Structured Review

Roche miniquick spin rna columns
Miniquick Spin Rna Columns, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miniquick spin rna columns/product/Roche
Average 91 stars, based on 6 article reviews
Price from $9.99 to $1999.99
miniquick spin rna columns - by Bioz Stars, 2020-05
91/100 stars

Images

Related Articles

Ethanol Precipitation:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: .. The synthesized RNA was then column purified using the miniQuick Spin RNA columns, followed by phenol/chloroform extraction, ethanol precipitation, and lastly, dissolution in DNase/Rnase-free water. gRNA (50 ng/μL) was co-injected with 500 ng/μL Cas9 mRNA into D . magna eggs as mentioned above. .. The eye phenotypes of G1 offspring produced by injected G0 mothers were observed under a stereomicroscope.

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

Synthesized:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: .. The synthesized RNA was then column purified using the miniQuick Spin RNA columns, followed by phenol/chloroform extraction, ethanol precipitation, and lastly, dissolution in DNase/Rnase-free water. gRNA (50 ng/μL) was co-injected with 500 ng/μL Cas9 mRNA into D . magna eggs as mentioned above. .. The eye phenotypes of G1 offspring produced by injected G0 mothers were observed under a stereomicroscope.

In Vitro:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

Purification:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: .. The synthesized RNA was then column purified using the miniQuick Spin RNA columns, followed by phenol/chloroform extraction, ethanol precipitation, and lastly, dissolution in DNase/Rnase-free water. gRNA (50 ng/μL) was co-injected with 500 ng/μL Cas9 mRNA into D . magna eggs as mentioned above. .. The eye phenotypes of G1 offspring produced by injected G0 mothers were observed under a stereomicroscope.

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

Article Title: Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter
Article Snippet: .. Further, the products underwent a three-step purification using miniQuick Spin RNA columns (Roche Diagnostics GmbH, Mannheim, Germany), phenol/chloroform purification, and ethanol purification, before being dissolved in DNase/RNase-free water (Life Technologies). .. Construction of donor vector The donor vector consisted of three main elements: i) a recognition sequence for the designed TALEN pair (48 bp), ii) a full coding sequence of the red fluorescent protein-encoding gene mCherry (711 bp), and iii) a full-length sequence of dsx1 3′ UTR cloned directly from the last exon of the host animal (2,458 bp).

Article Title: A role for fast rhythmic bursting neurons in cortical gamma oscillations in vitro
Article Snippet: .. Riboprobes were purified by using miniquick-spin RNA columns (Roche Diagnostics, Mannheim, Germany) and stored at -70°C. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Roche miniquick spin rna columns
    Miniquick Spin Rna Columns, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miniquick spin rna columns/product/Roche
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    miniquick spin rna columns - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    Image Search Results