minicultures  (Millipore)


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    Millipore minicultures
    Stimulation of alkaloid biosynthesis of emerged-grown cultures by mycelial extract, fraction I2-6, or uric acid. Conidial suspensions in 300 μl of culture liquid were grown in microplate compartments. At the times indicated they received the 3-kDa ultrafiltrate, fraction I2-6, 1 μM uric acid, or fraction I2-6 after uricase treatment, as marked. Mycelial extract and fraction I2-6 were diluted to 1 μM uric acid. The mycelial extract added represented low-MW material extracted from 9.3 mg (dry weight); the final uric acid content was 1 μM. Fraction I2-6 was also present at a final concentration of 1 μM uric acid. Uricase treatment was done under the conditions used for the uric acid assay (see Materials and Methods). Additional columns of the time-zero experiment (termed 1 h) represent <t>minicultures</t> grown from conidiospores that were treated for 1 h with either mycelial extract or 1 μM uric acid, filtered, and resuspended in fresh culture liquid prior to incubation in the microtiter plates. Total alkaloid content was assayed after 8 days of culture as described in Materials and Methods. All data are means with SD, n = 8 wells.
    Minicultures, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minicultures/product/Millipore
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    minicultures - by Bioz Stars, 2020-08
    85/100 stars

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    1) Product Images from "Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus †"

    Article Title: Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus †

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.68.4.1524-1533.2002

    Stimulation of alkaloid biosynthesis of emerged-grown cultures by mycelial extract, fraction I2-6, or uric acid. Conidial suspensions in 300 μl of culture liquid were grown in microplate compartments. At the times indicated they received the 3-kDa ultrafiltrate, fraction I2-6, 1 μM uric acid, or fraction I2-6 after uricase treatment, as marked. Mycelial extract and fraction I2-6 were diluted to 1 μM uric acid. The mycelial extract added represented low-MW material extracted from 9.3 mg (dry weight); the final uric acid content was 1 μM. Fraction I2-6 was also present at a final concentration of 1 μM uric acid. Uricase treatment was done under the conditions used for the uric acid assay (see Materials and Methods). Additional columns of the time-zero experiment (termed 1 h) represent minicultures grown from conidiospores that were treated for 1 h with either mycelial extract or 1 μM uric acid, filtered, and resuspended in fresh culture liquid prior to incubation in the microtiter plates. Total alkaloid content was assayed after 8 days of culture as described in Materials and Methods. All data are means with SD, n = 8 wells.
    Figure Legend Snippet: Stimulation of alkaloid biosynthesis of emerged-grown cultures by mycelial extract, fraction I2-6, or uric acid. Conidial suspensions in 300 μl of culture liquid were grown in microplate compartments. At the times indicated they received the 3-kDa ultrafiltrate, fraction I2-6, 1 μM uric acid, or fraction I2-6 after uricase treatment, as marked. Mycelial extract and fraction I2-6 were diluted to 1 μM uric acid. The mycelial extract added represented low-MW material extracted from 9.3 mg (dry weight); the final uric acid content was 1 μM. Fraction I2-6 was also present at a final concentration of 1 μM uric acid. Uricase treatment was done under the conditions used for the uric acid assay (see Materials and Methods). Additional columns of the time-zero experiment (termed 1 h) represent minicultures grown from conidiospores that were treated for 1 h with either mycelial extract or 1 μM uric acid, filtered, and resuspended in fresh culture liquid prior to incubation in the microtiter plates. Total alkaloid content was assayed after 8 days of culture as described in Materials and Methods. All data are means with SD, n = 8 wells.

    Techniques Used: Concentration Assay, Uric Acid Assay, Incubation

    Related Articles

    Isolation:

    Article Title: Methylation of the Corticotropin Releasing Hormone Gene Promoter in BeWo Cells: Relationship to Gene Activity
    Article Snippet: .. Plasmids were isolated from the minicultures using the GenElute plasmid Miniprep Kit (Sigma-Aldrich, Castle Hill, NSW, Australia). .. Plasmid DNA purity and yield were assessed by UV absorption.

    Plasmid Preparation:

    Article Title: Methylation of the Corticotropin Releasing Hormone Gene Promoter in BeWo Cells: Relationship to Gene Activity
    Article Snippet: .. Plasmids were isolated from the minicultures using the GenElute plasmid Miniprep Kit (Sigma-Aldrich, Castle Hill, NSW, Australia). .. Plasmid DNA purity and yield were assessed by UV absorption.

    BAC Assay:

    Article Title: Error-Prone ZW Pairing and No Evidence for Meiotic Sex Chromosome Inactivation in the Chicken Germ Line
    Article Snippet: .. BAC DNA was extracted from minicultures using the PhasePrep BAC DNA kit (Sigma) and about 2 µg of DNA was labelled with digoxigenin, using a DIG-Nick translation kit (Roche). .. The DNA probes were purified and used for the RNA FISH experiments as described in Mahadevaiah et al., 2009 .

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    Millipore minicultures
    Stimulation of alkaloid biosynthesis of emerged-grown cultures by mycelial extract, fraction I2-6, or uric acid. Conidial suspensions in 300 μl of culture liquid were grown in microplate compartments. At the times indicated they received the 3-kDa ultrafiltrate, fraction I2-6, 1 μM uric acid, or fraction I2-6 after uricase treatment, as marked. Mycelial extract and fraction I2-6 were diluted to 1 μM uric acid. The mycelial extract added represented low-MW material extracted from 9.3 mg (dry weight); the final uric acid content was 1 μM. Fraction I2-6 was also present at a final concentration of 1 μM uric acid. Uricase treatment was done under the conditions used for the uric acid assay (see Materials and Methods). Additional columns of the time-zero experiment (termed 1 h) represent <t>minicultures</t> grown from conidiospores that were treated for 1 h with either mycelial extract or 1 μM uric acid, filtered, and resuspended in fresh culture liquid prior to incubation in the microtiter plates. Total alkaloid content was assayed after 8 days of culture as described in Materials and Methods. All data are means with SD, n = 8 wells.
    Minicultures, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minicultures/product/Millipore
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    minicultures - by Bioz Stars, 2020-08
    85/100 stars
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    Stimulation of alkaloid biosynthesis of emerged-grown cultures by mycelial extract, fraction I2-6, or uric acid. Conidial suspensions in 300 μl of culture liquid were grown in microplate compartments. At the times indicated they received the 3-kDa ultrafiltrate, fraction I2-6, 1 μM uric acid, or fraction I2-6 after uricase treatment, as marked. Mycelial extract and fraction I2-6 were diluted to 1 μM uric acid. The mycelial extract added represented low-MW material extracted from 9.3 mg (dry weight); the final uric acid content was 1 μM. Fraction I2-6 was also present at a final concentration of 1 μM uric acid. Uricase treatment was done under the conditions used for the uric acid assay (see Materials and Methods). Additional columns of the time-zero experiment (termed 1 h) represent minicultures grown from conidiospores that were treated for 1 h with either mycelial extract or 1 μM uric acid, filtered, and resuspended in fresh culture liquid prior to incubation in the microtiter plates. Total alkaloid content was assayed after 8 days of culture as described in Materials and Methods. All data are means with SD, n = 8 wells.

    Journal: Applied and Environmental Microbiology

    Article Title: Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus †

    doi: 10.1128/AEM.68.4.1524-1533.2002

    Figure Lengend Snippet: Stimulation of alkaloid biosynthesis of emerged-grown cultures by mycelial extract, fraction I2-6, or uric acid. Conidial suspensions in 300 μl of culture liquid were grown in microplate compartments. At the times indicated they received the 3-kDa ultrafiltrate, fraction I2-6, 1 μM uric acid, or fraction I2-6 after uricase treatment, as marked. Mycelial extract and fraction I2-6 were diluted to 1 μM uric acid. The mycelial extract added represented low-MW material extracted from 9.3 mg (dry weight); the final uric acid content was 1 μM. Fraction I2-6 was also present at a final concentration of 1 μM uric acid. Uricase treatment was done under the conditions used for the uric acid assay (see Materials and Methods). Additional columns of the time-zero experiment (termed 1 h) represent minicultures grown from conidiospores that were treated for 1 h with either mycelial extract or 1 μM uric acid, filtered, and resuspended in fresh culture liquid prior to incubation in the microtiter plates. Total alkaloid content was assayed after 8 days of culture as described in Materials and Methods. All data are means with SD, n = 8 wells.

    Article Snippet: The minicultures that had developed during 7 days of emerged growth at 24°C were filtered by suction (microplate handler Event; Millipore), and 100 μl of the filtrates was transferred to a heat-resistant microplate (PS-type; Greiner, Hamburg, Germany) for the colorimetric determination of external benzodiazepine alkaloid content.

    Techniques: Concentration Assay, Uric Acid Assay, Incubation