moloney murine leukaemia virus reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher moloney murine leukaemia virus reverse transcriptase kit
    Moloney Murine Leukaemia Virus Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase kit/product/Thermo Fisher
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase kit - by Bioz Stars, 2020-05
    88/100 stars

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    Related Articles

    Synthesized:

    Article Title: Short-term dietary phosphate restriction up-regulates ileal fibroblast growth factor 15 gene expression in mice
    Article Snippet: .. Quantitative RT-PCR analysis First-strand cDNA was synthesized from DNase I-treated total RNA templates that were primed with oligo-dT using the Moloney-murine-leukaemia virus-reverse transcriptase kit from Invitrogen (San Diego, CA). .. Quantitative RT-PCR was performed using the Light Cycler system (Roche Diagnostics, Mannheim, Germany) or StepOnePlusTM Real-Time PCR System with FastStart SYBR green master mix (Applied Biosystems, Foster City, CA).

    Isolation:

    Article Title: Cathelicidin- derived PR39 protects enterohemorrhagic Escherichia coli O157:H7 challenged mice by improving epithelial function and balancing the microbiota in the intestine
    Article Snippet: .. Real-time PCR for gene expression analysis The qRT-PCR analysis procedure was carried out referring to our published work , to introduce briefly, RNA samples (n = 6 per group) were extracted from the middle jejunum tissues, total RNA isolation and cDNA synthesis by reverse transcription were conducted using Trizol reagent (Invitrogen Carlsbad CA USA) and M-MuLV reverse transcriptase kit (Fermentas EU Glen BURNIE USA) respectively. .. The mRNA levels of individual genes were measured by real-time PCR using the SYBR Premix Ex Taq Kit (Takara Biotechnology Shiga Japan) in the ABI Step One Plus Real-Time PCR system (Applied Biosystems CA USA), data was analyzed according to the comparative threshold cycle (Ct) method and normalized to an endogenous reference GAPDH.

    Quantitative RT-PCR:

    Article Title: Short-term dietary phosphate restriction up-regulates ileal fibroblast growth factor 15 gene expression in mice
    Article Snippet: .. Quantitative RT-PCR analysis First-strand cDNA was synthesized from DNase I-treated total RNA templates that were primed with oligo-dT using the Moloney-murine-leukaemia virus-reverse transcriptase kit from Invitrogen (San Diego, CA). .. Quantitative RT-PCR was performed using the Light Cycler system (Roche Diagnostics, Mannheim, Germany) or StepOnePlusTM Real-Time PCR System with FastStart SYBR green master mix (Applied Biosystems, Foster City, CA).

    Article Title: Cathelicidin- derived PR39 protects enterohemorrhagic Escherichia coli O157:H7 challenged mice by improving epithelial function and balancing the microbiota in the intestine
    Article Snippet: .. Real-time PCR for gene expression analysis The qRT-PCR analysis procedure was carried out referring to our published work , to introduce briefly, RNA samples (n = 6 per group) were extracted from the middle jejunum tissues, total RNA isolation and cDNA synthesis by reverse transcription were conducted using Trizol reagent (Invitrogen Carlsbad CA USA) and M-MuLV reverse transcriptase kit (Fermentas EU Glen BURNIE USA) respectively. .. The mRNA levels of individual genes were measured by real-time PCR using the SYBR Premix Ex Taq Kit (Takara Biotechnology Shiga Japan) in the ABI Step One Plus Real-Time PCR system (Applied Biosystems CA USA), data was analyzed according to the comparative threshold cycle (Ct) method and normalized to an endogenous reference GAPDH.

    Article Title: De novo transcriptome sequencing of two cultivated jute species under salinity stress
    Article Snippet: .. For qRT-PCR, total RNA was reverse transcribed into first-strand cDNA using the M-MuLV Reverse Transcriptase kit (Fermentas, Burlington, ON, Canada) according to the manufacturer’s instructions. .. The reaction was performed using the FastStart Universal SYBR Green Master kit (Roche Diagnostics, Basel, Switzerland) according to the manufacturer’s instructions in an optical 384-well plate on a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Cathelicidin- derived PR39 protects enterohemorrhagic Escherichia coli O157:H7 challenged mice by improving epithelial function and balancing the microbiota in the intestine
    Article Snippet: .. Real-time PCR for gene expression analysis The qRT-PCR analysis procedure was carried out referring to our published work , to introduce briefly, RNA samples (n = 6 per group) were extracted from the middle jejunum tissues, total RNA isolation and cDNA synthesis by reverse transcription were conducted using Trizol reagent (Invitrogen Carlsbad CA USA) and M-MuLV reverse transcriptase kit (Fermentas EU Glen BURNIE USA) respectively. .. The mRNA levels of individual genes were measured by real-time PCR using the SYBR Premix Ex Taq Kit (Takara Biotechnology Shiga Japan) in the ABI Step One Plus Real-Time PCR system (Applied Biosystems CA USA), data was analyzed according to the comparative threshold cycle (Ct) method and normalized to an endogenous reference GAPDH.

    Article Title: Involvement of SET in the Wnt signaling pathway and the development of human colorectal cancer
    Article Snippet: .. The total RNA was reverse-transcribed using the M-MuLV Reverse Transcriptase kit (Fermentas International Inc.) and qPCR was performed using SYBR Premix ExTaq (Takara). qPCR was performed on the the tumor and matched normal tissues of patients with colorectal cancer. ..

    Introduce:

    Article Title: Cathelicidin- derived PR39 protects enterohemorrhagic Escherichia coli O157:H7 challenged mice by improving epithelial function and balancing the microbiota in the intestine
    Article Snippet: .. Real-time PCR for gene expression analysis The qRT-PCR analysis procedure was carried out referring to our published work , to introduce briefly, RNA samples (n = 6 per group) were extracted from the middle jejunum tissues, total RNA isolation and cDNA synthesis by reverse transcription were conducted using Trizol reagent (Invitrogen Carlsbad CA USA) and M-MuLV reverse transcriptase kit (Fermentas EU Glen BURNIE USA) respectively. .. The mRNA levels of individual genes were measured by real-time PCR using the SYBR Premix Ex Taq Kit (Takara Biotechnology Shiga Japan) in the ABI Step One Plus Real-Time PCR system (Applied Biosystems CA USA), data was analyzed according to the comparative threshold cycle (Ct) method and normalized to an endogenous reference GAPDH.

    Expressing:

    Article Title: Cathelicidin- derived PR39 protects enterohemorrhagic Escherichia coli O157:H7 challenged mice by improving epithelial function and balancing the microbiota in the intestine
    Article Snippet: .. Real-time PCR for gene expression analysis The qRT-PCR analysis procedure was carried out referring to our published work , to introduce briefly, RNA samples (n = 6 per group) were extracted from the middle jejunum tissues, total RNA isolation and cDNA synthesis by reverse transcription were conducted using Trizol reagent (Invitrogen Carlsbad CA USA) and M-MuLV reverse transcriptase kit (Fermentas EU Glen BURNIE USA) respectively. .. The mRNA levels of individual genes were measured by real-time PCR using the SYBR Premix Ex Taq Kit (Takara Biotechnology Shiga Japan) in the ABI Step One Plus Real-Time PCR system (Applied Biosystems CA USA), data was analyzed according to the comparative threshold cycle (Ct) method and normalized to an endogenous reference GAPDH.

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    Thermo Fisher moloney murine leukemia virus m mulv reverse transcriptase kit
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    Thermo Fisher moloney murine leukemia virus reverse transcriptase kit
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    https://www.bioz.com/result/moloney murine leukemia virus reverse transcriptase kit/product/Thermo Fisher
    Average 93 stars, based on 20 article reviews
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    moloney murine leukemia virus reverse transcriptase kit - by Bioz Stars, 2020-05
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