

    Article Title: Inhibition of Autophagy Amplifies Baicalein-Induced Apoptosis in Human Colorectal Cancer
    Article Snippet: .. The cDNA was synthesized from RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific, USA). .. Thereafter, cDNA was amplified using the human apoptosis primer library (HPA-1; https://www.realtimeprimers.com/).

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Isolation:

    Article Title: Replacement of Murine Leukemia Virus Readthrough Mechanism by Human Immunodeficiency Virus Frameshift Allows Synthesis of Viral Proteins and Virus Replication
    Article Snippet: .. Briefly, the viral genomic RNA of chimeric or wild-type Mo-MuLV was isolated from resuspended viral particles and treated with fast-performance liquid chromatography pure DNase I (Amersham Pharmacia Biotech) before being used for the synthesis of a first DNA strand by using MuLV reverse transcriptase (Gibco-BRL) primed with an oligonucleotide located downstream of the Mfe I site (5′-gcc gta gga cag agg atg ag-3′). .. Amplification of double-stranded DNA was then achieved by using the same primer in combination with a second one located 5′ of the Xho I site (5′-gcc cca ttg gtc cca taa cc-3′).

    Quantitative RT-PCR:

    Article Title: Up-regulation of a cellular protein at the translational level by a retrovirus
    Article Snippet: .. RNA preparations were assessed for residual cellular DNA by standard PCRs using primers targeting the c-IAP1 gene. cDNA was prepared for qRT-PCR analysis by using the Mo-MLV reverse transcriptase enzyme (Invitrogen) and random hexamers as primers. .. SYBR-green-base qRT-PCR was used to assess relative c-IAP1 mRNA levels.

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    SYBR Green Assay:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Random Hexamer Labeling:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Polymerase Chain Reaction:

    Article Title: Long noncoding RNAs in vascular smooth muscle cells regulate vascular calcification
    Article Snippet: .. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) Complementary DNA (cDNA) was synthesized from total RNA using RevertAid Reverse Transcriptase (Thermo Fisher Scientific) and random hexamer primers (Thermo Fisher Scientific). qRT-PCR was conducted using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) in a Rotor-Gene Q (Qiagen). .. The primer sequences used for PCR are listed in Supplementary Table .

    Liquid Chromatography:

    Article Title: Replacement of Murine Leukemia Virus Readthrough Mechanism by Human Immunodeficiency Virus Frameshift Allows Synthesis of Viral Proteins and Virus Replication
    Article Snippet: .. Briefly, the viral genomic RNA of chimeric or wild-type Mo-MuLV was isolated from resuspended viral particles and treated with fast-performance liquid chromatography pure DNase I (Amersham Pharmacia Biotech) before being used for the synthesis of a first DNA strand by using MuLV reverse transcriptase (Gibco-BRL) primed with an oligonucleotide located downstream of the Mfe I site (5′-gcc gta gga cag agg atg ag-3′). .. Amplification of double-stranded DNA was then achieved by using the same primer in combination with a second one located 5′ of the Xho I site (5′-gcc cca ttg gtc cca taa cc-3′).

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  • 99
    Thermo Fisher m mulv reverse transcriptase
    M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 1038 article reviews
    Price from $9.99 to $1999.99
    m mulv reverse transcriptase - by Bioz Stars, 2020-12
    99/100 stars
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