Journal: Journal of Extracellular Vesicles

Article Title: Targeted Delivery of GLUT1 Inhibitor via Macrophage Nanovesicles for Pulmonary Arterial Hypertension Therapy

doi: 10.1002/jev2.70294

Figure Lengend Snippet: Mac@WZB117 reverses macrophage polarization imbalance in lung tissues of PAH mice. (A) Flow cytometry gating strategy: after FSC/SSC selection, F4/80 + was used as a macrophage marker, with CD86 and CD206 labelling M1 and M2 phenotypes, respectively. (B) The proportion of M1 macrophages decreased significantly to 9.2% ± 1.5%, then rose to 28.7% ± 2.6% after Mac@WZB117 intervention ( p < 0.01, n = 5). (C) The proportion of F4/80 + CD206 + M2 macrophages increased to 45.3% ± 3.7% in the PAH + NS group and decreased to 20.1% ± 2.2% after Mac@WZB117 treatment ( p < 0.01). Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: An in vitro M1 polarization model was established using the murine macrophage cell line RAW264.7 (ATCC, TIB‐71).

Techniques: Flow Cytometry, Selection, Marker

Journal: Journal of Extracellular Vesicles

Article Title: Targeted Delivery of GLUT1 Inhibitor via Macrophage Nanovesicles for Pulmonary Arterial Hypertension Therapy

doi: 10.1002/jev2.70294

Figure Lengend Snippet: Glycolytic reactivation partially attenuates the therapeutic effects of Mac@WZB117. (A) Changes in macrophage phenotype proportions, FACS results showed an increased proportion of M2 macrophages and a decreased proportion of M1 macrophages in the rescue group, indicating reversal of macrophage polarization. (B) qPCR data showed iNOS expression was upregulated, while ARG1 and Mrc1 expression was downregulated, confirming that phenotype remodelling depends on glycolysis inhibition. (C) Haemodynamic parameters (RVSP and mPAP) were significantly elevated after rescue treatment, approaching levels observed in the PAH model. (D) Representative histological staining images and quantitative analysis showed that vascular WT% and α‐SMA‐positive area were markedly increased in the rescue group, suggesting partial reversal of pulmonary vascular structural remodelling. Data are presented as mean ± SD. Statistical significance is indicated by * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: An in vitro M1 polarization model was established using the murine macrophage cell line RAW264.7 (ATCC, TIB‐71).

Techniques: Expressing, Inhibition, Staining

Journal: Current Issues in Molecular Biology

Article Title: Development of a Novel Immunoprotective Culture System for Parathyroid Allografts: Utilizing Static Magnetic Fields to Modulate Lymphocyte Migration

doi: 10.3390/cimb48040388

Figure Lengend Snippet: Qualitative Transcriptional Profiling of Jurkat T-Lymphocyte-Specific Biomarkers via Semi-Quantitative RT-PCR. ( A – C ) Electrophoretic analysis of IL-1α, IL-1β, and IL-2 genes in Jurkat cell populations at 24 and 72 h for ( A ) groups 5–6 (lymphocytes only), ( B ) groups 7–8 (non-encapsulated co-culture), and ( C ) groups 9–10 (encapsulated co-culture). Molecular sizes are expressed in base pairs (bp), representing the specific PCR amplicon lengths for each primer set. All visible bands matched the predicted nucleotide lengths for IL-1α (147 bp) and β-actin (142 bp), confirming target-specific gene amplification across all Jurkat-containing groups.

Article Snippet: The Jurkat cell line (ATCC, #TIB-152TM) served as the lymphocyte model. Jurkat cells were cultured in complete RPMI-1640 (1X, Gibco, Waltham, MA, USA) medium supplemented with 10% ( v / v ) FBS and 1% ( v / v ) P/S.

Techniques: Quantitative RT-PCR, Co-Culture Assay, Amplification

Journal: Current Issues in Molecular Biology

Article Title: Development of a Novel Immunoprotective Culture System for Parathyroid Allografts: Utilizing Static Magnetic Fields to Modulate Lymphocyte Migration

doi: 10.3390/cimb48040388

Figure Lengend Snippet: Spatiotemporal Analysis of Jurkat Cell Migration Dynamics via Live-Cell Imaging. ( A , B ) Representative bright-field micrographs of Group 9 (encapsulated parathyroid cells co-cultured with Jurkat lymphocytes in the absence of SMF) at 24 ( A , C ) and 72 ( B , D ) h, illustrating a randomized lymphocyte distribution at the capsule interface. ( C , D ) Corresponding images of Group 10 (SMF-exposed) demonstrating directional magnetophoretic migration and the emergence of distinct lymphocyte-depleted zones surrounding the alginate capsule. Magnification: 2.5×. Black arrows denote the sodium alginate microcapsule boundary; black arrowheads indicate Jurkat cell populations. Note: Dynamic mobilization and altered spatial distribution vectors are further documented in .

Article Snippet: The Jurkat cell line (ATCC, #TIB-152TM) served as the lymphocyte model. Jurkat cells were cultured in complete RPMI-1640 (1X, Gibco, Waltham, MA, USA) medium supplemented with 10% ( v / v ) FBS and 1% ( v / v ) P/S.

Techniques: Migration, Live Cell Imaging, Cell Culture

Journal: Current Issues in Molecular Biology

Article Title: Development of a Novel Immunoprotective Culture System for Parathyroid Allografts: Utilizing Static Magnetic Fields to Modulate Lymphocyte Migration

doi: 10.3390/cimb48040388

Figure Lengend Snippet: Comparative Evaluation of Lymphocyte Positioning in Non-Encapsulated Co-Culture Systems. ( A , B ) Micrographs of Group 7 (non-encapsulated parathyroid cells + Jurkat cells; SMF-) and ( C , D ) Group 8 (non-encapsulated parathyroid cells + Jurkat cells; SMF+) after 24 ( A , C ) and 72 ( B , D ) hours of incubation. In the absence of an alginate physical barrier, no significant directional migration or separation was observed regardless of magnetic field application, highlighting the essential synergistic role of the combinatorial encapsulation–SMF approach for immune cell modulation. Magnification: 10×. Arrows indicate non-encapsulated parathyroid cell clusters; arrowheads denote Jurkat cell distribution.

Article Snippet: The Jurkat cell line (ATCC, #TIB-152TM) served as the lymphocyte model. Jurkat cells were cultured in complete RPMI-1640 (1X, Gibco, Waltham, MA, USA) medium supplemented with 10% ( v / v ) FBS and 1% ( v / v ) P/S.

Techniques: Co-Culture Assay, Incubation, Migration, Encapsulation