mnase i (TaKaRa)

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Name:

Micrococcal Nuclease

Description:

Micrococcal Nuclease is an endonuclease that preferentially digests single stranded DNA or RNA especially at AT or AU rich regions The enzyme will also digest double stranded DNA or RNA making it an essential component of chromatin immunoprecipitation ChIP assays Micrococcal Nuclease digests 5 phosphodiester bonds of DNA and RNA yielding 3 phosphate mononucleotides and oligonucleotides This enzyme requires Ca2 as a cofactor for its activity and is completely inactivitated by EDTA or EGTA

Catalog Number:

2910a

Price:

None

Size:

15 000 Units

Category:

Micrococcal Nuclease Nucleases Modifying enzymes Cloning

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Structured Review

TaKaRa mnase i
Micrococcal Nuclease is an endonuclease that preferentially digests single stranded DNA or RNA especially at AT or AU rich regions The enzyme will also digest double stranded DNA or RNA making it an essential component of chromatin immunoprecipitation ChIP assays Micrococcal Nuclease digests 5 phosphodiester bonds of DNA and RNA yielding 3 phosphate mononucleotides and oligonucleotides This enzyme requires Ca2 as a cofactor for its activity and is completely inactivitated by EDTA or EGTA
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mnase i - by Bioz Stars, 2021-01

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Related Products / Commonly Used Together

mononucleosomes
micrococcal nuclease buffer
mnase buffer
mnase i digestion
micrococcal nuclease
chromatin pellets

Images

Centrifugation:

Article Title: Carboxyl-Terminal Amino Acids 1052 to 1082 of the Latency-Associated Nuclear Antigen (LANA) Interact with RBP-J? and Are Responsible for LANA-Mediated RTA Repression
Article Snippet: .. The extracted nuclei were pelleted by low-speed centrifugation and resuspended in 800 μl of buffer B (10 mM Tris [pH 7.5], 10 mM NaCl, 3 mM MgCl2 , and 1 mM CaCl2 ), and 50 U of micrococcal endonuclease (Takara, Japan) was added. .. After the incubation, 3 mM EGTA (pH 7.8) (final concentration) was added to terminate the reaction, and the solution was spun at 10,000 × g for 5 min at 4°C.

Agarose Gel Electrophoresis:

Article Title: Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots
Article Snippet: .. Genomic DNA was treated with 0, 20, or 30 units of micrococcal nuclease (MNase) (Takara), digested with Sac I, and separated on a 1.2% agarose gel. .. The probe was amplified by PCR (see below), and labeled with 32 P using the Random Primer DNA Labeling Kit version 2 (Takara).

Isolation:

Article Title: CaWRKY40b in Pepper Acts as a Negative Regulator in Response to Ralstonia solanacearum by Directly Modulating Defense Genes Including CaWRKY40
Article Snippet: .. Nuclear extracts were isolated and were resuspended with the extraction buffer I (0.4 M sucrose, 10 mM Tris-HCl, pH 8.0, 10 mM MgCl2 , 5 mM β-mercaptoethanol, 1 U protease Inhibitors), II (0.25 M sucrose, 10 mM Tris-Cl, pH 8.0, 10 mM MgCl2 , 1% Triton X-100, 5 mM β-mercaptoethanol, 1 µL protease inhibitors), and III (1.7 M sucrose, 10 mM Tris-HCl, pH 8.0, 2 mM MgCl2 , 0.15% Triton X-100, 5 mM β-mercaptoethanol, 1 µL protease Inhibitors) sequentially, and then digested with micrococcal nuclease (Takara, Dalian, China), according to the manufacturer’s instructions. .. Magnetic beads (Invitrogen, Carlsbad, CA, USA) linked to the antibody of HA (anti-HA tag rabbit polyclonal antibody, Sigma, St. Louis, MO, USA) were added to the digested samples, and then eluted.

Purification:

Article Title: Novel bat adenoviruses with an extremely large E3 gene.
Article Snippet: .. Purified viral particles were dissolved in 500 µl lysis buffer (200mM Tris-HCl, 1% TritonX-100, pH 8.0) and incubated at 37 C for 30min with 2000Uml 1 of micrococcal nuclease (Takara) to eliminate cellular DNA and RNA. .. SDS (pH8.0) and proteinase K were added at concentrations of 0.6% and 0.3mg ml 1, respectively, and the reaction mixture was incubated at 37 C for over 3 h. Viral DNA was extracted with phenol/chloroform and precipitated with an equal volume of isopropanol.

Incubation:

Article Title: Chromatin targeting of nuclear pore proteins induces chromatin decondensation
Article Snippet: .. The nuclei were then resuspended in 200 μl MNase buffer (15 mM Tris, pH 7.4; 60 mM KCl; 15 mM NaCl; 2 mM CaCl2 ; 0.5 mM DTT; and 25% glycerol) and incubated for 10 min at 37°C before the addition of 120 U MNase (#2910A; Takara). .. Digestion was conducted at 37°C for 30 min. For each of the conditions, we ran in parallel an undigested sample with no MNase enzyme that was used for normalization purposes during qPCR analysis.

Lysis:

Article Title: High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells
Article Snippet: .. Briefly, ∼1.5 × 108 cells were resuspended in lysis buffer and digested with micrococcal nuclease (Takara) for approximately 5 min at 37 °C. .. Then, the lysate was immunoprecipitated with the following antibodies: anti-H3K4me2 (Millipore 07-030), anti-H3K4me3 (Abcam ab8580), and anti-H3K27me3 (Millipore 07-449).