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A: Heat map indicating the fold change increase in levels of circulatory levels of cytokine milieu and <t>MMP9</t> in patients with PKDL (n = 16) with respect to healthy controls (n = 9). The colour bar represents the range of fold change (1-13), with dark blue representing the highest fold change and white being the baseline. B: Representative immunofluorescence profiles of TNF-α expression from dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (20x and 40x magnification), along with bar scatter plots showing the expression of TNF-α in healthy controls (●, n = 6), patients with PKDL (■, n = 16), either polymorphic (▲, n = 8) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. C: Representative immunofluorescence profiles of IFN-γ expression from dermal biopsies of a healthy control, patient with polymorphic and macular PKDL (20x and 40x magnification) along with bar scatter plots indicating the expression of IFN-γ in healthy controls (●, n = 6), patients with PKDL (■, n = 15), either polymorphic (▲, n = 7) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. D: Representative immunohistochemical profiles of MMP9 in dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (10x and 40x magnification). Bar scatter plot demonstrating the status of MMP9 in healthy controls (●, n = 6), patients with PKDL (■, n = 20), either polymorphic (▲, n = 8) or macular (▼, n = 12) PKDL; each horizontal bar represents the median (IQR), along with correlation of MMP9 with lesional TNF-α (n = 14), IFN-γ (n = 12), E-Cadherin (n = 12), fibronectin (n = 12) and laminin (n = 10) fluorescence positivity/field.
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A: Heat map indicating the fold change increase in levels of circulatory levels of cytokine milieu and <t>MMP9</t> in patients with PKDL (n = 16) with respect to healthy controls (n = 9). The colour bar represents the range of fold change (1-13), with dark blue representing the highest fold change and white being the baseline. B: Representative immunofluorescence profiles of TNF-α expression from dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (20x and 40x magnification), along with bar scatter plots showing the expression of TNF-α in healthy controls (●, n = 6), patients with PKDL (■, n = 16), either polymorphic (▲, n = 8) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. C: Representative immunofluorescence profiles of IFN-γ expression from dermal biopsies of a healthy control, patient with polymorphic and macular PKDL (20x and 40x magnification) along with bar scatter plots indicating the expression of IFN-γ in healthy controls (●, n = 6), patients with PKDL (■, n = 15), either polymorphic (▲, n = 7) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. D: Representative immunohistochemical profiles of MMP9 in dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (10x and 40x magnification). Bar scatter plot demonstrating the status of MMP9 in healthy controls (●, n = 6), patients with PKDL (■, n = 20), either polymorphic (▲, n = 8) or macular (▼, n = 12) PKDL; each horizontal bar represents the median (IQR), along with correlation of MMP9 with lesional TNF-α (n = 14), IFN-γ (n = 12), E-Cadherin (n = 12), fibronectin (n = 12) and laminin (n = 10) fluorescence positivity/field.
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A: Heat map indicating the fold change increase in levels of circulatory levels of cytokine milieu and <t>MMP9</t> in patients with PKDL (n = 16) with respect to healthy controls (n = 9). The colour bar represents the range of fold change (1-13), with dark blue representing the highest fold change and white being the baseline. B: Representative immunofluorescence profiles of TNF-α expression from dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (20x and 40x magnification), along with bar scatter plots showing the expression of TNF-α in healthy controls (●, n = 6), patients with PKDL (■, n = 16), either polymorphic (▲, n = 8) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. C: Representative immunofluorescence profiles of IFN-γ expression from dermal biopsies of a healthy control, patient with polymorphic and macular PKDL (20x and 40x magnification) along with bar scatter plots indicating the expression of IFN-γ in healthy controls (●, n = 6), patients with PKDL (■, n = 15), either polymorphic (▲, n = 7) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. D: Representative immunohistochemical profiles of MMP9 in dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (10x and 40x magnification). Bar scatter plot demonstrating the status of MMP9 in healthy controls (●, n = 6), patients with PKDL (■, n = 20), either polymorphic (▲, n = 8) or macular (▼, n = 12) PKDL; each horizontal bar represents the median (IQR), along with correlation of MMP9 with lesional TNF-α (n = 14), IFN-γ (n = 12), E-Cadherin (n = 12), fibronectin (n = 12) and laminin (n = 10) fluorescence positivity/field.
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A: Heat map indicating the fold change increase in levels of circulatory levels of cytokine milieu and <t>MMP9</t> in patients with PKDL (n = 16) with respect to healthy controls (n = 9). The colour bar represents the range of fold change (1-13), with dark blue representing the highest fold change and white being the baseline. B: Representative immunofluorescence profiles of TNF-α expression from dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (20x and 40x magnification), along with bar scatter plots showing the expression of TNF-α in healthy controls (●, n = 6), patients with PKDL (■, n = 16), either polymorphic (▲, n = 8) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. C: Representative immunofluorescence profiles of IFN-γ expression from dermal biopsies of a healthy control, patient with polymorphic and macular PKDL (20x and 40x magnification) along with bar scatter plots indicating the expression of IFN-γ in healthy controls (●, n = 6), patients with PKDL (■, n = 15), either polymorphic (▲, n = 7) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. D: Representative immunohistochemical profiles of MMP9 in dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (10x and 40x magnification). Bar scatter plot demonstrating the status of MMP9 in healthy controls (●, n = 6), patients with PKDL (■, n = 20), either polymorphic (▲, n = 8) or macular (▼, n = 12) PKDL; each horizontal bar represents the median (IQR), along with correlation of MMP9 with lesional TNF-α (n = 14), IFN-γ (n = 12), E-Cadherin (n = 12), fibronectin (n = 12) and laminin (n = 10) fluorescence positivity/field.
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Image Search Results


A: Heat map indicating the fold change increase in levels of circulatory levels of cytokine milieu and MMP9 in patients with PKDL (n = 16) with respect to healthy controls (n = 9). The colour bar represents the range of fold change (1-13), with dark blue representing the highest fold change and white being the baseline. B: Representative immunofluorescence profiles of TNF-α expression from dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (20x and 40x magnification), along with bar scatter plots showing the expression of TNF-α in healthy controls (●, n = 6), patients with PKDL (■, n = 16), either polymorphic (▲, n = 8) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. C: Representative immunofluorescence profiles of IFN-γ expression from dermal biopsies of a healthy control, patient with polymorphic and macular PKDL (20x and 40x magnification) along with bar scatter plots indicating the expression of IFN-γ in healthy controls (●, n = 6), patients with PKDL (■, n = 15), either polymorphic (▲, n = 7) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. D: Representative immunohistochemical profiles of MMP9 in dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (10x and 40x magnification). Bar scatter plot demonstrating the status of MMP9 in healthy controls (●, n = 6), patients with PKDL (■, n = 20), either polymorphic (▲, n = 8) or macular (▼, n = 12) PKDL; each horizontal bar represents the median (IQR), along with correlation of MMP9 with lesional TNF-α (n = 14), IFN-γ (n = 12), E-Cadherin (n = 12), fibronectin (n = 12) and laminin (n = 10) fluorescence positivity/field.

Journal: PLOS Neglected Tropical Diseases

Article Title: Immune-mediated remodelling of tissue architecture in Post-Kala-Azar Dermal Leishmaniasis (PKDL)

doi: 10.1371/journal.pntd.0014798

Figure Lengend Snippet: A: Heat map indicating the fold change increase in levels of circulatory levels of cytokine milieu and MMP9 in patients with PKDL (n = 16) with respect to healthy controls (n = 9). The colour bar represents the range of fold change (1-13), with dark blue representing the highest fold change and white being the baseline. B: Representative immunofluorescence profiles of TNF-α expression from dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (20x and 40x magnification), along with bar scatter plots showing the expression of TNF-α in healthy controls (●, n = 6), patients with PKDL (■, n = 16), either polymorphic (▲, n = 8) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. C: Representative immunofluorescence profiles of IFN-γ expression from dermal biopsies of a healthy control, patient with polymorphic and macular PKDL (20x and 40x magnification) along with bar scatter plots indicating the expression of IFN-γ in healthy controls (●, n = 6), patients with PKDL (■, n = 15), either polymorphic (▲, n = 7) or macular (▼, n = 8) PKDL; each horizontal bar represents the median (IQR) fluorescence positivity/field. D: Representative immunohistochemical profiles of MMP9 in dermal biopsies of a healthy control, patient with polymorphic or macular PKDL (10x and 40x magnification). Bar scatter plot demonstrating the status of MMP9 in healthy controls (●, n = 6), patients with PKDL (■, n = 20), either polymorphic (▲, n = 8) or macular (▼, n = 12) PKDL; each horizontal bar represents the median (IQR), along with correlation of MMP9 with lesional TNF-α (n = 14), IFN-γ (n = 12), E-Cadherin (n = 12), fibronectin (n = 12) and laminin (n = 10) fluorescence positivity/field.

Article Snippet: All reagents were obtained from Sigma Aldrich (St. Louis, MO, USA) except anti-human E-Cadherin (clone NCH-38), CD8 (clone C6/144B), CD68 (clone PG-M1) [Dako, CA, USA], CD20 (clone L26), MMP 9 (clone EP127) [BioSB, Santa Barbara, CA, USA], anti-human ICAM-1, TNF-α, IFN-γ [BD Biosciences, San Jose, CA, USA], Fibronectin (FN1, A12392), Lamininβ1 (LAMB1, A9827), Collagen IV (Col4A3, A16359), Collagen I (Col1A1, A1352) and Goat anti-rabbit secondary antibody IgG-FITC (AS011) [Abclonal, Woburn, USA], antifadent [Southern Biotech, Birmingham, AL, USA], EnVision FLEX Target Retrieval Solution [Dako, CA, USA] and secondary detection system [DAB + chromogen, ScyTek Laboratories, West Logan, UT, USA], MMP9 Human ELISA Kit (RayBiotech, GA, USA), Bio-Plex Pro Human Chemokine Panel 40-Plex and 48-plex (for IFN-γ and TNF-α, BioRad, CA, USA).

Techniques: Immunofluorescence, Expressing, Control, Fluorescence, Immunohistochemical staining