Structured Review

Difco middlebrook 7h9 medium
Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
Middlebrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿"

Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

Journal:

doi: 10.1128/JB.00257-07

Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
Figure Legend Snippet: Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

Techniques Used:

2) Product Images from "Cdc48-like protein of actinobacteria (Cpa) is a novel proteasome interactor in mycobacteria and related organisms"

Article Title: Cdc48-like protein of actinobacteria (Cpa) is a novel proteasome interactor in mycobacteria and related organisms

Journal: eLife

doi: 10.7554/eLife.34055

M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in Middlebrook 7H9 medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.
Figure Legend Snippet: M. smegmatis Δ cpa shows normal growth behavior under standard culture conditions but displays a slight growth defect during carbon starvation. ( A ) Msm parent and knockout cells were cultured in Middlebrook 7H9 medium at 37°C and cell density was measured at 600 nm at the indicated time points. Both strains showed identical growth behavior indicating that Cpa is dispensable during standard cell culture conditions. ( B ) cpa -knockout cells show impaired growth in the same medium devoid of glycerol as a main carbon source. Both strains were cultured in a minimal medium in absence of glycerol at 37°C. Both growth curves are representative of three or more independent experiments with the mean values ± SD plotted.

Techniques Used: Knock-Out, Cell Culture

3) Product Images from "Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis"

Article Title: Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis

Journal: Molecular Microbiology

doi: 10.1111/j.1365-2958.2008.06365.x

Growth of M. tuberculosis mshA mutants in vitro . A. Growth in liquid media (Middlebrook 7H9 supplemented with 0.2% glycerol, OADC and 0.05% tyloxapol) of four mshA point mutants (left) or two Δ mshA mutants and their complemented strains (right). B. Growth on solid media of H37Rv Δ mshA (top right), H37Rv Δ mshA pMV361:: mshA (top left), CDC1551 Δ mshA (bottom right) and CDC1551 Δ mshA pMV361:: mshA (bottom left) on Middlebrook 7H10 plates supplemented with 0.2% glycerol and ADS, ADS + beef liver catalase, ADS + oleic acid or OADC. c = pMV361:: mshA .
Figure Legend Snippet: Growth of M. tuberculosis mshA mutants in vitro . A. Growth in liquid media (Middlebrook 7H9 supplemented with 0.2% glycerol, OADC and 0.05% tyloxapol) of four mshA point mutants (left) or two Δ mshA mutants and their complemented strains (right). B. Growth on solid media of H37Rv Δ mshA (top right), H37Rv Δ mshA pMV361:: mshA (top left), CDC1551 Δ mshA (bottom right) and CDC1551 Δ mshA pMV361:: mshA (bottom left) on Middlebrook 7H10 plates supplemented with 0.2% glycerol and ADS, ADS + beef liver catalase, ADS + oleic acid or OADC. c = pMV361:: mshA .

Techniques Used: In Vitro

4) Product Images from "N-acetyl-cysteine exhibits potent anti-mycobacterial activity in addition to its known anti-oxidative functions"

Article Title: N-acetyl-cysteine exhibits potent anti-mycobacterial activity in addition to its known anti-oxidative functions

Journal: BMC Microbiology

doi: 10.1186/s12866-016-0872-7

NAC restrains mycobacterial growth within THP-1 macrophages and exhibits a direct anti-mycobacterial effect on extracellular bacteria in vitro. a Human-THP-1 macrophages were infected with M. tuberculosis , M. avium or M. bovis strains at an MOI of 10 for 3 h. Extracellular bacteria were removed by washing. Cells were then cultivated for 5 days in the presence of NAC at 10 mM. CFU counts were assessed as described in Methods. b and c Mycobacteria strains were grown in Middlebrook 7H9 supplemented with OADC in 96-well plates. Metabolic activity measurements ( b ) and CFU counts ( c ) were performed as described in Methods. Significant differences were observed for the indicated experimental conditions compared to untreated cultures (* p
Figure Legend Snippet: NAC restrains mycobacterial growth within THP-1 macrophages and exhibits a direct anti-mycobacterial effect on extracellular bacteria in vitro. a Human-THP-1 macrophages were infected with M. tuberculosis , M. avium or M. bovis strains at an MOI of 10 for 3 h. Extracellular bacteria were removed by washing. Cells were then cultivated for 5 days in the presence of NAC at 10 mM. CFU counts were assessed as described in Methods. b and c Mycobacteria strains were grown in Middlebrook 7H9 supplemented with OADC in 96-well plates. Metabolic activity measurements ( b ) and CFU counts ( c ) were performed as described in Methods. Significant differences were observed for the indicated experimental conditions compared to untreated cultures (* p

Techniques Used: In Vitro, Infection, Activity Assay

NAC limits mycobacterial proliferation by acting as an anti-mycobacterial compound. Mycobacterium tuberculosis was grown in Middlebrook 7H9 supplemented with OADC as described in Methods. a Kinetic of mycobacteria growth during 7 days in the presence of NAC at indicated concentrations was verified using CFU counts. b Mycobacterial growth was evaluated at different pH as described in Methods. Fold increase of bacterial growth was calculated as the ratio of CFU number counted on days 0 and 5. Significant differences were observed for the indicated experimental conditions (*** p
Figure Legend Snippet: NAC limits mycobacterial proliferation by acting as an anti-mycobacterial compound. Mycobacterium tuberculosis was grown in Middlebrook 7H9 supplemented with OADC as described in Methods. a Kinetic of mycobacteria growth during 7 days in the presence of NAC at indicated concentrations was verified using CFU counts. b Mycobacterial growth was evaluated at different pH as described in Methods. Fold increase of bacterial growth was calculated as the ratio of CFU number counted on days 0 and 5. Significant differences were observed for the indicated experimental conditions (*** p

Techniques Used:

5) Product Images from "Mycobacterium tuberculosis protein kinase K confers survival advantage during early infection in mice and regulates growth in culture and during persistent infection: implications for immune modulation"

Article Title: Mycobacterium tuberculosis protein kinase K confers survival advantage during early infection in mice and regulates growth in culture and during persistent infection: implications for immune modulation

Journal: Microbiology

doi: 10.1099/mic.0.040675-0

TEM analysis of M. tuberculosis H37Rv (a) and the LIX11 mutant strain (b), showing differences in the staining pattern of the cell wall. Wild-type H37Rv and the LIX11 mutant were cultivated in Middlebrook 7H9-Tw-ADS medium to exponential phase (OD 600 0.3–0.4) and processed for electron microscopy in two separate experiments. One representative picture is shown. Scale bars, 0.2 μm. Arrows indicate dense granular staining present in the cell wall of the wild-type, but absent in LIX11.
Figure Legend Snippet: TEM analysis of M. tuberculosis H37Rv (a) and the LIX11 mutant strain (b), showing differences in the staining pattern of the cell wall. Wild-type H37Rv and the LIX11 mutant were cultivated in Middlebrook 7H9-Tw-ADS medium to exponential phase (OD 600 0.3–0.4) and processed for electron microscopy in two separate experiments. One representative picture is shown. Scale bars, 0.2 μm. Arrows indicate dense granular staining present in the cell wall of the wild-type, but absent in LIX11.

Techniques Used: Transmission Electron Microscopy, Mutagenesis, Staining, Electron Microscopy

6) Product Images from "A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival"

Article Title: A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival

Journal: PLoS ONE

doi: 10.1371/journal.pone.0021941

Knock-down of gre in mycobacteria is deleterious to the cell growth. ( A ) The pMV261-vector control, Mtb Gre anti-sense (pMV greAS ) and Mtb Gre over-expressing (pMV greOE ) M. smegmatis and M. tuberculosis H37Ra cells were grown for 18 hrs or 7 days respectively in liquid cultures at 37°C under shaking condition, serially diluted (10 −7 for vector control and over-expression and 10 −5 for anti-sense) and plated to determine the cell viability. ( B ) Growth curve of M. smegmatis cells with vector control, Mtb Gre anti-sense and Mtb Gre over-expression constructs. Cultures were diluted in Middlebrook 7H9 broth to give an initial OD 600 of 0.02 to 0.04 and incubated for 36 hours. The growth curves were plotted by measuring cell viability by dilution plating at different time points. ( C ) Western blot of the M. smegmatis cell lysates from vector control (lane 1) and anti-sense Mtb Gre construct (lane 2) using a polyclonal antibody against Mtb Gre. Purified Mtb Gre has been used as a positive control (lane 3).
Figure Legend Snippet: Knock-down of gre in mycobacteria is deleterious to the cell growth. ( A ) The pMV261-vector control, Mtb Gre anti-sense (pMV greAS ) and Mtb Gre over-expressing (pMV greOE ) M. smegmatis and M. tuberculosis H37Ra cells were grown for 18 hrs or 7 days respectively in liquid cultures at 37°C under shaking condition, serially diluted (10 −7 for vector control and over-expression and 10 −5 for anti-sense) and plated to determine the cell viability. ( B ) Growth curve of M. smegmatis cells with vector control, Mtb Gre anti-sense and Mtb Gre over-expression constructs. Cultures were diluted in Middlebrook 7H9 broth to give an initial OD 600 of 0.02 to 0.04 and incubated for 36 hours. The growth curves were plotted by measuring cell viability by dilution plating at different time points. ( C ) Western blot of the M. smegmatis cell lysates from vector control (lane 1) and anti-sense Mtb Gre construct (lane 2) using a polyclonal antibody against Mtb Gre. Purified Mtb Gre has been used as a positive control (lane 3).

Techniques Used: Plasmid Preparation, Expressing, Over Expression, Construct, Incubation, Western Blot, Purification, Positive Control

Related Articles

Centrifugation:

Article Title: A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival
Article Snippet: Bacterial strains, plasmids and the growth conditions M. smegmatis mc2 155 and M. smegmatis SM07sigA , were cultured in Middlebrook 7H9 medium (Difco) containing 0.05% Tween-80 (Sigma) and 0.4% glucose (Sigma) under shaking conditions at 37°C. .. To check the expression pattern of Gre at different growth phases in M. smegmatis and M. tuberculosis , cells were grown for 12, 18, 24, 30, 36 and 48 hrs (for M. smegmatis ) or 3, 5, 7, 12 and 20 days (for M. tuberculosis ), pelleted down by centrifugation, lysed by sonication and cell extracts were prepared.

Mutagenesis:

Article Title: Mycobacterium tuberculosis protein kinase K confers survival advantage during early infection in mice and regulates growth in culture and during persistent infection: implications for immune modulation
Article Snippet: Mycobacterium smegmatis mc2 155 was used for the isolation and propagation of recombinant mycobacteriophage for mutagenesis of pknK . .. M. tuberculosis cultures were cultivated in Middlebrook 7H9 medium (Difco) supplemented with 0.05 % Tween 80 (Tw) and ADS [0.5 % albumin/0.2 % dextrose (glucose)/0.085 % saline] (henceforth referred to as 7H9-Tw-ADS), or on Middlebrook 7H10 agar plates at 37 °C.

Isolation:

Article Title: Mycobacterium tuberculosis protein kinase K confers survival advantage during early infection in mice and regulates growth in culture and during persistent infection: implications for immune modulation
Article Snippet: Mycobacterium smegmatis mc2 155 was used for the isolation and propagation of recombinant mycobacteriophage for mutagenesis of pknK . .. M. tuberculosis cultures were cultivated in Middlebrook 7H9 medium (Difco) supplemented with 0.05 % Tween 80 (Tw) and ADS [0.5 % albumin/0.2 % dextrose (glucose)/0.085 % saline] (henceforth referred to as 7H9-Tw-ADS), or on Middlebrook 7H10 agar plates at 37 °C.

Cell Culture:

Article Title: A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival
Article Snippet: .. Bacterial strains, plasmids and the growth conditions M. smegmatis mc2 155 and M. smegmatis SM07sigA , were cultured in Middlebrook 7H9 medium (Difco) containing 0.05% Tween-80 (Sigma) and 0.4% glucose (Sigma) under shaking conditions at 37°C. .. M. tuberculosis H37Ra cells were cultured in Middlebrook 7H9 medium supplemented with ADC consisting of 0.2% glycerol (Sigma) and 0.05% Tween-80 at 37°C.

Article Title: Cdc48-like protein of actinobacteria (Cpa) is a novel proteasome interactor in mycobacteria and related organisms
Article Snippet: Mycobacterium smegmatis mc(2) 155 ( Msm ) was grown in liquid Middlebrook 7H9 medium (Difco) supplemented with 0.025% (w/v) Tween 80 and 0.2% (w/v) glycerol, unless otherwise stated. .. For carbon starvation, the cells were first cultured small-scale in minimal medium (adapted from ( ) containing: 40 mM K2 HPO4 , 22 mM KH2 PO4 , 15 mM (NH4 )2 SO4 , 1.7 mM sodium citrate, 0.4 mM MgSO4 , 0.05% (w/v) Tween 80 and 0.4% (w/v) glycerol.

other:

Article Title: N-acetyl-cysteine exhibits potent anti-mycobacterial activity in addition to its known anti-oxidative functions
Article Snippet: Mycobacteria from a single colony-forming unit (CFU) were suspended in Middlebrook 7H9 medium (Difco, BD Biosciences, USA) that was supplemented with 10 % albumin-dextrose-catalase (ADC; Difco) and 0.05 % Tween 80 (Sigma-Aldrich, USA), cultivated and then frozen at −80 °C in aliquots of 108 bacilli/mL.

Article Title: Regulation Mechanism of the ald Gene Encoding Alanine Dehydrogenase in Mycobacterium smegmatis and Mycobacterium tuberculosis by the Lrp/AsnC Family Regulator AldR
Article Snippet: M. smegmatis strains were grown in Middlebrook 7H9 medium (Difco, Sparks, MD) supplemented with 0.2% (wt/vol) glucose as a carbon source and 0.02% (vol/vol) Tween 80 as an anticlumping agent at 37°C.

Article Title: Treatment of Mycobacterium tuberculosis-Infected Macrophages with Poly(Lactic-Co-Glycolic Acid) Microparticles Drives NFκB and Autophagy Dependent Bacillary Killing
Article Snippet: Stocks were propagated in Middlebrook 7H9 medium (Difco/Becton Dickinson, Sparks, MD) made up in low-endotoxin water (Sigma, St. Louis, MO) supplemented with albumin-dextrose-catalase supplement (Becton Dickinson) and 0.05% Tween 80 (Difco).

Article Title: Biological Evaluation of Potent Triclosan-Derived Inhibitors of the Enoyl-Acyl Carrier Protein Reductase InhA in Drug-sensitive and Drug-resistant Strains of Mycobacterium tuberculosis
Article Snippet: The strains were grown in Middlebrook 7H9 medium (Difco) supplemented with 10% (v/v) OADC enrichment (Difco), 0.2% (v/v) glycerol, and 0.05% (v/v) tyloxapol.

Article Title: Pantothenate and Pantetheine Antagonize the Antitubercular Activity of Pyrazinamide
Article Snippet: The strains were grown in Middlebrook 7H9 medium (Difco) supplemented with 10% (vol/vol) oleic acid-albumin-dextrose-catalase (OADC) (Difco), 0.2% (vol/vol) glycerol, and 0.05% (vol/vol) tyloxapol. d , l -Pantothenate (50 μg ml−1 ) or d -pantethine (7.2 μg ml−1 ) was added for growth of M. tuberculosis strain mc2 7000.

Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿
Article Snippet: M. smegmatis mc2 155 was grown in liquid cultures using Middlebrook 7H9 medium (Difco) supplemented with 0.2% glycerol and 0.05% Tween 80 or minimal Hartmans-de Bont (HB) medium ( ) at 37°C.

Article Title: A 38-Kilobase Pathogenicity Island Specific for Mycobacterium avium subsp. paratuberculosis Encodes Cell Surface Proteins Expressed in the Host
Article Snippet: Mycobacteria were grown on Middlebrook 7H10 agar or in Middlebrook 7H9 medium (both from Difco Laboratories, Detroit, Mich.) supplemented with oleic acid-albumin-dextrose-catalase enrichment (Difco), Tween 80 (0.05%), and kanamycin (40 μg ml−1 ) for Mycobacterium smegmatis transformants; for M. avium subsp. paratuberculosis culture, mycobactin (2 μg ml−1 ) (Synbiotics, Lyon, France) was added.

Article Title: Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis
Article Snippet: The strains were grown in Middlebrook 7H9 medium (Difco) supplemented with 10% (v/v) OADC enrichment (Difco), 0.2% (v/v) glycerol and 0.05% (v/v) tyloxapol.

Article Title: Reactivation of latent tuberculosis by an inhibitor of inducible nitric oxide synthase in an aerosol murine model
Article Snippet: M. tuberculosis H37Rv (obtained from G. Kaplan, Rockefeller University, New York, NY) was grown in Middlebrook 7H9 medium (Difco Laboratories, Detroit, MI), supplemented with 10% oleic acid albumin dextrose catalase (OADC) (State Vaccine Institute, Pinelands, South Africa) and 1% glycerol (Merck, München, Germany), in 5% CO2 at 37°, and frozen in aliquots.

Expressing:

Article Title: A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival
Article Snippet: Bacterial strains, plasmids and the growth conditions M. smegmatis mc2 155 and M. smegmatis SM07sigA , were cultured in Middlebrook 7H9 medium (Difco) containing 0.05% Tween-80 (Sigma) and 0.4% glucose (Sigma) under shaking conditions at 37°C. .. To check the expression pattern of Gre at different growth phases in M. smegmatis and M. tuberculosis , cells were grown for 12, 18, 24, 30, 36 and 48 hrs (for M. smegmatis ) or 3, 5, 7, 12 and 20 days (for M. tuberculosis ), pelleted down by centrifugation, lysed by sonication and cell extracts were prepared.

Article Title: Mycobacterium tuberculosis protein kinase K confers survival advantage during early infection in mice and regulates growth in culture and during persistent infection: implications for immune modulation
Article Snippet: M. tuberculosis H37Rv and H37Ra strains were analysed for differences in STPK gene expression. .. M. tuberculosis cultures were cultivated in Middlebrook 7H9 medium (Difco) supplemented with 0.05 % Tween 80 (Tw) and ADS [0.5 % albumin/0.2 % dextrose (glucose)/0.085 % saline] (henceforth referred to as 7H9-Tw-ADS), or on Middlebrook 7H10 agar plates at 37 °C.

Sonication:

Article Title: A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival
Article Snippet: Bacterial strains, plasmids and the growth conditions M. smegmatis mc2 155 and M. smegmatis SM07sigA , were cultured in Middlebrook 7H9 medium (Difco) containing 0.05% Tween-80 (Sigma) and 0.4% glucose (Sigma) under shaking conditions at 37°C. .. To check the expression pattern of Gre at different growth phases in M. smegmatis and M. tuberculosis , cells were grown for 12, 18, 24, 30, 36 and 48 hrs (for M. smegmatis ) or 3, 5, 7, 12 and 20 days (for M. tuberculosis ), pelleted down by centrifugation, lysed by sonication and cell extracts were prepared.

Recombinant:

Article Title: Mycobacterium tuberculosis protein kinase K confers survival advantage during early infection in mice and regulates growth in culture and during persistent infection: implications for immune modulation
Article Snippet: Mycobacterium smegmatis mc2 155 was used for the isolation and propagation of recombinant mycobacteriophage for mutagenesis of pknK . .. M. tuberculosis cultures were cultivated in Middlebrook 7H9 medium (Difco) supplemented with 0.05 % Tween 80 (Tw) and ADS [0.5 % albumin/0.2 % dextrose (glucose)/0.085 % saline] (henceforth referred to as 7H9-Tw-ADS), or on Middlebrook 7H10 agar plates at 37 °C.

Plasmid Preparation:

Article Title: A Transcript Cleavage Factor of Mycobacterium tuberculosis Important for Its Survival
Article Snippet: Bacterial strains, plasmids and the growth conditions M. smegmatis mc2 155 and M. smegmatis SM07sigA , were cultured in Middlebrook 7H9 medium (Difco) containing 0.05% Tween-80 (Sigma) and 0.4% glucose (Sigma) under shaking conditions at 37°C. .. Knock-down of gre expression in M. smegmatis mc2 155 and M. tuberculosis H37Ra was carried out by generating the plasmid pMVgreAS (Mtbgre in anti-sense orientation) in pMV261 .

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  • 94
    Difco 7h9 liquid medium
    Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard <t>7H9</t> medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.
    7h9 Liquid Medium, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h9 liquid medium/product/Difco
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    7h9 liquid medium - by Bioz Stars, 2020-04
    94/100 stars
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    95
    Difco middlebrook 7h9 medium
    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in <t>Middlebrook</t> <t>7H9</t> medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation
    Middlebrook 7h9 Medium, supplied by Difco, used in various techniques. Bioz Stars score: 95/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h9 medium/product/Difco
    Average 95 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h9 medium - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    94
    Difco middlebrook 7h9
    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on <t>Middlebrook</t> 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook <t>7H9</t> liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .
    Middlebrook 7h9, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h9/product/Difco
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h9 - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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    Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard 7H9 medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.

    Journal: BMC Research Notes

    Article Title: Nitrogen starvation-induced transcriptome alterations and influence of transcription regulator mutants in Mycobacterium smegmatis

    doi: 10.1186/1756-0500-6-482

    Figure Lengend Snippet: Growth of M. smegmatis in various nitrogen sources. Wild-type SMR5 and glnR deletion strain MH1 were grown in the presence of the indicated substances (10 mM final concentration) as sole nitrogen source. Standard 7H9 medium was used as positive control; 7H9 lacking any nitrogen source as negative control (7H9-N). (A) Putative nitrogen sources deduced from microarray results, (B) putative nitrogen sources used by closely related species.

    Article Snippet: Mycobacterial strains were grown in Middlebrook 7H9 liquid medium (Difco Laboratories; per 900 ml approx.

    Techniques: Concentration Assay, Positive Control, Negative Control, Microarray

    Effect of DETA-NO on growth of cultured M. bovis BCG. Bacteria growing exponentially in 7H9 medium were transferred to evacuated (Vacutainer) tubes at mid-log phase, and at time zero either the cells were left untreated (open circles) or DETA-NO was added

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vitro Model of Mycobacterial Growth Arrest Using Nitric Oxide with Limited Air ▿

    doi: 10.1128/AAC.00442-08

    Figure Lengend Snippet: Effect of DETA-NO on growth of cultured M. bovis BCG. Bacteria growing exponentially in 7H9 medium were transferred to evacuated (Vacutainer) tubes at mid-log phase, and at time zero either the cells were left untreated (open circles) or DETA-NO was added

    Article Snippet: M. bovis BCG, substrain Pasteur, isolate KD1295 , was grown in Middlebrook 7H9 liquid medium (catalog no. 271310; Difco) or on 7H10 agar medium (catalog no. 262710; Difco) supplemented with 10% albumin dextrose complex, 0.2% glycerol, and 0.05% Tween 80 ( ).

    Techniques: Cell Culture

    Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Journal:

    Article Title: A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis ▿

    doi: 10.1128/JB.00257-07

    Figure Lengend Snippet: Kinetics and inducibility of fructose, glucose, and glycerol uptake by M. smegmatis . (A) M. smegmatis mc 2 155 was grown in Middlebrook 7H9 medium in the presence of 2% glycerol (open circles) or 2% fructose (closed circles). Accumulation

    Article Snippet: M. smegmatis mc2 155 was grown in liquid cultures using Middlebrook 7H9 medium (Difco) supplemented with 0.2% glycerol and 0.05% Tween 80 or minimal Hartmans-de Bont (HB) medium ( ) at 37°C.

    Techniques:

    Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Journal: Frontiers in Microbiology

    Article Title: Functional Studies of Five Toxin-Antitoxin Modules in Mycobacterium tuberculosis H37Rv

    doi: 10.3389/fmicb.2016.02071

    Figure Lengend Snippet: Toxic effect of the five toxins on the growth of M. smegmatis . (A) Transformation of pMV306AC-toxin plasmid into M. smegmatis mc 2 155. One microgram of DNA was electrophorated and cells were plated on Middlebrook 7H10 solid media. The plates were incubated for 3 days at 37°C. (B) Effect of toxin on the induction on colony formation of M. smegmatis mc 2 155. Each transformant from (A) was grown for 3 days at 37°C. The culture was diluted 100-fold into Middlebrook 7H9 liquid media and streaked on Middlebrook 7H10 solid media in the absence and presence of 0.2% acetamide. The numbers in the circle correspond to those in (A) .

    Article Snippet: M. smegmatis mc2 155 was grown in Middlebrook 7H9 (a liquid medium, Difco) containing 0.2% glycerol and 0.02% Tween 80 or Middlebrook 7H10 (a solid agar medium, Difco) containing 0.2% glycerol.

    Techniques: Transformation Assay, Plasmid Preparation, Incubation