minelute 96 uf pcr purification plates  (Qiagen)


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    MinElute 96 UF PCR Purification Kit
    Description:
    For high throughput ultrafiltration purification of up to 15 μg PCR products 100 bp Kit contents Qiagen MinElute 96 UF PCR Purification Kit 20 to 30L Elution Volume 150L Binding Capacity 100 bp Fragment Size DNA oligonucleotides Sample 96 well Plate Format Ultrafiltration Technology Manual Automated Processing For High throughput Ultrafiltration Purification of up to 15μg PCR Products 100 bp Includes 4 MinElute 96 UF PCR Purification Plates Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries
    Catalog Number:
    28051
    Price:
    294
    Category:
    MinElute 96 UF PCR Purification Kit
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    Structured Review

    Qiagen minelute 96 uf pcr purification plates
    MinElute 96 UF PCR Purification Kit
    For high throughput ultrafiltration purification of up to 15 μg PCR products 100 bp Kit contents Qiagen MinElute 96 UF PCR Purification Kit 20 to 30L Elution Volume 150L Binding Capacity 100 bp Fragment Size DNA oligonucleotides Sample 96 well Plate Format Ultrafiltration Technology Manual Automated Processing For High throughput Ultrafiltration Purification of up to 15μg PCR Products 100 bp Includes 4 MinElute 96 UF PCR Purification Plates Benefits Very small elution volumes Fast procedure and easy handling High reproducible recoveries
    https://www.bioz.com/result/minelute 96 uf pcr purification plates/product/Qiagen
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    minelute 96 uf pcr purification plates - by Bioz Stars, 2020-03
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    Related Articles

    Methylation Sequencing:

    Article Title: Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines
    Article Snippet: Paragraph title: Bisulfite sequencing analysis ... PCR products were purified using the MinElute® 96 UF PCR Purification kit (#28051, Qiagen) and Sanger sequencied at Macrogen (Macrogen Europe).

    Clone Assay:

    Article Title: High-throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
    Article Snippet: If amplifying from plasmid DNA, the PCR reactions should subsequently be treated with Dpn I to remove template DNA (this step is not essential if the antibiotic resistance marker of the template DNA is different from the one carried on the cloning vector and in such circumstance can be avoided). .. Alternatively, for manual applications, a high throughput 96-well purification kit should be considered ( i.e. Qiagen MinElute 96 UF PCR Purification Kit).

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: Preparation of RNAi constructs Sequences of the target genes were amplified from cDNA clones by PCR using a universal forward primer and a specific reverse primer ( Supplementary Table S1 available at JXB online). .. The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to .

    Amplification:

    Article Title: Molecular Evidence for Metabolically Active Bacteria in the Atmosphere
    Article Snippet: Library Preparation and Sequencing 16S genes (rDNA) and transcripts (rRNA which was converted to cDNA) were amplified using bacterial/archaeal primers 515 forward (5′-GTGTGCCAGCMGCCGCGGTAA-3′) and 806 reverse (5′-GGACTACHVGGGTWTCTAAT-3′) ( ). .. PCR cycling conditions were as follows: initial denaturation at 98°C for 90 s followed by 35 cycles at 98°C for 20 s, 52°C for 30 s and 72°C for 30 s following by a final extension at 72°C for 10 min. Triplicate reactions were pooled, cleaned using the MinElute 96 UF PCR Purification Kit (QIAGEN), and eluted in 20 μl elution buffer.

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: One hundred and fifty ng of pooled DNA was digested using Sty I or Nsp I, ligated to appropriate adaptors and amplified using a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA) with 3 min 94o C, 30 cycles of 30 sec 94o C, 45 sec 60o C, 15 sec at 68o C and a final 7 min 68o C extension. .. PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated.

    Article Title: High-throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
    Article Snippet: Paragraph title: Pcr Amplification and Purification of PCR Products ... Alternatively, for manual applications, a high throughput 96-well purification kit should be considered ( i.e. Qiagen MinElute 96 UF PCR Purification Kit).

    Article Title: Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics
    Article Snippet: One microlitre of the extract was used for PCR amplification of the 18S ribosomal RNA gene. .. PCR products were purified using the MinElute 96 UF Kit (Qiagen) and sequenced using BigDye Terminator v3.1 and an ABI 3130 sequencer (Applied Biosystems).

    Article Title: Karyotype and reproduction mode of the rodent parasite Strongyloidesvenezuelensis
    Article Snippet: The D3 region of 28S rDNA was amplified using primers D3A-D3B (Nunn et al. ). .. PCR amplifications were carried out in 30 μ L reaction mixtures containing 15 μ L GoTaq Green Master Mix (Promega), 0·5 μ m of each primer, and 1 μ L of appropriately diluted nematode lysate under thermal-cycling conditions of 94 °C for 1 min, followed by 30 cycles of 94 °C for 30 s, 53 °C for 30 s and 72 °C for 1 min. PCR products were purified before sequencing using a MinElute 96 UF PCR purification plate (QIAGEN).

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: Preparation of RNAi constructs Sequences of the target genes were amplified from cDNA clones by PCR using a universal forward primer and a specific reverse primer ( Supplementary Table S1 available at JXB online). .. The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to .

    Article Title: Sequencing methods and datasets to improve functional interpretation of sleeping beauty mutagenesis screens
    Article Snippet: A normal genomic DNA sample, prepared and sequenced in the same manner, was used to identify any endogenous sites also amplified by the footprint detection method. .. PCR products were purified using the MinElute 96 UF PCR Purification Kit (Qiagen), enzymatically digested with Hpy CH4III (New England Biolabs), and separated by electrophoresis on 1.5% agarose gels.

    Article Title: Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae
    Article Snippet: PCR cycling conditions for DNA amplification were as follows: 98 °C for 30 sec, followed by 30 cycles of 98 °C for 9 s, 55 °C for 15 sec and 72 °C for 20 sec, followed by 10 min at 72 °C. .. To eliminate primer dimers, the PCR products were purified using a MinElute 96 UF PCR purification kit (Qiagen).

    Article Title: Humans differ in their personal microbial cloud
    Article Snippet: Each sample was amplified with a 2 step PCR prep method for Illumina sequencing of the V3-V4 region with 319F and 806R dual indexed primers including heterogeneity spacers to improve low plexity libraries, similar to methods used by Fadrosh and colleagues ( ). .. Triplicates were pooled and cleaned with Qiagen MinElute 96 UF PCR Purification kit prior to PCR2.

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: Ligated products were amplified in quadruplicate using 10 μM generic primer in PCR buffer II (Applied Biosystems) with 2.5 mM MgCl2 /2.5 mM deoxyribose nucleotide triphosphates (dNTPs)/10 units of AmpliTaq Gold polymerase (Applied Biosystems) under the following PCR conditions: 95 °C for 5 min, followed by 35 cycles (95 °C for 20 s, 59 °C for 15 s, and 72 °C for 15 s) and a final extension at 72 °C for 7 min. Fragments ranging in size from 250 to 1,000 bp were preferentially amplified under the conditions [ ]. .. PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations.

    Article Title: Life-long persistent viral infection alters the na?ve T-cell pool, impairing CD8 T-cell immunity in late life
    Article Snippet: The second PCR program was the same as the first, with 35 rounds of amplification. .. PCR products were purified with MinElute 96 UF PCR purification kits (Qiagen), and sequenced with 12 pmol of the internal degenerate Vβ5 sense primer, using an Applied Biosystems 3730KL DNA Analyzer at the University of Arizona Genomics Core (Tucson, AZ).

    Whole Genome Amplification:

    Article Title: Resequencing the susceptibility gene, ITGAM, identifies two functionally deleterious rare variants in systemic lupus erythematosus cases
    Article Snippet: The 454 sequencing and analysis Genomic DNA from 73 SLE patients meeting American College of Rheumatology criteria underwent whole-genome amplification (Qiagen Repli-G, Hilden, Germany). .. The PCR products were run on an agarose gel and purified by using the Qiagen MinElute 96 UF PCR Purification Kit.

    Polymerase Chain Reaction:

    Article Title: Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease
    Article Snippet: .. Thermal cycling parameters were 94 °C for 5 min; 35 cycles of 94 °C for 20 s, 50 °C for 20 s, and 72 °C for 30 s, followed by 72 °C for 5 min. PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). .. Libraries were sequenced (1 × 300 bases) using an Illumina MiSeq.

    Article Title: Molecular Evidence for Metabolically Active Bacteria in the Atmosphere
    Article Snippet: .. PCR cycling conditions were as follows: initial denaturation at 98°C for 90 s followed by 35 cycles at 98°C for 20 s, 52°C for 30 s and 72°C for 30 s following by a final extension at 72°C for 10 min. Triplicate reactions were pooled, cleaned using the MinElute 96 UF PCR Purification Kit (QIAGEN), and eluted in 20 μl elution buffer. .. The cleaned PCR products were quantitated using a Qubit 2.0 Fluorometer (Invitrogen, Life Technologies Corporation) and combined in equal molar concentrations.

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: .. PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated. .. Forty μg of PCR product was digested for 35 min at 37o C with 0.04 unit/μl DNase I to produce 30–100 bp fragments which were end-labeled using terminal deoxynucleotidyl transferase and biotinylated dideoxynucleotides and hybridized to the appropriate 500k array ( Sty I or Nsp I arrays) (Affymetrix, Santa Clara, CA).

    Article Title: High-throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
    Article Snippet: .. Alternatively, for manual applications, a high throughput 96-well purification kit should be considered ( i.e. Qiagen MinElute 96 UF PCR Purification Kit). .. KOD Hot Start DNA Polymerase Kit (Novagen #71086) Template DNA (genomic DNA at 10 ng/μl, plasmid DNA at 0.5 ng/μl, cDNA etc) Sense and anti-sense primer (5 μM each) assembled in 384 or 96 well plates Dimethyl Sulfoxide (DMSO) Restriction enzyme Dpn I (New England Biolabs): 20mM Tris-Acetate, pH 7.9 AMPure XP PCR purification kit (Beckman Coulter ) 96 well PCR plates (Eppendorf 951020401) 384 well PCR plates (Eppendorf 951020702) Adhesive foil for microplates (VWR # 60941-076) 96 well silicone sealing mat (Axygen CM-96-RD) Thermal cycler (Eppendorf Mastercycler , Eppendorf Mastercycler ep384 , or similar) Liquid handling robot E-gel 96 2% (w/v)agarose (Invitrogen G7008-02) Mother E-Base™ (Invitrogen EB-M03) We have found that 96- and 384-well plates can become misshapen following repeated heating and cooling (as in a PCR amplification), or after freezing.

    Article Title: Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics
    Article Snippet: .. PCR products were purified using the MinElute 96 UF Kit (Qiagen) and sequenced using BigDye Terminator v3.1 and an ABI 3130 sequencer (Applied Biosystems). ..

    Article Title: Karyotype and reproduction mode of the rodent parasite Strongyloidesvenezuelensis
    Article Snippet: .. PCR amplifications were carried out in 30 μ L reaction mixtures containing 15 μ L GoTaq Green Master Mix (Promega), 0·5 μ m of each primer, and 1 μ L of appropriately diluted nematode lysate under thermal-cycling conditions of 94 °C for 1 min, followed by 30 cycles of 94 °C for 30 s, 53 °C for 30 s and 72 °C for 1 min. PCR products were purified before sequencing using a MinElute 96 UF PCR purification plate (QIAGEN). .. DNA sequencing was performed using the BigDye Terminator 3.1 kit and ABI PRISM 3700 or 3130 Genetic Analyzer (Applied Biosystems).

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: .. The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to . .. The ligation reactions were used for transformation of chemically competent Escherichia coli TOP10 cells (Invitrogen, Karlsruhe, Germany), and spread on LB medium containing kanamycin (50 μg ml−1 ).

    Article Title: Sequencing methods and datasets to improve functional interpretation of sleeping beauty mutagenesis screens
    Article Snippet: .. PCR products were purified using the MinElute 96 UF PCR Purification Kit (Qiagen), enzymatically digested with Hpy CH4III (New England Biolabs), and separated by electrophoresis on 1.5% agarose gels. .. Construction of transposon standard plasmids Twenty PCR primers were designed to bind genomic DNA sequences adjacent to previously identified transposon insertion sites.

    Article Title: Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae
    Article Snippet: .. To eliminate primer dimers, the PCR products were purified using a MinElute 96 UF PCR purification kit (Qiagen). ..

    Article Title: Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines
    Article Snippet: .. PCR products were purified using the MinElute® 96 UF PCR Purification kit (#28051, Qiagen) and Sanger sequencied at Macrogen (Macrogen Europe). .. Sequence alignment and quantification of methylation was performed using ESME (Epigenomics Inc.).

    Article Title: Humans differ in their personal microbial cloud
    Article Snippet: .. Triplicates were pooled and cleaned with Qiagen MinElute 96 UF PCR Purification kit prior to PCR2. .. PCR2 contained 6.75 µL PCR-grade water, 0.25 µL Phusion HS II polymerase (2 U/µL), 5 µL 5x HF buffer, 0.5 µL dNTPs, 1.25 µL of forward and reverse primers (10 µM each) with Illumina adapter and index sequences, and 10 µL template from cleaned PCR1 products.

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: .. PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. A 10K genotyping assay kit fragmentation reagent (Affymetrix) was used to digest 20 μg of DNA, which was then labeled with 30 U/μL terminal deoxynucleotidyl transferase and 5 mM DNA labeling reagent (Affymetrix 10K genotyping assay kit).

    Article Title: Genome-wide variation in the pinewood nematode Bursaphelenchus xylophilus and its relationship with pathogenic traits
    Article Snippet: .. PCR products were purified using a MinElute96 UF PCR Purification Kit (Qiagen) and sequenced in both directions using a BigDye Terminator Sequencing Kit v.3.1 (Applied Biosystems) on a DNA multi-capillary sequencer (Model 3130XL genetic analyser; Applied Biosystems). ..

    Article Title: Life-long persistent viral infection alters the na?ve T-cell pool, impairing CD8 T-cell immunity in late life
    Article Snippet: .. PCR products were purified with MinElute 96 UF PCR purification kits (Qiagen), and sequenced with 12 pmol of the internal degenerate Vβ5 sense primer, using an Applied Biosystems 3730KL DNA Analyzer at the University of Arizona Genomics Core (Tucson, AZ). .. OVA257-264 -specific CD8+ TCRβ clonotypes were characterized by sequentially aligning each TCRβ sequence with the Vβ5.1 or 5.2 (TRBV12 in IMGT nomenclature) gene and then the best-match Jβ gene, using the IMGT reference alleles for the Mus musculus TRB genes ( ).

    Article Title: Resequencing the susceptibility gene, ITGAM, identifies two functionally deleterious rare variants in systemic lupus erythematosus cases
    Article Snippet: .. The PCR products were run on an agarose gel and purified by using the Qiagen MinElute 96 UF PCR Purification Kit. .. Seven amplicons greater than 1.5 kb were pooled and sheared to fragments of 500 to 800 bp on the Covaris E210.

    Construct:

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: Paragraph title: Preparation of RNAi constructs ... The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to .

    Electrophoresis:

    Article Title: Sequencing methods and datasets to improve functional interpretation of sleeping beauty mutagenesis screens
    Article Snippet: .. PCR products were purified using the MinElute 96 UF PCR Purification Kit (Qiagen), enzymatically digested with Hpy CH4III (New England Biolabs), and separated by electrophoresis on 1.5% agarose gels. .. Construction of transposon standard plasmids Twenty PCR primers were designed to bind genomic DNA sequences adjacent to previously identified transposon insertion sites.

    Microarray:

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. After undergoing heat inactivation at 95 °C for 10 min, samples were injected into microarray cartridges and hybridized overnight.

    Incubation:

    Article Title: Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics
    Article Snippet: Individual nematodes were transferred to a 10-µL aliquot of the lysis buffer and incubated at 60°C for 20 min followed by 95°C for 10 min. DNA extraction from cestodes was performed using the QIAamp DNA Mini Kit according to the manufacturer’s instructions (Qiagen). .. PCR products were purified using the MinElute 96 UF Kit (Qiagen) and sequenced using BigDye Terminator v3.1 and an ABI 3130 sequencer (Applied Biosystems).

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: Digested DNA was then incubated with a 0.25 M Xba I adaptor (Affymetrix) and DNA ligase (New England Biolabs) in standard ligation buffer for 2 h at 16 °C followed by heat inactivation for 20 min at 70 °C. .. PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations.

    Transformation Assay:

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to . .. The ligation reactions were used for transformation of chemically competent Escherichia coli TOP10 cells (Invitrogen, Karlsruhe, Germany), and spread on LB medium containing kanamycin (50 μg ml−1 ).

    Hybridization:

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: Hybridization probes were prepared with precautions to avoid contamination, as described (Affymetrix assays 500k ( )). .. PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated.

    Ligation:

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to . .. The ligation reactions were used for transformation of chemically competent Escherichia coli TOP10 cells (Invitrogen, Karlsruhe, Germany), and spread on LB medium containing kanamycin (50 μg ml−1 ).

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: Digested DNA was then incubated with a 0.25 M Xba I adaptor (Affymetrix) and DNA ligase (New England Biolabs) in standard ligation buffer for 2 h at 16 °C followed by heat inactivation for 20 min at 70 °C. .. PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations.

    Mapping Assay:

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated. .. Arrays were stained and washed as described (Affymetrix Genechip Mapping Assay Manual) using immunopure strepavidin (Pierce, Milwaukee, WI), biotinylated antistreptavidin antibody (Vector Labs, Burlingame, CA) and R-phycoerythrin strepavidin (Molecular Probes, Eugene, OR).

    DNA Sequencing:

    Article Title: Karyotype and reproduction mode of the rodent parasite Strongyloidesvenezuelensis
    Article Snippet: Paragraph title: PCR conditions and DNA sequencing ... PCR amplifications were carried out in 30 μ L reaction mixtures containing 15 μ L GoTaq Green Master Mix (Promega), 0·5 μ m of each primer, and 1 μ L of appropriately diluted nematode lysate under thermal-cycling conditions of 94 °C for 1 min, followed by 30 cycles of 94 °C for 30 s, 53 °C for 30 s and 72 °C for 1 min. PCR products were purified before sequencing using a MinElute 96 UF PCR purification plate (QIAGEN).

    DNA Labeling:

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. A 10K genotyping assay kit fragmentation reagent (Affymetrix) was used to digest 20 μg of DNA, which was then labeled with 30 U/μL terminal deoxynucleotidyl transferase and 5 mM DNA labeling reagent (Affymetrix 10K genotyping assay kit).

    Sequencing:

    Article Title: Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease
    Article Snippet: Paragraph title: 16S rRNA gene library construction and sequencing ... Thermal cycling parameters were 94 °C for 5 min; 35 cycles of 94 °C for 20 s, 50 °C for 20 s, and 72 °C for 30 s, followed by 72 °C for 5 min. PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA).

    Article Title: Molecular Evidence for Metabolically Active Bacteria in the Atmosphere
    Article Snippet: Paragraph title: Library Preparation and Sequencing ... PCR cycling conditions were as follows: initial denaturation at 98°C for 90 s followed by 35 cycles at 98°C for 20 s, 52°C for 30 s and 72°C for 30 s following by a final extension at 72°C for 10 min. Triplicate reactions were pooled, cleaned using the MinElute 96 UF PCR Purification Kit (QIAGEN), and eluted in 20 μl elution buffer.

    Article Title: Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics
    Article Snippet: Paragraph title: DNA extraction, sequencing from individual parasites and phylogenetic analyses ... PCR products were purified using the MinElute 96 UF Kit (Qiagen) and sequenced using BigDye Terminator v3.1 and an ABI 3130 sequencer (Applied Biosystems).

    Article Title: Karyotype and reproduction mode of the rodent parasite Strongyloidesvenezuelensis
    Article Snippet: .. PCR amplifications were carried out in 30 μ L reaction mixtures containing 15 μ L GoTaq Green Master Mix (Promega), 0·5 μ m of each primer, and 1 μ L of appropriately diluted nematode lysate under thermal-cycling conditions of 94 °C for 1 min, followed by 30 cycles of 94 °C for 30 s, 53 °C for 30 s and 72 °C for 1 min. PCR products were purified before sequencing using a MinElute 96 UF PCR purification plate (QIAGEN). .. DNA sequencing was performed using the BigDye Terminator 3.1 kit and ABI PRISM 3700 or 3130 Genetic Analyzer (Applied Biosystems).

    Article Title: Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines
    Article Snippet: PCR products were purified using the MinElute® 96 UF PCR Purification kit (#28051, Qiagen) and Sanger sequencied at Macrogen (Macrogen Europe). .. Sequence alignment and quantification of methylation was performed using ESME (Epigenomics Inc.).

    Article Title: Humans differ in their personal microbial cloud
    Article Snippet: Each sample was amplified with a 2 step PCR prep method for Illumina sequencing of the V3-V4 region with 319F and 806R dual indexed primers including heterogeneity spacers to improve low plexity libraries, similar to methods used by Fadrosh and colleagues ( ). .. Triplicates were pooled and cleaned with Qiagen MinElute 96 UF PCR Purification kit prior to PCR2.

    Article Title: Genome-wide variation in the pinewood nematode Bursaphelenchus xylophilus and its relationship with pathogenic traits
    Article Snippet: .. PCR products were purified using a MinElute96 UF PCR Purification Kit (Qiagen) and sequenced in both directions using a BigDye Terminator Sequencing Kit v.3.1 (Applied Biosystems) on a DNA multi-capillary sequencer (Model 3130XL genetic analyser; Applied Biosystems). ..

    Article Title: Resequencing the susceptibility gene, ITGAM, identifies two functionally deleterious rare variants in systemic lupus erythematosus cases
    Article Snippet: Paragraph title: The 454 sequencing and analysis ... The PCR products were run on an agarose gel and purified by using the Qiagen MinElute 96 UF PCR Purification Kit.

    Injection:

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. After undergoing heat inactivation at 95 °C for 10 min, samples were injected into microarray cartridges and hybridized overnight.

    DNA Extraction:

    Article Title: Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease
    Article Snippet: 16S rRNA gene library construction and sequencing Total DNA was extracted from rectal swabs using the PowerSoil DNA Isolation Kit (MO BIO Laboratories, Carlsbad, CA, USA), and a 30-s bead beating step using a Mini-Beadbeater-16 (BioSpec Products, Bartlesville, OK, USA). .. Thermal cycling parameters were 94 °C for 5 min; 35 cycles of 94 °C for 20 s, 50 °C for 20 s, and 72 °C for 30 s, followed by 72 °C for 5 min. PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA).

    Article Title: Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics
    Article Snippet: Paragraph title: DNA extraction, sequencing from individual parasites and phylogenetic analyses ... PCR products were purified using the MinElute 96 UF Kit (Qiagen) and sequenced using BigDye Terminator v3.1 and an ABI 3130 sequencer (Applied Biosystems).

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to . .. One colony of each cloning reaction was used for plasmid DNA isolation with the QIAprep® Miniprep Kit (Qiagen).

    Article Title: Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae
    Article Snippet: Since the larvae were too small to remove the intestinal tract, whole single larvae were used for DNA extraction (DNeasy 96 blood & tissue kit, Qiagen). .. To eliminate primer dimers, the PCR products were purified using a MinElute 96 UF PCR purification kit (Qiagen).

    Marker:

    Article Title: High-throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
    Article Snippet: If amplifying from plasmid DNA, the PCR reactions should subsequently be treated with Dpn I to remove template DNA (this step is not essential if the antibiotic resistance marker of the template DNA is different from the one carried on the cloning vector and in such circumstance can be avoided). .. Alternatively, for manual applications, a high throughput 96-well purification kit should be considered ( i.e. Qiagen MinElute 96 UF PCR Purification Kit).

    Fluorescence:

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated. .. Arrays were scanned and fluorescence intensities quantitated using an Affymetrix array scanner as described ( ).

    Methylation:

    Article Title: Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines
    Article Snippet: PCR products were purified using the MinElute® 96 UF PCR Purification kit (#28051, Qiagen) and Sanger sequencied at Macrogen (Macrogen Europe). .. Sequence alignment and quantification of methylation was performed using ESME (Epigenomics Inc.).

    Size-exclusion Chromatography:

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: One hundred and fifty ng of pooled DNA was digested using Sty I or Nsp I, ligated to appropriate adaptors and amplified using a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA) with 3 min 94o C, 30 cycles of 30 sec 94o C, 45 sec 60o C, 15 sec at 68o C and a final 7 min 68o C extension. .. PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated.

    Article Title: Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae
    Article Snippet: PCR cycling conditions for DNA amplification were as follows: 98 °C for 30 sec, followed by 30 cycles of 98 °C for 9 s, 55 °C for 15 sec and 72 °C for 20 sec, followed by 10 min at 72 °C. .. To eliminate primer dimers, the PCR products were purified using a MinElute 96 UF PCR purification kit (Qiagen).

    Article Title: Life-long persistent viral infection alters the na?ve T-cell pool, impairing CD8 T-cell immunity in late life
    Article Snippet: The PCR cycling program utilized 5 min at 95°C; 40 cycles of 20 sec at 95°C, 20 sec at 56°C, and 45 sec at 72°C; ending with 5 min at 72°C. .. PCR products were purified with MinElute 96 UF PCR purification kits (Qiagen), and sequenced with 12 pmol of the internal degenerate Vβ5 sense primer, using an Applied Biosystems 3730KL DNA Analyzer at the University of Arizona Genomics Core (Tucson, AZ).

    Labeling:

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. A 10K genotyping assay kit fragmentation reagent (Affymetrix) was used to digest 20 μg of DNA, which was then labeled with 30 U/μL terminal deoxynucleotidyl transferase and 5 mM DNA labeling reagent (Affymetrix 10K genotyping assay kit).

    Purification:

    Article Title: Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease
    Article Snippet: .. Thermal cycling parameters were 94 °C for 5 min; 35 cycles of 94 °C for 20 s, 50 °C for 20 s, and 72 °C for 30 s, followed by 72 °C for 5 min. PCR products were purified using a MinElute 96 UF PCR Purification Kit (Qiagen, Valencia, CA, USA). .. Libraries were sequenced (1 × 300 bases) using an Illumina MiSeq.

    Article Title: Molecular Evidence for Metabolically Active Bacteria in the Atmosphere
    Article Snippet: .. PCR cycling conditions were as follows: initial denaturation at 98°C for 90 s followed by 35 cycles at 98°C for 20 s, 52°C for 30 s and 72°C for 30 s following by a final extension at 72°C for 10 min. Triplicate reactions were pooled, cleaned using the MinElute 96 UF PCR Purification Kit (QIAGEN), and eluted in 20 μl elution buffer. .. The cleaned PCR products were quantitated using a Qubit 2.0 Fluorometer (Invitrogen, Life Technologies Corporation) and combined in equal molar concentrations.

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: .. PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated. .. Forty μg of PCR product was digested for 35 min at 37o C with 0.04 unit/μl DNase I to produce 30–100 bp fragments which were end-labeled using terminal deoxynucleotidyl transferase and biotinylated dideoxynucleotides and hybridized to the appropriate 500k array ( Sty I or Nsp I arrays) (Affymetrix, Santa Clara, CA).

    Article Title: High-throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
    Article Snippet: .. Alternatively, for manual applications, a high throughput 96-well purification kit should be considered ( i.e. Qiagen MinElute 96 UF PCR Purification Kit). .. KOD Hot Start DNA Polymerase Kit (Novagen #71086) Template DNA (genomic DNA at 10 ng/μl, plasmid DNA at 0.5 ng/μl, cDNA etc) Sense and anti-sense primer (5 μM each) assembled in 384 or 96 well plates Dimethyl Sulfoxide (DMSO) Restriction enzyme Dpn I (New England Biolabs): 20mM Tris-Acetate, pH 7.9 AMPure XP PCR purification kit (Beckman Coulter ) 96 well PCR plates (Eppendorf 951020401) 384 well PCR plates (Eppendorf 951020702) Adhesive foil for microplates (VWR # 60941-076) 96 well silicone sealing mat (Axygen CM-96-RD) Thermal cycler (Eppendorf Mastercycler , Eppendorf Mastercycler ep384 , or similar) Liquid handling robot E-gel 96 2% (w/v)agarose (Invitrogen G7008-02) Mother E-Base™ (Invitrogen EB-M03) We have found that 96- and 384-well plates can become misshapen following repeated heating and cooling (as in a PCR amplification), or after freezing.

    Article Title: Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics
    Article Snippet: .. PCR products were purified using the MinElute 96 UF Kit (Qiagen) and sequenced using BigDye Terminator v3.1 and an ABI 3130 sequencer (Applied Biosystems). ..

    Article Title: Karyotype and reproduction mode of the rodent parasite Strongyloidesvenezuelensis
    Article Snippet: .. PCR amplifications were carried out in 30 μ L reaction mixtures containing 15 μ L GoTaq Green Master Mix (Promega), 0·5 μ m of each primer, and 1 μ L of appropriately diluted nematode lysate under thermal-cycling conditions of 94 °C for 1 min, followed by 30 cycles of 94 °C for 30 s, 53 °C for 30 s and 72 °C for 1 min. PCR products were purified before sequencing using a MinElute 96 UF PCR purification plate (QIAGEN). .. DNA sequencing was performed using the BigDye Terminator 3.1 kit and ABI PRISM 3700 or 3130 Genetic Analyzer (Applied Biosystems).

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: .. The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to . .. The ligation reactions were used for transformation of chemically competent Escherichia coli TOP10 cells (Invitrogen, Karlsruhe, Germany), and spread on LB medium containing kanamycin (50 μg ml−1 ).

    Article Title: Sequencing methods and datasets to improve functional interpretation of sleeping beauty mutagenesis screens
    Article Snippet: .. PCR products were purified using the MinElute 96 UF PCR Purification Kit (Qiagen), enzymatically digested with Hpy CH4III (New England Biolabs), and separated by electrophoresis on 1.5% agarose gels. .. Construction of transposon standard plasmids Twenty PCR primers were designed to bind genomic DNA sequences adjacent to previously identified transposon insertion sites.

    Article Title: Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae
    Article Snippet: .. To eliminate primer dimers, the PCR products were purified using a MinElute 96 UF PCR purification kit (Qiagen). ..

    Article Title: Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines
    Article Snippet: .. PCR products were purified using the MinElute® 96 UF PCR Purification kit (#28051, Qiagen) and Sanger sequencied at Macrogen (Macrogen Europe). .. Sequence alignment and quantification of methylation was performed using ESME (Epigenomics Inc.).

    Article Title: Humans differ in their personal microbial cloud
    Article Snippet: .. Triplicates were pooled and cleaned with Qiagen MinElute 96 UF PCR Purification kit prior to PCR2. .. PCR2 contained 6.75 µL PCR-grade water, 0.25 µL Phusion HS II polymerase (2 U/µL), 5 µL 5x HF buffer, 0.5 µL dNTPs, 1.25 µL of forward and reverse primers (10 µM each) with Illumina adapter and index sequences, and 10 µL template from cleaned PCR1 products.

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: .. PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. A 10K genotyping assay kit fragmentation reagent (Affymetrix) was used to digest 20 μg of DNA, which was then labeled with 30 U/μL terminal deoxynucleotidyl transferase and 5 mM DNA labeling reagent (Affymetrix 10K genotyping assay kit).

    Article Title: Genome-wide variation in the pinewood nematode Bursaphelenchus xylophilus and its relationship with pathogenic traits
    Article Snippet: .. PCR products were purified using a MinElute96 UF PCR Purification Kit (Qiagen) and sequenced in both directions using a BigDye Terminator Sequencing Kit v.3.1 (Applied Biosystems) on a DNA multi-capillary sequencer (Model 3130XL genetic analyser; Applied Biosystems). ..

    Article Title: Life-long persistent viral infection alters the na?ve T-cell pool, impairing CD8 T-cell immunity in late life
    Article Snippet: .. PCR products were purified with MinElute 96 UF PCR purification kits (Qiagen), and sequenced with 12 pmol of the internal degenerate Vβ5 sense primer, using an Applied Biosystems 3730KL DNA Analyzer at the University of Arizona Genomics Core (Tucson, AZ). .. OVA257-264 -specific CD8+ TCRβ clonotypes were characterized by sequentially aligning each TCRβ sequence with the Vβ5.1 or 5.2 (TRBV12 in IMGT nomenclature) gene and then the best-match Jβ gene, using the IMGT reference alleles for the Mus musculus TRB genes ( ).

    Article Title: Resequencing the susceptibility gene, ITGAM, identifies two functionally deleterious rare variants in systemic lupus erythematosus cases
    Article Snippet: .. The PCR products were run on an agarose gel and purified by using the Qiagen MinElute 96 UF PCR Purification Kit. .. Seven amplicons greater than 1.5 kb were pooled and sheared to fragments of 500 to 800 bp on the Covaris E210.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Life-long persistent viral infection alters the na?ve T-cell pool, impairing CD8 T-cell immunity in late life
    Article Snippet: Paragraph title: cDNA synthesis and RT-PCR ... PCR products were purified with MinElute 96 UF PCR purification kits (Qiagen), and sequenced with 12 pmol of the internal degenerate Vβ5 sense primer, using an Applied Biosystems 3730KL DNA Analyzer at the University of Arizona Genomics Core (Tucson, AZ).

    Staining:

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated. .. Arrays were stained and washed as described (Affymetrix Genechip Mapping Assay Manual) using immunopure strepavidin (Pierce, Milwaukee, WI), biotinylated antistreptavidin antibody (Vector Labs, Burlingame, CA) and R-phycoerythrin strepavidin (Molecular Probes, Eugene, OR).

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. Microarrays were washed in a fluidics station 450 (Affymetrix), followed by staining with streptavidin Avidin Phycoerythrin (Molecular Probes, Eugene, OR), and biotinylated antistreptavidin (Vector Lab, Burlingame, CA), followed by a final wash with SSPE buffer.

    Nested PCR:

    Article Title: Life-long persistent viral infection alters the na?ve T-cell pool, impairing CD8 T-cell immunity in late life
    Article Snippet: Vβ5 transcripts were amplified by nested PCR with the entire 5 μl cDNA reaction used for the first PCR reaction in a final 25 μl volume containing 1.25 U DreamTaq polymerase (Fisher Scientific) in the manufacturer's 1x Buffer with 200 μM each 2’-deoxynucleoside 5’-triphosphate (Fisher Scientific), and 100 nM external degenerate sense Vβ5 primer (5’-GGGGTTGTCCAGTCTCC-3’) and external antisense Vβ5 primer (5’-CCAGAAGGTAGCAGAGACCC-3’). .. PCR products were purified with MinElute 96 UF PCR purification kits (Qiagen), and sequenced with 12 pmol of the internal degenerate Vβ5 sense primer, using an Applied Biosystems 3730KL DNA Analyzer at the University of Arizona Genomics Core (Tucson, AZ).

    Plasmid Preparation:

    Article Title: Genome-Wide Association for Nicotine Dependence and Smoking Cessation Success in NIH Research Volunteers
    Article Snippet: PCR products were purified (MinEluteTM 96 UF kits, Qiagen, Valencia, CA) and quantitated. .. Arrays were stained and washed as described (Affymetrix Genechip Mapping Assay Manual) using immunopure strepavidin (Pierce, Milwaukee, WI), biotinylated antistreptavidin antibody (Vector Labs, Burlingame, CA) and R-phycoerythrin strepavidin (Molecular Probes, Eugene, OR).

    Article Title: High-throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
    Article Snippet: If amplifying from plasmid DNA, the PCR reactions should subsequently be treated with Dpn I to remove template DNA (this step is not essential if the antibiotic resistance marker of the template DNA is different from the one carried on the cloning vector and in such circumstance can be avoided). .. Alternatively, for manual applications, a high throughput 96-well purification kit should be considered ( i.e. Qiagen MinElute 96 UF PCR Purification Kit).

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: .. The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to . .. The ligation reactions were used for transformation of chemically competent Escherichia coli TOP10 cells (Invitrogen, Karlsruhe, Germany), and spread on LB medium containing kanamycin (50 μg ml−1 ).

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. Microarrays were washed in a fluidics station 450 (Affymetrix), followed by staining with streptavidin Avidin Phycoerythrin (Molecular Probes, Eugene, OR), and biotinylated antistreptavidin (Vector Lab, Burlingame, CA), followed by a final wash with SSPE buffer.

    Software:

    Article Title: A transient assay system for the assessment of cell-autonomous gene function in dehydration-stressed barley
    Article Snippet: Specific primers were designed by the software program Lasergene (DNASTAR, Madison, WI, USA). .. The resulting PCR fragments with an average length of ∼500 bp were purified by MinElute 96 UF PCR purification plates (Qiagen, Hilden, Germany) and ligated into the Swa I site of vector pIPKTA38 according to .

    SYBR Green Assay:

    Article Title: Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines
    Article Snippet: The PCR reaction contained 1x IQ SYBR green supermix (#170–8862, Biorad), 1μL of the bisulfite converted DNA and 5 nmol forward and reverse primer. .. PCR products were purified using the MinElute® 96 UF PCR Purification kit (#28051, Qiagen) and Sanger sequencied at Macrogen (Macrogen Europe).

    Avidin-Biotin Assay:

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. Microarrays were washed in a fluidics station 450 (Affymetrix), followed by staining with streptavidin Avidin Phycoerythrin (Molecular Probes, Eugene, OR), and biotinylated antistreptavidin (Vector Lab, Burlingame, CA), followed by a final wash with SSPE buffer.

    Agarose Gel Electrophoresis:

    Article Title: Resequencing the susceptibility gene, ITGAM, identifies two functionally deleterious rare variants in systemic lupus erythematosus cases
    Article Snippet: .. The PCR products were run on an agarose gel and purified by using the Qiagen MinElute 96 UF PCR Purification Kit. .. Seven amplicons greater than 1.5 kb were pooled and sheared to fragments of 500 to 800 bp on the Covaris E210.

    DNA Methylation Assay:

    Article Title: Methylation associated transcriptional repression of ELOVL5 in novel colorectal cancer cell lines
    Article Snippet: Bisulfite sequencing analysis Bisulfite conversion was performed on 200 ng of DNA using the EZ DNA methylation Gold kit and eluted in 15μL MQ water. .. PCR products were purified using the MinElute® 96 UF PCR Purification kit (#28051, Qiagen) and Sanger sequencied at Macrogen (Macrogen Europe).

    Genotyping Assay:

    Article Title: Identification of a novel splice-site mutation in the Lebercilin (LCA5) gene causing Leber congenital amaurosis
    Article Snippet: PCR products were purified with Qiagen MinElute 96 UF PCR Purification Kit and concentrated with a Qiagen PCR purification column (Qiagen,) according to the manufacturer's recommendations. .. A 10K genotyping assay kit fragmentation reagent (Affymetrix) was used to digest 20 μg of DNA, which was then labeled with 30 U/μL terminal deoxynucleotidyl transferase and 5 mM DNA labeling reagent (Affymetrix 10K genotyping assay kit).

    Lysis:

    Article Title: Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics
    Article Snippet: Individual nematodes were transferred to a 10-µL aliquot of the lysis buffer and incubated at 60°C for 20 min followed by 95°C for 10 min. DNA extraction from cestodes was performed using the QIAamp DNA Mini Kit according to the manufacturer’s instructions (Qiagen). .. PCR products were purified using the MinElute 96 UF Kit (Qiagen) and sequenced using BigDye Terminator v3.1 and an ABI 3130 sequencer (Applied Biosystems).

    High Throughput Screening Assay:

    Article Title: High-throughput Cloning and Expression of Integral Membrane Proteins in Escherichia coli
    Article Snippet: .. Alternatively, for manual applications, a high throughput 96-well purification kit should be considered ( i.e. Qiagen MinElute 96 UF PCR Purification Kit). .. KOD Hot Start DNA Polymerase Kit (Novagen #71086) Template DNA (genomic DNA at 10 ng/μl, plasmid DNA at 0.5 ng/μl, cDNA etc) Sense and anti-sense primer (5 μM each) assembled in 384 or 96 well plates Dimethyl Sulfoxide (DMSO) Restriction enzyme Dpn I (New England Biolabs): 20mM Tris-Acetate, pH 7.9 AMPure XP PCR purification kit (Beckman Coulter ) 96 well PCR plates (Eppendorf 951020401) 384 well PCR plates (Eppendorf 951020702) Adhesive foil for microplates (VWR # 60941-076) 96 well silicone sealing mat (Axygen CM-96-RD) Thermal cycler (Eppendorf Mastercycler , Eppendorf Mastercycler ep384 , or similar) Liquid handling robot E-gel 96 2% (w/v)agarose (Invitrogen G7008-02) Mother E-Base™ (Invitrogen EB-M03) We have found that 96- and 384-well plates can become misshapen following repeated heating and cooling (as in a PCR amplification), or after freezing.

    Gel Extraction:

    Article Title: Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae
    Article Snippet: To eliminate primer dimers, the PCR products were purified using a MinElute 96 UF PCR purification kit (Qiagen). .. 30 ng DNA per sample were pooled and a gel extraction was conducted (NucleoSpin gel and PCR clean-up kit, Macherey-Nagel).

    Variant Assay:

    Article Title: Genome-wide variation in the pinewood nematode Bursaphelenchus xylophilus and its relationship with pathogenic traits
    Article Snippet: Primers were designed from flanking regions of each variant using Primer3 v2.3.5 [ ]. .. PCR products were purified using a MinElute96 UF PCR Purification Kit (Qiagen) and sequenced in both directions using a BigDye Terminator Sequencing Kit v.3.1 (Applied Biosystems) on a DNA multi-capillary sequencer (Model 3130XL genetic analyser; Applied Biosystems).

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    Qiagen minelute 96 uf pcr purification plates
    Minelute 96 Uf Pcr Purification Plates, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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