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mir‑x mirna firststrand synthesis kit  (TaKaRa)


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    TaKaRa mir‑x mirna firststrand synthesis kit
    Mir‑X Mirna Firststrand Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mir‑x mirna firststrand synthesis kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    mir‑x mirna firststrand synthesis kit - by Bioz Stars, 2025-02
    99/100 stars

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    TaKaRa mir‑x mirna firststrand synthesis kit
    Mir‑X Mirna Firststrand Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mir‑x mirna firststrand synthesis kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
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    TaKaRa mir x mirna
    <t>miRNA</t> and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001
    Mir X Mirna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa mir ‑ x mirna
    <t>miRNA</t> and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001
    Mir ‑ X Mirna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa mir x mirna firststrand synthesis kit
    <t>miRNA</t> and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001
    Mir X Mirna Firststrand Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mir x mirna firststrand synthesis kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
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    TaKaRa mir x mirna qrt pcr tb green kit
    <t>miRNA</t> and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001
    Mir X Mirna Qrt Pcr Tb Green Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa first‑strand synthesis kit
    <t>miRNA</t> and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001
    First‑Strand Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa mir‑x mirna qrt‑pcr tb green kit
    <t>miRNA</t> and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001
    Mir‑X Mirna Qrt‑Pcr Tb Green Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mir‑x mirna qrt‑pcr tb green kit/product/TaKaRa
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    TaKaRa first strand synthesis kit
    <t>miRNA</t> and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001
    First Strand Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/first strand synthesis kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    first strand synthesis kit - by Bioz Stars, 2025-02
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    Image Search Results


    miRNA and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001

    Journal: Molecular Medicine

    Article Title: Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury

    doi: 10.1186/s10020-025-01111-x

    Figure Lengend Snippet: miRNA and protein analysis of plasma-EVs. (A) TF-miRNA-mRNA regulatory networks were constructed for sepsis based on their inside interaction relationships. (B) The GO enrichment analysis of the target genes of the eight upregulated miRNAs. (C) The KEGG enrichment analysis of the target genes of the eight upregulated miRNAs. (D) Observation of EV morphology by TEM; Scale bar: 100 nm. (E) NanoFCM of EV diameter. (F) Western blot analysis of the expression of EV markers (Alix, Tsg101, and CD9) and Non-EV markers (GM130, calnexin, and TIM23) in cells and EVs. (G) Validation of the expression levels of miR-30e-3p, miR-99a-5p, miR-122-5p, miR-125b-5p, miR-150-5p, miR-155-5p, miR-223-3p, and miR-378a-3p in plasma-EVs by RT-qPCR. (H) Validation of the expression levels of PMN-related proteins (CD177, SLPI, OLFM4 and LCN2) in plasma-EVs by ELISA. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001

    Article Snippet: The cells were also subjected to total RNA isolation using RNAiso Plus (TaKaRa, Dalian, China).The Mir-X™ miRNA First-Strand Synthesis Kit (TaKaRa, Dalian, China) and Reverse Transcription Kit (TaKaRa, Dalian, China) were utilized for reverse transcription of miRNAs and mRNAs respectively.

    Techniques: Construct, Western Blot, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Validation of miRNA and PMN-related protein expression in plasma-EVs from survivors and non-survivors. (A-B) Sepsis patients were categorized into survivors ( n = 60) and non-survivors ( n = 55) based on 28-day prognosis. Validation of the expression levels of miR-99a-5p, miR-122-5p, miR-125b-5p, miR-223-3p, CD177, SLPI, OLFM4 and LCN2 in plasma-EVs. (C-D) A total of 64 septic ARDS were stratified into survivors ( n = 27) and non-survivors ( n = 37). Subsets analysis of the levels of miR-99a-5p, miR-122-5p, miR-125b-5p, miR-223-3p, CD177, SLPI, OLFM4 and LCN2 in 28-day prognosis. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001

    Journal: Molecular Medicine

    Article Title: Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury

    doi: 10.1186/s10020-025-01111-x

    Figure Lengend Snippet: Validation of miRNA and PMN-related protein expression in plasma-EVs from survivors and non-survivors. (A-B) Sepsis patients were categorized into survivors ( n = 60) and non-survivors ( n = 55) based on 28-day prognosis. Validation of the expression levels of miR-99a-5p, miR-122-5p, miR-125b-5p, miR-223-3p, CD177, SLPI, OLFM4 and LCN2 in plasma-EVs. (C-D) A total of 64 septic ARDS were stratified into survivors ( n = 27) and non-survivors ( n = 37). Subsets analysis of the levels of miR-99a-5p, miR-122-5p, miR-125b-5p, miR-223-3p, CD177, SLPI, OLFM4 and LCN2 in 28-day prognosis. Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001

    Article Snippet: The cells were also subjected to total RNA isolation using RNAiso Plus (TaKaRa, Dalian, China).The Mir-X™ miRNA First-Strand Synthesis Kit (TaKaRa, Dalian, China) and Reverse Transcription Kit (TaKaRa, Dalian, China) were utilized for reverse transcription of miRNAs and mRNAs respectively.

    Techniques: Expressing

    ROC curves and Kaplan-Meier survival estimation of miRNAs and PMN-related proteins from plasma-EVs for septic patients. ( A - D ) ROC curves were drawn based on RT-qPCR and ELISA data for septic shock, septic ARDS, and 28-day mortality in septic patients or septic ARDS. AUC indicates area under curve. ( E - J ) Kaplan-Meier survival curves according to different levels of miRNAs and PMN-related proteins from plasma-EVs in sepsis. The miRNAs or proteins were dichotomized at the median based on a low-versus-high expression (E, miR-122-5p; F, miR-125b-5p; G, miR-223-3p; H, LCN2; I, OLFM4; J, miR-122-5p in septic ARDS) for 28-day mortality

    Journal: Molecular Medicine

    Article Title: Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury

    doi: 10.1186/s10020-025-01111-x

    Figure Lengend Snippet: ROC curves and Kaplan-Meier survival estimation of miRNAs and PMN-related proteins from plasma-EVs for septic patients. ( A - D ) ROC curves were drawn based on RT-qPCR and ELISA data for septic shock, septic ARDS, and 28-day mortality in septic patients or septic ARDS. AUC indicates area under curve. ( E - J ) Kaplan-Meier survival curves according to different levels of miRNAs and PMN-related proteins from plasma-EVs in sepsis. The miRNAs or proteins were dichotomized at the median based on a low-versus-high expression (E, miR-122-5p; F, miR-125b-5p; G, miR-223-3p; H, LCN2; I, OLFM4; J, miR-122-5p in septic ARDS) for 28-day mortality

    Article Snippet: The cells were also subjected to total RNA isolation using RNAiso Plus (TaKaRa, Dalian, China).The Mir-X™ miRNA First-Strand Synthesis Kit (TaKaRa, Dalian, China) and Reverse Transcription Kit (TaKaRa, Dalian, China) were utilized for reverse transcription of miRNAs and mRNAs respectively.

    Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing

    miR-223-3p activates AMs by targeting MEF2C. (A) RT-qPCR detection of the expression of the four selected miRNAs in plasma-EVs. (B) RT-qPCR detection of the expression of the four miRNAs in AMs co-cultured with PBS-EVs or LPS-EVs for 24 h. (C) Uptake of PKH67-labeled EVs (green) by AMs co-incubated for 6 h, observed under inverted fuorescence microscopy. (D) The intersection of predicted target genes of miR-223-3p from three databases (StarBase, TarBase, miRDB). (E) KEGG pathway analysis was performed on potential target genes of miR-223-3p. (F) Conservation of the miR-223-3p target sequence in MEF2C 3ʹUTR among different species and conservation of the miR-223-3p sequence among different species. (G) Dual-luciferase reporter assay performed in HEK293T cells. Cells were co-transfected with dual-luciferase reporter plasmids containing the wild-type or mutant MEF2C 3ʹUTR sequence, along with the NC or miR-223-3p mimic. (H) MEF2C expression was determined by RT-qPCR in cells transfected with miR-223-3p mimic or NC mimic 24 h post-transfection. (I) MEF2C expression was determined by RT-qPCR in AMs treated with PBS-EVs or LPS-EVs, respectively. Representative results from three independent experiments are shown ( n = 6 except for n = 3 in G, H,I). Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001

    Journal: Molecular Medicine

    Article Title: Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury

    doi: 10.1186/s10020-025-01111-x

    Figure Lengend Snippet: miR-223-3p activates AMs by targeting MEF2C. (A) RT-qPCR detection of the expression of the four selected miRNAs in plasma-EVs. (B) RT-qPCR detection of the expression of the four miRNAs in AMs co-cultured with PBS-EVs or LPS-EVs for 24 h. (C) Uptake of PKH67-labeled EVs (green) by AMs co-incubated for 6 h, observed under inverted fuorescence microscopy. (D) The intersection of predicted target genes of miR-223-3p from three databases (StarBase, TarBase, miRDB). (E) KEGG pathway analysis was performed on potential target genes of miR-223-3p. (F) Conservation of the miR-223-3p target sequence in MEF2C 3ʹUTR among different species and conservation of the miR-223-3p sequence among different species. (G) Dual-luciferase reporter assay performed in HEK293T cells. Cells were co-transfected with dual-luciferase reporter plasmids containing the wild-type or mutant MEF2C 3ʹUTR sequence, along with the NC or miR-223-3p mimic. (H) MEF2C expression was determined by RT-qPCR in cells transfected with miR-223-3p mimic or NC mimic 24 h post-transfection. (I) MEF2C expression was determined by RT-qPCR in AMs treated with PBS-EVs or LPS-EVs, respectively. Representative results from three independent experiments are shown ( n = 6 except for n = 3 in G, H,I). Data are presented as the mean ± SEM. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.001,**** p < 0.0001

    Article Snippet: The cells were also subjected to total RNA isolation using RNAiso Plus (TaKaRa, Dalian, China).The Mir-X™ miRNA First-Strand Synthesis Kit (TaKaRa, Dalian, China) and Reverse Transcription Kit (TaKaRa, Dalian, China) were utilized for reverse transcription of miRNAs and mRNAs respectively.

    Techniques: Quantitative RT-PCR, Expressing, Cell Culture, Labeling, Incubation, Microscopy, Sequencing, Luciferase, Reporter Assay, Transfection, Mutagenesis

    Flow chart. The differential expression analysis of miRNAs and PMN-related proteins indicated that the plasma-EV model has potential for predicting septic ARDS and prognosis among septic patients. Schematic representation of the mechanism by which plasma-derived EVs induce AM autophagy and ferroptosis in septic ALI. Created with Figdraw.com

    Journal: Molecular Medicine

    Article Title: Plasma-derived extracellular vesicles prime alveolar macrophages for autophagy and ferroptosis in sepsis-induced acute lung injury

    doi: 10.1186/s10020-025-01111-x

    Figure Lengend Snippet: Flow chart. The differential expression analysis of miRNAs and PMN-related proteins indicated that the plasma-EV model has potential for predicting septic ARDS and prognosis among septic patients. Schematic representation of the mechanism by which plasma-derived EVs induce AM autophagy and ferroptosis in septic ALI. Created with Figdraw.com

    Article Snippet: The cells were also subjected to total RNA isolation using RNAiso Plus (TaKaRa, Dalian, China).The Mir-X™ miRNA First-Strand Synthesis Kit (TaKaRa, Dalian, China) and Reverse Transcription Kit (TaKaRa, Dalian, China) were utilized for reverse transcription of miRNAs and mRNAs respectively.

    Techniques: Expressing, Derivative Assay