Structured Review

Roche mnase buffer
Mnase Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mnase buffer/product/Roche
Average 90 stars, based on 18 article reviews
Price from $9.99 to $1999.99
mnase buffer - by Bioz Stars, 2020-08
90/100 stars

Images

Related Articles

Centrifugation:

Article Title: Relevance of Simultaneous Mono-Ubiquitinations of Multiple Units of PCNA Homo-Trimers in DNA Damage Tolerance
Article Snippet: .. After removal of detergent-soluble materials by centrifugation, the precipitants were resuspended in microccocal nuclease buffer comprising 20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 0.3 M sucrose, 0.1% Triton X-100, 2 mM MgCl2 , 1 mM CaCl2 , 1× EDTA-free Complete protease inhibitor cocktail (Roche), and 2.5 U of micrococcal nuclease (Roche). .. The solubilized (chromatin) fractions were mixed with Ni-NTA agarose (Qiagen) at 4°C in binding buffer (20 mM sodium phosphate (pH 7.2), 10% glycerol, 0.1% Triton X-100, 0.25 mM phenylmethylsulfonyl fluoride, and 20 mM imidazole) containing 0.5 M NaCl.

Article Title: DNMT3A R882 mutants interact with polycomb proteins to block haematopoietic stem and leukaemic cell differentiation
Article Snippet: .. Cell pellet was resuspended in MNase buffer (10 mM NaCl, 10 mM Tris-HCl (pH 7.5), 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, Roche protease inhibitor cocktail (Roche) and 1 mM PMSF) and kept at 4 °C on ice for 10 min. After centrifugation, the supernatant was discharged and the remaining pellet was resuspended in MNase buffer (10 mM NaCl, 10 mM Tris-HCl (pH 7.5), 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, 1 M CaCl2 , Roche protease inhibitor cocktail (Roche) and 1 mM PMSF), and subjected to MNase digestion (New England Biolabs). .. The digestion was stopped by adding EDTA and diluted with buffer-2 (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5 mM EGTA, 1% Triton X-100, 0.1% sodium-deoxycholate, 0.1% SDS and protease inhibitor cocktail), followed by ultrasonication.

Homogenization:

Article Title: Genome-Wide Target Analyses of Otx2 Homeoprotein in Postnatal Cortex
Article Snippet: .. Pooled cortices from 10 males and 10 females (total 20 mice) were cross-linked with 1% formaldehyde in PBS for 15 min at room temperature and ChIP was performed using the ChIP-IT High Sensitivity kit (Active Motif) per the manufacturer's instructions, with some modifications: after homogenization using a Dounce homogenizer, nuclei were washed in LB0 buffer (20 mM Tris-HCl [pH7.5], 10 mM NaCl, 1 mM EDTA, 0.2% NP-40, 1 mM PMSF) and resuspended in MNase buffer (10 mM Tris-HCl, pH7.5, 10 mM NaCl, 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, 1 mM PMSF), provided with cOmplete™ EDTA-free protease inhibitor cocktail (Roche Diagnostics). ..

Protease Inhibitor:

Article Title: CTCF confers local nucleosome resiliency after DNA replication and during mitosis
Article Snippet: .. Fixed cells were resuspended in 500 µl of MNase buffer (50 mM Tris-HCl pH8, 1 mM CaCl2, 0.2% Triton X-100) supplemented with protease inhibitor cocktail (PIC; 04 693 116 001 Roche). .. Cells were pre-incubated for 10 min at 37°C with 16U of MNase (Expressed in Kunitz, 1 Kunitz is equivalent to 10 gel units, NEB M0247S) added to the reaction.

Article Title: Relevance of Simultaneous Mono-Ubiquitinations of Multiple Units of PCNA Homo-Trimers in DNA Damage Tolerance
Article Snippet: .. After removal of detergent-soluble materials by centrifugation, the precipitants were resuspended in microccocal nuclease buffer comprising 20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 0.3 M sucrose, 0.1% Triton X-100, 2 mM MgCl2 , 1 mM CaCl2 , 1× EDTA-free Complete protease inhibitor cocktail (Roche), and 2.5 U of micrococcal nuclease (Roche). .. The solubilized (chromatin) fractions were mixed with Ni-NTA agarose (Qiagen) at 4°C in binding buffer (20 mM sodium phosphate (pH 7.2), 10% glycerol, 0.1% Triton X-100, 0.25 mM phenylmethylsulfonyl fluoride, and 20 mM imidazole) containing 0.5 M NaCl.

Article Title: Genome-Wide Target Analyses of Otx2 Homeoprotein in Postnatal Cortex
Article Snippet: .. Pooled cortices from 10 males and 10 females (total 20 mice) were cross-linked with 1% formaldehyde in PBS for 15 min at room temperature and ChIP was performed using the ChIP-IT High Sensitivity kit (Active Motif) per the manufacturer's instructions, with some modifications: after homogenization using a Dounce homogenizer, nuclei were washed in LB0 buffer (20 mM Tris-HCl [pH7.5], 10 mM NaCl, 1 mM EDTA, 0.2% NP-40, 1 mM PMSF) and resuspended in MNase buffer (10 mM Tris-HCl, pH7.5, 10 mM NaCl, 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, 1 mM PMSF), provided with cOmplete™ EDTA-free protease inhibitor cocktail (Roche Diagnostics). ..

Article Title: DNMT3A R882 mutants interact with polycomb proteins to block haematopoietic stem and leukaemic cell differentiation
Article Snippet: .. Cell pellet was resuspended in MNase buffer (10 mM NaCl, 10 mM Tris-HCl (pH 7.5), 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, Roche protease inhibitor cocktail (Roche) and 1 mM PMSF) and kept at 4 °C on ice for 10 min. After centrifugation, the supernatant was discharged and the remaining pellet was resuspended in MNase buffer (10 mM NaCl, 10 mM Tris-HCl (pH 7.5), 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, 1 M CaCl2 , Roche protease inhibitor cocktail (Roche) and 1 mM PMSF), and subjected to MNase digestion (New England Biolabs). .. The digestion was stopped by adding EDTA and diluted with buffer-2 (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5 mM EGTA, 1% Triton X-100, 0.1% sodium-deoxycholate, 0.1% SDS and protease inhibitor cocktail), followed by ultrasonication.

Article Title: Analysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance
Article Snippet: .. Chromatin fractionation Cell pellets were collected by scraping in 1× PBS with 1× protease inhibitor (cOmplete, Roche) and split in two equal fractions: (A) For the total proteome cells were resuspended in 1× MNase buffer (0.3 M Sucrose, 50 mM Tris pH 7.5, 30 mM KCl, 7.5 mM NaCl, 4 mM MgCl2 , 1 mM CaCl2 , 0.125% NP-40, 0.25% Na-Deoxycholate) with 1× protease inhibitor and 10 U of MNase (10107921001, Roche) for every 5 million cells. .. Cells were incubated for 30 min at 37 °C, boiled in 1× SDS-loading buffer for 5 min, spun down at 16,000×g for 5 min and the supernatant was collected for western blot. (B) For chromatin-bound and soluble fractions cells were resuspended in chromatin extraction buffer (10 mM Hepes pH 7.6, 3 mM MgCl2 , 0.5% Triton-X-100, 1 mM DTT) with 1× protease inhibitor.

Article Title: Analysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance
Article Snippet: .. Cell pellets were collected by scraping in 1× PBS with 1× protease inhibitor (cOmplete, Roche) and split in two equal fractions: (A) For the total proteome cells were resuspended in 1× MNase buffer (0.3 M Sucrose, 50 mM Tris pH 7.5, 30 mM KCl, 7.5 mM NaCl, 4 mM MgCl2 , 1 mM CaCl2 , 0.125% NP-40, 0.25% Na-Deoxycholate) with 1× protease inhibitor and 10 U of MNase (10107921001, Roche) for every 5 million cells. .. Cells were incubated for 30 min at 37 °C, boiled in 1× SDS-loading buffer for 5 min, spun down at 16,000× g for 5 min and the supernatant was collected for western blot. (B) For chromatin-bound and soluble fractions cells were resuspended in chromatin extraction buffer (10 mM Hepes pH 7.6, 3 mM MgCl2 , 0.5% Triton-X-100, 1 mM DTT) with 1× protease inhibitor.

Mouse Assay:

Article Title: Genome-Wide Target Analyses of Otx2 Homeoprotein in Postnatal Cortex
Article Snippet: .. Pooled cortices from 10 males and 10 females (total 20 mice) were cross-linked with 1% formaldehyde in PBS for 15 min at room temperature and ChIP was performed using the ChIP-IT High Sensitivity kit (Active Motif) per the manufacturer's instructions, with some modifications: after homogenization using a Dounce homogenizer, nuclei were washed in LB0 buffer (20 mM Tris-HCl [pH7.5], 10 mM NaCl, 1 mM EDTA, 0.2% NP-40, 1 mM PMSF) and resuspended in MNase buffer (10 mM Tris-HCl, pH7.5, 10 mM NaCl, 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, 1 mM PMSF), provided with cOmplete™ EDTA-free protease inhibitor cocktail (Roche Diagnostics). ..

Incubation:

Article Title: TGF-? and IL-6 signals modulate chromatin binding and promoter occupancy by acetylated FOXP3
Article Snippet: .. The pellet was dispersed in 100 μl of MNase buffer with or without 10 units of MNase (Roche) and was incubated at room temperature for 30 min with shaking, followed by the addition of 1 mM EGTA. .. After centrifugation at 800 × g , after nuclease digestion, genomic DNA was isolated after RNase A and proteinase K treatment by using a QIAamp DNA mini kit for genomic DNA purification (Qiagen).

Article Title: Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis
Article Snippet: .. MNase digestion was performed on the buffer 1 or 2 lysates by the addition of 150 μl MNase buffer (10 mM Tris, pH 7.5, 10 mM KCl, and 1 mM CaCl2 ) and 15 U of micrococcal nuclease S7 (Roche), and incubation at 37°C for the indicated times. .. Reaction was stopped by 5 mM EDTA (final concentration).

Fractionation:

Article Title: Analysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance
Article Snippet: .. Chromatin fractionation Cell pellets were collected by scraping in 1× PBS with 1× protease inhibitor (cOmplete, Roche) and split in two equal fractions: (A) For the total proteome cells were resuspended in 1× MNase buffer (0.3 M Sucrose, 50 mM Tris pH 7.5, 30 mM KCl, 7.5 mM NaCl, 4 mM MgCl2 , 1 mM CaCl2 , 0.125% NP-40, 0.25% Na-Deoxycholate) with 1× protease inhibitor and 10 U of MNase (10107921001, Roche) for every 5 million cells. .. Cells were incubated for 30 min at 37 °C, boiled in 1× SDS-loading buffer for 5 min, spun down at 16,000×g for 5 min and the supernatant was collected for western blot. (B) For chromatin-bound and soluble fractions cells were resuspended in chromatin extraction buffer (10 mM Hepes pH 7.6, 3 mM MgCl2 , 0.5% Triton-X-100, 1 mM DTT) with 1× protease inhibitor.

Chromatin Immunoprecipitation:

Article Title: Genome-Wide Target Analyses of Otx2 Homeoprotein in Postnatal Cortex
Article Snippet: .. Pooled cortices from 10 males and 10 females (total 20 mice) were cross-linked with 1% formaldehyde in PBS for 15 min at room temperature and ChIP was performed using the ChIP-IT High Sensitivity kit (Active Motif) per the manufacturer's instructions, with some modifications: after homogenization using a Dounce homogenizer, nuclei were washed in LB0 buffer (20 mM Tris-HCl [pH7.5], 10 mM NaCl, 1 mM EDTA, 0.2% NP-40, 1 mM PMSF) and resuspended in MNase buffer (10 mM Tris-HCl, pH7.5, 10 mM NaCl, 2.5 mM MgCl2 , 0.1% NP-40, 1 mM DTT, 1 mM PMSF), provided with cOmplete™ EDTA-free protease inhibitor cocktail (Roche Diagnostics). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Roche mnase buffer
    Mnase Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mnase buffer/product/Roche
    Average 90 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    mnase buffer - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    85
    Roche micrococcal nuclease mnase buffer buffer 20 mm tris hcl
    Micrococcal Nuclease Mnase Buffer Buffer 20 Mm Tris Hcl, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micrococcal nuclease mnase buffer buffer 20 mm tris hcl/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    micrococcal nuclease mnase buffer buffer 20 mm tris hcl - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results