Structured Review

Santa Cruz Biotechnology milk pbs t
Milk Pbs T, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/milk pbs t/product/Santa Cruz Biotechnology
Average 91 stars, based on 11 article reviews
Price from $9.99 to $1999.99
milk pbs t - by Bioz Stars, 2020-07
91/100 stars

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Western Blot:

Article Title: Uterine Dysfunction in Biglycan and Decorin Deficient Mice Leads to Dystocia during Parturition
Article Snippet: .. The membrane was stripped using Restore Western Blot stripping buffer (Thermo Scientific, Waltham, MA) for 10 min and blocked with 5% milk PBS-T for 20 min, then re-probed with anti-GAPDH antibody at a 1∶800 dilution (Santa Cruz Biotech, Santa Cruz, CA) as an internal standard. .. Immunoblots were digitally scanned and densitometrically analyzed using Gel-Pro Analyzer (Media Cybernetics, Bethesda, MD).

Incubation:

Article Title: Canine Degenerative Myelopathy: Biochemical characterization of superoxide dismutase 1 in the first naturally occurring non-human amyotrophic lateral sclerosis model
Article Snippet: .. Membranes were blocked in 5% milk PBS-T for 1 hour at room temperature and incubated with anti-SOD1 (FL-154) antibody (Santa Cruz) in 5% milk PBS-T overnight at 4°C. .. Following three washes, membranes were incubated with donkey anti-rabbit IgG, horseradish peroxidase-linked whole antibody (GE Healthcare) at 1:5000 in 5% milk PBS-T for 2 hours at room temperature.

Article Title: Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA
Article Snippet: .. SDS-PAGE-separated proteins were transferred onto nitrocellulose membranes using an iBlot gel transfer device (Invitrogen) set at P0 (20 V 1min, 23 V 4 min, 25 V 2 min) and blocked with 5% milk PBS-T (0.05% tween 20) before incubation with 1:1000 rabbit anti-FANCD2 (sc -28194; Santa Cruz Biotechnology) and 1:100 mouse anti-FANCI (sc -271316; Santa Cruz Biotechnology) or anti-V5 (66007.1-Ig; ProteinTech) for 60 minutes at room temperature, or overnight at 4 °C. .. Membranes were washed extensively with PBS-T, incubated with secondary infrared-labelled antibodies (Li-Cor) for 90 minutes at room temperature and then washed extensively again with PBS-T. Bands were visualised on an Odyssey CLx (Li-Cor) using the 700- or 800-nm channel.

Article Title: Preclinical Assessment of the Efficacy of Mycograb, a Human Recombinant Antibody against Fungal HSP90
Article Snippet: .. The separated proteins were transferred onto nitrocellulose membrane, blocked in 5% milk-PBS-T, washed and incubated with rabbit polyclonal anti-His horseradish peroxidase conjugate (Santa Cruz) at a 1:500 dilution in 5% milk-PBS-T, rewashed, and finally visualized by ECL developing reagents (Amersham Pharmacia). ..

Article Title: Molecular regulation of the hypothalamic-pituitary-adrenal axis in adult male guinea pigs after prenatal stress at different stages of gestation
Article Snippet: .. Membranes were then washed and incubated with a primary antibody in 5% skimmed milk PBS-T. Primary antibodies used were as follows: rabbit anti-MR (1 : 200; MCR, H-300, sc-11412, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-GR (1 : 500; H-300, sc-8992, Santa Cruz Biotechnology) and rabbit anti-tubulin (1 : 10 000; T-3526, Sigma-Aldrich Corp.). ..

Stripping Membranes:

Article Title: Biglycan and decorin differentially regulate signaling in the fetal membranes
Article Snippet: .. The membrane was stripped using Antibody Stripping Solution (Alpha Diagnostic Intl Inc, San Antonio, TX) for 10 min and blocked with 5% milk PBS-T for 20 min, then re-probed with anti-GAPDH antibody at a 1:800 dilution (Santa Cruz Biotech, Santa Cruz, CA) as an internal standard. .. Immunoblots were digitally scanned and densitometrically analyzed using Gel-Pro Analyzer (Media Cybernetics, Bethesda, MD).

Stripping:

Article Title: Uterine Dysfunction in Biglycan and Decorin Deficient Mice Leads to Dystocia during Parturition
Article Snippet: .. The membrane was stripped using Restore Western Blot stripping buffer (Thermo Scientific, Waltham, MA) for 10 min and blocked with 5% milk PBS-T for 20 min, then re-probed with anti-GAPDH antibody at a 1∶800 dilution (Santa Cruz Biotech, Santa Cruz, CA) as an internal standard. .. Immunoblots were digitally scanned and densitometrically analyzed using Gel-Pro Analyzer (Media Cybernetics, Bethesda, MD).

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  • 91
    Santa Cruz Biotechnology milk pbs t
    Milk Pbs T, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/milk pbs t/product/Santa Cruz Biotechnology
    Average 91 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    milk pbs t - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    91
    Santa Cruz Biotechnology milk pbs t buffer
    Milk Pbs T Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/milk pbs t buffer/product/Santa Cruz Biotechnology
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    milk pbs t buffer - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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