minikit  (Qiagen)


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    Structured Review

    Qiagen minikit
    Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minikit/product/Qiagen
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    minikit - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a
    Article Snippet: For quantitative RT-PCR (qRT-PCR), RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with the RT2 First Strand kit (both from QIAGEN). .. Reactions were run in 25 μL with SYBR Green qPCR Master Mix (Bio-Rad) as recommended by the manufacturer.

    Article Title: The Expansion of Thymopoiesis in Neonatal Mice Is Dependent on Expression of High Mobility Group A 2 Protein (Hmga2)
    Article Snippet: qPCR RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with RT2 First Strand kit (both from QIAGEN) or TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems). .. PCR reactions were run using SYBR green qPCR master mix (Bio-Rad, Hercules, CA) or TaqMan Universal PCR master mix (Applied Biosystems) as per manufacturer instructions.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a
    Article Snippet: For quantitative RT-PCR (qRT-PCR), RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with the RT2 First Strand kit (both from QIAGEN). .. The presence of single RT-PCR products was confirmed by melt curve analysis.

    RNA Sequencing Assay:

    Article Title: The role of nitric oxide during embryonic epidermis development of Xenopus laevis
    Article Snippet: Paragraph title: RNA-Seq ... Total RNA was isolated using Minikit with standard protocol including DNase treatment (Qiagen, 74104).

    Synthesized:

    Article Title: Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with the RT2 First Strand kit (both from QIAGEN). .. Reactions were run in 25 μL with SYBR Green qPCR Master Mix (Bio-Rad) as recommended by the manufacturer.

    Article Title: The Expansion of Thymopoiesis in Neonatal Mice Is Dependent on Expression of High Mobility Group A 2 Protein (Hmga2)
    Article Snippet: .. qPCR RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with RT2 First Strand kit (both from QIAGEN) or TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems). .. PCR reactions were run using SYBR green qPCR master mix (Bio-Rad, Hercules, CA) or TaqMan Universal PCR master mix (Applied Biosystems) as per manufacturer instructions.

    Isolation:

    Article Title: Role of the Enterococcus faecalis GelE Protease in Determination of Cellular Chain Length, Supernatant Pheromone Levels, and Degradation of Fibrin and Misfolded Surface Proteins
    Article Snippet: .. Plasmids were isolated from E. coli with the Qiagen midi- or minikit as recommended by the manufacturer. .. Plasmids were isolated from E. faecalis , L. lactis , S. pyogenes , and S. gordonii by a slight modification of the Qiagen protocol as previously described ( ).

    Article Title: The role of nitric oxide during embryonic epidermis development of Xenopus laevis
    Article Snippet: .. Total RNA was isolated using Minikit with standard protocol including DNase treatment (Qiagen, 74104). .. Quality of total RNA was tested using Experion High sense chip with standard protocol (Biorad, 7007105).

    Quantitative RT-PCR:

    Article Title: Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a
    Article Snippet: .. For quantitative RT-PCR (qRT-PCR), RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with the RT2 First Strand kit (both from QIAGEN). .. Reactions were run in 25 μL with SYBR Green qPCR Master Mix (Bio-Rad) as recommended by the manufacturer.

    Article Title: The Protozoan Trichomonas vaginalis Targets Bacteria with Laterally Acquired NlpC/P60 Peptidoglycan Hydrolases
    Article Snippet: Paragraph title: Reverse transcription and quantitative PCR (RT-qPCR). ... Total RNA was treated with DNase I (Ambion) and cleaned with a minikit and RNeasy MinElute (Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a
    Article Snippet: For quantitative RT-PCR (qRT-PCR), RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with the RT2 First Strand kit (both from QIAGEN). .. Reactions were run in 25 μL with SYBR Green qPCR Master Mix (Bio-Rad) as recommended by the manufacturer.

    Article Title: The Expansion of Thymopoiesis in Neonatal Mice Is Dependent on Expression of High Mobility Group A 2 Protein (Hmga2)
    Article Snippet: .. qPCR RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with RT2 First Strand kit (both from QIAGEN) or TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems). .. PCR reactions were run using SYBR green qPCR master mix (Bio-Rad, Hercules, CA) or TaqMan Universal PCR master mix (Applied Biosystems) as per manufacturer instructions.

    Article Title: The Protozoan Trichomonas vaginalis Targets Bacteria with Laterally Acquired NlpC/P60 Peptidoglycan Hydrolases
    Article Snippet: Paragraph title: Reverse transcription and quantitative PCR (RT-qPCR). ... Total RNA was treated with DNase I (Ambion) and cleaned with a minikit and RNeasy MinElute (Qiagen).

    Polymerase Chain Reaction:

    Article Title: The Expansion of Thymopoiesis in Neonatal Mice Is Dependent on Expression of High Mobility Group A 2 Protein (Hmga2)
    Article Snippet: qPCR RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with RT2 First Strand kit (both from QIAGEN) or TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems). .. PCR reactions were run using SYBR green qPCR master mix (Bio-Rad, Hercules, CA) or TaqMan Universal PCR master mix (Applied Biosystems) as per manufacturer instructions.

    Article Title: Role of the Enterococcus faecalis GelE Protease in Determination of Cellular Chain Length, Supernatant Pheromone Levels, and Degradation of Fibrin and Misfolded Surface Proteins
    Article Snippet: Plasmids were isolated from E. coli with the Qiagen midi- or minikit as recommended by the manufacturer. .. PCR was performed with a Perkin-Elmer Gene Amp PCR system or an Eppendorf Mastercycler with BioXact DNA polymerase (Bioline).

    Modification:

    Article Title: Role of the Enterococcus faecalis GelE Protease in Determination of Cellular Chain Length, Supernatant Pheromone Levels, and Degradation of Fibrin and Misfolded Surface Proteins
    Article Snippet: Plasmids were isolated from E. coli with the Qiagen midi- or minikit as recommended by the manufacturer. .. Plasmids were isolated from E. faecalis , L. lactis , S. pyogenes , and S. gordonii by a slight modification of the Qiagen protocol as previously described ( ).

    Injection:

    Article Title: The role of nitric oxide during embryonic epidermis development of Xenopus laevis
    Article Snippet: RNA-Seq Embryos were injected at the one-cell stage using 17 ng of nNOS :eNOS MO (1:1) mixture and control embryos with identical amount of control MO. .. Total RNA was isolated using Minikit with standard protocol including DNase treatment (Qiagen, 74104).

    Chromatin Immunoprecipitation:

    Article Title: The role of nitric oxide during embryonic epidermis development of Xenopus laevis
    Article Snippet: Total RNA was isolated using Minikit with standard protocol including DNase treatment (Qiagen, 74104). .. Quality of total RNA was tested using Experion High sense chip with standard protocol (Biorad, 7007105).

    Software:

    Article Title: Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a
    Article Snippet: For quantitative RT-PCR (qRT-PCR), RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with the RT2 First Strand kit (both from QIAGEN). .. Data were analyzed with Bio-Rad IQ5 2.0 software using the Pfaffl method with β- actin or gapdh as a reference gene.

    Article Title: The Expansion of Thymopoiesis in Neonatal Mice Is Dependent on Expression of High Mobility Group A 2 Protein (Hmga2)
    Article Snippet: qPCR RNA was extracted with the RNeasy Plus micro- or minikit and cDNA was synthesized with RT2 First Strand kit (both from QIAGEN) or TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems). .. Data were analyzed with Bio-Rad IQ5 software using the Pfaffl method.

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    Qiagen qiaamp dna stool minikit plus bead beating
    Effect of protocol modifications. (A) Pig feces was extracted using standard as well as modified protocols based on the <t>QIAamp</t> <t>DNA</t> stool <t>minikit</t> and QIAamp Fast DNA stool minikit. The modifications included bead beating, pretreatment of the sample, and transfer of the double amount of volume after cell lysis. In the bead beating step, different bead types were examined (for details, see Materials and Methods; Table 1 ). The alpha diversity (Chao 1 and Shannon index) was determined at OTU level, and the microbial community composition was examined at family level based on 16S rRNA gene profiling. (B) Selected standard and modified DNA extraction protocols were employed to extract DNA from human feces, pig feces, and sewage, and their DNA concentration was displayed in a star plot. The values indicate the averages from duplicate extractions.
    Qiaamp Dna Stool Minikit Plus Bead Beating, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp dna stool minikit plus bead beating/product/Qiagen
    Average 99 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna stool minikit plus bead beating - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Qiagen qiaamp viral rna minikit
    Bland-Altman analysis of HIV-1 viral loads in patient samples ( n = 47) of plasma versus DPS after <t>RNA</t> extraction with a <t>QIAamp</t> viral RNA mini kit (Qiagen) (A), the Abbott sample preparation system (B), and a Nuclisens manual extraction kit (bioMérieux)
    Qiaamp Viral Rna Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp viral rna minikit/product/Qiagen
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    qiaamp viral rna minikit - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Qiagen qiagen rnaeasy minikit
    Expression of TLR mRNA expression in ML-1 cells. Six million cells were incubated in the presence TPA of (5 nM) or vehicle (DMSO) for indicated times. Total RNAs were extracted from cell samples using Trizol reagents, purified with the <t>Qiagen</t> <t>RNAeasy</t> mini kit and analyzed by microarray analysis using human Genome U133 2.0 PlusArray Chip. Six replicates including three controls (DMSO-treated) and three TPA-treated ML-1 cells samples were examined. The levels of (a) TLR1, TLR2, TLR4 and TLR8 and (b) IRAK-2 mRNAs were determined by examination of the microarray analysis data.
    Qiagen Rnaeasy Minikit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rnaeasy minikit/product/Qiagen
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    qiagen rnaeasy minikit - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of protocol modifications. (A) Pig feces was extracted using standard as well as modified protocols based on the QIAamp DNA stool minikit and QIAamp Fast DNA stool minikit. The modifications included bead beating, pretreatment of the sample, and transfer of the double amount of volume after cell lysis. In the bead beating step, different bead types were examined (for details, see Materials and Methods; Table 1 ). The alpha diversity (Chao 1 and Shannon index) was determined at OTU level, and the microbial community composition was examined at family level based on 16S rRNA gene profiling. (B) Selected standard and modified DNA extraction protocols were employed to extract DNA from human feces, pig feces, and sewage, and their DNA concentration was displayed in a star plot. The values indicate the averages from duplicate extractions.

    Journal: mSystems

    Article Title: Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

    doi: 10.1128/mSystems.00095-16

    Figure Lengend Snippet: Effect of protocol modifications. (A) Pig feces was extracted using standard as well as modified protocols based on the QIAamp DNA stool minikit and QIAamp Fast DNA stool minikit. The modifications included bead beating, pretreatment of the sample, and transfer of the double amount of volume after cell lysis. In the bead beating step, different bead types were examined (for details, see Materials and Methods; Table 1 ). The alpha diversity (Chao 1 and Shannon index) was determined at OTU level, and the microbial community composition was examined at family level based on 16S rRNA gene profiling. (B) Selected standard and modified DNA extraction protocols were employed to extract DNA from human feces, pig feces, and sewage, and their DNA concentration was displayed in a star plot. The values indicate the averages from duplicate extractions.

    Article Snippet: In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) ( and details below).

    Techniques: Modification, Lysis, DNA Extraction, Concentration Assay

    Comparison of DNA extraction methods. (A) Experimental design. Human feces, pig feces, and hospital sewage were extracted using seven different DNA extraction methods ( Table 1 ): InnuPure C16, MagNA Pure LC DNA isolation kit III, Easy-DNA gDNA purification kit, MP FastDNA Spin kit, PowerSoil DNA isolation kit, QIAamp DNA stool minikit, and QIAamp DNA stool minikit plus bead beating (for details, see Materials and Methods). DNA concentration, purity, and stability were examined, and microbial community composition was determined using 16S rRNA gene profiling and metagenomics (selected samples). (B) DNA from each method was dissolved in 100 µl solution, and DNA concentrations were determined using Qubit dsDNA BR assay kit measurements. Values represent averages from duplicate or triplicate DNA extractions (see also Table S1A in the supplemental material). (C) Ecological richness (Chao 1) and diversity (Shannon index) were determined based on contingency tables from 16S rRNA gene profiling and metagenomic sequencing data at OTU and species levels, respectively (see also Table S1B ).

    Journal: mSystems

    Article Title: Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome Composition

    doi: 10.1128/mSystems.00095-16

    Figure Lengend Snippet: Comparison of DNA extraction methods. (A) Experimental design. Human feces, pig feces, and hospital sewage were extracted using seven different DNA extraction methods ( Table 1 ): InnuPure C16, MagNA Pure LC DNA isolation kit III, Easy-DNA gDNA purification kit, MP FastDNA Spin kit, PowerSoil DNA isolation kit, QIAamp DNA stool minikit, and QIAamp DNA stool minikit plus bead beating (for details, see Materials and Methods). DNA concentration, purity, and stability were examined, and microbial community composition was determined using 16S rRNA gene profiling and metagenomics (selected samples). (B) DNA from each method was dissolved in 100 µl solution, and DNA concentrations were determined using Qubit dsDNA BR assay kit measurements. Values represent averages from duplicate or triplicate DNA extractions (see also Table S1A in the supplemental material). (C) Ecological richness (Chao 1) and diversity (Shannon index) were determined based on contingency tables from 16S rRNA gene profiling and metagenomic sequencing data at OTU and species levels, respectively (see also Table S1B ).

    Article Snippet: In a first step, seven DNA isolation procedures were examined, namely, InnuPure C16 from Analytic Jena AG (InnuPure), MagNA Pure LC DNA isolation kit III from Roche (MagNA Pure), Easy-DNA genomic DNA (gDNA) purification kit from Invitrogen (Easy-DNA), MP FastDNA Spin kit from MP Biomedicals (FastDNA), PowerSoil DNA isolation kit from MoBio (PowerSoil.HMP), QIAamp DNA stool minikit from Qiagen (QIAStool), and QIAamp DNA stool minikit plus bead beating from Qiagen (QIAStool+BB) ( and details below).

    Techniques: DNA Extraction, Purification, Concentration Assay, Sequencing

    DGGE on DNA extracted by three different treatment methods from faecal samples. Example of DGGE runs of PCR products from five stool samples by different DNA extractions. DGGE profiles (30-65% denaturant) from faecal samples on DNA extracted by three different treatment methods and treatment times. ( B ) pre-treatment by Mini BeadBeater 8 ( T ) pre-treatment by TissueLyser, ( Q ) no pretreatment. All subsequent DNA extractions were performed by the QIAamp DNA stool MiniKit. The agitation time was 4 minutes for the Mini BeadBeater 8, and 6 minutes and 8 minutes for the TissueLyser. (The picture is compiled from two gel images, aligned according to the reference lane comprising an internal standard (not shown)).

    Journal: The Open Microbiology Journal

    Article Title: Optimising Bacterial DNA Extraction from Faecal Samples: Comparison of Three Methods

    doi: 10.2174/1874285801105010014

    Figure Lengend Snippet: DGGE on DNA extracted by three different treatment methods from faecal samples. Example of DGGE runs of PCR products from five stool samples by different DNA extractions. DGGE profiles (30-65% denaturant) from faecal samples on DNA extracted by three different treatment methods and treatment times. ( B ) pre-treatment by Mini BeadBeater 8 ( T ) pre-treatment by TissueLyser, ( Q ) no pretreatment. All subsequent DNA extractions were performed by the QIAamp DNA stool MiniKit. The agitation time was 4 minutes for the Mini BeadBeater 8, and 6 minutes and 8 minutes for the TissueLyser. (The picture is compiled from two gel images, aligned according to the reference lane comprising an internal standard (not shown)).

    Article Snippet: Both procedures were followed by DNA extraction using QIAamp DNA stool MiniKit, (QIAGEN, Hilden, Germany).

    Techniques: Denaturing Gradient Gel Electrophoresis, Polymerase Chain Reaction

    Bland-Altman analysis of HIV-1 viral loads in patient samples ( n = 47) of plasma versus DPS after RNA extraction with a QIAamp viral RNA mini kit (Qiagen) (A), the Abbott sample preparation system (B), and a Nuclisens manual extraction kit (bioMérieux)

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing ▿

    doi: 10.1128/JCM.02255-08

    Figure Lengend Snippet: Bland-Altman analysis of HIV-1 viral loads in patient samples ( n = 47) of plasma versus DPS after RNA extraction with a QIAamp viral RNA mini kit (Qiagen) (A), the Abbott sample preparation system (B), and a Nuclisens manual extraction kit (bioMérieux)

    Article Snippet: The QIAamp viral RNA minikit (Qiagen, Courtaboeuf, France) combines a silica gel-based membrane with the speed of microspin centrifugation.

    Techniques: RNA Extraction, Sample Prep

    HIV-1 viral loads in patient samples ( n = 47) of plasma and DPS after RNA extractions with a QIAamp viral RNA mini kit (Qiagen), the Abbott sample preparation system, or a Nuclisens manual extraction kit (bioMérieux). (A) Comparison of

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing ▿

    doi: 10.1128/JCM.02255-08

    Figure Lengend Snippet: HIV-1 viral loads in patient samples ( n = 47) of plasma and DPS after RNA extractions with a QIAamp viral RNA mini kit (Qiagen), the Abbott sample preparation system, or a Nuclisens manual extraction kit (bioMérieux). (A) Comparison of

    Article Snippet: The QIAamp viral RNA minikit (Qiagen, Courtaboeuf, France) combines a silica gel-based membrane with the speed of microspin centrifugation.

    Techniques: Sample Prep

    Expression of TLR mRNA expression in ML-1 cells. Six million cells were incubated in the presence TPA of (5 nM) or vehicle (DMSO) for indicated times. Total RNAs were extracted from cell samples using Trizol reagents, purified with the Qiagen RNAeasy mini kit and analyzed by microarray analysis using human Genome U133 2.0 PlusArray Chip. Six replicates including three controls (DMSO-treated) and three TPA-treated ML-1 cells samples were examined. The levels of (a) TLR1, TLR2, TLR4 and TLR8 and (b) IRAK-2 mRNAs were determined by examination of the microarray analysis data.

    Journal: ISRN Oncology

    Article Title: Upregulation of TLR1, TLR2, TLR4, and IRAK-2 Expression During ML-1 Cell Differentiation to Macrophages: Role in the Potentiation of Cellular Responses to LPS and LTA

    doi: 10.5402/2012/641246

    Figure Lengend Snippet: Expression of TLR mRNA expression in ML-1 cells. Six million cells were incubated in the presence TPA of (5 nM) or vehicle (DMSO) for indicated times. Total RNAs were extracted from cell samples using Trizol reagents, purified with the Qiagen RNAeasy mini kit and analyzed by microarray analysis using human Genome U133 2.0 PlusArray Chip. Six replicates including three controls (DMSO-treated) and three TPA-treated ML-1 cells samples were examined. The levels of (a) TLR1, TLR2, TLR4 and TLR8 and (b) IRAK-2 mRNAs were determined by examination of the microarray analysis data.

    Article Snippet: Total RNA was extracted using Trizol reagent and purified with the Qiagen RNAeasy minikit.

    Techniques: Expressing, Incubation, Purification, Microarray, Chromatin Immunoprecipitation