mmei restrictase  (New England Biolabs)


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    Structured Review

    New England Biolabs mmei restrictase
    Mmei Restrictase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmei restrictase/product/New England Biolabs
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mmei restrictase - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Paired-end Tag:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: .. The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags. .. The ditags were PCR amplified, adapters were removed by digestion with NlaIII and separated from ditags in a 12% polyacrylamide gel, and the purified ditags were ligated to form linear concatemers.

    Amplification:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags. .. The ditags were PCR amplified, adapters were removed by digestion with NlaIII and separated from ditags in a 12% polyacrylamide gel, and the purified ditags were ligated to form linear concatemers.

    Clone Assay:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags. .. The obtained concatemers, containing abundant representative cDNA fragments, were ligated into the pZErO-1 vector (Invitrogen) and cloned into E. coli cells.

    Magnetic Beads:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The isolated RNA was incubated with magnetic beads (Dynabeads) and used to synthesize double-stranded cDNA according to a standard protocol. .. The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags.

    Isolation:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The isolated RNA was incubated with magnetic beads (Dynabeads) and used to synthesize double-stranded cDNA according to a standard protocol. .. The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags.

    Purification:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags. .. The ditags were PCR amplified, adapters were removed by digestion with NlaIII and separated from ditags in a 12% polyacrylamide gel, and the purified ditags were ligated to form linear concatemers.

    Incubation:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The isolated RNA was incubated with magnetic beads (Dynabeads) and used to synthesize double-stranded cDNA according to a standard protocol. .. The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags.

    Expressing:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: Paragraph title: 2.5. Serial Analysis of Gene Expression ... The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags.

    Polymerase Chain Reaction:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags. .. The ditags were PCR amplified, adapters were removed by digestion with NlaIII and separated from ditags in a 12% polyacrylamide gel, and the purified ditags were ligated to form linear concatemers.

    Recombinant:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags. .. Independent recombinant clones were used to generate clone libraries arrayed in 96-well plates.

    Plasmid Preparation:

    Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
    Article Snippet: The ligated fragments were then treated with MmeI restrictase (NEB) to produce tags; the two mixtures were pooled together, and the tags were ligated to each other to give ditags. .. The obtained concatemers, containing abundant representative cDNA fragments, were ligated into the pZErO-1 vector (Invitrogen) and cloned into E. coli cells.

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  • 87
    New England Biolabs mmei restrictase
    Mmei Restrictase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmei restrictase/product/New England Biolabs
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mmei restrictase - by Bioz Stars, 2020-04
    87/100 stars
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