minicolumns  (Qiagen)


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    Structured Review

    Qiagen minicolumns
    The acidic domain of R contacts both TBP and TFIID in vitro. A. Schematic representation of the R protein. B. Schematic representation of the R mutant proteins tested for their capacity to bind TBP or TFIIB. Each variant has been assigned a recognition number (1–5). C. The R mutant proteins presented in B were translated in vitro in the presence of [ 35 S] methionine. Aliquots of the lysate extracts were either loaded directly onto SDS PAGE (lanes 1–5) or loaded onto <t>minicolumns</t> of GST- or GSThllB-agarose beads (lanes 6–15), nickel-NTA-agarose, or HisIID-nickel-NTA-agarose beads (lanes 16–25). The eluates were loaded onto SDS-PAGE. The gels were then autoradiographed. The mutant proteins used in each lane are indicated by their corresponding number. M indicates the lanes that correspond to the molecular weight markers.
    Minicolumns, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    Images

    1) Product Images from "The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region"

    Article Title: The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region

    Journal: Gene Expression

    doi:

    The acidic domain of R contacts both TBP and TFIID in vitro. A. Schematic representation of the R protein. B. Schematic representation of the R mutant proteins tested for their capacity to bind TBP or TFIIB. Each variant has been assigned a recognition number (1–5). C. The R mutant proteins presented in B were translated in vitro in the presence of [ 35 S] methionine. Aliquots of the lysate extracts were either loaded directly onto SDS PAGE (lanes 1–5) or loaded onto minicolumns of GST- or GSThllB-agarose beads (lanes 6–15), nickel-NTA-agarose, or HisIID-nickel-NTA-agarose beads (lanes 16–25). The eluates were loaded onto SDS-PAGE. The gels were then autoradiographed. The mutant proteins used in each lane are indicated by their corresponding number. M indicates the lanes that correspond to the molecular weight markers.
    Figure Legend Snippet: The acidic domain of R contacts both TBP and TFIID in vitro. A. Schematic representation of the R protein. B. Schematic representation of the R mutant proteins tested for their capacity to bind TBP or TFIIB. Each variant has been assigned a recognition number (1–5). C. The R mutant proteins presented in B were translated in vitro in the presence of [ 35 S] methionine. Aliquots of the lysate extracts were either loaded directly onto SDS PAGE (lanes 1–5) or loaded onto minicolumns of GST- or GSThllB-agarose beads (lanes 6–15), nickel-NTA-agarose, or HisIID-nickel-NTA-agarose beads (lanes 16–25). The eluates were loaded onto SDS-PAGE. The gels were then autoradiographed. The mutant proteins used in each lane are indicated by their corresponding number. M indicates the lanes that correspond to the molecular weight markers.

    Techniques Used: In Vitro, Mutagenesis, Variant Assay, SDS Page, Molecular Weight

    Related Articles

    Clone Assay:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl. .. For isolation of DNA from gels, the Qiaquick gel extraction kit (QIAGEN) was used.

    Centrifugation:

    Article Title: The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region
    Article Snippet: Cell debris was eliminated by centrifugation. .. The supernatant was loaded onto minicolumns of 80 μl Ni-NTA-agarose beads (Qiagen) previously equilibrated with the lysis buffer.

    Amplification:

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA). .. The RNA was reverse transcribed using Superscript First Strand (Invitrogen). cDNA was treated with ribonuclease H according to the manufacturer's protocol (Invitrogen). cDNA was amplified using Taqman gene expression assays for EST, adiponectin, leptin, TNF-α, and SOCS3.

    Chromatography:

    Article Title: The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region
    Article Snippet: Paragraph title: hTBP affinity column chromatography ... The supernatant was loaded onto minicolumns of 80 μl Ni-NTA-agarose beads (Qiagen) previously equilibrated with the lysis buffer.

    Homogenization:

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: RNA was extracted by mechanical homogenization using TRIzol (Invitrogen, Carlsbad, CA). .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA).

    Isolation:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: Lactococcal plasmid DNA was isolated from a 50-ml culture using a modified alkaline lysis method ( ) with an addition of lysozyme (30 mg ml−1 ) to the resuspension buffer. .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl.

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA). .. RNA was treated with deoxyribonuclease I according to the manufacturer's protocol (Invitrogen).

    Gel Extraction:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl. .. For isolation of DNA from gels, the Qiaquick gel extraction kit (QIAGEN) was used.

    Purification:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl. .. For isolation of DNA from gels, the Qiaquick gel extraction kit (QIAGEN) was used.

    Real-time Polymerase Chain Reaction:

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: The expression of adipose tissue genes was measured using real-time PCR as previously described ( ). .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA).

    Polymerase Chain Reaction:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl. .. For isolation of DNA from gels, the Qiaquick gel extraction kit (QIAGEN) was used.

    Selection:

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: These modified criteria were used to ensure the selection of subjects with impaired glucose homeostasis as part of their metabolic syndrome. .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA).

    Affinity Column:

    Article Title: The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region
    Article Snippet: Paragraph title: hTBP affinity column chromatography ... The supernatant was loaded onto minicolumns of 80 μl Ni-NTA-agarose beads (Qiagen) previously equilibrated with the lysis buffer.

    Expressing:

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: The expression of adipose tissue genes was measured using real-time PCR as previously described ( ). .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA).

    Alkaline Lysis:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: Lactococcal plasmid DNA was isolated from a 50-ml culture using a modified alkaline lysis method ( ) with an addition of lysozyme (30 mg ml−1 ) to the resuspension buffer. .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl.

    Modification:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: Lactococcal plasmid DNA was isolated from a 50-ml culture using a modified alkaline lysis method ( ) with an addition of lysozyme (30 mg ml−1 ) to the resuspension buffer. .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl.

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: These modified criteria were used to ensure the selection of subjects with impaired glucose homeostasis as part of their metabolic syndrome. .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA).

    Lysis:

    Article Title: The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region
    Article Snippet: .. The supernatant was loaded onto minicolumns of 80 μl Ni-NTA-agarose beads (Qiagen) previously equilibrated with the lysis buffer. ..

    Transformation Assay:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: For transformation of L. lactis , the procedure of Holo and Nes ( ) was utilized. .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl.

    Sampling:

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: After a screening visit, eligible subjects returned after an overnight 12 h fast for a baseline visit that included blood sampling, standard 2-h, 75 g oral glucose tolerance test, single-slice abdominal computed tomography scan , and abdominal sc fat biopsy. .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA).

    Computed Tomography:

    Article Title: Estrogen Sulfotransferase Is Expressed in Subcutaneous Adipose Tissue of Obese Humans in Association with TNF-? and SOCS3
    Article Snippet: After a screening visit, eligible subjects returned after an overnight 12 h fast for a baseline visit that included blood sampling, standard 2-h, 75 g oral glucose tolerance test, single-slice abdominal computed tomography scan , and abdominal sc fat biopsy. .. Cleared lysates were mixed with 1 volume of 70% ethanol and applied to RNeasy minicolumns for RNA isolation according to the manufacturer's protocol (QIAGEN, Valencia, CA).

    Plasmid Preparation:

    Article Title: Identification of Endopeptidase Genes from the Genomic Sequence of Lactobacillus helveticus CNRZ32 and the Role of These Genes in Hydrolysis of Model Bitter Peptides
    Article Snippet: Lactococcal plasmid DNA was isolated from a 50-ml culture using a modified alkaline lysis method ( ) with an addition of lysozyme (30 mg ml−1 ) to the resuspension buffer. .. DNA used for cloning was further purified by passing it through minicolumns from a Qiaquick PCR purification kit (QIAGEN, Valencia, CA) and was resuspended in a final volume of 30 to 50 μl.

    Article Title: The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region
    Article Snippet: hTBP containing 6 additional N-terminal his-tidines was expressed in E. coli B121 (DE3), from plasmid pET8cHisIID. .. The supernatant was loaded onto minicolumns of 80 μl Ni-NTA-agarose beads (Qiagen) previously equilibrated with the lysis buffer.

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  • News
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  • 93
    Qiagen minicolumns
    The acidic domain of R contacts both TBP and TFIID in vitro. A. Schematic representation of the R protein. B. Schematic representation of the R mutant proteins tested for their capacity to bind TBP or TFIIB. Each variant has been assigned a recognition number (1–5). C. The R mutant proteins presented in B were translated in vitro in the presence of [ 35 S] methionine. Aliquots of the lysate extracts were either loaded directly onto SDS PAGE (lanes 1–5) or loaded onto <t>minicolumns</t> of GST- or GSThllB-agarose beads (lanes 6–15), nickel-NTA-agarose, or HisIID-nickel-NTA-agarose beads (lanes 16–25). The eluates were loaded onto SDS-PAGE. The gels were then autoradiographed. The mutant proteins used in each lane are indicated by their corresponding number. M indicates the lanes that correspond to the molecular weight markers.
    Minicolumns, supplied by Qiagen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/minicolumns/product/Qiagen
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    minicolumns - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    85
    Qiagen qiagen rna minicolumns
    Expression of mefE (A) and mel (B). <t>RNA</t> was isolated from mid-exponential cultures using the <t>QIAGEN</t> RNeasy minipreps. Three replicates were performed for each strain on duplicate and independent RNA samples. The amount of target is normalized to a control
    Qiagen Rna Minicolumns, supplied by Qiagen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen rna minicolumns/product/Qiagen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiagen rna minicolumns - by Bioz Stars, 2020-04
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    96
    Qiagen rneasy minicolumns
    Expression of mefE (A) and mel (B). <t>RNA</t> was isolated from mid-exponential cultures using the <t>QIAGEN</t> RNeasy minipreps. Three replicates were performed for each strain on duplicate and independent RNA samples. The amount of target is normalized to a control
    Rneasy Minicolumns, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rneasy minicolumns/product/Qiagen
    Average 96 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    rneasy minicolumns - by Bioz Stars, 2020-04
    96/100 stars
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    Image Search Results


    The acidic domain of R contacts both TBP and TFIID in vitro. A. Schematic representation of the R protein. B. Schematic representation of the R mutant proteins tested for their capacity to bind TBP or TFIIB. Each variant has been assigned a recognition number (1–5). C. The R mutant proteins presented in B were translated in vitro in the presence of [ 35 S] methionine. Aliquots of the lysate extracts were either loaded directly onto SDS PAGE (lanes 1–5) or loaded onto minicolumns of GST- or GSThllB-agarose beads (lanes 6–15), nickel-NTA-agarose, or HisIID-nickel-NTA-agarose beads (lanes 16–25). The eluates were loaded onto SDS-PAGE. The gels were then autoradiographed. The mutant proteins used in each lane are indicated by their corresponding number. M indicates the lanes that correspond to the molecular weight markers.

    Journal: Gene Expression

    Article Title: The acidic activation domain of the Epstein-Barr virus transcription factor R interacts in vitro with both TBP and TFIIB and is cell-specifically potentiated by a proline-rich region

    doi:

    Figure Lengend Snippet: The acidic domain of R contacts both TBP and TFIID in vitro. A. Schematic representation of the R protein. B. Schematic representation of the R mutant proteins tested for their capacity to bind TBP or TFIIB. Each variant has been assigned a recognition number (1–5). C. The R mutant proteins presented in B were translated in vitro in the presence of [ 35 S] methionine. Aliquots of the lysate extracts were either loaded directly onto SDS PAGE (lanes 1–5) or loaded onto minicolumns of GST- or GSThllB-agarose beads (lanes 6–15), nickel-NTA-agarose, or HisIID-nickel-NTA-agarose beads (lanes 16–25). The eluates were loaded onto SDS-PAGE. The gels were then autoradiographed. The mutant proteins used in each lane are indicated by their corresponding number. M indicates the lanes that correspond to the molecular weight markers.

    Article Snippet: The supernatant was loaded onto minicolumns of 80 μl Ni-NTA-agarose beads (Qiagen) previously equilibrated with the lysis buffer.

    Techniques: In Vitro, Mutagenesis, Variant Assay, SDS Page, Molecular Weight

    Expression of mefE (A) and mel (B). RNA was isolated from mid-exponential cultures using the QIAGEN RNeasy minipreps. Three replicates were performed for each strain on duplicate and independent RNA samples. The amount of target is normalized to a control

    Journal:

    Article Title: Macrolide Efflux in Streptococcus pneumoniae Is Mediated by a Dual Efflux Pump (mel and mef) and Is Erythromycin Inducible

    doi: 10.1128/AAC.49.10.4203-4209.2005

    Figure Lengend Snippet: Expression of mefE (A) and mel (B). RNA was isolated from mid-exponential cultures using the QIAGEN RNeasy minipreps. Three replicates were performed for each strain on duplicate and independent RNA samples. The amount of target is normalized to a control

    Article Snippet: RNA was isolated using QIAGEN RNA minicolumns according to the manufacturer's protocol.

    Techniques: Expressing, Isolation