mnase i (TaKaRa)
99
Name:
Micrococcal Nuclease
Description:
Micrococcal Nuclease is an endonuclease that preferentially digests single stranded DNA or RNA especially at AT or AU rich regions The enzyme will also digest double stranded DNA or RNA making it an essential component of chromatin immunoprecipitation ChIP assays Micrococcal Nuclease digests 5 phosphodiester bonds of DNA and RNA yielding 3 phosphate mononucleotides and oligonucleotides This enzyme requires Ca2 as a cofactor for its activity and is completely inactivitated by EDTA or EGTA
Catalog Number:
2910a
Price:
None
Size:
15 000 Units
Category:
Micrococcal Nuclease Nucleases Modifying enzymes Cloning
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Structured Review
TaKaRa
mnase i
Micrococcal Nuclease is an endonuclease that preferentially digests single stranded DNA or RNA especially at AT or AU rich regions The enzyme will also digest double stranded DNA or RNA making it an essential component of chromatin immunoprecipitation ChIP assays Micrococcal Nuclease digests 5 phosphodiester bonds of DNA and RNA yielding 3 phosphate mononucleotides and oligonucleotides This enzyme requires Ca2 as a cofactor for its activity and is completely inactivitated by EDTA or EGTA
https://www.bioz.com/result/mnase i/product/TaKaRa
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Micrococcal Nuclease is an endonuclease that preferentially digests single stranded DNA or RNA especially at AT or AU rich regions The enzyme will also digest double stranded DNA or RNA making it an essential component of chromatin immunoprecipitation ChIP assays Micrococcal Nuclease digests 5 phosphodiester bonds of DNA and RNA yielding 3 phosphate mononucleotides and oligonucleotides This enzyme requires Ca2 as a cofactor for its activity and is completely inactivitated by EDTA or EGTA
https://www.bioz.com/result/mnase i/product/TaKaRa
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mnase i - by Bioz Stars,
2021-01
99/100 stars
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Centrifugation:Article Title: Carboxyl-Terminal Amino Acids 1052 to 1082 of the Latency-Associated Nuclear Antigen (LANA) Interact with RBP-J? and Are Responsible for LANA-Mediated RTA Repression Article Snippet: .. The extracted nuclei were pelleted by low-speed centrifugation and resuspended in 800 μl of buffer B (10 mM Tris [pH 7.5], 10 mM NaCl, 3 mM MgCl2 , and 1 mM CaCl2 ), and 50 U of Agarose Gel Electrophoresis:Article Title: Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots Article Snippet: .. Genomic DNA was treated with 0, 20, or 30 units of Isolation:Article Title: CaWRKY40b in Pepper Acts as a Negative Regulator in Response to Ralstonia solanacearum by Directly Modulating Defense Genes Including CaWRKY40 Article Snippet: .. Nuclear extracts were isolated and were resuspended with the extraction buffer I (0.4 M sucrose, 10 mM Tris-HCl, pH 8.0, 10 mM MgCl2 , 5 mM β-mercaptoethanol, 1 U protease Inhibitors), II (0.25 M sucrose, 10 mM Tris-Cl, pH 8.0, 10 mM MgCl2 , 1% Triton X-100, 5 mM β-mercaptoethanol, 1 µL protease inhibitors), and III (1.7 M sucrose, 10 mM Tris-HCl, pH 8.0, 2 mM MgCl2 , 0.15% Triton X-100, 5 mM β-mercaptoethanol, 1 µL protease Inhibitors) sequentially, and then digested with Purification:Article Title: Novel bat adenoviruses with an extremely large E3 gene. Article Snippet: .. Purified viral particles were dissolved in 500 µl lysis buffer (200mM Tris-HCl, 1% TritonX-100, pH 8.0) and incubated at 37 C for 30min with 2000Uml 1 of Incubation:Article Title: Chromatin targeting of nuclear pore proteins induces chromatin decondensation Article Snippet: .. The nuclei were then resuspended in 200 μl MNase buffer (15 mM Tris, pH 7.4; 60 mM KCl; 15 mM NaCl; 2 mM CaCl2 ; 0.5 mM DTT; and 25% glycerol) and incubated for 10 min at 37°C before the addition of 120 U Article Title: Novel bat adenoviruses with an extremely large E3 gene. Article Snippet: .. Purified viral particles were dissolved in 500 µl lysis buffer (200mM Tris-HCl, 1% TritonX-100, pH 8.0) and incubated at 37 C for 30min with 2000Uml 1 of Lysis:Article Title: High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells Article Snippet: .. Briefly, ∼1.5 × 108 cells were resuspended in lysis buffer and digested with Article Title: Novel bat adenoviruses with an extremely large E3 gene. Article Snippet: .. Purified viral particles were dissolved in 500 µl lysis buffer (200mM Tris-HCl, 1% TritonX-100, pH 8.0) and incubated at 37 C for 30min with 2000Uml 1 of |