mnase i (TaKaRa)

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TaKaRa mnase i
Mnase I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mnase i/product/TaKaRa
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99

mnase i - by Bioz Stars, 2020-08

96/100 stars

Related Products / Commonly Used Together

mononucleosomes
micrococcal nuclease buffer
mnase buffer
mnase i digestion
micrococcal nuclease
chromatin pellets

Images

Lysis:

Article Title: High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells
Article Snippet: .. Briefly, ∼1.5 × 108 cells were resuspended in lysis buffer and digested with micrococcal nuclease (Takara) for approximately 5 min at 37 °C. .. Then, the lysate was immunoprecipitated with the following antibodies: anti-H3K4me2 (Millipore 07-030), anti-H3K4me3 (Abcam ab8580), and anti-H3K27me3 (Millipore 07-449).

Incubation:

Article Title: RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs
Article Snippet: .. When indicated, the supernatant was incubated with 40 units of micrococcal nuclease (Takara) in the presence of 2.5 mM CaCl2 for 30 min at 37°C prior to the incubation with the M2-agarose beads. .. The mixture was filtered by using a mini chromatography column (Bio-Rad).

Article Title: A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway
Article Snippet: .. Fractions with peak RT activity and corresponding fractions from uninfected cells were incubated for 30 min at 37°C with 40 U of micrococcal nuclease (Takara Biotech) in 20 mM Tris-HCl (pH 8.0), 50 mM NaCl, 2.5 mM CaCl2 , and 0.5× PBS plus residual iodixanol. .. Aliquots were digested in the same buffer with 0.1% Triton X-100 (Sigma).

Article Title: Chromatin targeting of nuclear pore proteins induces chromatin decondensation
Article Snippet: .. The nuclei were then resuspended in 200 μl MNase buffer (15 mM Tris, pH 7.4; 60 mM KCl; 15 mM NaCl; 2 mM CaCl2 ; 0.5 mM DTT; and 25% glycerol) and incubated for 10 min at 37°C before the addition of 120 U MNase (#2910A; Takara). .. Digestion was conducted at 37°C for 30 min. For each of the conditions, we ran in parallel an undigested sample with no MNase enzyme that was used for normalization purposes during qPCR analysis.

Activity Assay:

Centrifugation:

Article Title: LATS2 Positively Regulates Polycomb Repressive Complex 2
Article Snippet: .. After centrifugation at 1800 g at 4°C for 10 min, pellets were washed twice with buffer A, resuspended in buffer B (50 mM Tris-HCl [pH 8.0] and 1.5 mM CaCl2 ), and finally treated with 30 units of micrococcal nuclease (Takara Bio, Shiga, Japan) for 35 min at 37°C under mild agitation. .. Solubilized proteins were clarified by two rounds of centrifugation at 5000 g at 4°C for 2 min. Before immunoprecipitation, the chromatin fraction was adjusted to a final concentration of 150 mM sodium chloride and 0.5% Triton X-100.

Article Title: Carboxyl-Terminal Amino Acids 1052 to 1082 of the Latency-Associated Nuclear Antigen (LANA) Interact with RBP-J? and Are Responsible for LANA-Mediated RTA Repression
Article Snippet: .. The extracted nuclei were pelleted by low-speed centrifugation and resuspended in 800 μl of buffer B (10 mM Tris [pH 7.5], 10 mM NaCl, 3 mM MgCl2 , and 1 mM CaCl2 ), and 50 U of micrococcal endonuclease (Takara, Japan) was added. .. After the incubation, 3 mM EGTA (pH 7.8) (final concentration) was added to terminate the reaction, and the solution was spun at 10,000 × g for 5 min at 4°C.

Agarose Gel Electrophoresis:

Article Title: Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots
Article Snippet: .. Genomic DNA was treated with 0, 20, or 30 units of micrococcal nuclease (MNase) (Takara), digested with Sac I, and separated on a 1.2% agarose gel. .. The probe was amplified by PCR (see below), and labeled with 32 P using the Random Primer DNA Labeling Kit version 2 (Takara).