mixed cellulose esters membrane discs  (Millipore)


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    Structured Review

    Millipore mixed cellulose esters membrane discs
    Mixed Cellulose Esters Membrane Discs, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mixed cellulose esters membrane discs/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mixed cellulose esters membrane discs - by Bioz Stars, 2020-04
    95/100 stars

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    Electroporation:

    Article Title: MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
    Article Snippet: .. The assembled samples were then drop-dialyzed against 50 ml dH2 O using 0.05 μm mixed cellulose esters membrane discs (Millipore) at room temperature for 1 h. 5 μl of the dialyzed sample was transformed into 25 μl NEB 10-beta electrocompetent cells (NEB) by electroporation at 0.9 kV 100 Ω 25 μF using a Gene Pulser and 1 mm electroporation cuvettes (Bio-Rad). .. Following electroporation, 1 ml LB medium was added to the cells and incubated at 37°C 220 rpm for 1 h. Cells were plated on LB plates with GIX selection (gentamicin 2.5 μg/ml, IPTG 100 μM, X-gal 50 μg/ml) at 37°C overnight.

    Selection:

    Article Title: MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
    Article Snippet: Assembly reactions were transformed into normal DH10B chemical competent cells, and plated on LB plates with AIX selection (ampicillin 100 μg/ml, IPTG 100 μM, X-gal 50 μg/ml) at 37°C overnight. .. The assembled samples were then drop-dialyzed against 50 ml dH2 O using 0.05 μm mixed cellulose esters membrane discs (Millipore) at room temperature for 1 h. 5 μl of the dialyzed sample was transformed into 25 μl NEB 10-beta electrocompetent cells (NEB) by electroporation at 0.9 kV 100 Ω 25 μF using a Gene Pulser and 1 mm electroporation cuvettes (Bio-Rad).

    Incubation:

    Article Title: MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
    Article Snippet: The assembled samples were then drop-dialyzed against 50 ml dH2 O using 0.05 μm mixed cellulose esters membrane discs (Millipore) at room temperature for 1 h. 5 μl of the dialyzed sample was transformed into 25 μl NEB 10-beta electrocompetent cells (NEB) by electroporation at 0.9 kV 100 Ω 25 μF using a Gene Pulser and 1 mm electroporation cuvettes (Bio-Rad). .. Following electroporation, 1 ml LB medium was added to the cells and incubated at 37°C 220 rpm for 1 h. Cells were plated on LB plates with GIX selection (gentamicin 2.5 μg/ml, IPTG 100 μM, X-gal 50 μg/ml) at 37°C overnight.

    DNA Sequencing:

    Article Title: MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
    Article Snippet: White colonies were expanded and screened by restriction digestion using the corresponding restriction enzyme and by DNA sequencing. .. The assembled samples were then drop-dialyzed against 50 ml dH2 O using 0.05 μm mixed cellulose esters membrane discs (Millipore) at room temperature for 1 h. 5 μl of the dialyzed sample was transformed into 25 μl NEB 10-beta electrocompetent cells (NEB) by electroporation at 0.9 kV 100 Ω 25 μF using a Gene Pulser and 1 mm electroporation cuvettes (Bio-Rad).

    Transformation Assay:

    Article Title: MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
    Article Snippet: .. The assembled samples were then drop-dialyzed against 50 ml dH2 O using 0.05 μm mixed cellulose esters membrane discs (Millipore) at room temperature for 1 h. 5 μl of the dialyzed sample was transformed into 25 μl NEB 10-beta electrocompetent cells (NEB) by electroporation at 0.9 kV 100 Ω 25 μF using a Gene Pulser and 1 mm electroporation cuvettes (Bio-Rad). .. Following electroporation, 1 ml LB medium was added to the cells and incubated at 37°C 220 rpm for 1 h. Cells were plated on LB plates with GIX selection (gentamicin 2.5 μg/ml, IPTG 100 μM, X-gal 50 μg/ml) at 37°C overnight.

    Plasmid Preparation:

    Article Title: MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
    Article Snippet: In stage one, the fragments were assembled, seven per group, using 15 fmol of each donor plasmid prepared from DH10B cells, 15 fmol assembly vector prepared from DH10B-M.Osp807II cells, 1000 U T4 ligase (NEB), and 5 U BsaI (NEB) in 20 μl 1× T4 ligase buffer (NEB). .. The assembled samples were then drop-dialyzed against 50 ml dH2 O using 0.05 μm mixed cellulose esters membrane discs (Millipore) at room temperature for 1 h. 5 μl of the dialyzed sample was transformed into 25 μl NEB 10-beta electrocompetent cells (NEB) by electroporation at 0.9 kV 100 Ω 25 μF using a Gene Pulser and 1 mm electroporation cuvettes (Bio-Rad).

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    Millipore diameter millipore mixed cellulose ester membrane disc
    Diameter Millipore Mixed Cellulose Ester Membrane Disc, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diameter millipore mixed cellulose ester membrane disc/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diameter millipore mixed cellulose ester membrane disc - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    86
    Millipore cellulose ester membrane discs
    Cellulose Ester Membrane Discs, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cellulose ester membrane discs/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cellulose ester membrane discs - by Bioz Stars, 2020-04
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    95
    Millipore mixed cellulose esters membrane discs
    Mixed Cellulose Esters Membrane Discs, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mixed cellulose esters membrane discs/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mixed cellulose esters membrane discs - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    85
    Millipore 13 mm diameter mixed cellulose ester membrane disc
    13 Mm Diameter Mixed Cellulose Ester Membrane Disc, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/13 mm diameter mixed cellulose ester membrane disc/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    13 mm diameter mixed cellulose ester membrane disc - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

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