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World Precision Instruments microtome
Microtome, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/World Precision Instruments
Average 91 stars, based on 3 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
91/100 stars

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Related Articles

Immunohistochemistry:

Article Title: Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption, et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption
Article Snippet: For immunohistochemical staining, postfixed brain tissues were dehydrated in a series of graded ethanol solutions and embedded in paraffin. .. For CSF tracer experiments, PFA postfixed forebrain tissues were sliced with a vibrating microtome (World Precision Instruments Inc, WPI; Cat. # NVSLM1‐436), at a thickness of 100 μm, and mounted onto gelatin‐coated slides in sequence.

In Vitro:

Article Title: Developmental Programming by Androgen Affects the Circadian Timing System in Female Mice 1
Article Snippet: Adult female PNA (n = 15), oil vehicle (n = 8), PAE (n = 17), and placebo (n = 8) mice of the PER2::LUC strain or untreated, random-cycling adult female PER2::LUC mice (for androgen treatment in vitro, n = 8) were euthanized 3 h before lights-off by excess CO2 exposure. .. The brain was sectioned in the coronal plane with a vibrating microtome (WPI, Inc.) at a thickness of 300 μm.

Isolation:

Article Title: Curcumin improves synaptic plasticity impairment induced by HIV-1gp120 V3 loop
Article Snippet: Paragraph title: Preparation of isolated rat hippocampus ... The hippocampi were dissected and sliced into 400 μm-thick coronal hippocampal sections using a vibrating microtome (World Precision Instruments, Sarasota, FL, USA).

Article Title: Developmental Programming by Androgen Affects the Circadian Timing System in Female Mice 1
Article Snippet: The brain was sectioned in the coronal plane with a vibrating microtome (WPI, Inc.) at a thickness of 300 μm. .. The ovary was isolated from the oviduct, and individual large follicles with a clear antrum were isolated.

Article Title: Noradrenergic Excitation of Magnocellular Neurons in the Rat Hypothalamic Paraventricular Nucleus via Intranuclear Glutamatergic Circuits
Article Snippet: The hypothalamus was blocked with a razor, and 400 μm hypothalamic slices were sectioned in the coronal plane using a vibrating microtome (World Precision Instruments, Sarasota, FL). .. In some experiments, the PVN was surgically isolated from the rest of the slice under a dissecting microscope using a scalpel.

Transferring:

Article Title: Nongenomic Glucocorticoid Inhibition via Endocannabinoid Release in the Hypothalamus: A Fast Feedback Mechanism
Article Snippet: The hypothalamus was blocked and the caudal surface of the block was glued to the chuck of a vibrating microtome (World Precision Instruments, Sarasota, FL). .. The pipette solution contained (in m m ): 120 K-gluconate, 10 KCl, 1 NaCl, 1 MgCl2 , 1 EGTA, 2 Mg-ATP, 0.3 Na-GTP, and 10 HEPES, pH adjusted to 7.3 with KOH.

Size-exclusion Chromatography:

Article Title: The anterior piriform cortex is sufficient for detecting depletion of an indispensable amino acid, showing independent cortical sensory function
Article Snippet: The rats were decapitated and their brains quickly removed and placed in chilled artificial cerebrospinal fluid (aCSF) for about 30 sec. After careful removal of the dura mater, a transverse cut was made approximately at the level of the optic chiasm. .. Slices (400μm) were cut from the submerged brain segment using a microtome (Vibroslice, World Precision Instruments [WPI], Sarasota, FL).

Microscopy:

Article Title: Noradrenergic Excitation of Magnocellular Neurons in the Rat Hypothalamic Paraventricular Nucleus via Intranuclear Glutamatergic Circuits
Article Snippet: The hypothalamus was blocked with a razor, and 400 μm hypothalamic slices were sectioned in the coronal plane using a vibrating microtome (World Precision Instruments, Sarasota, FL). .. In some experiments, the PVN was surgically isolated from the rest of the slice under a dissecting microscope using a scalpel.

Mouse Assay:

Article Title: Developmental Programming by Androgen Affects the Circadian Timing System in Female Mice 1
Article Snippet: Adult female PNA (n = 15), oil vehicle (n = 8), PAE (n = 17), and placebo (n = 8) mice of the PER2::LUC strain or untreated, random-cycling adult female PER2::LUC mice (for androgen treatment in vitro, n = 8) were euthanized 3 h before lights-off by excess CO2 exposure. .. The brain was sectioned in the coronal plane with a vibrating microtome (WPI, Inc.) at a thickness of 300 μm.

Article Title: TrkB as a potential synaptic tag and behavioral tag
Article Snippet: Coronal hippocampal slices (400 µm) were prepared from 8–10 week old male mice, in accordance with NIH guidelines. .. Briefly, hippocampal slices were cut using a vibrating microtome (NVSLM1, WPI Inc., Sarasota, FL) in ice-cold slicing buffer (in mM: 127 NaCl, 26 NaHCO3 , 1.2 KH2 PO4 , 1.9 KCl, 1.1 CaCl2 , 2 MgSO4 , 10 D-Glucose) bubbled with 95% O2 and 5% CO2 .

Slice Preparation:

Article Title: Castration modulates singing patterns and electrophysiological properties of RA projection neurons in adult male zebra finches
Article Snippet: Paragraph title: Slice preparation ... Coronal brain slices (250–300 µm thick) containing the RA were cut with a vibrating microtome (World Precision Instruments Inc., Sarasota, FL, USA) and collected in artificial cerebrospinal fluid (ACSF) that was warmed to 37 °C.

Article Title: Muscarinic Receptors Are Responsible for the Cholinergic Modulation of Projection Neurons in the Song Production Brain Nucleus RA of Zebra Finches
Article Snippet: Paragraph title: Animals and Brain Slice Preparation ... Coronal brain slices measuring 250–300 μm in thickness and containing RA were obtained using a vibrating microtome (NVSLM1, World Precision Instruments, USA).

Article Title: Carbachol-Induced Reduction in the Activity of Adult Male Zebra Finch RA Projection Neurons
Article Snippet: Paragraph title: 2.1. Animals and Slice Preparation ... Coronal brain slices (250 μ m thick) containing RA were obtained using a vibrating microtome (NVSLM1, World Precision Instruments, USA).

Article Title: The anterior piriform cortex is sufficient for detecting depletion of an indispensable amino acid, showing independent cortical sensory function
Article Snippet: Paragraph title: Brain slice preparation ... Slices (400μm) were cut from the submerged brain segment using a microtome (Vibroslice, World Precision Instruments [WPI], Sarasota, FL).

other:

Article Title: Modulation Effects of Cordycepin on Voltage-Gated Sodium Channels in Rat Hippocampal CA1 Pyramidal Neurons in the Presence/Absence of Oxygen
Article Snippet: Hippocampus was dissected free, and transverse hippocampal slices (400 μ m in thickness) were obtained using a vibrating microtome (NVSLM1, World Precision Instruments, USA).

Blocking Assay:

Article Title: The anterior piriform cortex is sufficient for detecting depletion of an indispensable amino acid, showing independent cortical sensory function
Article Snippet: The remaining ventral section of the forebrain, containing the APC, was then glued to an agar block with cyanoacrylate (Loctite, Rocky Hills, CT). .. Slices (400μm) were cut from the submerged brain segment using a microtome (Vibroslice, World Precision Instruments [WPI], Sarasota, FL).

Article Title: Nongenomic Glucocorticoid Inhibition via Endocannabinoid Release in the Hypothalamus: A Fast Feedback Mechanism
Article Snippet: .. The hypothalamus was blocked and the caudal surface of the block was glued to the chuck of a vibrating microtome (World Precision Instruments, Sarasota, FL). ..

Sequencing:

Article Title: Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption, et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption
Article Snippet: .. For CSF tracer experiments, PFA postfixed forebrain tissues were sliced with a vibrating microtome (World Precision Instruments Inc, WPI; Cat. # NVSLM1‐436), at a thickness of 100 μm, and mounted onto gelatin‐coated slides in sequence. .. 2.8 Immunohistochemistry Immunohistochemical staining was performed as previously described.

Staining:

Article Title: Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption, et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption
Article Snippet: For immunohistochemical staining, postfixed brain tissues were dehydrated in a series of graded ethanol solutions and embedded in paraffin. .. For CSF tracer experiments, PFA postfixed forebrain tissues were sliced with a vibrating microtome (World Precision Instruments Inc, WPI; Cat. # NVSLM1‐436), at a thickness of 100 μm, and mounted onto gelatin‐coated slides in sequence.

Immunofluorescence:

Article Title: Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption, et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption
Article Snippet: For double immunofluorescence, forebrains were dehydrated gradiently in 20% and 30% sucrose solutions for each 24 hours at 4°C. .. For CSF tracer experiments, PFA postfixed forebrain tissues were sliced with a vibrating microtome (World Precision Instruments Inc, WPI; Cat. # NVSLM1‐436), at a thickness of 100 μm, and mounted onto gelatin‐coated slides in sequence.

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    World Precision Instruments vibroslice microtome
    Vibroslice Microtome, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vibroslice microtome/product/World Precision Instruments
    Average 89 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    vibroslice microtome - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    91
    World Precision Instruments microtome
    Microtome, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/World Precision Instruments
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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