mobile phase b  (Millipore)


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    Name:
    Mobile phase B
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    Catalog Number:
    85278
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    Structured Review

    Millipore mobile phase b
    Mobile phase B

    https://www.bioz.com/result/mobile phase b/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mobile phase b - by Bioz Stars, 2020-10
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: A comprehensive proteomic SWATH-MS workflow for profiling blood extracellular vesicles: a new avenue for glioma tumour surveillance
    Article Snippet: .. Mobile phase buffers were comprised of A: 0.1% (v/v) TFA (Sigma, Chromasolv HPLC grade, Cat.No. ..

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Transferring:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Blocking Assay:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Oligonucleotide Synthesis:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Incubation:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    other:

    Article Title: Regulatory control circuits for stabilizing long-term anabolic product formation in yeast
    Article Snippet: Mobile phase A consisted of 10 mM ammonium formate, pH 3 while mobile phase B consisted of acetonitrile.

    Article Title: Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity
    Article Snippet: The phase separation by addition of 500 µL hexane was repeated twice, with the final hexane layer transferred to a 2 mL glass vial with a glass insert.

    Article Title: Parkin coordinates mitochondrial lipid remodeling to execute mitophagy
    Article Snippet: Mobile phase A consisted of chloroform/methanol/aqueous ammonium hydroxide (800:195:5, v/v/v); mobile phase B consisted of; mobile phase C consisted of.

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  • 98
    Millipore mobile phase b
    Mobile Phase B, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mobile phase b/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mobile phase b - by Bioz Stars, 2020-10
    98/100 stars
      Buy from Supplier

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