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TaKaRa mightymix ligation
Mightymix Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mightymix ligation/product/TaKaRa
Average 93 stars, based on 6 article reviews
Price from $9.99 to $1999.99
mightymix ligation - by Bioz Stars, 2020-07
93/100 stars

Related Products / Commonly Used Together

pcr fragment
ecoo109i
msci
xmai
mcherry plasmid

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Related Articles

Polymerase Chain Reaction:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Plasmid Preparation:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Construct:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor. .. All plasmids were transformed into XL10-GOLD E . coli after ligation, harvested with a PureYield Miniprep kit (PROMEGA, Fitchburg, WI, USA), purified by phenol/chloroform extraction followed by ethanol precipitation and their sequence was confirmed by sequence analysis.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Ligation:

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. After digestion, all three fragments were joined via MightyMix ligation (TAKARA); in the resulting construct the ERE reporter and the ER halves face opposite directions, it was termed pRC21-estrogensensor. .. All plasmids were transformed into XL10-GOLD E . coli after ligation, harvested with a PureYield Miniprep kit (PROMEGA, Fitchburg, WI, USA), purified by phenol/chloroform extraction followed by ethanol precipitation and their sequence was confirmed by sequence analysis.

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. This PCR fragment was also digested with EcoO109I and XmaI (NewEngland BioLabs) and joined into the backbone plasmid via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-ERE:mCherry. .. For genomic integration, pRC21-hERa as the backbone was digested with SalI and NdeI (NewEngland BioLabs). pRC21-ERE:mCherry was digested with BssHII and NdeI (NewEngland BioLabs).

Article Title: Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna
Article Snippet: .. Both the PCR fragment and the mCherry plasmid were digested with MscI and EcoO109I (NewEngland BioLabs, Ipswitch, MA, USA) and joined via MightyMix ligation (TAKARA); the resulting construct was termed pRC21-EcRE:mCherry. .. To generate the ERE reporter plasmid, the EcRE repeats were excised out of pRC21-EcRE:mCherry with EcoO109I and XmaI (NewEngland BioLabs).

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  • 93
    TaKaRa mightymix ligation
    Mightymix Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mightymix ligation/product/TaKaRa
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mightymix ligation - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    99
    TaKaRa mightymix dna ligation kit
    Mightymix Dna Ligation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mightymix dna ligation kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mightymix dna ligation kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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