mmessage mmachine t7 kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mmessage mmachine t7 kit
    Mmessage Mmachine T7 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmessage mmachine t7 kit/product/Thermo Fisher
    Average 92 stars, based on 195 article reviews
    Price from $9.99 to $1999.99
    mmessage mmachine t7 kit - by Bioz Stars, 2020-05
    92/100 stars

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    Related Articles

    Electroporation:

    Article Title: An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells
    Article Snippet: .. In vitro transcription and electroporation Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 μl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 40.5 °C for 25 min, 37.5 °C for 50 min, 40.5 °C 25 min and 37.5 °C for 20 min.

    Generated:

    Article Title: Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture
    Article Snippet: .. Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion). .. The N transcripts were generated using a linearized pKT0-N containing IBV N gene and 3′-UTR as templates.

    Article Title: Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
    Article Snippet: .. Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions, and electroporated into Vero cells with one pulse at 450 V and 50 µF with a Bio-Rad Gene Pulser II electroporator. .. The transfected Vero cells were cultured overnight in 1% FBS-containing DMEM and further cultured in DMEM without FBS.

    Article Title: N-Linked glycosylation of the membrane protein ectodomain regulates infectious bronchitis virus-induced ER stress response, apoptosis and pathogenesis
    Article Snippet: .. Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 µl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 40.5 °C for 25 min, 37.5 °C for 50 min, 40.5 °C 25 min and 37.5 °C for 20 min.

    Article Title: Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120
    Article Snippet: .. 2.3 In vitro transcription, transfection and generation of the rescued virus R-H120 Full-length transcript was generated in vitro using mMESSAGE mMACHINE® T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 μl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 37 °C for 120 min. A similar reaction was performed for the N transcripts, which were generated by using a linearized pMD19-T-N-3′ containing the IBV N gene and the 3′-UTR region as templates.

    Article Title: Proteolytic processing of polyproteins 1a and 1ab between non-structural proteins 10 and 11/12 of Coronavirus infectious bronchitis virus is dispensable for viral replication in cultured cells
    Article Snippet: .. In vitro full-length transcripts were generated using the mMessage mMachine T7 kit (Ambion, Austin, TX) and electroporated into Vero cells with one pulse at 450 V, 50 µF with a Bio-Rad Gene Pulser II electroporator. .. The electroporated Vero cells were cultured overnight in 1% FBS-containing MEM and further cultured in MEM without FBS.

    Article Title: An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells
    Article Snippet: .. In vitro transcription and electroporation Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 μl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 40.5 °C for 25 min, 37.5 °C for 50 min, 40.5 °C 25 min and 37.5 °C for 20 min.

    Synthesized:

    Article Title: Recombinant live attenuated avian coronavirus vaccines with deletions in the accessory genes 3ab and/or 5ab protect against infectious bronchitis in chickens
    Article Snippet: .. In short, capped run-off donor transcripts were synthesized from p-IBV-Δ3ab, p-IBV-Δ5ab and p-IBV-Δ3ab5ab using the mMessage mMachine T7 kit (Ambion by Thermo Fisher Scientific) after Mss I-linearization. .. In vitro transcribed RNA was transfected by electroporation into LR7 cells previously infected with mIBV followed by intra-allantoic injection in ten-day-old embryonated SPF chicken eggs using five eggs per dilution (10−1 –10−5 ).

    Transfection:

    Article Title: Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120
    Article Snippet: .. 2.3 In vitro transcription, transfection and generation of the rescued virus R-H120 Full-length transcript was generated in vitro using mMESSAGE mMACHINE® T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 μl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 37 °C for 120 min. A similar reaction was performed for the N transcripts, which were generated by using a linearized pMD19-T-N-3′ containing the IBV N gene and the 3′-UTR region as templates.

    In Vitro:

    Article Title: Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture
    Article Snippet: .. Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion). .. The N transcripts were generated using a linearized pKT0-N containing IBV N gene and 3′-UTR as templates.

    Article Title: Identification and formation mechanism of a novel noncoding RNA produced by avian infectious bronchitis virus
    Article Snippet: .. Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions, and electroporated into Vero cells with one pulse at 450 V and 50 µF with a Bio-Rad Gene Pulser II electroporator. .. The transfected Vero cells were cultured overnight in 1% FBS-containing DMEM and further cultured in DMEM without FBS.

    Article Title: N-Linked glycosylation of the membrane protein ectodomain regulates infectious bronchitis virus-induced ER stress response, apoptosis and pathogenesis
    Article Snippet: .. Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 µl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 40.5 °C for 25 min, 37.5 °C for 50 min, 40.5 °C 25 min and 37.5 °C for 20 min.

    Article Title: Establishment of reverse genetics system for infectious bronchitis virus attenuated vaccine strain H120
    Article Snippet: .. 2.3 In vitro transcription, transfection and generation of the rescued virus R-H120 Full-length transcript was generated in vitro using mMESSAGE mMACHINE® T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 μl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 37 °C for 120 min. A similar reaction was performed for the N transcripts, which were generated by using a linearized pMD19-T-N-3′ containing the IBV N gene and the 3′-UTR region as templates.

    Article Title: Proteolytic processing of polyproteins 1a and 1ab between non-structural proteins 10 and 11/12 of Coronavirus infectious bronchitis virus is dispensable for viral replication in cultured cells
    Article Snippet: .. In vitro full-length transcripts were generated using the mMessage mMachine T7 kit (Ambion, Austin, TX) and electroporated into Vero cells with one pulse at 450 V, 50 µF with a Bio-Rad Gene Pulser II electroporator. .. The electroporated Vero cells were cultured overnight in 1% FBS-containing MEM and further cultured in MEM without FBS.

    Article Title: An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells
    Article Snippet: .. In vitro transcription and electroporation Full-length transcripts were generated in vitro using the mMessage mMachine T7 kit (Ambion, Austin, TX) according to the manufacturer's instructions with certain modifications. .. Briefly, 30 μl of transcription reaction with a 1:1 ratio of GTP to cap analog was sequentially incubated at 40.5 °C for 25 min, 37.5 °C for 50 min, 40.5 °C 25 min and 37.5 °C for 20 min.

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  • 96
    Thermo Fisher mmessage mmachine t7 ultra transcription kit
    Mmessage Mmachine T7 Ultra Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmessage mmachine t7 ultra transcription kit/product/Thermo Fisher
    Average 96 stars, based on 360 article reviews
    Price from $9.99 to $1999.99
    mmessage mmachine t7 ultra transcription kit - by Bioz Stars, 2020-05
    96/100 stars
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