Structured Review

Stratagene minimal e1b promoter luciferase gene
Minimal E1b Promoter Luciferase Gene, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/minimal e1b promoter luciferase gene/product/Stratagene
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
minimal e1b promoter luciferase gene - by Bioz Stars, 2020-09
88/100 stars

Images

Related Articles

Transfection:

Article Title: Triptolide attenuates lipopolysaccharide-induced inflammatory responses in human endothelial cells: involvement of NF-κB pathway
Article Snippet: .. Dual-luciferase reporter assay for NF-κB To determine NF-κB transcriptional activity, HUVECs were transfected with a cis-reporter plasmid containing 5 copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene (pNF-κB Luc; Stratagene, LaJolla, CA, USA) using LipofectAMINE 2000, according to the manufacturer’s protocol. pRL-SV40 Renilla luciferase control reporter vector (Promega Corporation, Madison, WI, USA) was co-transfected as an internal control. .. A total of 24 h after transfection, the cells were treated with LPS in the presence or absence of triptolide at the indicated doses.

Luciferase:

Article Title: Triptolide attenuates lipopolysaccharide-induced inflammatory responses in human endothelial cells: involvement of NF-κB pathway
Article Snippet: .. Dual-luciferase reporter assay for NF-κB To determine NF-κB transcriptional activity, HUVECs were transfected with a cis-reporter plasmid containing 5 copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene (pNF-κB Luc; Stratagene, LaJolla, CA, USA) using LipofectAMINE 2000, according to the manufacturer’s protocol. pRL-SV40 Renilla luciferase control reporter vector (Promega Corporation, Madison, WI, USA) was co-transfected as an internal control. .. A total of 24 h after transfection, the cells were treated with LPS in the presence or absence of triptolide at the indicated doses.

Article Title: Selective Inactivation of Intracellular BiP/GRP78 Attenuates Endothelial Inflammation and Permeability in Acute Lung Injury
Article Snippet: .. Reporter gene constructs and luciferase assay The construct pNF-κB-LUC containing five copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was purchased from Stratagene (La Jolla, CA). .. Reporter gene transfections and luciferase assays were performed essentially as described .

Reporter Assay:

Article Title: Triptolide attenuates lipopolysaccharide-induced inflammatory responses in human endothelial cells: involvement of NF-κB pathway
Article Snippet: .. Dual-luciferase reporter assay for NF-κB To determine NF-κB transcriptional activity, HUVECs were transfected with a cis-reporter plasmid containing 5 copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene (pNF-κB Luc; Stratagene, LaJolla, CA, USA) using LipofectAMINE 2000, according to the manufacturer’s protocol. pRL-SV40 Renilla luciferase control reporter vector (Promega Corporation, Madison, WI, USA) was co-transfected as an internal control. .. A total of 24 h after transfection, the cells were treated with LPS in the presence or absence of triptolide at the indicated doses.

Construct:

Article Title: Preconditioning with Endoplasmic Reticulum Stress Ameliorates Endothelial Cell Inflammation
Article Snippet: .. NF-κB Transcriptional Activity The construct pNF-κB-LUC containing five copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was purchased from Stratagene (La Jolla, CA). .. Transfections were performed using the DEAE-dextran method essentially as described .

Article Title: Importins α and β Signaling Mediates Endothelial Cell Inflammation and Barrier Disruption
Article Snippet: .. The construct pNF-κB-LUC containing five copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was purchased from Stratagene (La Jolla, CA). .. Transfections were performed using the DEAE-dextran method essentially as described [ ].

Article Title: Activation of Syk by Protein Kinase C-? Regulates Thrombin-induced Intercellular Adhesion Molecule-1 Expression in Endothelial Cells via Tyrosine Phosphorylation of RelA/p65 *Activation of Syk by Protein Kinase C-? Regulates Thrombin-induced Intercellular Adhesion Molecule-1 Expression in Endothelial Cells via Tyrosine Phosphorylation of RelA/p65 * S⃞
Article Snippet: .. All other materials were from VWR Scientific Products Corp. (Gaithersburg, MD). cDNA and siRNA Constructs —The construct pNF-κB-LUC containing five copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was obtained from Stratagene (La Jolla, CA). .. The GeneSuppressor plasmid encoding control or Syk siRNA was obtained from Imgenex (San Diego, CA).

Article Title: Selective Inactivation of Intracellular BiP/GRP78 Attenuates Endothelial Inflammation and Permeability in Acute Lung Injury
Article Snippet: .. Reporter gene constructs and luciferase assay The construct pNF-κB-LUC containing five copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was purchased from Stratagene (La Jolla, CA). .. Reporter gene transfections and luciferase assays were performed essentially as described .

Activity Assay:

Article Title: Triptolide attenuates lipopolysaccharide-induced inflammatory responses in human endothelial cells: involvement of NF-κB pathway
Article Snippet: .. Dual-luciferase reporter assay for NF-κB To determine NF-κB transcriptional activity, HUVECs were transfected with a cis-reporter plasmid containing 5 copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene (pNF-κB Luc; Stratagene, LaJolla, CA, USA) using LipofectAMINE 2000, according to the manufacturer’s protocol. pRL-SV40 Renilla luciferase control reporter vector (Promega Corporation, Madison, WI, USA) was co-transfected as an internal control. .. A total of 24 h after transfection, the cells were treated with LPS in the presence or absence of triptolide at the indicated doses.

Article Title: Preconditioning with Endoplasmic Reticulum Stress Ameliorates Endothelial Cell Inflammation
Article Snippet: .. NF-κB Transcriptional Activity The construct pNF-κB-LUC containing five copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was purchased from Stratagene (La Jolla, CA). .. Transfections were performed using the DEAE-dextran method essentially as described .

Plasmid Preparation:

Article Title: Triptolide attenuates lipopolysaccharide-induced inflammatory responses in human endothelial cells: involvement of NF-κB pathway
Article Snippet: .. Dual-luciferase reporter assay for NF-κB To determine NF-κB transcriptional activity, HUVECs were transfected with a cis-reporter plasmid containing 5 copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene (pNF-κB Luc; Stratagene, LaJolla, CA, USA) using LipofectAMINE 2000, according to the manufacturer’s protocol. pRL-SV40 Renilla luciferase control reporter vector (Promega Corporation, Madison, WI, USA) was co-transfected as an internal control. .. A total of 24 h after transfection, the cells were treated with LPS in the presence or absence of triptolide at the indicated doses.

Article Title: Protein Kinase C-? Regulates Thrombin-Induced ICAM-1 Gene Expression in Endothelial Cells via Activation of p38 Mitogen-Activated Protein Kinase
Article Snippet: .. The plasmid pNF-κB-LUC containing five copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was purchased from Stratagene. .. The expression vector pcDNA3 containing tagged kinase-defective PKC-α, -ɛ, and -δ isoforms was a gift from I.

Article Title: Regulation of Endothelial Cell Inflammation and Lung PMN Infiltration by Transglutaminase 2
Article Snippet: .. The plasmid pNF-κBLUC containing 5 copies of consensus NF-κB sequences linked to a minimal E1B promoter-luciferase gene was purchased from Stratagene (La Jolla, CA). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Stratagene minimal e1b promoter luciferase gene
    Minimal E1b Promoter Luciferase Gene, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minimal e1b promoter luciferase gene/product/Stratagene
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    minimal e1b promoter luciferase gene - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results