microtube afa fiber  (Covaris)


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    Structured Review

    Covaris microtube afa fiber
    Microtube Afa Fiber, supplied by Covaris, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtube afa fiber/product/Covaris
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microtube afa fiber - by Bioz Stars, 2020-02
    83/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: Paragraph title: Modern DNA extraction and fragmentation ... For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst.

    Sample Prep:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: Aliquots of 500 ng of E. coli and E. h. onager extracts were subjected to three parallel fragmentation procedures, using either the Bioruptor NGS (Diagenode), the Covaris E210 sonicator (Covaris) or a Nebulizer Kit (part of the Paired-End DNA Sample Preparation Kit, Illumina). .. For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst.

    Sonication:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: .. For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst. .. For the nebulization procedure, DNA extracts were diluted up to 50 µl in TE buffer, and added to 700 µl nebulization buffer (53% Glycerol, 37 mM Tris-HCL, 5.5 mM EDTA) in Illumina supplied nebulizers, and subjected to 2.4 bar compressed nitrogen for 8 min. Nebulized products were purified on a Qiaquick column following manufacturer’s instructions and eluted in 50 µl EB.

    Next-Generation Sequencing:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: Aliquots of 500 ng of E. coli and E. h. onager extracts were subjected to three parallel fragmentation procedures, using either the Bioruptor NGS (Diagenode), the Covaris E210 sonicator (Covaris) or a Nebulizer Kit (part of the Paired-End DNA Sample Preparation Kit, Illumina). .. For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst.

    Purification:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst. .. For the nebulization procedure, DNA extracts were diluted up to 50 µl in TE buffer, and added to 700 µl nebulization buffer (53% Glycerol, 37 mM Tris-HCL, 5.5 mM EDTA) in Illumina supplied nebulizers, and subjected to 2.4 bar compressed nitrogen for 8 min. Nebulized products were purified on a Qiaquick column following manufacturer’s instructions and eluted in 50 µl EB.

    Concentration Assay:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: We measured the concentration of all fresh DNA extracts using a Qubit dsDNA HS assay (Invitrogen). .. For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst.

    Incubation:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: 69506), following manufacturer’s instructions and 1 h incubation in the lysis buffer. .. For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst.

    Gel Extraction:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst. .. All Qiagen column purification steps in this study were done using a final elution step at 37°C for 15 min. Fragmented DNA samples were run on a 2% agarose gel, along with a 50 bp ladder (GeneRuler, Thermoscientific), size selected between 125–175 bp and purified using the QIAquick Gel Extraction Kit (QIAGEN, reference nb.

    Agarose Gel Electrophoresis:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst. .. All Qiagen column purification steps in this study were done using a final elution step at 37°C for 15 min. Fragmented DNA samples were run on a 2% agarose gel, along with a 50 bp ladder (GeneRuler, Thermoscientific), size selected between 125–175 bp and purified using the QIAquick Gel Extraction Kit (QIAGEN, reference nb.

    Lysis:

    Article Title: Ligation Bias in Illumina Next-Generation DNA Libraries: Implications for Sequencing Ancient Genomes
    Article Snippet: 69506), following manufacturer’s instructions and 1 h incubation in the lysis buffer. .. For Covaris fragmentation, DNA extracts were diluted up to 130 µl in TE buffer in microTUBE AFA Fiber with Crimp-Cap (Covaris), and sonicated for 480 seconds using a duty cycle of 10%, an intensity of 5, and 200 cycles per burst.

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    The Covaris microTUBE 50 is engineered to optimize DNA Shearing in a 50 µl sample volume with Focused ultrasonicators utilizing Adaptive Focused Acoustics AFA technology
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    Covaris covaris s220
    Covaris S220, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris s220/product/Covaris
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    covaris s220 - by Bioz Stars, 2020-02
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    Covaris microtube afa fiber screw cap
    Microtube Afa Fiber Screw Cap, supplied by Covaris, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtube afa fiber screw cap/product/Covaris
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    microtube afa fiber screw cap - by Bioz Stars, 2020-02
    93/100 stars
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    83
    Covaris microtube afa fiber
    Microtube Afa Fiber, supplied by Covaris, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtube afa fiber/product/Covaris
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microtube afa fiber - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

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