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TaKaRa mightymix ligation
Mightymix Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mightymix ligation/product/TaKaRa
Average 93 stars, based on 9 article reviews
Price from $9.99 to $1999.99
mightymix ligation - by Bioz Stars, 2019-12
93/100 stars

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Amplification:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry.

Ligation:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor. .. All plasmids were transformed into XL10-GOLD E . coli after ligation, harvested with a PureYield Miniprep kit (PROMEGA, Fitchburg, WI, USA), purified by phenol/chloroform extraction followed by ethanol precipitation and their sequence was confirmed by sequence analysis.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Ethanol Precipitation:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor.

Construct:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor. .. All plasmids were transformed into XL10-GOLD E . coli after ligation, harvested with a PureYield Miniprep kit (PROMEGA, Fitchburg, WI, USA), purified by phenol/chloroform extraction followed by ethanol precipitation and their sequence was confirmed by sequence analysis.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Purification:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor.

Polymerase Chain Reaction:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Expressing:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: To generate the hERα expression plasmid, full length D . magna EF1α-1 promoter [ ] and full length human esr1 [ ] were joined into a pRC21 backbone with full length EF1α-1 3’UTR via InFusion (TAKARA, Kusatsu, Shiga, Japan); the resulting construct was termed pRC21-hERa. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: To generate the hERα expression plasmid, full length D . magna EF1α-1 promoter [ ] and full length human esr1 [ ] were joined into a pRC21 backbone with full length EF1α-1 3’UTR via InFusion (TAKARA, Kusatsu, Shiga, Japan); the resulting construct was termed pRC21-hERa. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry.

Sequencing:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Transformation Assay:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor.

Plasmid Preparation:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: Paragraph title: Plasmid construction ... After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

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  • 93
    TaKaRa mightymix ligation
    Mightymix Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mightymix ligation/product/TaKaRa
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    mightymix ligation - by Bioz Stars, 2019-12
    93/100 stars
      Buy from Supplier

    80
    TaKaRa mightymix dna ligation kit
    Mightymix Dna Ligation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mightymix dna ligation kit/product/TaKaRa
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mightymix dna ligation kit - by Bioz Stars, 2019-12
    80/100 stars
      Buy from Supplier

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