mightymix ligation (TaKaRa)
93
Structured Review
TaKaRa
mightymix ligation
Mightymix Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mightymix ligation/product/TaKaRa
Average 93 stars, based on 9 article reviews
Price from $9.99 to $1999.99
Mightymix Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mightymix ligation/product/TaKaRa
Average 93 stars, based on 9 article reviews
Price from $9.99 to $1999.99
mightymix ligation - by Bioz Stars,
2019-12
93/100 stars
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Amplification:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Ligation:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Ethanol Precipitation:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Construct:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Purification:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Polymerase Chain Reaction:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: The dsred2 TALE site was amplified from genomic DNA with PCR primers introducing digestion sites for SalI and BssHII. .. After digestion, all three fragments were joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Expressing:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: To generate the hERα expression plasmid, full length D . magna EF1α-1 promoter [ ] and full length human esr1 [ ] were joined into a pRC21 backbone with full length EF1α-1 3’UTR via InFusion (TAKARA, Kusatsu, Shiga, Japan); the resulting construct was termed pRC21-hERa. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: To generate the hERα expression plasmid, full length D . magna EF1α-1 promoter [ ] and full length human esr1 [ ] were joined into a pRC21 backbone with full length EF1α-1 3’UTR via InFusion (TAKARA, Kusatsu, Shiga, Japan); the resulting construct was termed pRC21-hERa. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Sequencing:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. After digestion, all three fragments were joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Transformation Assay:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Plasmid Preparation:Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: Paragraph title: Plasmid construction ... After digestion, all three fragments were joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xERE sequence [ ] was amplified with primers introducing a restriction site for XmaI, it contains an EcoO109I site. .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna Article Snippet: A 4xEcRE promoter [ ] was amplified via polymerase chain reaction (PCR) with primers introducing a restriction site for MscI. .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via |