Structured Review

Fisher Scientific microtubes
Microtubes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtubes/product/Fisher Scientific
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
microtubes - by Bioz Stars, 2020-09
92/100 stars

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Sonication:

Article Title: Quantitative analysis of cell-free DNA in the plasma of gastric cancer patients
Article Snippet: .. For the quantification of fragmented DNA, including cfDNA, 0.3 ml of intact genomic DNA (10 ng/µl) (Clontech Laboratories, Mountain View, CA, USA) was placed into 1.5-ml microtubes in ice water and sonicated with a model 100 Ultrasonic Dismembrator (Fisher Scientific, Pittsburgh, PA, USA) at various times (5, 10, 20, 30 and 50 sec) to generate different size distributions. .. The concentration of the fragmented DNA was then estimated using three methods: NanoDrop 1000 using a UV-Vis spectrophotometer (Thermo Scientific, Waltham, MA, USA), NanoDrop 3300 (Thermo Scientific) using the PicoGreen fluorophore and real-time qPCR by amplifying 81-bp Alu DNA sequences (Alu81-qPCR).

Chloramphenicol Acetyltransferase Assay:

Article Title: Quantifying Antibody Responses Induced by Antigen-Agnostic Immunotherapies
Article Snippet: .. ○ 1.5 mL microtubes ○ Gauze pads ○ Eye lubricant ○ Container filled with ice pellets • Cell Culture ○ Complete DMEM (Fisher Scientific, CatSH30022.01) or media specific to the tumor cell line of interest ○ 10% fetal bovine serum (VWR, PA, USA, Cat#97068-085) ○ Penicillin streptomycin cocktail (Fisher Scientific, Cat#SV30010) ○ 0.25% EDTA (Corning, NY, USA, reference number [Ref#]25-052-CI) ○ PBS (Fisher Scientific, Cat#SH30256.01) ○ Cell culture-treated flasks and plates, including 96-well flat-bottom plates ○ Fibronectic-collagen (FNC) coating mix (AthenaES, MD, USA, Cat#0407) • Sample Processing ○ HBSS (Fisher Scientific, Cat#SH3003103) ○ 4% Paraformaldehyde (Fisher Scientific, Cat#J19943-K2) ○ 0.2% Triton X-100 (Sigma-Aldrich, MA, USA, Cat#T8787) ○ BSA (Fisher Scientific, Cat#BP1600100) • Antibody ○ Goat anti-mouse IgG (H+L)-Alexa Fluor 488 (Fisher Scientific, Cat # A28175). ..

Cell Culture:

Article Title: Quantifying Antibody Responses Induced by Antigen-Agnostic Immunotherapies
Article Snippet: .. ○ 1.5 mL microtubes ○ Gauze pads ○ Eye lubricant ○ Container filled with ice pellets • Cell Culture ○ Complete DMEM (Fisher Scientific, Cat#SH30022.01) or media specific to the tumor cell line of interest ○ 10% fetal bovine serum (VWR, PA, USA, Cat#97068-085) ○ Penicillin streptomycin cocktail (Fisher Scientific, Cat#SV30010) ○ 0.25% EDTA (Corning, NY, USA, reference number [Ref#]25-052-CI) ○ PBS (Fisher Scientific, Cat#SH30256.01) ○ Cell culture-treated flasks and plates, including 96-well flat-bottom plates ○ Fibronectic-collagen (FNC) coating mix (AthenaES, MD, USA, Cat#0407) • Sample Processing ○ HBSS (Fisher Scientific, Cat#SH3003103) ○ 4% Paraformaldehyde (Fisher Scientific, Cat#J19943-K2) ○ 0.2% Triton X-100 (Sigma-Aldrich, MA, USA, Cat#T8787) ○ BSA (Fisher Scientific, Cat#BP1600100) • Antibody ○ Goat anti-mouse IgG (H+L)-Alexa Fluor 488 (Fisher Scientific, Cat # A28175). ..

Blocking Assay:

Article Title: Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues
Article Snippet: .. 4°C and −20°C storage units Centrifuge for 1.5- and 2-ml tubes Dry block heater for microtubes at 80°C Falcon conical centrifuge tubes (15 ml and 50 ml) (Fisher Scientific, cat. nos. .. 14-959-49B and 14-432-22) Flexible plastic I.V. catheter for reagent aspiration (Terumo, part no. SR*FF2419) CAUTION The catheter comes with a plastic outer sheath and a sharp needle in the middle.

Size-exclusion Chromatography:

Article Title: Quantitative analysis of cell-free DNA in the plasma of gastric cancer patients
Article Snippet: .. For the quantification of fragmented DNA, including cfDNA, 0.3 ml of intact genomic DNA (10 ng/µl) (Clontech Laboratories, Mountain View, CA, USA) was placed into 1.5-ml microtubes in ice water and sonicated with a model 100 Ultrasonic Dismembrator (Fisher Scientific, Pittsburgh, PA, USA) at various times (5, 10, 20, 30 and 50 sec) to generate different size distributions. .. The concentration of the fragmented DNA was then estimated using three methods: NanoDrop 1000 using a UV-Vis spectrophotometer (Thermo Scientific, Waltham, MA, USA), NanoDrop 3300 (Thermo Scientific) using the PicoGreen fluorophore and real-time qPCR by amplifying 81-bp Alu DNA sequences (Alu81-qPCR).

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  • 88
    Fisher Scientific polypropylene microtube
    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
    Polypropylene Microtube, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene microtube/product/Fisher Scientific
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polypropylene microtube - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    88
    Fisher Scientific rnase free microtubes
    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
    Rnase Free Microtubes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free microtubes/product/Fisher Scientific
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase free microtubes - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    88
    Fisher Scientific recessed polyimide microtube
    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
    Recessed Polyimide Microtube, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recessed polyimide microtube/product/Fisher Scientific
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recessed polyimide microtube - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    92
    Fisher Scientific microtubes
    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a <t>microtube</t> precoated with lithium
    Microtubes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtubes/product/Fisher Scientific
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microtubes - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium

    Journal: Journal of the American Association for Laboratory Animal Science : JAALAS

    Article Title: Miniaturized Blood Sampling Techniques to Benefit Reduction in Mice and Refinement in Nonhuman Primates: Applications to Bioanalysis in Toxicity Studies with Antibody–Drug Conjugates

    doi:

    Figure Lengend Snippet: Procedure for blood sampling in lightly restrained cynomolgus monkeys. (A) The lateral side of the heel is visualized. (B) After local disinfection and puncture with a 21-gauge needle, blood (50 µL) is collected in a microtube precoated with lithium

    Article Snippet: An exact volume of 10 µL plasma was withdrawn and transferred (as described earlier in part 1) into a 1-mL polypropylene microtube (NUNC, Fisher Scientific) prefilled with 990 µL of a buffer solution (0.05% Tween 20 and 0.5% BSA in PBS).

    Techniques: Sampling