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minion mk1b nanopore sequencing device min-101b  (Oxford Nanopore)
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Minion Mk1b Nanopore Sequencing Device Min 101b, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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minion mk1b nanopore sequencing device min-101b - by Bioz Stars, 2026-07
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minion sequencer min-101b  (Oxford Nanopore)
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Oxford Nanopore minion sequencer min-101b
Minion Sequencer Min 101b, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/min-101b/pm40348784-71-34-37?v=Oxford+Nanopore
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minion sequencer min-101b - by Bioz Stars, 2026-07
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minion mk1b sequencing device min-101b  (Oxford Nanopore)
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Oxford Nanopore minion mk1b sequencing device min-101b
( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
Minion Mk1b Sequencing Device Min 101b, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/min-101b/bio_rxiv__2025__04__03__647077-27-0-49?v=Oxford+Nanopore
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minion mk1b sequencing device min-101b - by Bioz Stars, 2026-07
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minion sequencing device oxford nanopore min-101b  (Oxford Nanopore)
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Oxford Nanopore minion sequencing device oxford nanopore min-101b
( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
Minion Sequencing Device Oxford Nanopore Min 101b, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/min-101b/pm40140852-149-20-23?v=Oxford+Nanopore
Average 90 stars, based on 1 article reviews
minion sequencing device oxford nanopore min-101b - by Bioz Stars, 2026-07
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minion min-101b sequencer  (Oxford Nanopore)
90
Oxford Nanopore minion min-101b sequencer
( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
Minion Min 101b Sequencer, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/min-101b/pm39907824-74-8-10?v=Oxford+Nanopore
Average 90 stars, based on 1 article reviews
minion min-101b sequencer - by Bioz Stars, 2026-07
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annotation isolated genomes  (Oxford Nanopore)
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( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
Annotation Isolated Genomes, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annotation isolated genomes - by Bioz Stars, 2026-07
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minion mk1b sequencer  (Oxford Nanopore)
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Oxford Nanopore minion mk1b sequencer
( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
Minion Mk1b Sequencer, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/min-101b/pmc11704337-329-21-24?v=Oxford+Nanopore
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minion mk1b sequencer - by Bioz Stars, 2026-07
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minion mk1b instrument  (Oxford Nanopore)
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Oxford Nanopore minion mk1b instrument
( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
Minion Mk1b Instrument, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/min-101b/pmc11720700-328-5-15?v=Oxford+Nanopore
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minion mk1b instrument - by Bioz Stars, 2026-07
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minion sequencer  (Oxford Nanopore)
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Oxford Nanopore minion sequencer
( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore <t>sequencing</t> using v10 chemistry native barcoding and sequenced on a <t>MinION</t> sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.
Minion Sequencer, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/min-101b/pm39702830-65-23-26?v=Oxford+Nanopore
Average 96 stars, based on 1 article reviews
minion sequencer - by Bioz Stars, 2026-07
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( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore sequencing using v10 chemistry native barcoding and sequenced on a MinION sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.

Journal: bioRxiv

Article Title: ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples

doi: 10.1101/2025.04.03.647077

Figure Lengend Snippet: ( A ) Chimeric read experiment schematic. UMI tags were appended to flanking regions of the degenerate barcode of a pool barcoded plasmids using two cycles of PCR with universal primer binding sites. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for nanopore sequencing using v10 chemistry native barcoding and sequenced on a MinION sequencer using an R9 flow cell. B ) Cluster sizes in reads of non-chimeric and chimeric UMI pairings for 1.5 × 10 6 (top panel) or 1.5 × 10 5 (bottom panel) input molecules following ConSeqUMI processing. Clusters containing chimeric UMIs are displayed in blue. Inset box plots summarize the data of the quantile plots. C ) Isolation of two transgenes from heterogeneous pools via PCR using UMIs as primer binding sites. Abridged cluster consensus sequences for each ConSeqUMI algorithm are shown below the template sequence region containing degenerate nucleotides, with the constant backbone regions in gray. Chromatograms display the results of Sanger sequencing of the resulting amplicons. D ) Plasmid pool experiment schematic. UMI tags were appended to variable regions of a pool of 14 pcDNA3.1-based plasmids using two cycles of PCR with universal primer binding sites on the pcDNA3.1 backbone. Following bead cleanup, amplicons were further amplified with additional PCR cycles using appended constant regions as primer binding sites. Amplicons were prepared for sequencing using v14 chemistry native barcoding and sequenced on a MinION sequencer using an R10 flow cell. E ) Replicate UMI-labeled plasmid pool samples were sequenced and clustered using ConSeqUMI. Primer-based UMI addition showed consistent cluster identity proportions across replicates per input amount.

Article Snippet: MinION Mk1B Sequencing Device (MIN-101B), Ligation Sequencing Kit (SQK-LSK109) with the Adapter Mix II Expansion (EXP-AMII001), Spot-ON Flow Cell, R9 Version (FLO-MIN106D), MinION Flow Cell, R10 Version (FLO-MIN114), Flongle Flow Cell, R10 Version (FLO-FLG114), Flongle Sequencing Expansion (EXP-FSE002), and the Native Barcoding Kit 96 v14 (SQK-NBD114.96) were obtained from Oxford Nanopore Technologies (Oxford, United Kingdom). dPTP (6H,8H-3,4-Dihydro-pyrimido(4,5-c)(1,2)oxazin-7-one-8-β-D-2’-deoxy-ribofuranoside-5’-triphosphate, Sodium salt) (NU-1119S) was obtained from Jena Bioscience (Jena, Germany).

Techniques: Binding Assay, Amplification, Nanopore Sequencing, Isolation, Sequencing, Plasmid Preparation, Labeling