moloney murine leukaemia virus reverse transcriptase enzyme  (Thermo Fisher)


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    Structured Review

    Thermo Fisher moloney murine leukaemia virus reverse transcriptase enzyme
    Moloney Murine Leukaemia Virus Reverse Transcriptase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/moloney murine leukaemia virus reverse transcriptase enzyme/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    moloney murine leukaemia virus reverse transcriptase enzyme - by Bioz Stars, 2020-05
    85/100 stars

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    DNA Synthesis:

    Article Title: Immunomodulatory effects of the tobacco-specific carcinogen, NNK, on alveolar macrophages
    Article Snippet: .. For complementary DNA synthesis, 1 µg of total RNA was reverse transcribed by Moloney murine leukaemia virus reverse transcriptase enzyme ( GIBCO RBL) using a Peltier Thermal Cycler 200 (MJ Research Inc., Watertown, MA) according to the manufacturer's protocol. .. PCR amplification was performed using the Qiagen Taq DNA polymerase protocol in a 20-µl final volume.

    Synthesized:

    Article Title: MicroRNA-148b and microRNA-152 reactivate tumor suppressor genes through suppression of DNA methyltransferase-1 gene in pancreatic cancer cell lines
    Article Snippet: .. RNA concentration was determined by spectrophotometer (IMPLEN,) and RNA quality was evaluated by determining the A260/A280 ratio. cDNA was synthesized by M-MuLV reverse transcriptase enzyme with 1 µg RNA using the cDNA synthesis kit (Fermentas, EP0351) according to the manufacturer’s recommendations. ..

    Isolation:

    Article Title: Systemic Monocytic-MDSCs Are Generated from Monocytes and Correlate with Disease Progression in Breast Cancer Patients
    Article Snippet: .. Quantitative RT-PCR Total RNA from monocytes was isolated using TRIZOL. cDNA synthesis was performed using random hexamers and the M-MuLV reverse transcriptase enzyme (Thermo Scientific). .. Quantitative RT-PCR was performed in triplicates according to the manufacturer’s instructions using Maxima SYBR Green/Rox (Thermo Scientific).

    Quantitative RT-PCR:

    Article Title: Systemic Monocytic-MDSCs Are Generated from Monocytes and Correlate with Disease Progression in Breast Cancer Patients
    Article Snippet: .. Quantitative RT-PCR Total RNA from monocytes was isolated using TRIZOL. cDNA synthesis was performed using random hexamers and the M-MuLV reverse transcriptase enzyme (Thermo Scientific). .. Quantitative RT-PCR was performed in triplicates according to the manufacturer’s instructions using Maxima SYBR Green/Rox (Thermo Scientific).

    Concentration Assay:

    Article Title: MicroRNA-148b and microRNA-152 reactivate tumor suppressor genes through suppression of DNA methyltransferase-1 gene in pancreatic cancer cell lines
    Article Snippet: .. RNA concentration was determined by spectrophotometer (IMPLEN,) and RNA quality was evaluated by determining the A260/A280 ratio. cDNA was synthesized by M-MuLV reverse transcriptase enzyme with 1 µg RNA using the cDNA synthesis kit (Fermentas, EP0351) according to the manufacturer’s recommendations. ..

    Incubation:

    Article Title: Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway
    Article Snippet: .. 1 mM), RNAse inhibitor (20 U, Fermentas) and RNAse free H2 O were added and the mixture incubated at 25°C for 5 min, after which the M-MuLV reverse transcriptase enzyme (20 U, Fermentas) was added and the mixture incubated for 60 min at 37°C. .. PCR reactions for CecA1 and Act5C were carried out according to manufacturer's instructions using the synthesized cDNAs as templates.

    Spectrophotometry:

    Article Title: MicroRNA-148b and microRNA-152 reactivate tumor suppressor genes through suppression of DNA methyltransferase-1 gene in pancreatic cancer cell lines
    Article Snippet: .. RNA concentration was determined by spectrophotometer (IMPLEN,) and RNA quality was evaluated by determining the A260/A280 ratio. cDNA was synthesized by M-MuLV reverse transcriptase enzyme with 1 µg RNA using the cDNA synthesis kit (Fermentas, EP0351) according to the manufacturer’s recommendations. ..

    Expressing:

    Article Title: Toll-like receptor-mediated, enhanced production of profibrotic TIMP-1 in monocytes from patients with systemic sclerosis: role of serum factors
    Article Snippet: .. RNA (200–750 ng) was treated with DNAse and reverse transcribed to cDNA with the use of random hexamers and the Moloney murine leukaemia virus reverse transcriptase enzyme (Invitrogen), according to the manufacturer's protocol. cDNA (20 ng), forward and reverse primer and probe in Taq ready mastermix was used for TIMP-1 expression analyses (see online supplementary table S1). .. MMP-1 expression was analysed using SYBR Green mix.

    Article Title: Beta-Catenin Activation Promotes Liver Regeneration after Acetaminophen-Induced Injury
    Article Snippet: .. Following DNase treatment mRNA was converted to cDNA using M-MuLV Reverse transcriptase enzyme (Invitrogen, Carlsbad, CA) in a reverse transcription (RT)-mastermix containing random primers, 5× RT buffer, dNTP mix, RiboLock, and M-MuLV Reverse transcriptase enzyme. β-Catenin message was estimated using the mouse TaqMan Gene Expression Assay (Assay ID-Mm00483033_m1, Applied Biosystems, Foster City, CA) in ABI PRISM 7000 machine according to manufacturers protocol. β-Actin was used as internal control. ..

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    Thermo Fisher m mlv rt
    M Mlv Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv rt/product/Thermo Fisher
    Average 99 stars, based on 1577 article reviews
    Price from $9.99 to $1999.99
    m mlv rt - by Bioz Stars, 2020-05
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