Structured Review

Yamato Kohki Industrial Co Ltd microtome
Microtome, supplied by Yamato Kohki Industrial Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Yamato Kohki Industrial Co Ltd
Average 94 stars, based on 12 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
94/100 stars

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Micro-CT:

Article Title: Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model
Article Snippet: Histology Following micro-CT imaging, femurs were submerged in 20% EDTA for four weeks for demineralization. .. The resulting tissue was embedded in paraffin using an automatic tissue processor (Tissue-Tek VIP 6; Sakura Fine Tek, Tokyo, Japan) and sectioned through the femur's long axis at 3 μ m in the sagittal plane using a microtome (REM-710; Yamato Kohki industrial Co. Ltd., Saitama, Japan).

Immunostaining:

Article Title: Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist
Article Snippet: .. For immunostaining, serial section (30 μm thick) were cut using a microtome (REM-700; Yamato Kohki Industrial Co., Ltd., Saitama, Japan). ..

Modification:

Article Title: A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains
Article Snippet: The embedded sections were cut into 10 μm-slices using a microtome (Yamato Kohki Industrial Co., Ltd., PR-50, Saitama, Japan). .. For pre-hybridization, the solution was placed onto each slide containing the sections and it was incubated at 55 °C for 1 h. The solution was then replaced with the same solution containing DIG-labeled RNA probes at the concentration of 5 ng•ml−1 , and the slides were incubated at 55 °C for 16 h. The washing, detection and microscopic observation steps after hybridization were performed as described previously with slight modification (Sakurai et al. ; Ohashi et al. ).

Incubation:

Article Title: A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains
Article Snippet: The embedded sections were cut into 10 μm-slices using a microtome (Yamato Kohki Industrial Co., Ltd., PR-50, Saitama, Japan). .. For pre-hybridization, the solution was placed onto each slide containing the sections and it was incubated at 55 °C for 1 h. The solution was then replaced with the same solution containing DIG-labeled RNA probes at the concentration of 5 ng•ml−1 , and the slides were incubated at 55 °C for 16 h. The washing, detection and microscopic observation steps after hybridization were performed as described previously with slight modification (Sakurai et al. ; Ohashi et al. ).

Article Title: Use of [18F]FDOPA-PET for in vivo evaluation of dopaminergic dysfunction in unilaterally 6-OHDA-lesioned rats
Article Snippet: Sections of 40 μm were cut using a sliding microtome (Retoratome REM-700, Yamato Kohki Industrial Co., Ltd., Saitama, Japan) with a refrigeration unit (Electro Freeze MC-802A, Yamato Kohki Industrial Co., Ltd.). .. After three 5-min washes in TPBS, sections were preincubated with blocking solution (Block Ace™; DS Pharma Biomedical Co., Ltd., Osaka, Japan) at room temperature for 60 min and incubated overnight at 4°C with a 1:30,000 dilution of mouse anti-TH monoclonal antibody (ImmunoStar, Inc., Hudson, WI, USA) in blocking solution.

Article Title: Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist
Article Snippet: For immunostaining, serial section (30 μm thick) were cut using a microtome (REM-700; Yamato Kohki Industrial Co., Ltd., Saitama, Japan). .. Floating sections were incubated with 1% hydrogen peroxide for 60 min, followed by a rabbit polyclonal anti-Fos protein antiserum (#sc-52, Santa Cruz Biotechnology, Santa Cruz, CA, USA) diluted at 1:500 in 0.1 M PBS containing 0.3% Triton X-100 at 4 °C for 4 days.

Activity Assay:

Article Title: Use of [18F]FDOPA-PET for in vivo evaluation of dopaminergic dysfunction in unilaterally 6-OHDA-lesioned rats
Article Snippet: Sections of 40 μm were cut using a sliding microtome (Retoratome REM-700, Yamato Kohki Industrial Co., Ltd., Saitama, Japan) with a refrigeration unit (Electro Freeze MC-802A, Yamato Kohki Industrial Co., Ltd.). .. Endogenous peroxidase activity was quenched for 30 min in PBS containing 0.3% H2 O2 .

Expressing:

Article Title: Activation of endogenous arginine vasopressin neurons inhibit food intake: by using a novel transgenic rat line with DREADDs system
Article Snippet: Paragraph title: Expression of hM3Dq-mCherry in AVP neurons ... Fixed tissue was cut at 40 μm by using a microtome (REM-700; Yamato Kohki Industrial Co. Ltd, Saitama, Japan).

Transplantation Assay:

Article Title: Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1
Article Snippet: Paragraph title: Transplantation into the non-human primate PD models ... The brains were cut with a microtome (REM-710; YAMATO KOHKI Industrial Co., Ltd) at 40 μm thickness and mounted.

Blocking Assay:

Article Title: Use of [18F]FDOPA-PET for in vivo evaluation of dopaminergic dysfunction in unilaterally 6-OHDA-lesioned rats
Article Snippet: Sections of 40 μm were cut using a sliding microtome (Retoratome REM-700, Yamato Kohki Industrial Co., Ltd., Saitama, Japan) with a refrigeration unit (Electro Freeze MC-802A, Yamato Kohki Industrial Co., Ltd.). .. After three 5-min washes in TPBS, sections were preincubated with blocking solution (Block Ace™; DS Pharma Biomedical Co., Ltd., Osaka, Japan) at room temperature for 60 min and incubated overnight at 4°C with a 1:30,000 dilution of mouse anti-TH monoclonal antibody (ImmunoStar, Inc., Hudson, WI, USA) in blocking solution.

Hybridization:

Article Title: A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains
Article Snippet: The embedded sections were cut into 10 μm-slices using a microtome (Yamato Kohki Industrial Co., Ltd., PR-50, Saitama, Japan). .. Pre-hybridization and hybridization solution contained 50% formamide, 300 mM NaCl, 10 mM Tris–HCl (pH 7.4), 1 mM EDTA, 100 μg•ml−1 herring sperm DNA, 100 μg•ml−1 yeast tRNA, and 0.25% (w/v) SDS.

Immunohistochemistry:

Article Title: Use of [18F]FDOPA-PET for in vivo evaluation of dopaminergic dysfunction in unilaterally 6-OHDA-lesioned rats
Article Snippet: Paragraph title: Tyrosine hydroxylase immunohistochemistry ... Sections of 40 μm were cut using a sliding microtome (Retoratome REM-700, Yamato Kohki Industrial Co., Ltd., Saitama, Japan) with a refrigeration unit (Electro Freeze MC-802A, Yamato Kohki Industrial Co., Ltd.).

Article Title: YAP-dependent necrosis occurs in early stages of Alzheimer’s disease and regulates mouse model pathology
Article Snippet: Paragraph title: Immunohistochemistry ... Sagittal or coronal sections (5 μm thickness) were obtained using a microtome (REM-710, Yamato Kohki Industrial Co., Ltd., Saitama, Japan).

Article Title: Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist
Article Snippet: Paragraph title: Fos immunohistochemistry and double immunohistochemical staining for Fos and orexin-A ... For immunostaining, serial section (30 μm thick) were cut using a microtome (REM-700; Yamato Kohki Industrial Co., Ltd., Saitama, Japan).

Cell Culture:

Article Title: Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1
Article Snippet: The cultured spheres in Neurobasal medium supplemented with 1% P/S, 0.1 mM 2-ME, 200 nM AA, 2 mM L -Gln, 400 mM dbcAMP, 10 ng ml−1 GDNF, 20 ng ml−1 BDNF and 1 × B-27 supplement, and 30 μM Y-27632 were injected stereotactically through a 22G needle into the right striatum along four tracts/side (∼1 × 106 cells in 4 μl per 4 injection sites per tract). .. The brains were cut with a microtome (REM-710; YAMATO KOHKI Industrial Co., Ltd) at 40 μm thickness and mounted.

Light Microscopy:

Article Title: Effect of ionic liquid treatment on the ultrastructural and topochemical features of compression wood in Japanese cedar (Cryptomeria japonica)
Article Snippet: .. Light microscopy analysis The extracted samples were sectioned to 15-μm-thick with a sliding microtome (TU-213, Yamato Kohki Industrial Co., Ltd., Saitama, Japan) and mounted in a 20-μm-deep hemocytometer (Sunlead Glass Corp., Saitama, Japan). ..

Article Title: Exosomes from conditioned media of bone marrow-derived mesenchymal stem cells promote bone regeneration by enhancing angiogenesis
Article Snippet: After 4 weeks of decalcification, samples were embedded in paraffin and sectioned at 4 μm thickness in the coronal plane using a microtome (REM-710, YAMATO KOHKI Industrial Co., Ltd., Saitama, Japan). .. Sections were dewaxed, rehydrated, stained with hematoxylin-eosin, and analyzed using a light microscope (FX630, OLYMPUS Co., Tokyo, Japan) and FLVFS-LS software (OLYMPUS Co.).

Imaging:

Article Title: Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model
Article Snippet: Histology Following micro-CT imaging, femurs were submerged in 20% EDTA for four weeks for demineralization. .. The resulting tissue was embedded in paraffin using an automatic tissue processor (Tissue-Tek VIP 6; Sakura Fine Tek, Tokyo, Japan) and sectioned through the femur's long axis at 3 μ m in the sagittal plane using a microtome (REM-710; Yamato Kohki industrial Co. Ltd., Saitama, Japan).

Injection:

Article Title: Activation of endogenous arginine vasopressin neurons inhibit food intake: by using a novel transgenic rat line with DREADDs system
Article Snippet: Expression of hM3Dq-mCherry in AVP neurons The rats were deeply anesthetized with intraperitoneal injection of cocktail of three different anaesthetic agents (in combination with 0.3 mg/kg of medetomidine, 4.0 mg/kg of midazolam, and 5.0 mg/kg of butorphanol). .. Fixed tissue was cut at 40 μm by using a microtome (REM-700; Yamato Kohki Industrial Co. Ltd, Saitama, Japan).

Article Title: Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1
Article Snippet: After surgery, the monkeys received antibiotics for 3 days and intramuscular injection of the immunosuppressant FK506 (0.05 mg kg−1 , Astellas, Tokyo, Japan) until the day of sacrifice. .. The brains were cut with a microtome (REM-710; YAMATO KOHKI Industrial Co., Ltd) at 40 μm thickness and mounted.

Article Title: Effect of oestrogen-dependent vasopressin on HPA axis in the median eminence of female rats
Article Snippet: Extraction of hypothalamus and pituitary gland The male and female rats were anaesthetised with intraperitoneal injection of a cocktail of three different anaesthetic agents. .. Fixed tissue was cut to 40-μm thickness using a microtome (REM-700; Yamato Kohki Industrial Co. Ltd, Saitama, Japan) to generate coronal sections.

Article Title: Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist
Article Snippet: Ninety minutes after the icv injection of ghrelin or saline, the rats were deeply anesthetized by ip injection of sodium pentobarbital (50 mg/kg). .. For immunostaining, serial section (30 μm thick) were cut using a microtome (REM-700; Yamato Kohki Industrial Co., Ltd., Saitama, Japan).

Immunofluorescence:

Article Title: Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1
Article Snippet: The brains were cut with a microtome (REM-710; YAMATO KOHKI Industrial Co., Ltd) at 40 μm thickness and mounted. .. Every 36 sections containing the graft region were chosen for the immunofluorescence study.

Article Title: Megalin Blockade with Cilastatin Suppresses Drug-Induced Nephrotoxicity
Article Snippet: Paragraph title: Preparation of Kidney Sections for PAS and Immunofluorescence Staining ... From each tissue sample, 2- μ m-thick sections were cut using a microtome (REM-710; Yamato Kohki Industrial Co., Ltd.) and stained with PAS.

Antibody Labeling:

Article Title: YAP-dependent necrosis occurs in early stages of Alzheimer’s disease and regulates mouse model pathology
Article Snippet: Sagittal or coronal sections (5 μm thickness) were obtained using a microtome (REM-710, Yamato Kohki Industrial Co., Ltd., Saitama, Japan). .. For multi-labeling, antibodies were labeled by Zenon Secondary Detection-Based Antibody Labeling Kits as follows: anti-calnexin, anti-RIP3, anti-pSer166-RIP1 and anti-pSer232-RIP3 (Zenon™ Alexa Fluor™ 555 Rabbit IgG Labeling Kit, Z-25305, Thermo Fisher Scientific, Waltham, MA, USA); anti-MAP2 (Zenon™ Alexa Fluor™ 647 Rabbit IgG Labeling Kit, Z-25308 and Zenon™ Alexa Fluor™ 488 Rabbit IgG Labeling Kit, Z-25302, Thermo Fisher Scientific, Waltham, MA, USA); anti-pSer345-MLKL (Zenon™ Alexa Fluor™ 647 Rabbit IgG Labeling Kit, Z25308 Thermo Fisher Scientific, Waltham, MA, USA); anti-amyloid β (Zenon™ Alexa Fluor™ 488 Mouse IgG1 Labeling Kit, Z-25002, Thermo Fisher Scientific, Waltham, MA, USA).

Avidin-Biotin Assay:

Article Title: Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist
Article Snippet: For immunostaining, serial section (30 μm thick) were cut using a microtome (REM-700; Yamato Kohki Industrial Co., Ltd., Saitama, Japan). .. After washing in 0.3% Triton X-100/PBS for 20 min, the sections were further incubated for 120 min with a biotinylated secondary antibody solution (1:250) and finally with an avidin–biotin peroxidase complex (Vector Laboratories Inc., Burlingame, CA, USA) for 120 min.

Microscopy:

Article Title: Activation of endogenous arginine vasopressin neurons inhibit food intake: by using a novel transgenic rat line with DREADDs system
Article Snippet: Fixed tissue was cut at 40 μm by using a microtome (REM-700; Yamato Kohki Industrial Co. Ltd, Saitama, Japan). .. The sections, containing the SCN, SON, and PVN, were examined by fluorescent microscopy (ECLIPSEE 600; Nikon Corp., Tokyo, Japan) with an RFP filter (Nikon Corp.) to visualise hM3Dq-mCherry expression.

Mouse Assay:

Article Title: A new dry eye mouse model produced by exorbital and intraorbital lacrimal gland excision
Article Snippet: Paragraph title: C57BL/6 WT mice samples for hematoxylin-eosin staining ... Approximately 3-µm thickness serial sections were cut using a sliding microtome (ROM-380; Yamato Kohki Industrial Co., Ltd., Saitama, Japan), placed on glass slides (Fine Frost Micro Slide Grass; Matsunami Glass Ind., Ltd., Osaka, Japan).

Labeling:

Article Title: A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains
Article Snippet: The embedded sections were cut into 10 μm-slices using a microtome (Yamato Kohki Industrial Co., Ltd., PR-50, Saitama, Japan). .. Preparation of digoxigenin (DIG) labeled RNA probes or OsASNase1 , OsASNase2 , and OsGS1;1, and in situ hybridization analyses were performed as described by Ishiyama et al. ( , ).

Article Title: YAP-dependent necrosis occurs in early stages of Alzheimer’s disease and regulates mouse model pathology
Article Snippet: Sagittal or coronal sections (5 μm thickness) were obtained using a microtome (REM-710, Yamato Kohki Industrial Co., Ltd., Saitama, Japan). .. For multi-labeling, antibodies were labeled by Zenon Secondary Detection-Based Antibody Labeling Kits as follows: anti-calnexin, anti-RIP3, anti-pSer166-RIP1 and anti-pSer232-RIP3 (Zenon™ Alexa Fluor™ 555 Rabbit IgG Labeling Kit, Z-25305, Thermo Fisher Scientific, Waltham, MA, USA); anti-MAP2 (Zenon™ Alexa Fluor™ 647 Rabbit IgG Labeling Kit, Z-25308 and Zenon™ Alexa Fluor™ 488 Rabbit IgG Labeling Kit, Z-25302, Thermo Fisher Scientific, Waltham, MA, USA); anti-pSer345-MLKL (Zenon™ Alexa Fluor™ 647 Rabbit IgG Labeling Kit, Z25308 Thermo Fisher Scientific, Waltham, MA, USA); anti-amyloid β (Zenon™ Alexa Fluor™ 488 Mouse IgG1 Labeling Kit, Z-25002, Thermo Fisher Scientific, Waltham, MA, USA).

In Situ Hybridization:

Article Title: A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains
Article Snippet: Paragraph title: In situ hybridization for OsASNase and OsGS1;1 transcripts ... The embedded sections were cut into 10 μm-slices using a microtome (Yamato Kohki Industrial Co., Ltd., PR-50, Saitama, Japan).

Plasmid Preparation:

Article Title: Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist
Article Snippet: For immunostaining, serial section (30 μm thick) were cut using a microtome (REM-700; Yamato Kohki Industrial Co., Ltd., Saitama, Japan). .. After washing in 0.3% Triton X-100/PBS for 20 min, the sections were further incubated for 120 min with a biotinylated secondary antibody solution (1:250) and finally with an avidin–biotin peroxidase complex (Vector Laboratories Inc., Burlingame, CA, USA) for 120 min.

Software:

Article Title: Exosomes from conditioned media of bone marrow-derived mesenchymal stem cells promote bone regeneration by enhancing angiogenesis
Article Snippet: After 4 weeks of decalcification, samples were embedded in paraffin and sectioned at 4 μm thickness in the coronal plane using a microtome (REM-710, YAMATO KOHKI Industrial Co., Ltd., Saitama, Japan). .. Sections were dewaxed, rehydrated, stained with hematoxylin-eosin, and analyzed using a light microscope (FX630, OLYMPUS Co., Tokyo, Japan) and FLVFS-LS software (OLYMPUS Co.).

Concentration Assay:

Article Title: A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains
Article Snippet: The embedded sections were cut into 10 μm-slices using a microtome (Yamato Kohki Industrial Co., Ltd., PR-50, Saitama, Japan). .. For pre-hybridization, the solution was placed onto each slide containing the sections and it was incubated at 55 °C for 1 h. The solution was then replaced with the same solution containing DIG-labeled RNA probes at the concentration of 5 ng•ml−1 , and the slides were incubated at 55 °C for 16 h. The washing, detection and microscopic observation steps after hybridization were performed as described previously with slight modification (Sakurai et al. ; Ohashi et al. ).

Staining:

Article Title: A new dry eye mouse model produced by exorbital and intraorbital lacrimal gland excision
Article Snippet: Paragraph title: C57BL/6 WT mice samples for hematoxylin-eosin staining ... Approximately 3-µm thickness serial sections were cut using a sliding microtome (ROM-380; Yamato Kohki Industrial Co., Ltd., Saitama, Japan), placed on glass slides (Fine Frost Micro Slide Grass; Matsunami Glass Ind., Ltd., Osaka, Japan).

Article Title: Exosomes from conditioned media of bone marrow-derived mesenchymal stem cells promote bone regeneration by enhancing angiogenesis
Article Snippet: After 4 weeks of decalcification, samples were embedded in paraffin and sectioned at 4 μm thickness in the coronal plane using a microtome (REM-710, YAMATO KOHKI Industrial Co., Ltd., Saitama, Japan). .. Sections were dewaxed, rehydrated, stained with hematoxylin-eosin, and analyzed using a light microscope (FX630, OLYMPUS Co., Tokyo, Japan) and FLVFS-LS software (OLYMPUS Co.).

Article Title: Megalin Blockade with Cilastatin Suppresses Drug-Induced Nephrotoxicity
Article Snippet: .. From each tissue sample, 2- μ m-thick sections were cut using a microtome (REM-710; Yamato Kohki Industrial Co., Ltd.) and stained with PAS. .. In addition, 4- μ m-thick sections were used for immunofluorescence staining.

Article Title: YAP-dependent necrosis occurs in early stages of Alzheimer’s disease and regulates mouse model pathology
Article Snippet: Sagittal or coronal sections (5 μm thickness) were obtained using a microtome (REM-710, Yamato Kohki Industrial Co., Ltd., Saitama, Japan). .. Nuclei were stained with DAPI (0.2 μg/mL in PBS, D523, Dojindo Laboratories, Kumamoto, Japan).

Article Title: Inhibition of ghrelin-induced feeding in rats by pretreatment with a novel dual orexin receptor antagonist
Article Snippet: Paragraph title: Fos immunohistochemistry and double immunohistochemical staining for Fos and orexin-A ... For immunostaining, serial section (30 μm thick) were cut using a microtome (REM-700; Yamato Kohki Industrial Co., Ltd., Saitama, Japan).

Article Title: Effect of Single Injection of Recombinant Human Bone Morphogenetic Protein-2-Loaded Artificial Collagen-Like Peptide in a Mouse Segmental Bone Transport Model
Article Snippet: The resulting tissue was embedded in paraffin using an automatic tissue processor (Tissue-Tek VIP 6; Sakura Fine Tek, Tokyo, Japan) and sectioned through the femur's long axis at 3 μ m in the sagittal plane using a microtome (REM-710; Yamato Kohki industrial Co. Ltd., Saitama, Japan). .. All sections were then stained with hematoxylin and eosin (HE) and evaluated qualitatively.

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    Yamato Kohki Industrial Co Ltd microtome
    Microtome, supplied by Yamato Kohki Industrial Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Yamato Kohki Industrial Co Ltd
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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