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Roche miniquick spin rna columns
Miniquick Spin Rna Columns, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miniquick spin rna columns/product/Roche
Average 91 stars, based on 6 article reviews
Price from $9.99 to $1999.99
miniquick spin rna columns - by Bioz Stars, 2019-10
91/100 stars

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Clone Assay:

Article Title: A role for fast rhythmic bursting neurons in cortical gamma oscillations in vitro
Article Snippet: DNA templates for px2 spanned the entire ORF ( ) and were cloned into pBluescript SK-. .. Riboprobes were purified by using miniquick-spin RNA columns (Roche Diagnostics, Mannheim, Germany) and stored at -70°C.

Amplification:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

In Vitro:

Article Title: Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter
Article Snippet: Paragraph title: In vitro transcription ... Further, the products underwent a three-step purification using miniQuick Spin RNA columns (Roche Diagnostics GmbH, Mannheim, Germany), phenol/chloroform purification, and ethanol purification, before being dissolved in DNase/RNase-free water (Life Technologies).

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: To synthesize gRNAs in vitro , gRNA synthesis vectors were digested by Dra I (NEW ENGLAND Biolabs, Connecticut, USA) and purified by phenol/chloroform extraction. .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

Article Title: A role for fast rhythmic bursting neurons in cortical gamma oscillations in vitro
Article Snippet: For riboprobe synthesis, the recombinant plasmids were linearized and in vitro transcription was performed with T3(sense) and T7(antisense) polymerase. .. Riboprobes were purified by using miniquick-spin RNA columns (Roche Diagnostics, Mannheim, Germany) and stored at -70°C.

Synthesized:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA).

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Poly(A) tails were attached to the capped Cas9 RNAs using the Poly(A) Tailing Kit (Life Technologies, California, USA). .. The synthesized RNA was then column purified using the miniQuick Spin RNA columns, followed by phenol/chloroform extraction, ethanol precipitation, and lastly, dissolution in DNase/Rnase-free water. gRNA (50 ng/μL) was co-injected with 500 ng/μL Cas9 mRNA into D . magna eggs as mentioned above. .. The eye phenotypes of G1 offspring produced by injected G0 mothers were observed under a stereomicroscope.

Ethanol Precipitation:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: To synthesize gRNAs in vitro , gRNA synthesis vectors were digested by Dra I (NEW ENGLAND Biolabs, Connecticut, USA) and purified by phenol/chloroform extraction. .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Poly(A) tails were attached to the capped Cas9 RNAs using the Poly(A) Tailing Kit (Life Technologies, California, USA). .. The synthesized RNA was then column purified using the miniQuick Spin RNA columns, followed by phenol/chloroform extraction, ethanol precipitation, and lastly, dissolution in DNase/Rnase-free water. gRNA (50 ng/μL) was co-injected with 500 ng/μL Cas9 mRNA into D . magna eggs as mentioned above. .. The eye phenotypes of G1 offspring produced by injected G0 mothers were observed under a stereomicroscope.

Ligation:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: A pair of scarlet- targeting oligonucleotides were then annealed and ligated into the linearized pDR274 vector using a ligation mix (TaKaRa Bio, Shiga, Japan). .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA).

Knock-Out:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Paragraph title: Knockout of Scarlet by CRISPR/Cas9 system ... Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA).

Purification:

Article Title: Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter
Article Snippet: With these templates, capped-transcription was performed using the mMessage mMachine SP6 kit (Life Technologies, California, USA), followed by a tailing reaction using a Poly(A) Tailing kit (Life Technologies). .. Further, the products underwent a three-step purification using miniQuick Spin RNA columns (Roche Diagnostics GmbH, Mannheim, Germany), phenol/chloroform purification, and ethanol purification, before being dissolved in DNase/RNase-free water (Life Technologies). .. The donor vector consisted of three main elements: i) a recognition sequence for the designed TALEN pair (48 bp), ii) a full coding sequence of the red fluorescent protein-encoding gene mCherry (711 bp), and iii) a full-length sequence of dsx1 3′ UTR cloned directly from the last exon of the host animal (2,458 bp).

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: To synthesize gRNAs in vitro , gRNA synthesis vectors were digested by Dra I (NEW ENGLAND Biolabs, Connecticut, USA) and purified by phenol/chloroform extraction. .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

Article Title: A role for fast rhythmic bursting neurons in cortical gamma oscillations in vitro
Article Snippet: For riboprobe synthesis, the recombinant plasmids were linearized and in vitro transcription was performed with T3(sense) and T7(antisense) polymerase. .. Riboprobes were purified by using miniquick-spin RNA columns (Roche Diagnostics, Mannheim, Germany) and stored at -70°C. .. In situ hybridization was performed as described in ref .

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Poly(A) tails were attached to the capped Cas9 RNAs using the Poly(A) Tailing Kit (Life Technologies, California, USA). .. The synthesized RNA was then column purified using the miniQuick Spin RNA columns, followed by phenol/chloroform extraction, ethanol precipitation, and lastly, dissolution in DNase/Rnase-free water. gRNA (50 ng/μL) was co-injected with 500 ng/μL Cas9 mRNA into D . magna eggs as mentioned above. .. The eye phenotypes of G1 offspring produced by injected G0 mothers were observed under a stereomicroscope.

Expressing:

Article Title: Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter
Article Snippet: To generate linear templates for in vitro transcription, the two TALEN expression vectors were first digested with Acc65I, and then subjected to purification using a QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany). .. Further, the products underwent a three-step purification using miniQuick Spin RNA columns (Roche Diagnostics GmbH, Mannheim, Germany), phenol/chloroform purification, and ethanol purification, before being dissolved in DNase/RNase-free water (Life Technologies).

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: To generate the gRNA expression vectors, the plasmid pDR274 (Addgene plasmid 42250, [ ] was digested with Bsa I (NEW ENGLAND Biolabs, Connecticut, USA), followed by dephosphorylation with Antartic Phosphatase (NEW ENGLAND Biolabs, Connecticut, USA). .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA).

CRISPR:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Paragraph title: Knockout of Scarlet by CRISPR/Cas9 system ... Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA).

Polymerase Chain Reaction:

Article Title: Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter
Article Snippet: To generate linear templates for in vitro transcription, the two TALEN expression vectors were first digested with Acc65I, and then subjected to purification using a QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany). .. Further, the products underwent a three-step purification using miniQuick Spin RNA columns (Roche Diagnostics GmbH, Mannheim, Germany), phenol/chloroform purification, and ethanol purification, before being dissolved in DNase/RNase-free water (Life Technologies).

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA). .. For synthesis of Cas9 mRNA, a template with the T7 promoter was amplified by PCR from pCS-Dmavas-Cas9 [ ].

Recombinant:

Article Title: A role for fast rhythmic bursting neurons in cortical gamma oscillations in vitro
Article Snippet: For riboprobe synthesis, the recombinant plasmids were linearized and in vitro transcription was performed with T3(sense) and T7(antisense) polymerase. .. Riboprobes were purified by using miniquick-spin RNA columns (Roche Diagnostics, Mannheim, Germany) and stored at -70°C.

De-Phosphorylation Assay:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: To generate the gRNA expression vectors, the plasmid pDR274 (Addgene plasmid 42250, [ ] was digested with Bsa I (NEW ENGLAND Biolabs, Connecticut, USA), followed by dephosphorylation with Antartic Phosphatase (NEW ENGLAND Biolabs, Connecticut, USA). .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA).

In Situ Hybridization:

Article Title: A role for fast rhythmic bursting neurons in cortical gamma oscillations in vitro
Article Snippet: In situ hybridization was performed by using sense (control) and antisense RNAs for Px2. .. Riboprobes were purified by using miniquick-spin RNA columns (Roche Diagnostics, Mannheim, Germany) and stored at -70°C.

Plasmid Preparation:

Article Title: Generation of white-eyed Daphnia magna mutants lacking scarlet function
Article Snippet: A pair of scarlet- targeting oligonucleotides were then annealed and ligated into the linearized pDR274 vector using a ligation mix (TaKaRa Bio, Shiga, Japan). .. Dra I-digested DNA fragments were used as templates for in vitro transcription with mMessage mMachine T7 Kit (Life Technologies, California, USA), followed by column purification with miniQuick Spin RNA columns (Roche diagnostics GmbH, Mannheim, Germany), phenol/chloroform extraction, ethanol precipitation, and dissolution in DNase/RNase-free water (Life Technologies, California, USA).

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    Roche miniquick spin rna columns
    Miniquick Spin Rna Columns, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miniquick spin rna columns/product/Roche
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    miniquick spin rna columns - by Bioz Stars, 2019-10
    91/100 stars
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