mobile phase b  (Millipore)


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    Mobile phase B
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    85278
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    Millipore mobile phase b
    Mobile phase B

    https://www.bioz.com/result/mobile phase b/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mobile phase b - by Bioz Stars, 2020-02
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    Blocking Assay:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Incubation:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Stripping Membranes:

    Article Title: Tuning Pharmacokinetics to Improve Tumor Accumulation of a Prostate-Specific Membrane Antigen-Targeted Phototheranostic Agent
    Article Snippet: .. The strip was then placed into a capped test tube containing mobile phase prepared with 2 vol % EDTA (Sigma-Aldrich Co. LLC) and 10 vol % 0.1 M NH4 OAc in ddH2 O. ..

    Activity Assay:

    Article Title: Nerve Growth Factor Rapidly Induces Prolonged Acetylcholine Release from Cultured Basal Forebrain Neurons: Differentiation between Neuromodulatory and Neurotrophic Influences
    Article Snippet: The mobile phase, 0.2 m aqueous potassium phosphate buffer, pH 8.0, with 1 m m tetramethylammonium hydroxide (Sigma), was delivered at 0.4–0.45 ml/min by a dual piston pump (ESA 580, Chelmsford, MA) connected to a degasser (CMA 260, Stockholm, Sweden). .. ACh eluted at ∼4 min. Choline acetyltransferase activity .

    Article Title: Comparison of Transitional vs Surgical Menopause on Monoamine and Amino Acid levels in the Rat Brain
    Article Snippet: Mobile Phase B consisted of 0.1 M lithium acetate (Sigma-Aldrich, Inc.) in a solvent mixture of methanol (Avantor Performance Materials, Inc.), acetonitrile (Honeywell, Inc.) and isopropanol (Mallinckrodt Baker, Inc.) at the ratio of 80/10/10 (v/v/v). pH was adjusted to 6.5 using acetic acid (Sigma-Aldrich, Inc.). .. Turnover ratios (metabolite/monoamine) were calculated as a measure of activity.

    Mass Spectrometry:

    Article Title: Response to Fungal Dysbiosis by Gut Resident CX3CR1+ Mononuclear Phagocytes Aggravates Allergic Airway Disease
    Article Snippet: Mouse serum (10 uL) was mixed with mobile phase B (0.1 % formic acid (Sigma-Aldrich) in acetonitrile (Sigma-Aldrich), 100 μL) and internal standard solution (fluconazole-d4 (1.63 μM in acetonitrile) 100 μL, Cayman Chemical). .. A 1290 Infinity II HPLC system (Agilent Technologies) coupled with a Triple Quad LC/MS (Agilent 6495) was used for analyte separation and identification by tandem mass spectrometry.

    Article Title: Graphene Oxide/Polyethylene Glycol-Stick for Thin Film Microextraction of β-Blockers from Human Oral Fluid by Liquid Chromatography-Tandem Mass Spectrometry
    Article Snippet: Deionized water used for the mobile phase was produced by Milli-Q system (Millipore Co, Bedford, MA. .. Samples were analyzed using a triple-quadrupole mass spectrometer, Z-spray Quattro-micro (Waters, Manchester, UK) with an electrospray ionization source (ESI).

    High Performance Liquid Chromatography:

    Article Title: Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin
    Article Snippet: .. The details of the HPLC fluorometric analysis are as follows: HPLC column, J-Pak Symphonia C18 (5 µm, 4.6 × 150 mm; Jasco, Tokyo, Japan); mobile phase, 0.06 M heptafluorobutiric acid (HFBA, 98%; Sigma-Aldrich, St. Louis, MO, USA) /0.05 M ammonium acetate buffer (pH 5.0), 0.4 mL/min; reaction reagent, 4 M sodium hydroxide, 0.4 mL/min; detector, FP-2020 Plus (Jasco, Ex 365 nm, Em 510 nm, Gain ×1000); pump for mobile phase, PU-2080 Plus (Jasco, Tokyo, Japan); pump for reaction reagent, PU-2080 Plus (Jasco, Tokyo, Japan); reaction coil, i.d.0.5 mm × 200 cm (stainless, 120 °C in dry oven); integrator, Chromatocorder 21 (SIC, Tokyo, Japan); injection volume, 10 µL (overload injection). ..

    Article Title: Nerve Growth Factor Rapidly Induces Prolonged Acetylcholine Release from Cultured Basal Forebrain Neurons: Differentiation between Neuromodulatory and Neurotrophic Influences
    Article Snippet: ACh was assayed by HPLC with electrochemical detection in conjunction with an enzyme reactor. .. The mobile phase, 0.2 m aqueous potassium phosphate buffer, pH 8.0, with 1 m m tetramethylammonium hydroxide (Sigma), was delivered at 0.4–0.45 ml/min by a dual piston pump (ESA 580, Chelmsford, MA) connected to a degasser (CMA 260, Stockholm, Sweden).

    Article Title: Conditioned and Unconditioned Stimuli Increase Frontal Cortical and Hippocampal Acetylcholine Release: Effects of Novelty, Habituation, and Fear
    Article Snippet: ACh was assayed by HPLC-ECD in conjunction with an enzyme reactor ( ). .. The mobile phase passed directly through the enzyme reactor (10 × 2.1 mm) containing acetylcholinesterase (EC 3.1.1.7 ; Sigma, type VI-S) and choline oxidase (EC 1.1.3.17 ; Sigma).

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: Paragraph title: HPLC Chromatographic Conditions ... Chromatographic conditions were set as previously described., Briefly, 10 μL aliquots of each extracted sample were injected into the column and eluted with a mobile phase consisting of 10 mM KH2 PO4 /acetonitrile (3:1 v/v; EMD Millipore, AX0145P1) (pH 4.0 adjusted with 1 M phosphoric acid), at a flow rate of 500 μL/min.

    Article Title: Response to Fungal Dysbiosis by Gut Resident CX3CR1+ Mononuclear Phagocytes Aggravates Allergic Airway Disease
    Article Snippet: Mouse serum (10 uL) was mixed with mobile phase B (0.1 % formic acid (Sigma-Aldrich) in acetonitrile (Sigma-Aldrich), 100 μL) and internal standard solution (fluconazole-d4 (1.63 μM in acetonitrile) 100 μL, Cayman Chemical). .. A 1290 Infinity II HPLC system (Agilent Technologies) coupled with a Triple Quad LC/MS (Agilent 6495) was used for analyte separation and identification by tandem mass spectrometry.

    Article Title: Production Standard and Stability of Compounded Del Nido Cardioplegia Solution
    Article Snippet: An Agilent 1200 series high-performance liquid chromatography system (Agilent Technologies, Wilmington, Delaware) was used, consisting of a degasser, a binary pump, an injector/autosampler, a column heater, and a cooled thermostat controlled at 4°C autosampler compartment. .. Both were maintained to 38°C throughout the entire analysis, eluted with a mobile phase of 50% v/v pure milliQ water with 0.1% v/v formic acid CovaChem (Sigma-Aldrich, St Louis, Missouri), and 50% v/v methanol Lichrosolv (Sigma-Aldrich, St Louis, Missouri).

    Article Title: Comparison of Transitional vs Surgical Menopause on Monoamine and Amino Acid levels in the Rat Brain
    Article Snippet: Paragraph title: 2.7. Monoamine analysis by HPLC-CMEAS ... Mobile Phase B consisted of 0.1 M lithium acetate (Sigma-Aldrich, Inc.) in a solvent mixture of methanol (Avantor Performance Materials, Inc.), acetonitrile (Honeywell, Inc.) and isopropanol (Mallinckrodt Baker, Inc.) at the ratio of 80/10/10 (v/v/v). pH was adjusted to 6.5 using acetic acid (Sigma-Aldrich, Inc.).

    Article Title: New Pre-reacted Glass Containing Dental Composites (giomers) with Improved Fluoride Release and Biocompatibility
    Article Snippet: .. The mobile phase was a mixture of acetonitrile (A, HPLC grade) and water (B, Milipore ultrapure water), and a gradient was applied according to the following method: 0–15 min. linear gradient 50–80% A; 15–25 min. linear gradient 80–50% A. ..

    Article Title: Tuning Pharmacokinetics to Improve Tumor Accumulation of a Prostate-Specific Membrane Antigen-Targeted Phototheranostic Agent
    Article Snippet: The strip was then placed into a capped test tube containing mobile phase prepared with 2 vol % EDTA (Sigma-Aldrich Co. LLC) and 10 vol % 0.1 M NH4 OAc in ddH2 O. .. The developed iTLC strip was cut in thirds and the 64 Cu radioactivity assayed for the two top (free 64 Cu) and separately for a bottom piece (64 Cu-LC-Pyro) using a Wizard 1480 well-type automatic gamma counter (PerkinElmer Inc.; Shelton, CT, USA) and radiochemical purity was further evaluated by radio HPLC performed on a XBridge-C18 column (2.5 μ m, 4.6 mm × 50 mm) with UV detector and radioactivity detector Figure S. Further purification of 64 Cu-LC-Pyro was deemed unnecessary, due to the high molar ratio of LC-Pyro:64 CuCl2 (~1000:1) and its high radiochemical purity.

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Article Title: SYSTEMIC ADMINISTRATION OF LEPTIN DECREASES PLASMA CORTICOSTERONE LEVELS
    Article Snippet: Paragraph title: HPLC ... The mobile phase was made in pyrogen-free water and then filtered and degassed through a Milli-Q purification system (Millipore, Bedford, MA, USA) and pumped at a flow rate of 1.8 ml/min.

    Flow Cytometry:

    Article Title: Conditioned and Unconditioned Stimuli Increase Frontal Cortical and Hippocampal Acetylcholine Release: Effects of Novelty, Habituation, and Fear
    Article Snippet: The mobile phase passed directly through the enzyme reactor (10 × 2.1 mm) containing acetylcholinesterase (EC 3.1.1.7 ; Sigma, type VI-S) and choline oxidase (EC 1.1.3.17 ; Sigma). .. The mobile phase was an aqueous potassium phosphate buffer (1.9 m m K2 HPO4 , 0.2 m m tetramethyl ammonium hydroxide, pH 8) delivered at a constant flow of 0.4 ml/min by an HPLC pump (LKB 2150).

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: .. Chromatographic conditions were set as previously described., Briefly, 10 μL aliquots of each extracted sample were injected into the column and eluted with a mobile phase consisting of 10 mM KH2 PO4 /acetonitrile (3:1 v/v; EMD Millipore, AX0145P1) (pH 4.0 adjusted with 1 M phosphoric acid), at a flow rate of 500 μL/min. .. The analysis was performed using an Eicom 700 system (Eicom Corporation, Kyoto, Japan) consisting of a Shimatzu RF-20AX fluorescence detector (Shimadzu, Kyoto, Japan), an Eicom 700 Insight autosampler, and Envision integration software.

    Article Title: Production Standard and Stability of Compounded Del Nido Cardioplegia Solution
    Article Snippet: Both were maintained to 38°C throughout the entire analysis, eluted with a mobile phase of 50% v/v pure milliQ water with 0.1% v/v formic acid CovaChem (Sigma-Aldrich, St Louis, Missouri), and 50% v/v methanol Lichrosolv (Sigma-Aldrich, St Louis, Missouri). .. Both were maintained to 38°C throughout the entire analysis, eluted with a mobile phase of 50% v/v pure milliQ water with 0.1% v/v formic acid CovaChem (Sigma-Aldrich, St Louis, Missouri), and 50% v/v methanol Lichrosolv (Sigma-Aldrich, St Louis, Missouri).

    Article Title: Comparison of Transitional vs Surgical Menopause on Monoamine and Amino Acid levels in the Rat Brain
    Article Snippet: Mobile Phase B consisted of 0.1 M lithium acetate (Sigma-Aldrich, Inc.) in a solvent mixture of methanol (Avantor Performance Materials, Inc.), acetonitrile (Honeywell, Inc.) and isopropanol (Mallinckrodt Baker, Inc.) at the ratio of 80/10/10 (v/v/v). pH was adjusted to 6.5 using acetic acid (Sigma-Aldrich, Inc.). .. The mobile phase was filtered through a 0.2 μm nylon filter (Sartorius Stedim Biotech, Inc.), and delivered at a fixed flow rate of 0.6 mL/min.

    Article Title: SYSTEMIC ADMINISTRATION OF LEPTIN DECREASES PLASMA CORTICOSTERONE LEVELS
    Article Snippet: .. The mobile phase was made in pyrogen-free water and then filtered and degassed through a Milli-Q purification system (Millipore, Bedford, MA, USA) and pumped at a flow rate of 1.8 ml/min. ..

    Chromatography:

    Article Title: Tuning Pharmacokinetics to Improve Tumor Accumulation of a Prostate-Specific Membrane Antigen-Targeted Phototheranostic Agent
    Article Snippet: Briefly, a 1 cm × 8 cm strip of heat-activated glass microfiber chromatography paper (Aligent Technologies, USA) was spotted with 2 μ L of sample 1.5 cm from the bottom of the strip. .. The strip was then placed into a capped test tube containing mobile phase prepared with 2 vol % EDTA (Sigma-Aldrich Co. LLC) and 10 vol % 0.1 M NH4 OAc in ddH2 O.

    Oligonucleotide Synthesis:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Transferring:

    Article Title: Synthesis of Fluorescence Turn-On DNA Hybridization Probe using the DEAtC 2’-deoxycytidine Analogue
    Article Snippet: .. 5′- O -Dimethoxytrityl- N 2 -isobutyryl-2′-deoxyguanosine, 3′- O -succinyl-long chain alkylamino-CPG (LCAA-CPG) support (500 Å pore size, 43 μmol/g, Glen Research) Standard DNA phosphoramidites (dried over activated 3Å molecular sieves at least 16 hrs): N 6 -Benzoyl-2′-deoxyadenosine phosphoramidite (Bz-dA, ChemGenes) N 4 -Acetyl-2′-deoxycytidine phosphoramidite (Ac-dC, Glen Research) N 2 -Isobutyryl-2′-deoxyguanosine phosphoramidite (iBu-dG, ChemGenes) Thymidine phosphoramidite (T, Glen Research) Phosphoramidite 8 (see ) Reagents for solid-phase oligonucleotide synthesis: Activator solution: 1 H -tetrazole in anhydrous acetonitrile (Glen Research) Cap A: Acetic anhydride (Alfa Aesar):2,6-lutidine (Acros):THF (1:1:8) Cap B: 16% 1-methylimidazole (Alfa Aesar) in THF (v/v) Oxidizing solution: 0.03 M I2 (Alfa Aesar) in THF/pyridine/H2 O (75:20:2) Deblocking Mix: 3% trichloroacetic acid in CH2 Cl2 Anhydrous acetonitrile wash (CH3 CN, Glen Research) Conc. aq. ammonia (28 – 30%, Sigma-Aldrich) Acetic Acid (Fisher Scientific, HPLC grade) 3 M sodium acetate (EM Science) 5 M sodium perchlorate (GFS Chemicals, HPLC grade) Acetone (EMD, HPLC grade) Ethanol (200 Proof, Pharmco-Aaper) Mobile phase A: 0.05 M triethylammonium acetate (TEAA, Glen Research), pH 7 Mobile phase B: 25% H2 O in acetonitrile ZipTipC18 Pipette Tips (Millipore) Matrix solution for MALDI-MS: 10 mg 2,4,6-trihydroxyacetophenone (THAP, Matrix Scientific) in 1:1 MeCN:H2 O (1 mL), 230-mg ammonium citrate, dibasic (Chem Impex) in 10 mL H2 O, mix 8:1 THAP:ammonium citrate Expedite 8909 Nucleic Acid Synthesis System Empty synthesis column, 0.2 μmol scale (Glen Research) 1- and 2- mL plastic syringes 1.5 mL microtube with o-ring cap (Sarstedt) 55 °C incubation block (Eppendorf ThermoStat Plus) 1.7 mL microcentrifuge tubes (Sorenson Bioscience) Speedvac evaporator (Labconco refrigerated centrivap concentrator, Model 7310021, or equivalent) Thermo Fisher Vanquish UHPLC system equipped with: Hypersil Gold C18 column (5 μm, 4.6 × 150 mm, Thermo Fisher) Fraction Collector (Dionex UltiMate 3000) 1. ..

    Sequencing:

    Article Title: Conditioned and Unconditioned Stimuli Increase Frontal Cortical and Hippocampal Acetylcholine Release: Effects of Novelty, Habituation, and Fear
    Article Snippet: Each valve was electrically operated by a digital valve sequence programmer (Valco Instruments) automatically injecting the sample for HPLC-ECD analysis every 10 min. .. The mobile phase passed directly through the enzyme reactor (10 × 2.1 mm) containing acetylcholinesterase (EC 3.1.1.7 ; Sigma, type VI-S) and choline oxidase (EC 1.1.3.17 ; Sigma).

    Injection:

    Article Title: Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin
    Article Snippet: .. The details of the HPLC fluorometric analysis are as follows: HPLC column, J-Pak Symphonia C18 (5 µm, 4.6 × 150 mm; Jasco, Tokyo, Japan); mobile phase, 0.06 M heptafluorobutiric acid (HFBA, 98%; Sigma-Aldrich, St. Louis, MO, USA) /0.05 M ammonium acetate buffer (pH 5.0), 0.4 mL/min; reaction reagent, 4 M sodium hydroxide, 0.4 mL/min; detector, FP-2020 Plus (Jasco, Ex 365 nm, Em 510 nm, Gain ×1000); pump for mobile phase, PU-2080 Plus (Jasco, Tokyo, Japan); pump for reaction reagent, PU-2080 Plus (Jasco, Tokyo, Japan); reaction coil, i.d.0.5 mm × 200 cm (stainless, 120 °C in dry oven); integrator, Chromatocorder 21 (SIC, Tokyo, Japan); injection volume, 10 µL (overload injection). ..

    Article Title: Nerve Growth Factor Rapidly Induces Prolonged Acetylcholine Release from Cultured Basal Forebrain Neurons: Differentiation between Neuromodulatory and Neurotrophic Influences
    Article Snippet: Samples (100 μl) were injected manually via a 100 μl loop on a two-position valve (Valco, Houston, TX). .. The mobile phase, 0.2 m aqueous potassium phosphate buffer, pH 8.0, with 1 m m tetramethylammonium hydroxide (Sigma), was delivered at 0.4–0.45 ml/min by a dual piston pump (ESA 580, Chelmsford, MA) connected to a degasser (CMA 260, Stockholm, Sweden).

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: .. Chromatographic conditions were set as previously described., Briefly, 10 μL aliquots of each extracted sample were injected into the column and eluted with a mobile phase consisting of 10 mM KH2 PO4 /acetonitrile (3:1 v/v; EMD Millipore, AX0145P1) (pH 4.0 adjusted with 1 M phosphoric acid), at a flow rate of 500 μL/min. .. The analysis was performed using an Eicom 700 system (Eicom Corporation, Kyoto, Japan) consisting of a Shimatzu RF-20AX fluorescence detector (Shimadzu, Kyoto, Japan), an Eicom 700 Insight autosampler, and Envision integration software.

    Article Title: Production Standard and Stability of Compounded Del Nido Cardioplegia Solution
    Article Snippet: Both were maintained to 38°C throughout the entire analysis, eluted with a mobile phase of 50% v/v pure milliQ water with 0.1% v/v formic acid CovaChem (Sigma-Aldrich, St Louis, Missouri), and 50% v/v methanol Lichrosolv (Sigma-Aldrich, St Louis, Missouri). .. Both were maintained to 38°C throughout the entire analysis, eluted with a mobile phase of 50% v/v pure milliQ water with 0.1% v/v formic acid CovaChem (Sigma-Aldrich, St Louis, Missouri), and 50% v/v methanol Lichrosolv (Sigma-Aldrich, St Louis, Missouri).

    Article Title: Comparison of Transitional vs Surgical Menopause on Monoamine and Amino Acid levels in the Rat Brain
    Article Snippet: Within each sample, 100 μl was injected into an ESA CoulArray Model 5600 HPLC system, consisting of two Model 582 pumps, one dynamic gradient mixer, two PEEK pulse dampers, a Model 542 refrigerated autosampler injector, a CoulArray organizer module, and a serial array of 16 coulometric electrodes. .. Mobile Phase B consisted of 0.1 M lithium acetate (Sigma-Aldrich, Inc.) in a solvent mixture of methanol (Avantor Performance Materials, Inc.), acetonitrile (Honeywell, Inc.) and isopropanol (Mallinckrodt Baker, Inc.) at the ratio of 80/10/10 (v/v/v). pH was adjusted to 6.5 using acetic acid (Sigma-Aldrich, Inc.).

    Article Title: New Pre-reacted Glass Containing Dental Composites (giomers) with Improved Fluoride Release and Biocompatibility
    Article Snippet: The analyses were carried out on a Jasco Chromatograph (Jasco International Co., LTD., Tokyo, Japan) that was equipped with an intelligent HPLC pump (PU-980, Jasco International Co., LTD., Tokyo, Japan), a ternary gradient unit (LG-980-02, Jasco International Co., LTD., Tokyo, Japan), an intelligent column thermostat (CO-2060 Plus, Jasco International Co., LTD., Tokyo, Japan), an intelligent UV/VIS detector (UV-975, Jasco International Co., LTD., Tokyo, Japan), and an injection valve that was equipped with a 20 μL sample loop (Rheodyne, Thermo Fischer Scientific, Waltham, MA, USA). .. The mobile phase was a mixture of acetonitrile (A, HPLC grade) and water (B, Milipore ultrapure water), and a gradient was applied according to the following method: 0–15 min. linear gradient 50–80% A; 15–25 min. linear gradient 80–50% A.

    Article Title: SYSTEMIC ADMINISTRATION OF LEPTIN DECREASES PLASMA CORTICOSTERONE LEVELS
    Article Snippet: The mobile phase was made in pyrogen-free water and then filtered and degassed through a Milli-Q purification system (Millipore, Bedford, MA, USA) and pumped at a flow rate of 1.8 ml/min. .. Seventy five μl of the supernatant along with 25 μl of the internal standard (0.5 M dihydroxybenzylamine) was injected into the HPLC system.

    In Vivo:

    Article Title: Tuning Pharmacokinetics to Improve Tumor Accumulation of a Prostate-Specific Membrane Antigen-Targeted Phototheranostic Agent
    Article Snippet: The strip was then placed into a capped test tube containing mobile phase prepared with 2 vol % EDTA (Sigma-Aldrich Co. LLC) and 10 vol % 0.1 M NH4 OAc in ddH2 O. .. In addition, previous reports indicate that copper chelation into a porphyrin ring did not significantly change the in vivo uptake and clearance profiles.

    Radioactivity:

    Article Title: Tuning Pharmacokinetics to Improve Tumor Accumulation of a Prostate-Specific Membrane Antigen-Targeted Phototheranostic Agent
    Article Snippet: Paragraph title: 64 Cu Radiolabeling. ... The strip was then placed into a capped test tube containing mobile phase prepared with 2 vol % EDTA (Sigma-Aldrich Co. LLC) and 10 vol % 0.1 M NH4 OAc in ddH2 O.

    Fluorescence:

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: The analysis was performed using an Eicom 700 system (Eicom Corporation, Kyoto, Japan) consisting of a Shimatzu RF-20AX fluorescence detector (Shimadzu, Kyoto, Japan), an Eicom 700 Insight autosampler, and Envision integration software. .. Chromatographic conditions were set as previously described., Briefly, 10 μL aliquots of each extracted sample were injected into the column and eluted with a mobile phase consisting of 10 mM KH2 PO4 /acetonitrile (3:1 v/v; EMD Millipore, AX0145P1) (pH 4.0 adjusted with 1 M phosphoric acid), at a flow rate of 500 μL/min.

    Mouse Assay:

    Article Title: Response to Fungal Dysbiosis by Gut Resident CX3CR1+ Mononuclear Phagocytes Aggravates Allergic Airway Disease
    Article Snippet: Mouse serum (10 uL) was mixed with mobile phase B (0.1 % formic acid (Sigma-Aldrich) in acetonitrile (Sigma-Aldrich), 100 μL) and internal standard solution (fluconazole-d4 (1.63 μM in acetonitrile) 100 μL, Cayman Chemical). .. The standard curve for fluconazole was created using serum from untreated mice.

    Affinity Column:

    Article Title: Proteoform-Resolved FcɤRIIIa Binding Assay for Fab Glycosylated Monoclonal Antibodies Achieved by Affinity Chromatography Mass Spectrometry of Fc Moieties
    Article Snippet: Preparation of mobile phase was performed with ammonium acetate solution (7.5 M, Sigma-Aldrich, Steinheim Germany) and glacial acetic acid (Honeywell, Seelze, Germany). .. A reference standard therapeutic mAb produced in CHO cells (referred to as mAb1) and the FcɤRIIIa affinity column was obtained from Roche Diagnostics (Penzberg, Germany).

    Purification:

    Article Title: Tuning Pharmacokinetics to Improve Tumor Accumulation of a Prostate-Specific Membrane Antigen-Targeted Phototheranostic Agent
    Article Snippet: The strip was then placed into a capped test tube containing mobile phase prepared with 2 vol % EDTA (Sigma-Aldrich Co. LLC) and 10 vol % 0.1 M NH4 OAc in ddH2 O. .. The developed iTLC strip was cut in thirds and the 64 Cu radioactivity assayed for the two top (free 64 Cu) and separately for a bottom piece (64 Cu-LC-Pyro) using a Wizard 1480 well-type automatic gamma counter (PerkinElmer Inc.; Shelton, CT, USA) and radiochemical purity was further evaluated by radio HPLC performed on a XBridge-C18 column (2.5 μ m, 4.6 mm × 50 mm) with UV detector and radioactivity detector Figure S. Further purification of 64 Cu-LC-Pyro was deemed unnecessary, due to the high molar ratio of LC-Pyro:64 CuCl2 (~1000:1) and its high radiochemical purity.

    Article Title: SYSTEMIC ADMINISTRATION OF LEPTIN DECREASES PLASMA CORTICOSTERONE LEVELS
    Article Snippet: .. The mobile phase was made in pyrogen-free water and then filtered and degassed through a Milli-Q purification system (Millipore, Bedford, MA, USA) and pumped at a flow rate of 1.8 ml/min. ..

    Liquid Chromatography:

    Article Title: Production Standard and Stability of Compounded Del Nido Cardioplegia Solution
    Article Snippet: Paragraph title: Liquid Chromatography ... Both were maintained to 38°C throughout the entire analysis, eluted with a mobile phase of 50% v/v pure milliQ water with 0.1% v/v formic acid CovaChem (Sigma-Aldrich, St Louis, Missouri), and 50% v/v methanol Lichrosolv (Sigma-Aldrich, St Louis, Missouri).

    Software:

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: The analysis was performed using an Eicom 700 system (Eicom Corporation, Kyoto, Japan) consisting of a Shimatzu RF-20AX fluorescence detector (Shimadzu, Kyoto, Japan), an Eicom 700 Insight autosampler, and Envision integration software. .. Chromatographic conditions were set as previously described., Briefly, 10 μL aliquots of each extracted sample were injected into the column and eluted with a mobile phase consisting of 10 mM KH2 PO4 /acetonitrile (3:1 v/v; EMD Millipore, AX0145P1) (pH 4.0 adjusted with 1 M phosphoric acid), at a flow rate of 500 μL/min.

    Article Title: Comparison of Transitional vs Surgical Menopause on Monoamine and Amino Acid levels in the Rat Brain
    Article Snippet: The system was controlled and chromatograms were analyzed using the ESA CoulArray for Windows-32 software program. .. Mobile Phase B consisted of 0.1 M lithium acetate (Sigma-Aldrich, Inc.) in a solvent mixture of methanol (Avantor Performance Materials, Inc.), acetonitrile (Honeywell, Inc.) and isopropanol (Mallinckrodt Baker, Inc.) at the ratio of 80/10/10 (v/v/v). pH was adjusted to 6.5 using acetic acid (Sigma-Aldrich, Inc.).

    Article Title: New Pre-reacted Glass Containing Dental Composites (giomers) with Improved Fluoride Release and Biocompatibility
    Article Snippet: The system was controlled and the experimental data analyzed with the ChromPass software (version v1.7, Jasco International Co., LTD., Tokyo, Japan). .. The mobile phase was a mixture of acetonitrile (A, HPLC grade) and water (B, Milipore ultrapure water), and a gradient was applied according to the following method: 0–15 min. linear gradient 50–80% A; 15–25 min. linear gradient 80–50% A.

    Article Title: SYSTEMIC ADMINISTRATION OF LEPTIN DECREASES PLASMA CORTICOSTERONE LEVELS
    Article Snippet: The mobile phase was made in pyrogen-free water and then filtered and degassed through a Milli-Q purification system (Millipore, Bedford, MA, USA) and pumped at a flow rate of 1.8 ml/min. .. Class VP software (version 7.3, Shimadzu, Columbia, MD) was used to analyze the chromatograms.

    Sample Prep:

    Article Title: Response to Fungal Dysbiosis by Gut Resident CX3CR1+ Mononuclear Phagocytes Aggravates Allergic Airway Disease
    Article Snippet: Paragraph title: LC-MS/MS Sample Preparation and Method ... Mouse serum (10 uL) was mixed with mobile phase B (0.1 % formic acid (Sigma-Aldrich) in acetonitrile (Sigma-Aldrich), 100 μL) and internal standard solution (fluconazole-d4 (1.63 μM in acetonitrile) 100 μL, Cayman Chemical).

    Produced:

    Article Title: Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
    Article Snippet: .. Mobile phases consisted out of acetonitrile (HiPerSolv, VWR) for mobile phase A and ddH2 O produced by the in-house Milli-Q system as mobile phase B, both containing 1% formic acid (v /v ) (33015, Sigma). ..

    Article Title: Proteoform-Resolved FcɤRIIIa Binding Assay for Fab Glycosylated Monoclonal Antibodies Achieved by Affinity Chromatography Mass Spectrometry of Fc Moieties
    Article Snippet: Preparation of mobile phase was performed with ammonium acetate solution (7.5 M, Sigma-Aldrich, Steinheim Germany) and glacial acetic acid (Honeywell, Seelze, Germany). .. A reference standard therapeutic mAb produced in CHO cells (referred to as mAb1) and the FcɤRIIIa affinity column was obtained from Roche Diagnostics (Penzberg, Germany).

    Article Title: Graphene Oxide/Polyethylene Glycol-Stick for Thin Film Microextraction of β-Blockers from Human Oral Fluid by Liquid Chromatography-Tandem Mass Spectrometry
    Article Snippet: .. Deionized water used for the mobile phase was produced by Milli-Q system (Millipore Co, Bedford, MA. .. Samples were analyzed using a triple-quadrupole mass spectrometer, Z-spray Quattro-micro (Waters, Manchester, UK) with an electrospray ionization source (ESI).

    Concentration Assay:

    Article Title: New Pre-reacted Glass Containing Dental Composites (giomers) with Improved Fluoride Release and Biocompatibility
    Article Snippet: The samples were diluted at an appropriate concentration, filtered with 0.45 mm polytetrafluoroethylene (PTFE) filters, and then analyzed by High Performance Liquid Chromatography (HPLC). .. The mobile phase was a mixture of acetonitrile (A, HPLC grade) and water (B, Milipore ultrapure water), and a gradient was applied according to the following method: 0–15 min. linear gradient 50–80% A; 15–25 min. linear gradient 80–50% A.

    Thin Layer Chromatography:

    Article Title: Tuning Pharmacokinetics to Improve Tumor Accumulation of a Prostate-Specific Membrane Antigen-Targeted Phototheranostic Agent
    Article Snippet: 64 Cu(Cl)2 solution (pH = 5.5; ~5.0 mCi; 0.5 mL) was then added and the reaction mixture was heated in a water bath at 60 °C for 30 min. After radiolabeling was completed, sample was diluted with 1 mL of saline and the radiochemical purity was analyzed by instant thin layer chromatography (iTLC). .. The strip was then placed into a capped test tube containing mobile phase prepared with 2 vol % EDTA (Sigma-Aldrich Co. LLC) and 10 vol % 0.1 M NH4 OAc in ddH2 O.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Response to Fungal Dysbiosis by Gut Resident CX3CR1+ Mononuclear Phagocytes Aggravates Allergic Airway Disease
    Article Snippet: Mouse serum (10 uL) was mixed with mobile phase B (0.1 % formic acid (Sigma-Aldrich) in acetonitrile (Sigma-Aldrich), 100 μL) and internal standard solution (fluconazole-d4 (1.63 μM in acetonitrile) 100 μL, Cayman Chemical). .. A 1290 Infinity II HPLC system (Agilent Technologies) coupled with a Triple Quad LC/MS (Agilent 6495) was used for analyte separation and identification by tandem mass spectrometry.

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    Millipore mobile phase a
    Mobile Phase A, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mobile phase a/product/Millipore
    Average 95 stars, based on 16 article reviews
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    mobile phase a - by Bioz Stars, 2020-02
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