chromatographic column  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Aldrich chromatography column
    Description:

    Catalog Number:
    z547875
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore chromatographic column
    Aldrich chromatography column

    https://www.bioz.com/result/chromatographic column/product/Millipore
    Average 99 stars, based on 395 article reviews
    Price from $9.99 to $1999.99
    chromatographic column - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Genetic Regulation of the yefM-yoeB Toxin-Antitoxin Locus of Streptococcus pneumoniae ▿ ▿ † ▿ † §
    Article Snippet: .. The crude lysate was then loaded into a chromatography column packed with HIS-Select nickel affinity gel (Sigma) with a flow rate of 45 ml/h. .. HIS-Select nickel affinity gel is an immobilized metal-ion affinity chromatography product which is a quadridentate chelate on 6% beaded agarose charged with nickel and is selective for recombinant proteins with histidine tags and exhibits low nonspecific binding of other proteins.

    Chromatography:

    Article Title: Biochemical Analysis of Protein SUMOylation
    Article Snippet: .. Luria-Bertani (LB) broth (Sigma), (20g LB powder/1 L H2 O), autoclaved 500 mg/ml Kanamycin stock solution (Fisher Scientific), 0.22 um filter-sterilized 100 mg/ml Ampicillin stock solution (Fisher Scientific), 0.22 um filter-sterilized 50 mg/ml Chloroamphenicol stock solution (Fisher Scientific) in ethanol, 0.22 um filter-sterilized 1 M Isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientifics) Milli-Q-water Shaker Incubator (Thermo-Electron Corp.) Ultracentrifuge (Beckman Avanti J-25I) Spectramax M5 (Molecular Devices) BugBuster 10X protein extraction reagent (Novagen) Protease inhibitor cocktail (Roche) 2X Binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl, 10 mM Imidazole, and 0.04% NaN3 ) 2X GST binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl and 0.04% NaN3 ) Benzonase (Novagen) 50 mM Phenylmethylsulfonyl fluoride (PMSF) Dithiothreitol (DTT) (Sigma) Chromatography column (10x100 mm) (Sigma) Column stand with clamp Ni-NTA resin (Qiagen) Glutathione agarose resin (Amersham Biosciences) Reduced glutathione (Sigma) Wash buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 20 mM Imidazole) Elution buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 300 mM Imidazole) 1X GST wash buffer (50 mM Tris, pH 7.5, 200 mM NaCl, 1 mM DTT) GST elution buffer (50 mM Tris-HCl, pH 7.5, 1 mM DTT, 20 mM Glutathione reduced) Dialysis buffer (50 mM Tris-HCl, pH 7.5, 5 mM DTT) Phosphate buffered saline (PBS) 15 ml 10kD and 30kD Centricon (Millipore) Cold room 2X SDS sample buffer with DTT 4–12% Bis-Tris gradient gel (Invitrogen) NuPAGE MOPS SDS running buffer (Invitrogen) SDS-PAGE apparatus (Invitrogen) SimplyBlue Stain (Invitrogen) 1.5 ml Sterile microcentrifuge tubes Bradford reagent (Bio-rad) Bradford reagent (Bio-rad) .. 1 Culture a single colony of freshly transformed cells in 25 ml of LB broth containing 0.5 mg/ml kanamycin and 0.05 mg/ml chloroamphenicol at 225 rpm for 12 hrs at 37 ºC. (For E1: LB broth contains 0.5 mg/ml kanamycin, 0.05 mg/ml chloroamphenicol and 0.1 mg/ml ampicillin).

    Article Title: Genetic Regulation of the yefM-yoeB Toxin-Antitoxin Locus of Streptococcus pneumoniae ▿ ▿ † ▿ † §
    Article Snippet: .. The crude lysate was then loaded into a chromatography column packed with HIS-Select nickel affinity gel (Sigma) with a flow rate of 45 ml/h. .. HIS-Select nickel affinity gel is an immobilized metal-ion affinity chromatography product which is a quadridentate chelate on 6% beaded agarose charged with nickel and is selective for recombinant proteins with histidine tags and exhibits low nonspecific binding of other proteins.

    Protease Inhibitor:

    Article Title: Biochemical Analysis of Protein SUMOylation
    Article Snippet: .. Luria-Bertani (LB) broth (Sigma), (20g LB powder/1 L H2 O), autoclaved 500 mg/ml Kanamycin stock solution (Fisher Scientific), 0.22 um filter-sterilized 100 mg/ml Ampicillin stock solution (Fisher Scientific), 0.22 um filter-sterilized 50 mg/ml Chloroamphenicol stock solution (Fisher Scientific) in ethanol, 0.22 um filter-sterilized 1 M Isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientifics) Milli-Q-water Shaker Incubator (Thermo-Electron Corp.) Ultracentrifuge (Beckman Avanti J-25I) Spectramax M5 (Molecular Devices) BugBuster 10X protein extraction reagent (Novagen) Protease inhibitor cocktail (Roche) 2X Binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl, 10 mM Imidazole, and 0.04% NaN3 ) 2X GST binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl and 0.04% NaN3 ) Benzonase (Novagen) 50 mM Phenylmethylsulfonyl fluoride (PMSF) Dithiothreitol (DTT) (Sigma) Chromatography column (10x100 mm) (Sigma) Column stand with clamp Ni-NTA resin (Qiagen) Glutathione agarose resin (Amersham Biosciences) Reduced glutathione (Sigma) Wash buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 20 mM Imidazole) Elution buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 300 mM Imidazole) 1X GST wash buffer (50 mM Tris, pH 7.5, 200 mM NaCl, 1 mM DTT) GST elution buffer (50 mM Tris-HCl, pH 7.5, 1 mM DTT, 20 mM Glutathione reduced) Dialysis buffer (50 mM Tris-HCl, pH 7.5, 5 mM DTT) Phosphate buffered saline (PBS) 15 ml 10kD and 30kD Centricon (Millipore) Cold room 2X SDS sample buffer with DTT 4–12% Bis-Tris gradient gel (Invitrogen) NuPAGE MOPS SDS running buffer (Invitrogen) SDS-PAGE apparatus (Invitrogen) SimplyBlue Stain (Invitrogen) 1.5 ml Sterile microcentrifuge tubes Bradford reagent (Bio-rad) Bradford reagent (Bio-rad) .. 1 Culture a single colony of freshly transformed cells in 25 ml of LB broth containing 0.5 mg/ml kanamycin and 0.05 mg/ml chloroamphenicol at 225 rpm for 12 hrs at 37 ºC. (For E1: LB broth contains 0.5 mg/ml kanamycin, 0.05 mg/ml chloroamphenicol and 0.1 mg/ml ampicillin).

    Protein Extraction:

    Article Title: Biochemical Analysis of Protein SUMOylation
    Article Snippet: .. Luria-Bertani (LB) broth (Sigma), (20g LB powder/1 L H2 O), autoclaved 500 mg/ml Kanamycin stock solution (Fisher Scientific), 0.22 um filter-sterilized 100 mg/ml Ampicillin stock solution (Fisher Scientific), 0.22 um filter-sterilized 50 mg/ml Chloroamphenicol stock solution (Fisher Scientific) in ethanol, 0.22 um filter-sterilized 1 M Isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientifics) Milli-Q-water Shaker Incubator (Thermo-Electron Corp.) Ultracentrifuge (Beckman Avanti J-25I) Spectramax M5 (Molecular Devices) BugBuster 10X protein extraction reagent (Novagen) Protease inhibitor cocktail (Roche) 2X Binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl, 10 mM Imidazole, and 0.04% NaN3 ) 2X GST binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl and 0.04% NaN3 ) Benzonase (Novagen) 50 mM Phenylmethylsulfonyl fluoride (PMSF) Dithiothreitol (DTT) (Sigma) Chromatography column (10x100 mm) (Sigma) Column stand with clamp Ni-NTA resin (Qiagen) Glutathione agarose resin (Amersham Biosciences) Reduced glutathione (Sigma) Wash buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 20 mM Imidazole) Elution buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 300 mM Imidazole) 1X GST wash buffer (50 mM Tris, pH 7.5, 200 mM NaCl, 1 mM DTT) GST elution buffer (50 mM Tris-HCl, pH 7.5, 1 mM DTT, 20 mM Glutathione reduced) Dialysis buffer (50 mM Tris-HCl, pH 7.5, 5 mM DTT) Phosphate buffered saline (PBS) 15 ml 10kD and 30kD Centricon (Millipore) Cold room 2X SDS sample buffer with DTT 4–12% Bis-Tris gradient gel (Invitrogen) NuPAGE MOPS SDS running buffer (Invitrogen) SDS-PAGE apparatus (Invitrogen) SimplyBlue Stain (Invitrogen) 1.5 ml Sterile microcentrifuge tubes Bradford reagent (Bio-rad) Bradford reagent (Bio-rad) .. 1 Culture a single colony of freshly transformed cells in 25 ml of LB broth containing 0.5 mg/ml kanamycin and 0.05 mg/ml chloroamphenicol at 225 rpm for 12 hrs at 37 ºC. (For E1: LB broth contains 0.5 mg/ml kanamycin, 0.05 mg/ml chloroamphenicol and 0.1 mg/ml ampicillin).

    Staining:

    Article Title: Biochemical Analysis of Protein SUMOylation
    Article Snippet: .. Luria-Bertani (LB) broth (Sigma), (20g LB powder/1 L H2 O), autoclaved 500 mg/ml Kanamycin stock solution (Fisher Scientific), 0.22 um filter-sterilized 100 mg/ml Ampicillin stock solution (Fisher Scientific), 0.22 um filter-sterilized 50 mg/ml Chloroamphenicol stock solution (Fisher Scientific) in ethanol, 0.22 um filter-sterilized 1 M Isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientifics) Milli-Q-water Shaker Incubator (Thermo-Electron Corp.) Ultracentrifuge (Beckman Avanti J-25I) Spectramax M5 (Molecular Devices) BugBuster 10X protein extraction reagent (Novagen) Protease inhibitor cocktail (Roche) 2X Binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl, 10 mM Imidazole, and 0.04% NaN3 ) 2X GST binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl and 0.04% NaN3 ) Benzonase (Novagen) 50 mM Phenylmethylsulfonyl fluoride (PMSF) Dithiothreitol (DTT) (Sigma) Chromatography column (10x100 mm) (Sigma) Column stand with clamp Ni-NTA resin (Qiagen) Glutathione agarose resin (Amersham Biosciences) Reduced glutathione (Sigma) Wash buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 20 mM Imidazole) Elution buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 300 mM Imidazole) 1X GST wash buffer (50 mM Tris, pH 7.5, 200 mM NaCl, 1 mM DTT) GST elution buffer (50 mM Tris-HCl, pH 7.5, 1 mM DTT, 20 mM Glutathione reduced) Dialysis buffer (50 mM Tris-HCl, pH 7.5, 5 mM DTT) Phosphate buffered saline (PBS) 15 ml 10kD and 30kD Centricon (Millipore) Cold room 2X SDS sample buffer with DTT 4–12% Bis-Tris gradient gel (Invitrogen) NuPAGE MOPS SDS running buffer (Invitrogen) SDS-PAGE apparatus (Invitrogen) SimplyBlue Stain (Invitrogen) 1.5 ml Sterile microcentrifuge tubes Bradford reagent (Bio-rad) Bradford reagent (Bio-rad) .. 1 Culture a single colony of freshly transformed cells in 25 ml of LB broth containing 0.5 mg/ml kanamycin and 0.05 mg/ml chloroamphenicol at 225 rpm for 12 hrs at 37 ºC. (For E1: LB broth contains 0.5 mg/ml kanamycin, 0.05 mg/ml chloroamphenicol and 0.1 mg/ml ampicillin).

    Binding Assay:

    Article Title: Biochemical Analysis of Protein SUMOylation
    Article Snippet: .. Luria-Bertani (LB) broth (Sigma), (20g LB powder/1 L H2 O), autoclaved 500 mg/ml Kanamycin stock solution (Fisher Scientific), 0.22 um filter-sterilized 100 mg/ml Ampicillin stock solution (Fisher Scientific), 0.22 um filter-sterilized 50 mg/ml Chloroamphenicol stock solution (Fisher Scientific) in ethanol, 0.22 um filter-sterilized 1 M Isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientifics) Milli-Q-water Shaker Incubator (Thermo-Electron Corp.) Ultracentrifuge (Beckman Avanti J-25I) Spectramax M5 (Molecular Devices) BugBuster 10X protein extraction reagent (Novagen) Protease inhibitor cocktail (Roche) 2X Binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl, 10 mM Imidazole, and 0.04% NaN3 ) 2X GST binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl and 0.04% NaN3 ) Benzonase (Novagen) 50 mM Phenylmethylsulfonyl fluoride (PMSF) Dithiothreitol (DTT) (Sigma) Chromatography column (10x100 mm) (Sigma) Column stand with clamp Ni-NTA resin (Qiagen) Glutathione agarose resin (Amersham Biosciences) Reduced glutathione (Sigma) Wash buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 20 mM Imidazole) Elution buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 300 mM Imidazole) 1X GST wash buffer (50 mM Tris, pH 7.5, 200 mM NaCl, 1 mM DTT) GST elution buffer (50 mM Tris-HCl, pH 7.5, 1 mM DTT, 20 mM Glutathione reduced) Dialysis buffer (50 mM Tris-HCl, pH 7.5, 5 mM DTT) Phosphate buffered saline (PBS) 15 ml 10kD and 30kD Centricon (Millipore) Cold room 2X SDS sample buffer with DTT 4–12% Bis-Tris gradient gel (Invitrogen) NuPAGE MOPS SDS running buffer (Invitrogen) SDS-PAGE apparatus (Invitrogen) SimplyBlue Stain (Invitrogen) 1.5 ml Sterile microcentrifuge tubes Bradford reagent (Bio-rad) Bradford reagent (Bio-rad) .. 1 Culture a single colony of freshly transformed cells in 25 ml of LB broth containing 0.5 mg/ml kanamycin and 0.05 mg/ml chloroamphenicol at 225 rpm for 12 hrs at 37 ºC. (For E1: LB broth contains 0.5 mg/ml kanamycin, 0.05 mg/ml chloroamphenicol and 0.1 mg/ml ampicillin).

    SDS Page:

    Article Title: Biochemical Analysis of Protein SUMOylation
    Article Snippet: .. Luria-Bertani (LB) broth (Sigma), (20g LB powder/1 L H2 O), autoclaved 500 mg/ml Kanamycin stock solution (Fisher Scientific), 0.22 um filter-sterilized 100 mg/ml Ampicillin stock solution (Fisher Scientific), 0.22 um filter-sterilized 50 mg/ml Chloroamphenicol stock solution (Fisher Scientific) in ethanol, 0.22 um filter-sterilized 1 M Isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientifics) Milli-Q-water Shaker Incubator (Thermo-Electron Corp.) Ultracentrifuge (Beckman Avanti J-25I) Spectramax M5 (Molecular Devices) BugBuster 10X protein extraction reagent (Novagen) Protease inhibitor cocktail (Roche) 2X Binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl, 10 mM Imidazole, and 0.04% NaN3 ) 2X GST binding buffer (100 mM Tris-HCl, pH 7.5, 1 M NaCl and 0.04% NaN3 ) Benzonase (Novagen) 50 mM Phenylmethylsulfonyl fluoride (PMSF) Dithiothreitol (DTT) (Sigma) Chromatography column (10x100 mm) (Sigma) Column stand with clamp Ni-NTA resin (Qiagen) Glutathione agarose resin (Amersham Biosciences) Reduced glutathione (Sigma) Wash buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 20 mM Imidazole) Elution buffer (50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 300 mM Imidazole) 1X GST wash buffer (50 mM Tris, pH 7.5, 200 mM NaCl, 1 mM DTT) GST elution buffer (50 mM Tris-HCl, pH 7.5, 1 mM DTT, 20 mM Glutathione reduced) Dialysis buffer (50 mM Tris-HCl, pH 7.5, 5 mM DTT) Phosphate buffered saline (PBS) 15 ml 10kD and 30kD Centricon (Millipore) Cold room 2X SDS sample buffer with DTT 4–12% Bis-Tris gradient gel (Invitrogen) NuPAGE MOPS SDS running buffer (Invitrogen) SDS-PAGE apparatus (Invitrogen) SimplyBlue Stain (Invitrogen) 1.5 ml Sterile microcentrifuge tubes Bradford reagent (Bio-rad) Bradford reagent (Bio-rad) .. 1 Culture a single colony of freshly transformed cells in 25 ml of LB broth containing 0.5 mg/ml kanamycin and 0.05 mg/ml chloroamphenicol at 225 rpm for 12 hrs at 37 ºC. (For E1: LB broth contains 0.5 mg/ml kanamycin, 0.05 mg/ml chloroamphenicol and 0.1 mg/ml ampicillin).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Millipore molecule weight cut off membrane
    Molecule Weight Cut Off Membrane, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecule weight cut off membrane/product/Millipore
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    molecule weight cut off membrane - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    85
    Millipore molecular weight cut off amiconultra
    Molecular Weight Cut Off Amiconultra, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight cut off amiconultra/product/Millipore
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    molecular weight cut off amiconultra - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    89
    Millipore molecular weight cut off filter
    Molecular Weight Cut Off Filter, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecular weight cut off filter/product/Millipore
    Average 89 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    molecular weight cut off filter - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results