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New England Biolabs mobii
Mobii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mobii/product/New England Biolabs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mobii - by Bioz Stars, 2020-01
86/100 stars

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Amplification:

Article Title:
Article Snippet: For the region including the mutation 563C-T (263 bp product) the PCR conditions for amplification were similar except for annealing and extension time which were 45 s. Finally, the PCR products were detected in 3 % agarose gels. .. Finally, MobII (New England Biolabs) was used to detect the 563 C > T mutation associated with the Mediterranean variant.

Article Title:
Article Snippet: A 236-bp PCR fragment including the IL-21 rs2221903 polymorphism was amplified using the primers: 5’-GGTACCTGGACACTGACGCCCA-3’ (upstream) and 5’-AAGGCAGTTTAGTGGCGACAGCC-3’ (downstream), a 222-bp PCR fragment including the IL-21 rs907715 polymorphism was amplified using the primers: 5’-CAATGGCTTGGTGTTTGGTAT-3’ (upstream), and 5’-CCTCTTTTCACTTGGA GCATTC-3’ (downstream), a 229-bp PCR fragment including the IL-21R rs2285452 polymorphism was amplified using the primers: 5’-CTGGGCTGTGATGTGA AGAC-3’ (upstream) and 5’-TGGCAGGTGATAAGGAACAA-3’ (downstream), and a 183-bp PCR fragment including the IL-21R rs3093301 polymorphism was amplified using the primers: 5’-CCCTCCCTCTTTCTTTGTTAG-3’ (upstream) and 5’-TCCTCCTACCTCGGCCTCTCAAAGTG-3’ (downstream). .. The products were digested with restriction enzyme HpyCH4 V, MobII, MluI, BsrGI (New England Biolabs) at 37°C for 2 hours, and analyzed on a 8% polyacrylamide gel electrophoresis.

Mutagenesis:

Article Title:
Article Snippet: .. Finally, MobII (New England Biolabs) was used to detect the 563 C > T mutation associated with the Mediterranean variant. .. The digested products were analysed through electrophoresis on 3 % agarose gels.

Electrophoresis:

Article Title:
Article Snippet: Finally, MobII (New England Biolabs) was used to detect the 563 C > T mutation associated with the Mediterranean variant. .. The digested products were analysed through electrophoresis on 3 % agarose gels.

Polyacrylamide Gel Electrophoresis:

Article Title:
Article Snippet: .. The products were digested with restriction enzyme HpyCH4 V, MobII, MluI, BsrGI (New England Biolabs) at 37°C for 2 hours, and analyzed on a 8% polyacrylamide gel electrophoresis. ..

Polymerase Chain Reaction:

Article Title:
Article Snippet: The PCR products were used in RFLP with specific restriction enzymes. .. Finally, MobII (New England Biolabs) was used to detect the 563 C > T mutation associated with the Mediterranean variant.

Article Title:
Article Snippet: PCR was carried out in a volume of 20 μL containing 3.0 μL DNA, 1.0 μL of each primer, 12.5 μL 2∗Taq PCR MasterMix (TianGen Biotech Co. Ltd, Beijing, China) and 7.5 μL ddH2 O. PCR was carried out with initial denaturation for 3 minutes at 94°C, followed by 30 (IL-21 rs907715 and rs2221903) or 35 (IL-21R rs2285452 and rs3093301) cycles of denaturation for 30 seconds at 94°C, annealing for 30 seconds at 60°C (IL-21 rs2221903), 55°C (IL-21 rs907715), 56°C (IL-21R rs3093301 and rs2285452), extension for 30 seconds at 72°C, and a final extension for 10 minutes at 72°C. .. The products were digested with restriction enzyme HpyCH4 V, MobII, MluI, BsrGI (New England Biolabs) at 37°C for 2 hours, and analyzed on a 8% polyacrylamide gel electrophoresis.

Variant Assay:

Article Title:
Article Snippet: .. Finally, MobII (New England Biolabs) was used to detect the 563 C > T mutation associated with the Mediterranean variant. .. The digested products were analysed through electrophoresis on 3 % agarose gels.

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    New England Biolabs mobii
    Mobii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mobii/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mobii - by Bioz Stars, 2020-01
    86/100 stars
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