Structured Review

Mobitec mobispin s 400 spin columns
Mobispin S 400 Spin Columns, supplied by Mobitec, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mobispin s 400 spin columns/product/Mobitec
Average 85 stars, based on 6 article reviews
Price from $9.99 to $1999.99
mobispin s 400 spin columns - by Bioz Stars, 2020-01
85/100 stars

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Related Articles

RNA Extraction:

Article Title: Divergent ancestral lineages of newfound hantaviruses harbored by phylogenetically related crocidurine shrew species in Korea
Article Snippet: Paragraph title: RNA extraction, cDNA synthesis and RT-PCR amplification ... PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Molecular Phylogeny of Hantaviruses Harbored by Insectivorous Bats in C?te d'Ivoire and Vietnam
Article Snippet: Genome Detection and Sequencing Total RNA extraction from tissues, using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA, USA), and cDNA synthesis, using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with random hexamers, were performed as described previously [ , , ]. .. PCR products, separated using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) [ , ].

Amplification:

Article Title: Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea)
Article Snippet: The 5′– and 3′–termini of each segment were amplified using the 3′–Full RACE Core Set (Takara Bio Inc., Otsu, Japan). .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA) .

Article Title: Divergent ancestral lineages of newfound hantaviruses harbored by phylogenetically related crocidurine shrew species in Korea
Article Snippet: Paragraph title: RNA extraction, cDNA synthesis and RT-PCR amplification ... PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Synthesized:

Article Title: Divergent ancestral lineages of newfound hantaviruses harbored by phylogenetically related crocidurine shrew species in Korea
Article Snippet: Total RNA was extracted from 20–50 mg of each tissue, using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA). cDNA, synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen), were analyzed for hantavirus RNA by RT-PCR, using primers designed from highly conserved regions of hantavirus genomes ( , , ; , , ; , , ). .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Genetic variants of Cao Bang hantavirus in the Chinese mole shrew (Anourosorex squamipes) and Taiwanese mole shrew (Anourosorex yamashinai)
Article Snippet: Total RNA was extracted from tissues using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA), and cDNA was synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with a highly conserved primer and/or random hexamers by two-step RT-PCR cycles. .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Dahonggou Creek virus, a divergent lineage of hantavirus harbored by the long-tailed mole (Scaptonyx fusicaudus)
Article Snippet: Briefly, total RNA was extracted from tissues using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA), and cDNA was synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with a highly conserved primer and/or random hexamers by two-step RT-PCR cycles. .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Polymerase Chain Reaction:

Article Title: Molecular Phylogenetic Analysis of Orientia tsutsugamushi Based on the groES and groEL Genes
Article Snippet: .. PCR products were separated with MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. Sequences were processed using Genetyx version 9.1 (Genetyx Corporation, Tokyo, Japan) and aligned with CLUSTALW and CLUSTALW2 (Thompson et al. ) using publicly available O. tsutsugamushi sequences.

Article Title: Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea)
Article Snippet: .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA) . ..

Article Title: Divergent ancestral lineages of newfound hantaviruses harbored by phylogenetically related crocidurine shrew species in Korea
Article Snippet: .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. Sequences were processed using the Genetyx version 9 software (Genetyx Corporation, Tokyo, Japan) and aligned using Clustal W and Clustal W2 ( ) with publicly available hantavirus sequences.

Article Title: Genetic variants of Cao Bang hantavirus in the Chinese mole shrew (Anourosorex squamipes) and Taiwanese mole shrew (Anourosorex yamashinai)
Article Snippet: .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. Phylogenetic trees were generated by maximum likelihood and Bayesian methods, implemented in PAUP* (Phylogenetic Analysis Using Parsimony, 4.0b10) , RAxML Blackbox webserver ( ) and MrBayes 3.1 , under the best-fit GTR+I+Γ model of evolution selected by hierarchical likelihood-ratio test in MrModeltest v2.3 ( ) and jModelTest version 0.1 ( ).

Article Title: Molecular Phylogeny of Hantaviruses Harbored by Insectivorous Bats in C?te d'Ivoire and Vietnam
Article Snippet: .. PCR products, separated using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) [ , ]. ..

Article Title: Dahonggou Creek virus, a divergent lineage of hantavirus harbored by the long-tailed mole (Scaptonyx fusicaudus)
Article Snippet: .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. Phylogenetic trees were generated using maximum likelihood and Bayesian methods, implemented in the RAxML Blackbox webserver [ ] and MrBayes 3.1 [ ], under the best-fit GTR+I+Γ model of evolution selected by hierarchical likelihood-ratio test in MrModeltest v2.3 [ ] and jModelTest version 0.1 [ ].

Size-exclusion Chromatography:

Article Title: Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea)
Article Snippet: Initial denaturation at 94 °C for 2 min was followed by two cycles each of denaturation at 94 °C for 30 sec, two-degree step-down annealing from 46 °C to 38 °C for 40 sec, and elongation at 72 °C for 1 min, then 30 cycles of denaturation at 94 °C for 30 sec, annealing at 42 °C for 40 sec, and elongation at 72 °C for 1 min, in a GeneAmp PCR 9700 thermal cycler (Perkin-Elmer, Waltham, MA) . .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA) .

Article Title: Divergent ancestral lineages of newfound hantaviruses harbored by phylogenetically related crocidurine shrew species in Korea
Article Snippet: Initial denaturation at 94°C for 2 min was followed by two cycles each of denaturation at 94°C for 30 sec, two-degree step-down annealing from 46°C to 38°C for 40 sec, and elongation at 72°C for 1 min, then 30 cycles of denaturation at 94°C for 30 sec, annealing at 42°C for 40 sec, and elongation at 72°C for 1 min, in a GeneAmp PCR 9700 thermal cycler (Perkin-Elmer, Waltham, MA) ( ). .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Genetic variants of Cao Bang hantavirus in the Chinese mole shrew (Anourosorex squamipes) and Taiwanese mole shrew (Anourosorex yamashinai)
Article Snippet: Initial denaturation at 94°C for 2 min was followed by two cycles each of denaturation at 94°C for 30 sec, two-degree step-down annealing from 46°C to 38°C for 40 sec, and elongation at 72°C for 1 min, then 30 cycles of denaturation at 94°C for 30 sec, annealing at 42°C for 40 sec, and elongation at 72°C for 1 min, in a GeneAmp PCR 9700 thermal cycler (Perkin-Elmer, Waltham, MA). .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Purification:

Article Title: Divergent ancestral lineages of newfound hantaviruses harbored by phylogenetically related crocidurine shrew species in Korea
Article Snippet: Total RNA was extracted from 20–50 mg of each tissue, using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA). cDNA, synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen), were analyzed for hantavirus RNA by RT-PCR, using primers designed from highly conserved regions of hantavirus genomes ( , , ; , , ; , , ). .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Genetic variants of Cao Bang hantavirus in the Chinese mole shrew (Anourosorex squamipes) and Taiwanese mole shrew (Anourosorex yamashinai)
Article Snippet: Total RNA was extracted from tissues using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA), and cDNA was synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with a highly conserved primer and/or random hexamers by two-step RT-PCR cycles. .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Molecular Phylogeny of Hantaviruses Harbored by Insectivorous Bats in C?te d'Ivoire and Vietnam
Article Snippet: Genome Detection and Sequencing Total RNA extraction from tissues, using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA, USA), and cDNA synthesis, using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with random hexamers, were performed as described previously [ , , ]. .. PCR products, separated using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) [ , ].

Article Title: Dahonggou Creek virus, a divergent lineage of hantavirus harbored by the long-tailed mole (Scaptonyx fusicaudus)
Article Snippet: Briefly, total RNA was extracted from tissues using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA), and cDNA was synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with a highly conserved primer and/or random hexamers by two-step RT-PCR cycles. .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Sequencing:

Article Title: Isolation and partial characterization of a highly divergent lineage of hantavirus from the European mole (Talpa europaea)
Article Snippet: Paragraph title: Whole genome sequencing ... PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA) .

Article Title: Molecular Phylogeny of Hantaviruses Harbored by Insectivorous Bats in C?te d'Ivoire and Vietnam
Article Snippet: Paragraph title: 3.2. Genome Detection and Sequencing ... PCR products, separated using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) [ , ].

Generated:

Article Title: Molecular Phylogenetic Analysis of Orientia tsutsugamushi Based on the groES and groEL Genes
Article Snippet: PCR products were separated with MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. The phylogenetic analysis was conducted using Bayesian interference trees, which were generated with Bayesian Metropolis Hastings Markov Chain Monte Carlo (MCMC) tree-sampling methods implemented in MrBayes (Ronquist and Huelsenbeck ) using a GTR+I+Γ model of evolution, i.e ., the hierarchical likelihood-ratio test (hLRT) in MrModeltest2.3 ( ) (Posada and Crandall ) with partitioning based on codon positions.

Article Title: Genetic variants of Cao Bang hantavirus in the Chinese mole shrew (Anourosorex squamipes) and Taiwanese mole shrew (Anourosorex yamashinai)
Article Snippet: PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. Phylogenetic trees were generated by maximum likelihood and Bayesian methods, implemented in PAUP* (Phylogenetic Analysis Using Parsimony, 4.0b10) , RAxML Blackbox webserver ( ) and MrBayes 3.1 , under the best-fit GTR+I+Γ model of evolution selected by hierarchical likelihood-ratio test in MrModeltest v2.3 ( ) and jModelTest version 0.1 ( ).

Article Title: Dahonggou Creek virus, a divergent lineage of hantavirus harbored by the long-tailed mole (Scaptonyx fusicaudus)
Article Snippet: PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. Phylogenetic trees were generated using maximum likelihood and Bayesian methods, implemented in the RAxML Blackbox webserver [ ] and MrBayes 3.1 [ ], under the best-fit GTR+I+Γ model of evolution selected by hierarchical likelihood-ratio test in MrModeltest v2.3 [ ] and jModelTest version 0.1 [ ].

Reverse Transcription Polymerase Chain Reaction:

Article Title: Divergent ancestral lineages of newfound hantaviruses harbored by phylogenetically related crocidurine shrew species in Korea
Article Snippet: Paragraph title: RNA extraction, cDNA synthesis and RT-PCR amplification ... PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Genetic variants of Cao Bang hantavirus in the Chinese mole shrew (Anourosorex squamipes) and Taiwanese mole shrew (Anourosorex yamashinai)
Article Snippet: Total RNA was extracted from tissues using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA), and cDNA was synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with a highly conserved primer and/or random hexamers by two-step RT-PCR cycles. .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

Article Title: Dahonggou Creek virus, a divergent lineage of hantavirus harbored by the long-tailed mole (Scaptonyx fusicaudus)
Article Snippet: Briefly, total RNA was extracted from tissues using the PureLink Micro-to-Midi total RNA purification kit (Invitrogen, San Diego, CA), and cDNA was synthesized using the SuperScript III First-Strand Synthesis Systems (Invitrogen) with a highly conserved primer and/or random hexamers by two-step RT-PCR cycles. .. PCR products were separated, using MobiSpin S-400 spin columns (MoBiTec, Goettingen, Germany), and amplicons were sequenced directly using an ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA).

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    Mobitec mobispin s 400 spin columns
    Mobispin S 400 Spin Columns, supplied by Mobitec, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mobispin s 400 spin columns/product/Mobitec
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mobispin s 400 spin columns - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

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